CN108905287A - One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green - Google Patents
One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green Download PDFInfo
- Publication number
- CN108905287A CN108905287A CN201810958294.7A CN201810958294A CN108905287A CN 108905287 A CN108905287 A CN 108905287A CN 201810958294 A CN201810958294 A CN 201810958294A CN 108905287 A CN108905287 A CN 108905287A
- Authority
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- China
- Prior art keywords
- loading pipe
- sieve plate
- section
- flesh
- malachite green
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 title claims abstract description 23
- 229940107698 malachite green Drugs 0.000 title claims abstract description 23
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 19
- 238000002414 normal-phase solid-phase extraction Methods 0.000 title claims abstract description 16
- 230000008602 contraction Effects 0.000 claims description 9
- 238000000605 extraction Methods 0.000 abstract description 16
- 230000006872 improvement Effects 0.000 description 11
- 239000000945 filler Substances 0.000 description 10
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000011218 segmentation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229910000464 lead oxide Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- YEXPOXQUZXUXJW-UHFFFAOYSA-N oxolead Chemical compound [Pb]=O YEXPOXQUZXUXJW-UHFFFAOYSA-N 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/12—Meat; Fish
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses one kind for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, it include tube body, the bottom of the tube body is provided with extracting tube group, the bottom of the extracting tube group is provided with outlet tube, the extracting tube group includes no less than one loading pipe, the loading pipe is to be detachably connected with the tube body, the loading pipe is to be detachably connected with the outlet tube, upper sieve plate and lower sieve plate are provided in the loading pipe, the lower sieve plate is connected by a snap in the loading pipe, the upper sieve plate is set to the top of the lower sieve plate, the present invention can be according to the difference of flesh of fish sample, extraction times are different, it can freely arrange in pairs or groups and assemble multiple loading pipes, it is more flexible and convenient when use.
Description
Technical field
The present invention relates to a kind of extraction equipments, more particularly to one kind is for measuring the remaining solid phase extraction of flesh of fish Malachite Green
Take column.
Background technique
Malachite green is widely used in preventing and treating the saprolegniasis of all kinds of aquatic livestocks, branchiomycosis and unguiculus parasitosis etc.,
But malachite green and its metabolite high residue easily in aquatic products body easily cause carcinogenic, teratogenesis and mutagenesis etc. after people is edible
Phenomenon, general Solid Phase Extraction is using following operation:Activation-loading-elution-elution is needed in actual extraction to sample
The number of extraction will according to the actual situation depending on, different samples in order to extract malachite green, to required for sample extract
Number would also vary from, and original extraction column is due to the architectural characteristic of its own, each extraction column can extraction times according to
The construction of its own and determine, when use, is inflexible, and adds filler and be also more troublesome, and practical operation can be very inconvenient.
Summary of the invention
The technical problem to be solved by the present invention is to:It needs a kind of for measuring the remaining Solid Phase Extraction of flesh of fish Malachite Green
Column can freely set its extraction times according to the difference of sample, not limited by the structure of extraction column script.
The solution that the present invention solves its technical problem is:One kind is for measuring the remaining solid phase of flesh of fish Malachite Green
Extraction column includes tube body, and the bottom of the tube body is provided with extracting tube group, and the bottom of the extracting tube group is provided with out liquid
Pipe, the extracting tube group include no less than one loading pipe, and the loading pipe is to be detachably connected with the tube body, institute
It is to be detachably connected that loading pipe, which is stated, with the outlet tube, and upper sieve plate and lower sieve plate, the lower sieve are provided in the loading pipe
Plate is connected by a snap in the loading pipe, and the upper sieve plate is set to the top of the lower sieve plate.
The present invention provides one kind for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, includes tube body, bottom
It is provided with extracting tube group, the bottom of extracting tube group is provided with outlet tube, and extracting tube group includes no less than one loading pipe, fills out
Expects pipe is to be detachably connected with tube body, and loading pipe is to be detachably connected with outlet tube, is provided with upper sieve plate in each loading pipe
With lower sieve plate, lower sieve plate is connected by a snap in loading pipe, can be easy to disassemble, and upper sieve plate is set to the top of lower sieve plate,
In actual mechanical process, need to be added the difference of the number of plies of filler, settable difference number in extraction process according to different samples
The loading pipe of amount can place one in a loading pipe to solve the problems, such as repeatedly to extract between upper sieve plate and lower sieve plate
Packing layer such as needs three kinds of filler extractions three times, then is connected as an extracting tube group using three loading pipes, when use more
Add flexibly, therefore, an extracting tube is divided into multistage by the present invention, and integral disassembly, assembly is convenient, can be increased according to different needs and be assembled
Different loading pipes, thus meet the requirement for needing extraction times different when measuring malachite green vestigial to flesh of fish sample, and
It can facilitate and filler is cleaned, directly disassemble loading pipe segmentation, be cleaned compared with traditional extracting tube more quick
It is convenient.
As a further improvement of the above technical scheme, the loading pipe includes sequentially connected upper fixation from top to bottom
Section, contraction section, lower fixed section, the outer diameter of the contraction section from top to bottom gradually taper up.
As a further improvement of the above technical scheme, the loading pipe is provided with several, fixed section interior
Peripheral wall is provided with internal thread segment, and the periphery wall of the lower fixed section is provided with external thread section, the internal thread segment and the outer spiral shell
Line section, which corresponds, to be cooperatively connected.
As a further improvement of the above technical scheme, the lower, outer perimeter wall of the tube body is provided with external thread section, described
The internal perisporium of upper fixed section is provided with internal thread segment, and the external thread section and the internal thread segment, which cooperate, to be connected.
As a further improvement of the above technical scheme, the top internal perisporium of the outlet tube is provided with internal thread segment, institute
The periphery wall for stating lower fixed section is provided with external thread section, and the internal thread segment and the external screw thread, which cooperate, to be connected.
As a further improvement of the above technical scheme, the periphery wall of the lower sieve plate is evenly arranged with several card slots,
The position that the loading pipe corresponds to the card slot is provided with fixture block, and the fixture block and the card slot, which correspond, to be cooperatively connected.
As a further improvement of the above technical scheme, piston rod is provided in the tube body.
Beneficial effect using above-mentioned further scheme is:Integrated operation is more flexible and convenient, between every pipe connection not
It is more preferable to be easy airtightness, it is not easy to leakage problem occur.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is a part of the embodiments of the present invention, rather than is all implemented
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is whole interior structural schematic diagram of the invention.
In figure:It is fixed under sieve plate, the upper fixed section of 5-, 6- contraction section, 7- under 1- tube body, 2- outlet tube, 3- upper sieve plate, 4-
Section, 8- piston rod.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to design of the invention, specific structure and generation clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of the embodiment, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art are not being paid
Other embodiments obtained, belong to the scope of protection of the invention under the premise of creative work.In addition, be previously mentioned in text
All connection/connection relationships not singly refer to that component directly connects, and referring to can be added deduct according to specific implementation situation by adding
Few couple auxiliary, to form more preferably coupling structure.Each technical characteristic in the invention, in not conflicting conflict
Under the premise of can be with combination of interactions.
Referring to Fig.1, a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, it include tube body 1, the pipe
The bottom of body 1 is provided with extracting tube group, and the bottom of the extracting tube group is provided with outlet tube 2, and the extracting tube group includes not
Loading pipe less than one, the loading pipe are to be detachably connected with the tube body 1, the loading pipe and the outlet tube 2
To be detachably connected, upper sieve plate 3 and lower sieve plate 4 are provided in the loading pipe, the lower sieve plate 4 is connected by a snap in institute
It states in loading pipe, the upper sieve plate 3 is set to the top of the lower sieve plate 4.
It is used to measure the remaining solid-phase extraction column of flesh of fish Malachite Green it can be seen from the above, the present invention provides one kind, including
There is tube body 1, bottom is provided with extracting tube group, and the bottom of extracting tube group is provided with outlet tube 2, and extracting tube group includes no less than one
A loading pipe, loading pipe are to be detachably connected with tube body 1, and loading pipe is to be detachably connected with outlet tube 2, in each loading pipe
It is provided with upper sieve plate 3 and lower sieve plate 4, lower sieve plate 4 is connected by a snap in loading pipe, can be easy to disassemble, upper sieve plate 3 is arranged
In the top of lower sieve plate 4, in the actual operation process, need to be added the number of plies of filler in extraction process according to different samples
Difference, the loading pipe of settable different number, to solve the problems, such as repeatedly to extract, in a loading pipe can upper sieve plate 3 with
A packing layer is placed between lower sieve plate 4, is such as needed three kinds of filler extractions three times, is then connected as one using three loading pipes
A extracting tube group, when use, are more flexible, and therefore, an extracting tube is divided into multistage by the present invention, and integral disassembly, assembly is convenient, can basis
Different needs increase the different loading pipe of assembling, need to extract when to meeting to flesh of fish sample measurement malachite green vestigial secondary
The different requirement of number, and can facilitate and filler is cleaned, directly loading pipe segmentation is disassembled, with traditional extracting tube
It is more efficient and convenient compared to cleaning.
Be further used as preferred embodiment, the loading pipe include from top to bottom sequentially connected upper fixed section 5,
The outer diameter of contraction section 6, lower fixed section 7, the contraction section 6 from top to bottom gradually tapers up, and such structure may make that loading pipe is
General structure can be conveniently connected through a screw thread between each loading pipe, it is preferred that upper fixed section 5, contraction section 6, lower fixed section
7 can be an integral molding structure, it is preferred that upper sieve plate 3 and lower sieve plate 4 may be provided in contraction section 6, and solution to be extracted is upper solid
Determine in section 5, after being extracted by the packing layer in contraction section 6, solution extracted is flowed into lower fixed section 7.
As a further improvement of the above technical scheme, the loading pipe is provided with several, fixed section 5 interior
Peripheral wall is provided with internal thread segment, and the periphery wall of the lower fixed section 7 is provided with external thread section, the internal thread segment and the outer spiral shell
Line section, which corresponds, to be cooperatively connected, and therefore, the interconnection between loading pipe can pass through a loading pipe and another loading pipe
External thread section and internal thread segment be cooperatively connected, to realize that quick splicing between loading pipe assembles, and loading pipe this
When structure setting may make lower section loading pipe and upper section loading pipe to splice, lower section loading pipe is nested into upper section filler bottom of the tube, and
It is not the top that upper section loading pipe is nested into lower section loading pipe, there are when hydraulic pressure in loading pipe, water flow is not easily accessed phase
In the thread segment mutually cooperated, airtightness is more preferable.
As a further improvement of the above technical scheme, the lower, outer perimeter wall of the tube body 1 is provided with external thread section, described
The internal perisporium of upper fixed section 5 is provided with internal thread segment, and the external thread section and the internal thread segment, which cooperate, to be connected, equally
, the cooperation of this structure can facilitate the mutual cooperation between loading pipe and tube body 1 to connect.
As a further improvement of the above technical scheme, the top internal perisporium of the outlet tube 2 is provided with internal thread segment, institute
The periphery wall for stating lower fixed section 7 is provided with external thread section, and the internal thread segment and the external screw thread, which cooperate, to be connected, equally
, the cooperation of this structure can facilitate the mutual cooperation between loading pipe and outlet tube 2 to connect.
As a further improvement of the above technical scheme, the periphery wall of the lower sieve plate 4 is evenly arranged with several card slots,
The position that the loading pipe corresponds to the card slot is provided with fixture block, and the fixture block and the card slot, which correspond, to be cooperatively connected,
Preferably, fixture block and card slot it is settable there are three, by the cooperation of fixture block and card slot, can facilitate to lower 4 Fast Installation of sieve plate
To loading pipe.
As a further improvement of the above technical scheme, it is provided with piston rod 8 in the tube body 1, piston rod 8 can be in tube body
1 top moves up and down, and to pressurizeing in tube body 1, accelerates the process of extraction, it is preferred that optional for the extractant of malachite green
With pure water or acetonitrile, quartz sand layer, lead oxide, malachite green molecular imprinting filler etc. also can be selected and be used as filler.
Better embodiment of the invention is illustrated above, but the invention is not limited to the implementation
Example, those skilled in the art can also make various equivalent modifications on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (7)
- It include tube body (1) 1. one kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, it is characterised in that:Institute The bottom for stating tube body (1) is provided with extracting tube group, and the bottom of the extracting tube group is provided with outlet tube (2), the extracting tube group It include no less than one loading pipe, the loading pipe is to be detachably connected with the tube body (1), the loading pipe and institute Stating outlet tube (2) is to be detachably connected, and upper sieve plate (3) and lower sieve plate (4), the lower sieve plate are provided in the loading pipe (4) it is connected by a snap in the loading pipe, the upper sieve plate (3) is set to the top of the lower sieve plate (4).
- 2. according to claim 1 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:The loading pipe includes from top to bottom sequentially connected upper fixed section (5), contraction section (6), lower fixed section (7), the receipts The outer diameter of contracting section (6) from top to bottom gradually tapers up.
- 3. according to claim 2 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:The loading pipe is provided with several, and the internal perisporium of fixed section (5) is provided with internal thread segment, the lower fixed section (7) periphery wall is provided with external thread section, and the internal thread segment and the external thread section, which correspond, to be cooperatively connected.
- 4. according to claim 2 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:The lower, outer perimeter wall of the tube body (1) is provided with external thread section, and the internal perisporium of fixed section (5) is provided with internal screw thread Section, the external thread section and the internal thread segment, which cooperate, to be connected.
- 5. according to claim 2 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:The top internal perisporium of the outlet tube (2) is provided with internal thread segment, and the periphery wall of the lower fixed section (7) is provided with outer spiral shell Line section, the internal thread segment and the external screw thread, which cooperate, to be connected.
- 6. according to claim 1 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:The periphery wall of the lower sieve plate (4) is evenly arranged with several card slots, and the position that the loading pipe corresponds to the card slot is all provided with It is equipped with fixture block, the fixture block and the card slot, which correspond, to be cooperatively connected.
- 7. according to claim 1 a kind of for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green, feature exists In:Piston rod (8) are provided in the tube body (1).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810958294.7A CN108905287A (en) | 2018-08-22 | 2018-08-22 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810958294.7A CN108905287A (en) | 2018-08-22 | 2018-08-22 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
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CN201810958294.7A Pending CN108905287A (en) | 2018-08-22 | 2018-08-22 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112494992A (en) * | 2020-11-02 | 2021-03-16 | 河北伊莱莎生物技术有限公司 | Dioxin pretreatment purification combined column and preparation method and application thereof |
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CN102974129A (en) * | 2012-12-10 | 2013-03-20 | 浙江省海洋水产研究所 | Multi-stage combined type multi-wall carbon nano tube solid phase extraction column |
CN106481817A (en) * | 2016-12-12 | 2017-03-08 | 苏州楚博生物技术有限公司 | Butt sealing assembly for tandem chromatographic column |
CN206192956U (en) * | 2016-11-21 | 2017-05-24 | 天津农学院 | Drug residue detects and uses extraction device |
CN206450638U (en) * | 2016-09-30 | 2017-08-29 | 珠海赛隆药业股份有限公司 | The GM1 detection chromatographic column of high-purity |
CN206556971U (en) * | 2017-03-10 | 2017-10-13 | 深圳市瑞赛生物技术有限公司 | A kind of malachite green molecular engram multilayer solid phase extraction column |
CN208852462U (en) * | 2018-08-22 | 2019-05-14 | 佛山科学技术学院 | One kind is for measuring the remaining solid-phase extraction column of flesh of fish Malachite Green |
-
2018
- 2018-08-22 CN CN201810958294.7A patent/CN108905287A/en active Pending
Patent Citations (7)
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CN101879381A (en) * | 2005-07-26 | 2010-11-10 | 诺华赛公司 | The method and apparatus of separating mixture component |
CN102974129A (en) * | 2012-12-10 | 2013-03-20 | 浙江省海洋水产研究所 | Multi-stage combined type multi-wall carbon nano tube solid phase extraction column |
CN206450638U (en) * | 2016-09-30 | 2017-08-29 | 珠海赛隆药业股份有限公司 | The GM1 detection chromatographic column of high-purity |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112494992A (en) * | 2020-11-02 | 2021-03-16 | 河北伊莱莎生物技术有限公司 | Dioxin pretreatment purification combined column and preparation method and application thereof |
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