CN206502762U - Prepare the process system of carnosine - Google Patents
Prepare the process system of carnosine Download PDFInfo
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- CN206502762U CN206502762U CN201720124700.0U CN201720124700U CN206502762U CN 206502762 U CN206502762 U CN 206502762U CN 201720124700 U CN201720124700 U CN 201720124700U CN 206502762 U CN206502762 U CN 206502762U
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- carnosine
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- alanine
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Abstract
The utility model is related to a kind of process system for preparing carnosine, including the first reactor（1）, the second reactor（2）, the first centrifuge（3）, ultrafiltration apparatus（5）, nanofiltration equipment（6）, crystallization tank（7）, the second centrifuge（8）；Also include methanol basin（9）, thionyl chloride basin（10）, beta Alanine basin（11）, the methanol basin（9）, thionyl chloride basin（10）, beta Alanine basin（11）Positioned at first reactor（1）Upstream, and respectively with first reactor（1）Pipeline is connected；Also include L histidine basins（12）, deionized water basin（13）, alkaline solution basin（14）；The L histidines basin（12）, deionized water basin（13）, alkaline solution basin（14）Positioned at second reactor（2）Upstream, and respectively with second reactor（2）Pipeline is connected.
Description
Technical field
The utility model is related to chemical process, particularly prepares the process unit of carnosine.
Background technology
Carnosine is by-the dipeptides that constitutes of alanine and L-Histidine（ß-Ala-L-His）, carnosine have it is anti-oxidant, anti-from
By base and anti-aging effects, therefore carnosine is applied to cosmetic health product field.In recent years, there are some researches show carnosine is old to initial stage
Year cataract, unripe cataract, and other concurrent cataracts have therapeutic action（Effective percentage more than 80%）, meanwhile,
There is adverse reaction in none example in observed case history.In addition, the eye drip liquid energy containing carnosine be obviously improved it is increased with the age
Hypopsia（Presbyopia）And mitigate visual fatigue.It is external that carnosine is used for food addition, tonic.
The conventional synthesis technique for preparing carnosine for-alanine and phthalic anhydride generation phthalyl--
Alanine, protects amino, carboxyl and thionyl chloride reaction generation phthalyl-- alanyl chloride, then with trim,ethylchlorosilane
The L-Histidine formation peptide bond of protection.This route yield is low, easily racemization during peptide bond is formed, and influences purity, and solvent consumption mistake
Height, easily causes environmental pollution, route is longer in addition, is unfavorable for industrialization.
The content of the invention
Utility model purpose：Above-mentioned in order to overcome the shortcomings of, the purpose of this utility model provides a kind of environmentally friendly, efficient, easy
A kind of process system for preparing carnosine of operation.
Technical scheme：A kind of process system for preparing carnosine, including the first reactor（1）, the second reactor（2）, first
Centrifuge（3）, ultrafiltration apparatus（5）, nanofiltration equipment（6）, crystallization tank（7）, the second centrifuge（8）；Also include methanol basin（9）、
Thionyl chloride basin（10）, Beta-alanine basin（11）, the methanol basin（9）, thionyl chloride basin（10）, Beta-alanine storage
Tank（11）Positioned at first reactor（1）Upstream, and respectively with first reactor（1）Pipeline is connected；Also include L- groups
Propylhomoserin basin（12）, deionized water basin（13）, alkaline solution basin（14）；The L-Histidine basin（12）, deionized water
Basin（13）, alkaline solution basin（14）Positioned at second reactor（2）Upstream, and respectively with second reactor（2）
Pipeline is connected；Also include Beta-alanine methyl ester hydrochloride basin（4）, the Beta-alanine methyl ester hydrochloride basin（4）Respectively with institute
State the first reactor（1）, the second reactor（2）Pipeline is connected.
Preferably, the ultrafiltration apparatus（5）Filter sizes be 8000-10000 dalton.
Preferably, the nanofiltration equipment（6）Filter sizes be 200-300 dalton.
A kind of method using the above-mentioned process system for preparing carnosine, comprises the following steps：
1）To the first reactor（1）Middle addition methanol 400-700g/L, 0-20 DEG C of temperature control is by thionyl chloride 200-300g/L
Instill wherein, completion of dropping adds Beta-alanine 150-200g/L, 30-60 DEG C of insulated and stirred 1-5h；
2）30-50 DEG C, vacuum≤- 0.09MPa is concentrated under reduced pressure, and is concentrated into flowing out without cut and goes out, and obtains Beta-alanine methyl esters salt
Hydrochlorate；
3）To the second reactor（2）Middle addition L-Histidine 20-40g/L, deionized water 400-800g/L, Beta-alanine first
Ester hydrochloride 25-50g/L；
4）At 15-45 DEG C, alkaline solution basin is used（14）Middle solution adjusts pH value to 7.0-9.0；
5）With alkaline basin in 25-45 DEG C of stirring reaction 2-4h, course of reaction（14）Middle solution controls pH value of reaction system
In 7.0-9.0；
6）Reaction is finished, blowing, and feed liquid is through the first centrifuge（3）Insoluble matter is removed, filtrate is collected；
7）Obtained filtrate is passed through into ultrafiltration apparatus（5）Ultrafiltration is carried out, ultrafiltration dialysis liquid is collected；
8）Obtained ultrafiltration dialysis liquid is passed through into nanofiltration equipment（6）Nanofiltration is carried out, nanofiltration concentrate is collected；
9）50-80 DEG C, vacuum≤- 0.09MPa is concentrated under reduced pressure into carnosine content 20-40%；
10）Add organic solvent, 0-25 DEG C, stirring, through crystallization tank（7）Crystallization 10-24h.
11）Crystallization is finished, and is discharged to the second centrifuge（8）, 30-50 DEG C, vacuum≤- 0.09MPa is dried under reduced pressure 6-
8h, obtains carnosine crude product.
Described alkaline solution is sodium hydroxide, sodium carbonate, sodium acid carbonate, potassium hydroxide, potassium carbonate, saleratus, ammonia
One kind in water.
Described organic solvent is one kind in methanol, ethanol, isopropanol or acetone.
Beneficial effect：The utility model removes macromolecular enzyme and small molecule inorganic salts using ultrafiltration, nanofiltration, pollute it is small,
Cost is low.The utility model carnosine reaction production rate is high, reaches more than 95%；Compared to conventional chemical methods, its pollution is small, reaction
Cycle is short, more conducively industrialization, and energy consumption is low.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
With reference to specific embodiment, the utility model is expanded on further.It should be understood that these embodiments are merely to illustrate this
Utility model rather than limitation scope of the present utility model.
Embodiment 1
Such as Fig. 1, a kind of process system for preparing carnosine, including the first reactor（1）, the second reactor（2）, first centrifugation
Machine（3）, ultrafiltration apparatus（5）, nanofiltration equipment（6）, crystallization tank（7）, the second centrifuge（8）；Also include methanol basin（9）, chlorination
Sulfoxide basin（10）, Beta-alanine basin（11）, the methanol basin（9）, thionyl chloride basin（10）, Beta-alanine basin
（11）Positioned at first reactor（1）Upstream, and respectively with first reactor（1）Pipeline is connected；Also include L- group ammonia
Sour basin（12）, deionized water basin（13）, alkaline solution basin（14）；The L-Histidine basin（12）, deionized water storage
Tank（13）, alkaline solution basin（14）Positioned at second reactor（2）Upstream, and respectively with second reactor（2）Pipe
Road is connected；Also include Beta-alanine methyl ester hydrochloride basin（4）, the Beta-alanine methyl ester hydrochloride basin（4）Respectively with it is described
First reactor（1）, the second reactor（2）Pipeline is connected.The ultrafiltration apparatus（5）Filter sizes be 8000-10000 dongles
.The nanofiltration equipment（6）Filter sizes be 200-300 dalton.
Claims (3)
1. a kind of process system for preparing carnosine, it is characterised in that：Including the first reactor（1）, the second reactor（2）, first
Centrifuge（3）, ultrafiltration apparatus（5）, nanofiltration equipment（6）, crystallization tank（7）, the second centrifuge（8）；Also include methanol basin（9）、
Thionyl chloride basin（10）, Beta-alanine basin（11）, the methanol basin（9）, thionyl chloride basin（10）, Beta-alanine storage
Tank（11）Positioned at first reactor（1）Upstream, and respectively with first reactor（1）Pipeline is connected；Also include L- groups
Propylhomoserin basin（12）, deionized water basin（13）, alkaline solution basin（14）；The L-Histidine basin（12）, deionized water
Basin（13）, alkaline solution basin（14）Positioned at second reactor（2）Upstream, and respectively with second reactor（2）
Pipeline is connected；Also include Beta-alanine methyl ester hydrochloride basin（4）, the Beta-alanine methyl ester hydrochloride basin（4）Respectively with institute
State the first reactor（1）, the second reactor（2）Pipeline is connected.
2. a kind of process system as claimed in claim 1 for preparing carnosine, it is characterised in that：The ultrafiltration apparatus（5）Filter
Membrane aperture is 8000-10000 dalton.
3. a kind of process system as claimed in claim 1 for preparing carnosine, it is characterised in that：The nanofiltration equipment（6）Filter
Membrane aperture is 200-300 dalton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720124700.0U CN206502762U (en) | 2017-02-12 | 2017-02-12 | Prepare the process system of carnosine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720124700.0U CN206502762U (en) | 2017-02-12 | 2017-02-12 | Prepare the process system of carnosine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206502762U true CN206502762U (en) | 2017-09-19 |
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ID=59834872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720124700.0U Active CN206502762U (en) | 2017-02-12 | 2017-02-12 | Prepare the process system of carnosine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206502762U (en) |
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2017
- 2017-02-12 CN CN201720124700.0U patent/CN206502762U/en active Active
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