CN104371910B - A kind of method extracting collagen from deer bone - Google Patents
A kind of method extracting collagen from deer bone Download PDFInfo
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- CN104371910B CN104371910B CN201410605513.5A CN201410605513A CN104371910B CN 104371910 B CN104371910 B CN 104371910B CN 201410605513 A CN201410605513 A CN 201410605513A CN 104371910 B CN104371910 B CN 104371910B
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 51
- 108010035532 Collagen Proteins 0.000 title claims abstract description 51
- 229920001436 collagen Polymers 0.000 title claims abstract description 51
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 43
- 241000282994 Cervidae Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 28
- 108090000790 Enzymes Proteins 0.000 claims abstract description 28
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 24
- 230000007062 hydrolysis Effects 0.000 claims abstract description 17
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 17
- 238000005554 pickling Methods 0.000 claims abstract description 13
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000000746 purification Methods 0.000 claims abstract description 9
- 230000008859 change Effects 0.000 claims abstract description 7
- 238000010828 elution Methods 0.000 claims abstract description 5
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 5
- 239000003456 ion exchange resin Substances 0.000 claims abstract description 5
- 229920003303 ion-exchange polymer Polymers 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 24
- 230000008569 process Effects 0.000 claims description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical group Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 8
- 238000005238 degreasing Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- 229910052791 calcium Inorganic materials 0.000 claims description 6
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 5
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 5
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 5
- 239000001099 ammonium carbonate Substances 0.000 claims description 5
- 238000002425 crystallisation Methods 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 2
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 claims description 2
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 claims description 2
- 229910001948 sodium oxide Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 239000002537 cosmetic Substances 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 6
- 239000003513 alkali Substances 0.000 abstract description 5
- 239000002932 luster Substances 0.000 abstract description 5
- 239000002253 acid Substances 0.000 abstract description 4
- 125000003277 amino group Chemical group 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 description 22
- 238000000605 extraction Methods 0.000 description 8
- 239000003957 anion exchange resin Substances 0.000 description 7
- 239000003729 cation exchange resin Substances 0.000 description 6
- 210000005056 cell body Anatomy 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000003292 glue Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 150000001450 anions Chemical class 0.000 description 4
- 239000003223 protective agent Substances 0.000 description 4
- 239000012266 salt solution Substances 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000000460 chlorine Substances 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- -1 biology Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241001550206 Colla Species 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- SYWDWCWQXBUCOP-UHFFFAOYSA-N benzene;ethene Chemical class C=C.C1=CC=CC=C1 SYWDWCWQXBUCOP-UHFFFAOYSA-N 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 210000002318 cardia Anatomy 0.000 description 1
- 235000017168 chlorine Nutrition 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C12M45/00—Means for pre-treatment of biological substances
- C12M45/06—Means for pre-treatment of biological substances by chemical means or hydrolysis
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of method extracting collagen from deer bone, including following setting gradually, front latter linked each device: one-level breaker, rinsing bowl, alkaline bath, elution device, pickling tank, two-stage crushing device, enzymatic vessel, groove of saltouing, enzyme hydrolysis tank, purification devices, purification devices includes the first ultrafiltration apparatus, the second ultrafiltration apparatus and ion exchange resin bed, the molecule interception of the first ultrafiltration apparatus is 3～50,000 dalton, and the molecule interception of the second ultrafiltration apparatus is 0.5～10,000 dalton.In said system, the temperature of whole processing procedure is not over 50 DEG C, and uses enzymolysis to extract, it is to avoid acid and alkali hydrolysis causes collagen sex change, the problem that amine groups molecular structure damages, and saves the triple-helix structure of natural activity.Simultaneously by using purifier apparatus, the collagen purity prepared, color and luster, taste, clarity, ash quality of grading is characterized, the molecular weight of the collagen obtained is between 1～40,000, when for medicine, cosmetic field, it is possible to play the pharmaceutically active of collagen completely.
Description
Technical field
The present invention relates to functional protein and extract field, be specifically related to a kind of extraction collagen egg from deer bone
White method.
Background technology
China is stag breeding big country, and pilose antler, venison are the target products of stag breeding, in addition,
Also can produce deer blood, deer tire, deer bone etc. and there is the accessory substance of higher medical value.Deer bone contains
The mineral such as substantial amounts of collagen, Phospholipids, phosphoprotein, vitamin and calcium, magnesium, iron, zinc
Element, has important physiological function and medical active.The Colla 0ssis Cervi that deer bone prepares has tonifying Qi benefit
Blood, the effect dispelled rheumatism, it is possible to effectively treatment marrow is not enough, spray the illness such as blood, rheumatism.Glue
Former albumen is the main composition of deer bone, and collagen has close with the aging of human body and resistance
Cutting link, is widely used in the fields such as food, health products, medicine, cosmetics.It addition,
Collagen hydrolysate is active peptides, it is possible to protection stomach lining, hypotensive, anti-oxidant, anti-aging.
Deer bone is the raw material that a kind of high-quality collagen extracts, and currently also occurs that some carry from deer bone
The method taking collagen, mainly has acid system and enzyme process, but, the glue that these methods are extracted at present
Former albumen is difficult to maintain its natural triple-helix structure, and in collagen, component is many, bigger molecule and
Micromolecular collagen accounting is big, is applied to the effect reduction of medicine, biology, cosmetic field.
Meanwhile, the color and luster of collagen for preparing, taste, clarity, molecular weight, ash character of grading is poor,
It addition, at the bottom of the recovery rate of whole collagen, cycle length, energy consumption high, required inorganic agent is used
Amount is big, produces finished product high.
Summary of the invention
It is an object of the invention to provide a kind of method extracting collagen from deer bone, it can have
Effect solves the problems referred to above, extraction collagen from deer bone easily and fast, and the collagen extracted
The purity of albumen is high, quality better.
For achieving the above object, the present invention uses following technical side to implement:
A kind of method extracting collagen from deer bone, including following setting gradually, front and back connecting
Each device:
Deer bone after removing marrow is carried out the one-level breaker of primary breakup；
One-level deer bone after broken is carried out successively rinsing bowl and the alkaline bath of degreasing；
Deer bone after degreasing is carried out the elution device of drip washing；
Deer bone after drip washing is carried out the pickling tank of decalcification；
Deer bone after removing calcium is carried out the two-stage crushing device of micronization processes；
Bone mud after micronization processes is carried out the enzymatic vessel of enzymolysis processing；
Enzymolysis liquid after enzymolysis is saltoutd and processes the groove of saltouing separating out thick collagen；
Thick collagen after saltouing is carried out the enzyme hydrolysis tank of enzyme hydrolysis process；
Enzyme hydrolyzate after enzyme hydrolysis is purified the purification devices of process, and purification devices includes depending on
Secondary setting:
Enzyme hydrolyzate carries out the first ultrafiltration apparatus of one-level ultrafiltration, and the molecule of the first ultrafiltration apparatus cuts
Allowance is 3～50,000 dalton；
Penetrating fluid after one-level ultrafiltration is carried out the second ultrafiltration apparatus of two-stage ultrafiltering, the second ultrafiltration dress
The molecule interception put is 0.5～10,000 dalton；
Concentrate after two-stage ultrafiltering is carried out deionized ion exchange resin bed.
In said system, the temperature of whole processing procedure is not over 50 DEG C, and uses enzymolysis to carry out
Extract, it is to avoid acid and alkali hydrolysis causes collagen sex change, and what amine groups molecular structure damaged asks
Topic, saves the triple-helix structure of natural activity.Simultaneously by using purifier apparatus so that prepare
Collagen purity, color and luster, taste, clarity, ash quality of grading characterize, the glue obtained
The molecular weight of former albumen is between 1～40,000, when for medicine, cosmetic field, it is possible to complete
The full pharmaceutically active playing collagen.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Detailed description of the invention
In order to make objects and advantages of the present invention clearer, below in conjunction with embodiment to this
Bright it is specifically described.Should be appreciated that following word is only in order to describe the one of the present invention or several
Planting specific embodiment, the protection domain of not concrete to present invention request carries out considered critical.
Present invention aim at providing a kind of method extracting collagen from deer bone, such as Fig. 1 institute
Show, including following setting gradually, front latter linked each device:
Deer bone after removing marrow carries out the one-level breaker 11 of primary breakup, and deer bone has picked
Unless the material of bone parts；
One-level deer bone after broken is carried out successively rinsing bowl 12 and the alkaline bath 13 of degreasing；
Deer bone after degreasing carries out the elution device 14 of drip washing, and elution device 14 includes a conveying
Band, the inclined layout of conveyor surface of conveyer belt, the discharging opening phase of conveyer belt low side and alkaline bath 13
Corresponding layout, the corresponding layout of charging aperture of the high-end and pickling tank of conveyer belt, the zone face of conveyer belt
Constituting for guipure, it is clear for spraying on conveyer belt that being provided above of conveyer belt arranges that shape arranges
Water carries out the shower of drip washing to deer bone on conveyer belt；
Deer bone after drip washing carries out the pickling tank 15 of decalcification, and pickling tank 15 includes cell body, cell body
Inside being provided with the screen frame of a splendid attire deer bone, screen frame is hung on a rocker, rocker and drive mechanism phase
Connecting, drive mechanism is ordered about pendulum frame and is swung, and the bottom land of cell body is bucket-shaped, and bottom land lowest part sets
Put discharge port；
Deer bone after removing calcium is carried out the two-stage crushing device 16 of micronization processes；
Bone mud after micronization processes is carried out the enzymatic vessel 17 of enzymolysis processing；
Enzymolysis liquid after enzymolysis is saltoutd and processes the groove 18 of saltouing separating out thick collagen, saltout
In groove 18, the content of sodium chloride is 4%；
Thick collagen after saltouing is carried out the enzyme hydrolysis tank 19 of enzyme hydrolysis process；
Enzyme hydrolyzate after enzyme hydrolysis is purified the purification devices of process, and purification devices includes depending on
Secondary setting:
Enzyme hydrolyzate is carried out the first ultrafiltration apparatus 20 of one-level ultrafiltration, the first ultrafiltration apparatus 20
Molecule interception is 3～50,000 dalton；
Penetrating fluid after one-level ultrafiltration is carried out the second ultrafiltration apparatus 21 of two-stage ultrafiltering, the second ultrafiltration
The molecule interception of device 21 is 0.5～10,000 dalton；
Concentrate after two-stage ultrafiltering is carried out deionized ion exchange resin bed 22.
One-level breaker 11 is for rolling breaker, and two-stage crushing device 16 is that roller grinds powder dress
Put.This system also includes reclaiming the anion and cation exchange resin on ion exchange resin bed
Anion and cation exchange resin regenerated reactor 23,24, anion and cation exchange resin regenerated reactor 23,
The leakage fluid dram of 24 and the dog-house of the first reaction tank are connected.
Between above-mentioned neighboring devices, solid material uses conveyer belt to carry, and liquid material uses pipe
Road delivery pump carries, and needs the employing filter carrying out separation of solid and liquid to operate.This
In the said system of bright offer, use collagen in enzymolysis-water enzymolysis and extraction deer bone, it is to avoid pass
The problem that system acidity extraction is easily caused collagen sex change so that the collagen of extraction can be protected
Hold natural triple-helix structure, meanwhile, by the associating of enzymolysis-water enzymolysis so that the glue of extraction
Former molecular weight of albumen can concentrate between 2～40,000, it is to avoid excessive and mistake small-molecular-weight after extraction
Collagen occupancy volume excessive.It addition, chosen by the purifying of follow-up purification devices so that
Prepare collagen product molecular weight between 1～50,000, it is ensured that collagen for medicine,
Optimal medical active can have been given play to during cosmetics.Meanwhile, prepare collagen purity, color and luster,
Clarity, ash quality of grading characterizes, and the recovery rate of collagen has also reached 69.11%.
During concrete operations, the tank liquor in alkaline bath 13 is NaOH, tank liquor in alkaline bath 13
PH maintains 11～11.5.Tank liquor in pickling tank 15 is hydrochloric acid solution, pickling tank 15 inside groove
The PH of liquid maintains 2～2.5.The regulation and control of above-mentioned acid-base value, mainly prevent carry out degreasing and
During decalcification, the impact on collagen activity of the soda acid treatment fluid, the water temperature in rinsing bowl 12 is 40
About DEG C.Tank liquor in alkaline bath 13 and pickling tank 15 is in running water state, and cell body is provided with
Arranging valve at the feed liquor mouth of pipe and drain pipe mouth and the mouth of pipe, the PH detector in cell body is according to detection
PH carry out the open/close states of control valve, thus the PH of tank liquor in regulating and controlling cell body.
Owing to using hydrochloric acid that deer bone is carried out decalcification, after so long-term decalcification, substantial amounts of chlorine can be produced
Changing calcium and separate out precipitation in the bottom of pickling tank 15, these sediments are impregnated with substantial amounts of after being separated
Hydrochloric acid, also can produce in anion and cation exchange resin regenerated reactor 23,24 simultaneously substantial amounts of acid,
Alkali wasteliquid, these dreg liquids directly discharge and pollute the environment.Therefore the further scheme of the present invention is,
This system also includes the dreg liquid processing means processing the dreg liquid after decalcification, at dreg liquid
Reason device includes carrying out, for dreg liquid and sodium carbonate, the first reaction tank 31 of reacting, anti-for first
The sodium chloride solution obtained in answering pond 31 and ammonium hydrogen carbonate carry out the second reaction tank 32 reacted, and use
In the second reaction tank 32, pond liquid carries out the crystallizing pond 35 of crystallisation by cooling, in crystallizing pond 35
Crystalline solid and soda bath carry out the 3rd reaction tank 33 reacted, and for the 3rd reaction tank 33
The sodium carbonate liquor inside obtained and aqua calcis carry out reacting the 4th anti-of preparing hydrogen sodium oxide molybdena
Answer pond 34.The liquid outlet of the 4th reaction tank 34 is connected with the inlet of alkaline bath 13.4th is anti-
The liquid outlet in pond 34 is answered to be connected with the inlet of groove 18 of saltouing.By dreg liquid at the first reaction tank
In 31, the sodium carbonate with excess reacts, and changes into sodium chloride and precipitation of calcium carbonate, prepared salt solution one
Part may be used for follow-up collagen and saltouts process；The most unnecessary salt solution is at the second reaction tank
In react with ammonium hydrogen carbonate, crystallization recovery ammonium hydrogen carbonate and ammonium chloride, chlorine after cooling
Change ammonium to use as the fertilizer of foster deer herbage.And the ammonium hydrogen carbonate reclaimed is at third and fourth
Reaction tank reacts with NaOH, calcium hydroxide, finally obtains sodium hydroxide solution,
The sodium hydroxide solution the arrived ungrease treatment for deer bone and the regeneration of anion exchange resin,
To calcium carbonate also be used as his use, thus realize dreg liquid clean process.
The scheme being more highly preferred to is: leakage fluid dram and first filter of the first reaction tank are connected,
The leakage fluid dram of the first filter is connected with concentration kettle, the concentrate outlet of concentration kettle and second
Reaction tank is connected, and the concentrate outlet of concentration kettle is also attached with secondary concentration still, two grades
The liquid outlet of concentration kettle is connected with crystallization tank, and crystallization tank and the second filter are connected, and second
The solids outlet port of filter is connected with the dissolving tank dissolving crystalline solid, dissolving tank
Leakage fluid dram is connected with groove of saltouing, and the liquid outlet of the second filter and the second reaction tank are connected
Connect, between pickling tank and the first reaction tank, be provided with the 3rd filter.Concentrated still and two grades dense
After contracting still concentrates, the concentration of salt solution is substantially saturated, and now crystallizes, and obtains the chlorine that purity is the highest
Change sodium, be dissolved in water and be configured to salt solution saltouing for collagen, it is to avoid impurity when saltouing
Introduce, improve the purity of the deer-bone collagenous albumen of finished product.
More specifically scheme is: in enzymatic vessel 17, the enzyme used by enzymolysis processing is pepsin, enzyme
The time that solution processes is 10～12h, PH are 1.5～2.0, and temperature 30～35 DEG C, stomach cardia is pressed
It is added according in bone mud the 1.0～1.5% of collagen content.Enzyme hydrolysis in enzyme hydrolysis tank 19
Enzyme used by process is alkali protease, and the time of enzymolysis processing is 3～4h, PH are 9.5～10.5,
Temperature 35～40 DEG C, alkali protease is added according to the 2.0～2.5% of thick collagen, enzyme
Complex enzyme hydrolysis protective agent it is also added in hydrolytic decomposition pot 19.Complex enzyme hydrolysis protective agent enables to alkalescence
Hydrolase does not destroy the pharmaceutically active of hydrolysate while being oriented enzymolysis processing, it is ensured that glue
The homogeneity of former molecular weight of albumen, it is ensured that the pharmaceutically active of collagen and quality after enzyme hydrolysis.Multiple
Synthase solution protective agent can be selected for YY10611-6-complex enzyme protective agent AFP or M0111-9 complex enzyme is protected
Protect agent MP.
First and second ultrafiltration apparatus is tubular ultra-filtration membrane device, the penetrating fluid of I and II hyperfiltration treatment
Flow is respectively 50～60L/m2.h, milipore filter includes poly-inclined tetrafluoroethene, polyether sulfone.Ion is handed over
Change and on resin bed 22, be provided with cationic ion-exchange resin and anion exchange resin, such as strong basicity benzene
Ethene series anion exchange resin, strongly acidic styrene type cation exchange resin.
In a word, the method extracting collagen from deer bone that the present invention provides, it can facilitate, soon
The extraction collagen from deer bone of speed, and the purity of the collagen extracted is high, purity is up to 96%
Above, by electrophoresis molecular assay, the molecular weight of collagen concentrates between 2～3.8 ten thousand,
And the natural activity of collagen has been fully retained, it is possible to it is effective to medicine and cosmetics, it addition,
The qualities such as color and luster, taste, clarity characterize also excellent.
The above is only the preferred embodiment of the present invention, it is noted that for the art
Those of ordinary skill for, after knowing content described in the present invention, former without departing from the present invention
On the premise of reason, it is also possible to it is made some equal conversion and replacement, these convert on an equal basis and replace
In generation, also should be regarded as belonging to protection scope of the present invention.
Claims (1)
1. the method extracting collagen from deer bone, sets gradually, front and back including following
The each device connected:
Deer bone after removing marrow is carried out the one-level breaker of primary breakup；
One-level deer bone after broken is carried out successively rinsing bowl and the alkaline bath of degreasing；
Deer bone after degreasing is carried out the elution device of drip washing；
Deer bone after drip washing is carried out the pickling tank of decalcification；
Deer bone after removing calcium is carried out the two-stage crushing device of micronization processes；
Bone mud after micronization processes is carried out the enzymatic vessel of enzymolysis processing；
Enzymolysis liquid after enzymolysis is saltoutd and processes the groove of saltouing separating out thick collagen；
Thick collagen after saltouing is carried out the enzyme hydrolysis tank of enzyme hydrolysis process；
Enzyme hydrolyzate after enzyme hydrolysis is purified the purification devices of process, and purification devices includes successively
Arrange:
Enzyme hydrolyzate carries out the first ultrafiltration apparatus of one-level ultrafiltration, and the molecule of the first ultrafiltration apparatus cuts
Allowance is 3～50,000 dalton；
Penetrating fluid after one-level ultrafiltration is carried out the second ultrafiltration apparatus of two-stage ultrafiltering, the second ultrafiltration dress
The molecule interception put is 0.5～10,000 dalton；
Concentrate after two-stage ultrafiltering is carried out deionized ion exchange resin bed；
Tank liquor in alkaline bath is NaOH, and in alkaline bath, the PH of tank liquor maintains 11～11.5；
Tank liquor in pickling tank is hydrochloric acid solution, and in pickling tank, the PH of tank liquor maintains 2～2.5；
Also including the dreg liquid processing means processing the dreg liquid after decalcification, dreg liquid processes
Device includes carrying out the first reaction tank of reacting, for the first reaction tank for dreg liquid and sodium carbonate
The sodium chloride solution inside obtained and ammonium hydrogen carbonate carry out the second reaction tank reacted, for the second reaction
In pond, pond liquid carries out the crystallizing pond of crystallisation by cooling, and the crystalline solid in crystallizing pond enters with soda bath
3rd reaction tank of row reaction, and the sodium carbonate liquor that obtains in the 3rd reaction tank and hydrogen-oxygen
Change calcium solution and carry out reacting the 4th reaction tank of preparing hydrogen sodium oxide molybdena；The liquid outlet of the 4th reaction tank with
The inlet of alkaline bath is connected；The liquid outlet of the first reaction tank is connected with the inlet of groove of saltouing
Connect.
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| CN201610016664.6A CN105504048B (en) | 2014-10-31 | 2014-10-31 | Method for extracting collagen from deer bone |
| CN201610016667.XA CN105505769B (en) | 2014-10-31 | 2014-10-31 | A kind of technique for extracting collagen from deer bone |
| CN201410605513.5A CN104371910B (en) | 2014-10-31 | 2014-10-31 | A kind of method extracting collagen from deer bone |
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| CN108179166A (en) * | 2018-03-28 | 2018-06-19 | 通化百泉保健食品有限公司 | A kind of industrialized producing technology of deer whip albumen oligopeptide |
| CN111235202B (en) * | 2018-11-29 | 2022-03-08 | 中国中医科学院医学实验中心 | Deer bone protein extract and preparation method and application thereof |
| CN111363030A (en) * | 2020-03-20 | 2020-07-03 | 青岛小度信息科技有限公司 | Collagen peptide production and purification system |
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| CN105505769B (en) | 2018-12-07 |
| CN105504048B (en) | 2022-01-28 |
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