CN206556971U - A kind of malachite green molecular engram multilayer solid phase extraction column - Google Patents
A kind of malachite green molecular engram multilayer solid phase extraction column Download PDFInfo
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- CN206556971U CN206556971U CN201720237346.2U CN201720237346U CN206556971U CN 206556971 U CN206556971 U CN 206556971U CN 201720237346 U CN201720237346 U CN 201720237346U CN 206556971 U CN206556971 U CN 206556971U
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- China
- Prior art keywords
- filler
- malachite green
- pieces
- sieve plates
- solid phase
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Abstract
The utility model discloses a kind of malachite green molecular engram multilayer solid phase extraction column, including：Splitter and it is arranged at two layers of filler in splitter between dismountable three pieces of sieve plates in upper, middle and lower and three pieces of sieve plates；It is described it is upper, in filler between two pieces of sieve plates be quartz sand；Filler in described, between lower two pieces of sieve plates is malachite green molecular imprinting filler.The splitter is including hollow polypropylene plastics stock column and is arranged at piston movable up and down in polypropylene plastics stock column；Filler is arranged on the lower section of polypropylene plastics stock column inner carrier between three pieces of sieve plates and three pieces of sieve plates；Column cap is provided with below the polypropylene plastics stock column, and is connected with lower screening deck.The utility model can effectively remove the fat in sample when handling animal tissue etc. and also having the more sample of fat by the quartz sand layer on upper strata, the effective specificity capture malachite green molecule of lower floor's molecular engram filler, be easy to it is efficient, accurately detect product.
Description
Technical field
The utility model is related to solid phase extraction techniques neighborhood, more particularly to a kind of malachite green molecular engram multilayer solid phase extraction
Take pillar.
Background technology
Malachite green is widely used in preventing and treating the saprolegniasis of all kinds of aquatic livestocks, branchiomycosis and unguiculus parasitosis etc..
But malachite green and its metabolite easily high residue in aquatic products body, easily cause carcinogenic, teratogenesis and mutagenesis etc. after people is edible
Phenomenon.
In the detection and analysis work of chemical example, particularly nature sample, most common problem is that the component of sample is big
It is all excessively complicated when many, it is impossible to bad by chromatogram progress Direct Analysis, or Direct Analysis effect.Accordingly, it would be desirable to by right
Chemical example carries out pre-treatment to realize the separation of determinand, then carries out chromatography.Locate before chemical example the most frequently used at present
Reason technology is SPE (SPE) technology, i.e., realize suction by the special component and the affinity of solid extracting agent in component
Attached extraction, so as to realize the separation of special component.
SPE, is combined by liquid-solid extraction and liquid chromatography technology and developed, and is mainly used in the separating of sample, pure
Change and concentrate.To be widely applied to sample pretreatment process the features such as its is simple to operate, reproducible, accumulation rate is high.It is commercial
Solid-phase extraction column can be divided into positive extraction, reversed phase extraction, ion exchange extraction and sorption extraction according to the difference of adsorbent property
Etc. several, it mainly passes through electrostatic force, ion exchange power and the Hyarogen-bonding between solid phase filler and separation object
Deng separation, purifying and enrichment of the realization to sample component.Therefore, seem particularly important for solid extracting agent selection.Solid phase extracts
Take particular functional group, the pore-size distribution on solid extracting agent surface and specific surface area that the polarity of agent, solid extracting agent possesses etc.
Characteristic, directly determines the extraction efficiency and selectivity of whole SPE processes.
General SPE is using following operation：Activation-loading-elution-elution.Conventional solid-phase extraction device is at place
When managing sample more containing fat as animal tissue etc., the easy blocking portion filler hole during Solid phase extraction,
Single pillar positive pressure operation is wasted time and energy and reappearance is difficult to ensure that.Filler used in other routine can not be directed to malachite green
Molecule carries out specific capture, but the molecule of many classes can be adsorbed, and target molecule absorption is thus have impact on indirectly
Effective percentage.
Therefore, the prior art is defective, it is necessary to improve.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art there is provided a kind of malachite green molecular engram multilayer solid phase
Extract pillar.
The technical solution of the utility model is as follows：A kind of malachite green molecular engram multilayer solid phase extraction column, including：Point
From post and upper and lower two layers of the filler being arranged in splitter；The upper strata filler is quartz sand layer；Lower floor's filler is peacock
Malachite green molecular imprinting filler.
Further, dismountable upper, middle and lower-ranking sieve plate is additionally provided with the splitter；The upper strata sieve plate is set
Above quartz sand layer, the middle level sieve plate is arranged between quartz sand layer and malachite green molecular imprinting filler, the lower floor
Sieve plate is arranged on below malachite green molecular imprinting filler.
Further, the splitter include hollow polypropylene plastics stock column and be arranged in polypropylene plastics stock column can above and below
The piston of activity；Filler is arranged on the lower section of polypropylene plastics stock column inner carrier between three pieces of sieve plates and three pieces of sieve plates；It is described
Column cap is provided with below polypropylene plastics stock column, and is connected with lower screening deck.
Using such scheme, the utility model provides a kind of malachite green molecular engram multilayer solid phase extraction column, at place
When there be fat more sample in reason animal tissue etc., the fat in sample can be effectively removed by the quartz sand layer on upper strata, under
The effective specificity capture malachite green molecule of layer molecular engram filler, and elution requirement is gentle, eluent can directly be used to
Quick detection product, be easy to it is efficient, accurately detect product composition.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
To enable above-mentioned purpose of the present utility model, feature and advantage more obvious understandable, below in conjunction with the accompanying drawings to this
The embodiment of utility model is described in detail.Elaborate many details in order to abundant in the following description
Understand the utility model.But the utility model can be implemented with being much different from other manner described here, this area
Technical staff can do similar improvement in the case of without prejudice to the utility model intension, therefore the utility model is not by following public affairs
The limitation for the specific implementation opened.
Shown in reference picture 1, the utility model provides a kind of malachite green molecular engram multilayer solid phase extraction column, including：
Splitter 1 and it is arranged at two layers of filler in splitter 1 between dismountable three pieces of sieve plates 3 in upper, middle and lower and three pieces of sieve plates 3.
The splitter 1 is including hollow polypropylene plastics stock column 11 and is arranged at piston movable up and down in polypropylene plastics stock column 11
12；Filler is arranged on the lower section of polypropylene plastics stock column inner carrier 12 between three pieces of sieve plates 3 and three pieces of sieve plates 3；Described poly- third
The lower section of alkene plastics post 11 is provided with column cap 13, and is connected with lower screening deck 3.The sieve plate 3 is porous aluminum oxide sieve plate 3；It is described
Above, the filler between two pieces of sieve plates 3 is quartz sand layer 21；Filler in described, between lower two pieces of sieve plates 3 is malachite green point
Sub- marking filler 22.
Wherein, the preparation method of the malachite green molecular imprinting filler 22, comprises the following steps：1st, by hydrophobicity list
Body, malachite green are mixed with oil-soluble initiator, and are sufficiently stirred in oil phase storage filling, form dispersed oil phase fluid；2、
Stabilizer and water are added in a kettle., and dispersed with stirring forms uniform aqueous phase solution；3rd, pressurization makes the oil phase fluid dispersion
Into in the aqueous phase solution, uniform O/W types suspension is formed；4th, constant temperature polymerisation synthesizes the malachite green of uniform particle diameter
Polymer composite microsphere；5th, template molecule malachite green is removed, solid phase extraction filler is obtained.It is noted that this method is adopted
Disperseed with the scattered mechanical agitation that substitutes of jet flow, oil phase fluid is dispersed and polymerisation is separately carried out.Pass through injection
Oil phase fluid is uniformly crushed and stable O/W type suspension is formed in aqueous phase, polymerisation can obtain uniform particle diameter
Polymer microballoon, removes template molecule, finally obtains malachite green molecular imprinting filler 22.
The malachite green molecular imprinting filler 22 obtained by this method has the following advantages that：The solid phase extraction filler of preparation
Uniform particle diameter, packing material size size is adjustable；The solid phase extraction filler chemical stability of preparation is good；The solid phase extraction filler choosing of preparation
Selecting property is high, and the rate of recovery is high；It is easy to amplification industrialization.
Filler between three pieces of sieve plates 3 of the utility model has two layers, and upper strata quartz sand layer 21 effectively removes the fat in sample
Fat, it is to avoid the easy hole of blocking portion sieve plate 3 during Solid phase extraction；Lower floor's malachite green molecular imprinting filler 22 has
The specificity capture malachite green molecule of effect, and elution requirement is gentle, eluent can directly be used to quick detection product, such as glue
Body gold test paper strip and ELISA experiments.Pre-treatment is carried out to chemical example using the solid phase extraction column, can be by system to be measured
Every component effectively fix, so as to realize efficient separating effect, it is to avoid target components can not be by follow-up detection
Measure or actual value can not be detected and existed data distortion the problem of.
In summary, the utility model provides a kind of malachite green molecular engram multilayer solid phase extraction column, dynamic in processing
When thing tissue etc. also has fat more sample, the fat in sample, lower floor point can be effectively removed by the quartz sand layer on upper strata
The effective specificity capture malachite green molecule of sub- trace filler, be easy to it is efficient, accurately detect product.
Preferred embodiment of the present utility model is these are only, the utility model is not limited to, it is all in this practicality
Any modifications, equivalent substitutions and improvements made within new spirit and principle etc., should be included in guarantor of the present utility model
Within the scope of shield.
Claims (3)
1. a kind of malachite green molecular engram multilayer solid phase extraction column, it is characterised in that including：Splitter and it is arranged at separation
Upper and lower two layers of filler in post；The upper strata filler is quartz sand layer；Lower floor's filler is that malachite green molecular imprinting is filled out
Material.
2. malachite green molecular engram multilayer solid phase extraction column according to claim 1, it is characterised in that the separation
Dismountable upper, middle and lower-ranking sieve plate is additionally provided with post；The upper strata sieve plate is arranged on above quartz sand layer, the middle level sieve
Plate is arranged between quartz sand layer and malachite green molecular imprinting filler, and lower floor's sieve plate is arranged on malachite green molecular imprinting
Below filler.
3. malachite green molecular engram multilayer solid phase extraction column according to claim 2, it is characterised in that the separation
Post is including hollow polypropylene plastics stock column and is arranged at piston movable up and down in polypropylene plastics stock column；Three pieces of sieve plates and
Filler is arranged on the lower section of polypropylene plastics stock column inner carrier between three pieces of sieve plates；Column cap is provided with below the polypropylene plastics stock column,
And connected with lower screening deck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720237346.2U CN206556971U (en) | 2017-03-10 | 2017-03-10 | A kind of malachite green molecular engram multilayer solid phase extraction column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720237346.2U CN206556971U (en) | 2017-03-10 | 2017-03-10 | A kind of malachite green molecular engram multilayer solid phase extraction column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206556971U true CN206556971U (en) | 2017-10-13 |
Family
ID=60363965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720237346.2U Expired - Fee Related CN206556971U (en) | 2017-03-10 | 2017-03-10 | A kind of malachite green molecular engram multilayer solid phase extraction column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206556971U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108905287A (en) * | 2018-08-22 | 2018-11-30 | 佛山科学技术学院 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
-
2017
- 2017-03-10 CN CN201720237346.2U patent/CN206556971U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108905287A (en) * | 2018-08-22 | 2018-11-30 | 佛山科学技术学院 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171013 |