CN206579998U - A kind of portable profound hypothermia cell traffic device - Google Patents
A kind of portable profound hypothermia cell traffic device Download PDFInfo
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- CN206579998U CN206579998U CN201720119030.3U CN201720119030U CN206579998U CN 206579998 U CN206579998 U CN 206579998U CN 201720119030 U CN201720119030 U CN 201720119030U CN 206579998 U CN206579998 U CN 206579998U
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Abstract
A kind of portable profound hypothermia cell traffic device of the present utility model, including cover, tank body, filter and temperature display, tank wall are provided with handle, and are embedded with temperature display, and tank body inner bag is provided with filter, transported using vapor mode.Cell traffic device is in use, freeze-stored cell is placed on filter, and the liquid nitrogen of tank bottom maintains the profound hypothermia of tank interior, display screen shows the temperature of cell in real time, during cell traffic recovery, device is extracted and transported by operating personnel by handle as low-temperature receiver.This portable profound hypothermia cell traffic apparatus structure is simple, Portable light, and operator is easy to carry, improves the degree of accuracy and the motility rate of cell recovery, has filled up the blank of the such product of in the market.Meanwhile, the present apparatus uses gas phase profound hypothermia mode traffic program, has prevented the generation of cross pollution, and can show the temperature of cell in real time, improves the security of cell class product.
Description
Technical field
The utility model is related to biomedicine field, specifically a kind of portable profound hypothermia cell traffic device.
Background technology
Cell cryopreservation refers to cell being placed on profound hypothermia environment, reduction cell metabolism, so that cell long-period is preserved.At present,
By the way that cell is placed in into deep-bed drying in liquid nitrogen (reachable -196 DEG C), cell can be made to be temporarily disengaged from growth conditions and its is thin
Born of the same parents' characteristic is saved.A certain amount of cell is moderately preserved, can be avoided by microorganism pollution or other accidents
Cell is lost kind, be the conventional method in cell class laboratory.
When needing to use cell, by the way of " fast melt ".Concrete operations are:Take out and freeze from liquid nitrogen container
In pipe, 37 DEG C of water-baths of immersion, and shake makes it melt as early as possible frequently.Cell after thawing passes through the step such as centrifugation and washing,
Intracellular large biological molecule activity recovery can be made, normal metabolic processes are carried out, for cell culture experiments etc..
By freeze-stored cell from liquid nitrogen container be transferred to water-bath in carry out recovery when, it is necessary to fast operating, to reduce to cell
The influence of motility rate.In actual mechanical process, during a small amount of cell (≤5) of recovery, it is ensured that quick that cell is immersed into 37 DEG C
In warm water, recovery Cell viability is higher;When recover a large amount of cells when (such as 20), the retrieval of storage location and transhipment time compared with
Long, the residence time is longer at room temperature for freeze-stored cell, causes the reduction of recovery Cell viability, but also exist obscure, it is wrong
Risk by mistake.
In order to improve the motility rate of recovery cell, profound hypothermia state of the cell in location retrieval and transhipment is maintained, it is necessary to
Develop a kind of portable profound hypothermia cell traffic device.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, facilitate experimental implementation during cell recovery, carry
For a kind of portable profound hypothermia cell traffic device simple in construction, easy to use, safe.
The technical solution of the utility model is:A kind of portable profound hypothermia cell traffic device, including cover, tank body, every
Net and temperature display, tank wall are provided with handle, and are embedded with temperature display, and tank body inner bag is provided with filter.
Described cover manufactures for heat-barrier material, such as polystyrene foam plastics, plays heat-insulated and balance pressure work
With.
Described tank body manufactures for vacuum heat-insulation technique, plays a part of heat-insulated and ensures profound hypothermia.
Described tank body inner bag includes filter, and filter upper strata storage cell, lower floor's storage liquid nitrogen uses gas phase transporter
Case, prevents the generation of cross pollution.
Described temperature display is embedded in tank wall, and the components such as battery and alarming horn are contained in inside.Temperature display
The real time temperature of cell is shown, when temperature anomaly, alarming horn beeps, point out operating personnel to check.
The utility model has the advantages that:During cell recovery, the present apparatus can show the real-time of cell
Temperature, it is ensured that profound hypothermia cold chain is transported, and is reduced due to the adverse effect that temperature fluctuation is caused to cell, improves recovery thin
The motility rate of born of the same parents;Simultaneously using the design of wide-mouth footpath, facilitate the verification of cellular informatics, it is to avoid mistake and the generation obscured, can be once
Property complete large batch of cell traffic and resuscitative efforts, improve the accuracy and security of cell recovery.Using gas phase transhipment side
Case, while tank interior profound hypothermia is ensured, it is to avoid cross pollution.This portable profound hypothermia cell traffic tank has structure
Simply, Portable light, it is safe and efficient the features such as, filled up the blank that market is such product.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the enlarged drawing of the utility model filter.
Embodiment
1-2, is described in further detail to the utility model below in conjunction with the accompanying drawings.
A kind of portable profound hypothermia cell traffic device, including cover 1, handle 2, tank body 3, filter 4 and temperature display
6;
Cover 1 is buckled in the tank mouth of tank body 3, and the outer wall of tank body 3 sets handle 2, and the inner bag of tank body 3 is provided with wedge-shaped crab-bolt 5, the crab-bolt
5 are used for the positioning and support of filter 4;
Tank body 3 uses cylindrical-shaped structure, and tank body 3 uses double-layer vacuum inner bag;Using liquid nitrogen as low-temperature receiver, transhipment is kept
During profound hypothermia;
Cover 1 plays a part of insulation and reduction liquid nitrogen volatilization;The cover 1 is heat-insulated material, it is ensured that in tank body
The low temperature in portion;The diameter of cover 1 is smaller than tank mouth simultaneously, available for compensator inside and outside air pressure, prevents blast;
Filter 4 is placed in tank body 3, using stainless steel, netted braiding;
Temperature display 6 is embedded on the outer wall of tank body 3, and temperature display 6 is connected by connecting line with temperature sensor 7, temperature
Degree sensor 7 is arranged at filter 4;Temperature display 6 is used for the real time temperature for showing cell, can be with sound when temperature anomaly
Light form is alarmed;
The tank body is double-layer stainless steel material, and vacuum manufacture craft, vacuum is higher, plays heat-blocking action.
The outer wall of the tank body 3 is provided with Portable handle, facilitates operating personnel to transport;
The tank wall is provided with temperature display, the real time temperature for showing cell;
The tank body inner bag is provided with the crab-bolt for being used for supporting filter.
Cover 1 is high-density polystyrene foamed plastics material (Expanded Polystyrene, abbreviation EPS), heat-insulated
Ability is good, and the profound hypothermia of tank interior has been effectively ensured;While cover diameter 5~10mm smaller than tank mouth, in compensator body
Outer air pressure.
Tank body 3 is double-layer stainless steel material, vacuum manufacture craft, vacuum≤5x10-5Pa, heat insulation capacity is strong, effectively protects
The profound hypothermia of tank interior is demonstrate,proved.
Filter 4 is stainless steel, and freeze-stored cell is positioned over upper strata, and lower floor is liquid nitrogen, effectively maintains tank interior
Profound hypothermia (≤- 150 DEG C), while avoiding cross pollution.
Temperature display 6 is connected with temp probe 7, can show the real time temperature of cell.Display screen includes battery and report
The components such as alert loudspeaker, when temperature anomaly, the prompting operating personnel that beep check.
This portable profound hypothermia cell traffic tank in use, liquid nitrogen filling first is to 8~10cm height (being no more than filter),
Stand after 1~2h, when temperature display numerical value is less than -150 DEG C, show that the device can be used;Secondly taken from liquid nitrogen container
Go out cell, be positioned in transhipment tank;Under profound hypothermia protection, the information of cell is checked, it is ensured that accurate;Finally it is transported to water
By bath, recovery cell in batches.
Above is preferred embodiment of the present utility model, all changes made according to technical solutions of the utility model are produced
Function without departing from technical solutions of the utility model scope when, belong to protection domain of the present utility model.
Claims (4)
1. a kind of portable profound hypothermia cell traffic device, it is characterised in that:It is aobvious including cover, handle, tank body, filter and temperature
Display screen;
Cover is buckled in tank body tank mouth, and tank wall sets handle, and tank body inner bag is provided with wedge-shaped crab-bolt, and the crab-bolt is used for filter
Positioning and support;
Tank body uses cylindrical-shaped structure, and tank body uses double-layer vacuum inner bag;Using liquid nitrogen as low-temperature receiver, keep in transport process
Profound hypothermia;
Cover plays a part of insulation and reduction liquid nitrogen volatilization;The cover be heat-insulated material, it is ensured that tank interior it is low
Temperature;Cover diameter is smaller than tank mouth simultaneously, available for compensator inside and outside air pressure, prevents blast;
Filter is placed in tank body, using stainless steel, netted braiding;
Temperature display is embedded on tank wall, and temperature display is connected by connecting line with temperature sensor, temperature sensor
It is arranged at filter;Temperature display is used for the real time temperature for showing cell, when temperature anomaly, can be alarmed with acoustooptic form.
2. a kind of portable profound hypothermia cell traffic device according to claim 1, it is characterised in that:
Cover is high-density polystyrene foamed plastics material, and heat-insulating capability is good, and the profound hypothermia of tank interior has been effectively ensured;Together
When cover diameter 5~10mm smaller than tank mouth, for compensator inside and outside air pressure.
3. a kind of portable profound hypothermia cell traffic device according to claim 1, it is characterised in that:
Tank body is double-layer vacuum stainless steel inner container, vacuum≤5x10-5Pa, heat insulation capacity is strong, and tank interior has been effectively ensured
Profound hypothermia.
4. a kind of portable profound hypothermia cell traffic device according to claim 1, it is characterised in that:
Filter is stainless steel, and freeze-stored cell is positioned over upper strata, and lower floor is liquid nitrogen, and the effective depth for maintaining tank interior is low
Temperature, temperature≤- 150 DEG C, it is to avoid cross pollution.
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CN201720119030.3U CN206579998U (en) | 2017-02-09 | 2017-02-09 | A kind of portable profound hypothermia cell traffic device |
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CN201720119030.3U CN206579998U (en) | 2017-02-09 | 2017-02-09 | A kind of portable profound hypothermia cell traffic device |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109128104A (en) * | 2018-08-09 | 2019-01-04 | 云南科威液态金属谷研发有限公司 | A kind of preparation facilities of low melting point foam metal, method and low melting point foam metal |
CN109699632A (en) * | 2018-12-26 | 2019-05-03 | 北京优迅医学检验实验室有限公司 | Cell-preservation liquid, store method and its application |
WO2020107650A1 (en) * | 2018-11-28 | 2020-06-04 | 上海原能细胞生物低温设备有限公司 | Cryogenic transport device |
-
2017
- 2017-02-09 CN CN201720119030.3U patent/CN206579998U/en active Active
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109128104A (en) * | 2018-08-09 | 2019-01-04 | 云南科威液态金属谷研发有限公司 | A kind of preparation facilities of low melting point foam metal, method and low melting point foam metal |
CN109128104B (en) * | 2018-08-09 | 2021-09-07 | 云南科威液态金属谷研发有限公司 | Preparation device and method of low-melting-point foam metal and low-melting-point foam metal |
WO2020107650A1 (en) * | 2018-11-28 | 2020-06-04 | 上海原能细胞生物低温设备有限公司 | Cryogenic transport device |
CN109699632A (en) * | 2018-12-26 | 2019-05-03 | 北京优迅医学检验实验室有限公司 | Cell-preservation liquid, store method and its application |
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