CN204211727U - A kind of system extracting collagen protein from deer bone - Google Patents
A kind of system extracting collagen protein from deer bone Download PDFInfo
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- CN204211727U CN204211727U CN201420646512.0U CN201420646512U CN204211727U CN 204211727 U CN204211727 U CN 204211727U CN 201420646512 U CN201420646512 U CN 201420646512U CN 204211727 U CN204211727 U CN 204211727U
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Abstract
The utility model discloses a kind of system extracting collagen protein from deer bone, comprise followingly to set gradually, front latter linked each device: one-level shredder assembly, washing bath, alkaline bath, elution device, pickling tank, two-stage crushing device, enzymatic vessel, groove of saltouing, enzymic hydrolysis tank, purification devices, purification devices comprises the first ultra-filtration equipment, the second ultra-filtration equipment and ion exchange resin bed, the molecular retention amount of the first ultra-filtration equipment is 3 ~ 50,000 dalton, and the molecular retention amount of the second ultra-filtration equipment is 0.5 ~ 10,000 dalton.In said system, the temperature of whole treating processes will more than 50 DEG C, and adopt enzymolysis to extract, and avoids acid and alkali hydrolysis to cause denature collagen, the problem that amine groups molecular structure damages, saves the triple-helix structure of natural radioactivity.
Description
Technical field
The utility model relates to functional protein and extracts field, is specifically related to a kind of system extracting collagen protein from deer bone.
Background technology
China is stag breeding big country, and pilose antler, venison are the target products of stag breeding, in addition, also can produce the by product that deer blood, deer tire, deer bone etc. have higher pharmaceutical use.Containing a large amount of mineral elements such as collagen protein, phospholipid, phosphorprotein, VITAMIN and calcium, magnesium, iron, zinc in deer bone, there is important physiological function and medical active.Effect that the Colla 0ssis Cervi that deer bone obtains has invigorating qi and benefiting blood, dispels rheumatism, effectively can treat marrow deficiency, illness such as spray blood, rheumatism etc.Collagen protein is the main composition of deer bone, and aging and the resistibility of collagen protein and human body have close ties, are widely used in the fields such as food, healthcare products, medicine, makeup.In addition, collagen hydrolysate is active polypeptide, can protect gastric mucosa, hypotensive, anti-oxidant, anti-aging.
Deer bone is the raw material that a kind of high-quality collagen protein extracts, also occur that some extract the system of collagen protein from deer bone at present, mainly contain acid system and enzyme process, but, the collagen protein that these methods are extracted at present is difficult to maintain its natural triple-helix structure, in collagen protein, component is many, large compared with macromole and micromolecular collagen accounting, be applied to medicine, biology, cosmetic field effect reduce.Meanwhile, the color and luster of obtained collagen protein, taste, clarity, molecular weight, ash grade character difference, and in addition, at the bottom of the extraction yield of whole collagen protein, the cycle is long, energy consumption is high, and required treatment agent consumption is large, and manufacture a finished product height.
Utility model content
The purpose of this utility model is to provide a kind of system extracting collagen protein from deer bone, and it can effectively solve the problem, easily and fast from deer bone, extract collagen protein, and the purity of collagen protein extracted is high, quality better.
For achieving the above object, the utility model adopts following technical side to implement:
From deer bone, extract a system for collagen protein, comprise followingly to set gradually, front latter linked each device:
Deer bone after removal marrow is carried out to the one-level shredder assembly of primary breakup;
Deer bone after one-level fragmentation is carried out successively to washing bath and the alkaline bath of degreasing;
Deer bone after degreasing is carried out to the elution device of drip washing;
Deer bone after drip washing is carried out to the pickling tank of decalcification;
To the two-stage crushing device going the deer bone after calcium to carry out thinning processing;
Bone mud after thinning processing is carried out to the enzymatic vessel of enzymolysis processing;
Enzymolysis solution after enzymolysis is saltoutd and processes the groove of saltouing of the thick collagen protein of precipitation;
Thick collagen protein after saltouing is carried out to the enzymic hydrolysis tank of enzymic hydrolysis process;
Enzyme hydrolyzate after enzymic hydrolysis is carried out to the purification devices of purification process, purification devices comprises and setting gradually:
Enzyme hydrolyzate is carried out to the first ultra-filtration equipment of one-level ultrafiltration, the molecular retention amount of the first ultra-filtration equipment is 3 ~ 50,000 dalton;
Penetrating fluid after one-level ultrafiltration is carried out to the second ultra-filtration equipment of two-stage ultrafiltering, the molecular retention amount of the second ultra-filtration equipment is 0.5 ~ 10,000 dalton;
Deionized ion exchange resin bed is carried out to the concentrated solution after two-stage ultrafiltering;
This system also comprises the waste residue liquid treatment unit that the waste residue liquid after to decalcification processes, waste residue liquid treatment unit comprises and carries out for waste residue liquid and sodium carbonate the first reaction tank of reacting, the second reaction tank reacted is carried out for the sodium chloride solution that obtains in the first reaction tank and bicarbonate of ammonia, the crystallizing pond of crystallisation by cooling is carried out for pond liquid in the second reaction tank, the 3rd reaction tank reacted is carried out for the xln in crystallizing pond and caustic soda soln, and carry out reacting the 4th reaction tank of preparing hydrogen sodium oxide for the sodium carbonate solution that obtains in the 3rd reaction tank and aqua calcis.
In said system, the temperature of whole treating processes will more than 50 DEG C, and adopt enzymolysis to extract, and avoids acid and alkali hydrolysis to cause denature collagen, the problem that amine groups molecular structure damages, saves the triple-helix structure of natural radioactivity.Simultaneously by adopting purifier apparatus, the collagen protein purity making to obtain, color and luster, taste, clarity, ash quality of grading characterizes, the molecular weight of the collagen protein obtained, between 1 ~ 40,000, when for medicine, cosmetic field, can play the pharmaceutical activity of collagen protein completely.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Embodiment
In order to make the purpose of this utility model and advantage clearly understand, below in conjunction with embodiment, the utility model is specifically described.Should be appreciated that following word only in order to describe one or more concrete embodiments of the present utility model, considered critical is not carried out to the protection domain that the utility model is specifically asked.
The utility model object is to provide a kind of system extracting collagen protein from deer bone, as shown in Figure 1, comprise followingly to set gradually, front latter linked each device:
Deer bone after removal marrow is carried out to the one-level shredder assembly 11 of primary breakup, deer bone has rejected the material of non-bone parts;
Deer bone after one-level fragmentation is carried out successively to washing bath 12 and the alkaline bath 13 of degreasing;
Deer bone after degreasing is carried out to the elution device 14 of drip washing, elution device 14 comprises a conveying belt, the conveyor surface of conveying belt is in being in tilted layout, conveying belt low side layout corresponding with the discharge port of alkaline bath 13, the corresponding layout of opening for feed of the high-end and pickling tank of conveying belt, the zone face of conveying belt is that guipure is formed, and what the top of conveying belt was provided with that arrangement shape arranges carries out the shower of drip washing for spraying clear water on conveying belt to deer bone on conveying belt;
Deer bone after drip washing is carried out to the pickling tank 15 of decalcification, pickling tank 15 comprises cell body, the screen frame of a splendid attire deer bone is provided with in cell body, screen frame hanging is on a rocker, rocker is connected with driving mechanism, driving mechanism orders about pendulum frame and swings, and the bottom land of cell body is bucket-shaped, and bottom land lowest part arranges discharge opening;
To the two-stage crushing device 16 going the deer bone after calcium to carry out thinning processing;
Bone mud after thinning processing is carried out to the enzymatic vessel 17 of enzymolysis processing;
Saltout to the enzymolysis solution after enzymolysis and process the groove 18 of saltouing of the thick collagen protein of precipitation, in groove 18 of saltouing, the content of sodium-chlor is 4%;
Thick collagen protein after saltouing is carried out to the enzymic hydrolysis tank 19 of enzymic hydrolysis process;
Enzyme hydrolyzate after enzymic hydrolysis is carried out to the purification devices of purification process, purification devices comprises and setting gradually:
The molecular retention amount of enzyme hydrolyzate being carried out to the first ultra-filtration equipment 20, first ultra-filtration equipment 20 of one-level ultrafiltration is 3 ~ 50,000 dalton;
The molecular retention amount of the penetrating fluid after one-level ultrafiltration being carried out to the second ultra-filtration equipment 21, second ultra-filtration equipment 21 of two-stage ultrafiltering is 0.5 ~ 10,000 dalton;
Deionized ion exchange resin bed 22 is carried out to the concentrated solution after two-stage ultrafiltering.
One-level shredder assembly 11 is for rolling shredder assembly, and two-stage crushing device 16 is pair roller stone roller powder device.This system also comprises the anion and cation exchange resin regenerated reactor 23,24 reclaimed the anion and cation exchange resin on ion exchange resin bed, and the leakage fluid dram of anion and cation exchange resin regenerated reactor 23,24 is connected with the dog-house of the first reaction tank.
Between above-mentioned neighboring devices, solid materials adopts conveying belt to carry, and liquid material adopts pipeline delivery pump to carry, and needs the employing filtration unit carrying out solid-liquid separation to operate.In the said system that the utility model provides, adopt collagen protein in enzymolysis-water enzymolysis and extraction deer bone, traditional acidity extraction is avoided easily to cause the problem of denature collagen, make the collagen protein extracted can keep natural triple-helix structure, simultaneously, by the associating of enzymolysis-water enzymolysis, the collagen molecules amount extracted can being concentrated between 2 ~ 40,000, avoiding extracting the rear excessive collagen protein occupancy volume with crossing small-molecular-weight excessive.In addition, chosen by the purifying of follow-up purification devices, make the molecular weight of obtained collagen product between 1 ~ 50,000, when ensureing that collagen protein is used for medicine, makeup, best medical active can have been given play to.Meanwhile, obtained collagen protein purity, color and luster, clarity, ash quality of grading characterizes, and the extraction yield of collagen protein also reaches 69.11%.
During concrete operations, the tank liquor in alkaline bath 13 is sodium hydroxide, and in alkaline bath 13, the PH of tank liquor maintains 11 ~ 11.5.Tank liquor in pickling tank 15 is hydrochloric acid soln, and in pickling tank 15, the PH of tank liquor maintains 2 ~ 2.5.The regulation and control of above-mentioned potential of hydrogen, mainly prevent when carrying out degreasing and decalcification, and acid-alkali treatment liquid is on the impact of collagen protein activity, and the water temperature in washing bath 12 is about 40 DEG C.Tank liquor in alkaline bath 13 and pickling tank 15 is in running water state, cell body is provided with the feed liquor mouth of pipe and the fluid mouth of pipe and mouth of pipe place arranges valve, and the PH detector in cell body carrys out the open/close states of regulated valve according to the PH detected, thus the PH of tank liquor in regulation and control cell body.
Owing to adopting hydrochloric acid, decalcification is carried out to deer bone, after so long-term decalcification, a large amount of calcium chloride can be produced and separate out precipitation in the bottom of pickling tank 15, a large amount of hydrochloric acid is impregnated with after these throw outs are separated, also can produce a large amount of acid, alkali wasteliquid in anion and cation exchange resin regenerated reactor 23,24, these waste residue liquids directly discharge and pollute the environment simultaneously.Therefore further program of the utility model is, this system also comprises the waste residue liquid treatment unit that the waste residue liquid after to decalcification processes, waste residue liquid treatment unit comprises and carries out for waste residue liquid and sodium carbonate the first reaction tank 31 of reacting, the second reaction tank 32 reacted is carried out for the sodium chloride solution that obtains in the first reaction tank 31 and bicarbonate of ammonia, the crystallizing pond 35 of crystallisation by cooling is carried out for pond liquid in the second reaction tank 32, the 3rd reaction tank 33 reacted is carried out for the xln in crystallizing pond 35 and caustic soda soln, and carry out reacting the 4th reaction tank 34 of preparing hydrogen sodium oxide for the sodium carbonate solution that obtains in the 3rd reaction tank 33 and aqua calcis.The liquid outlet of the 4th reaction tank 34 is connected with the fluid inlet of alkaline bath 13.The liquid outlet of the 4th reaction tank 34 is connected with the fluid inlet of groove 18 of saltouing.React with excessive sodium carbonate in the first reaction tank 31 by waste residue liquid, change into sodium-chlor and precipitation of calcium carbonate, obtained salt solution is a part of be may be used for follow-up collagen protein and to saltout process; Salt solution unnecessary in addition reacts with bicarbonate of ammonia in the second reaction tank, and cooling post crystallization is separated out and reclaimed bicarbonate of ammonia and ammonium chloride, and ammonium chloride can use as the fertilizer of foster deer herbage.And the bicarbonate of ammonia reclaimed reacts with sodium hydroxide, calcium hydroxide in third and fourth reaction tank, finally obtain sodium hydroxide solution, the skimming treatment of the sodium hydroxide solution obtained for deer bone and the regeneration of anionite-exchange resin, the calcium carbonate obtained also can be used as his use, thus realize waste residue liquid clean process.
More preferred scheme is: the leakage fluid dram of the first reaction tank is connected with the first filtration unit, the leakage fluid dram of the first filtration unit is connected with concentration kettle, the concentrated solution relief outlet of concentration kettle is connected with the second reaction tank, the concentrated solution relief outlet of concentration kettle is also connected with secondary concentration still, the liquid outlet of secondary concentration still is connected with crystallizer tank, crystallizer tank is connected with the second filtration unit, the solids outlet port of the second filtration unit is connected with the dissolving tank dissolved xln, the leakage fluid dram of dissolving tank is connected with groove of saltouing, the liquid outlet of the second filtration unit is connected with the second reaction tank, the 3rd filtration unit is provided with between pickling tank and the first reaction tank.After concentration kettle and secondary concentration still concentrate, the concentration of salt solution is substantially saturated, now carries out crystallization, obtain the sodium-chlor that purity is very high, its water-soluble salt solution that is configured to is used for saltouing of collagen protein, the introducing of impurity when avoiding saltouing, improves the purity of the deer-bone collagenous albumen of finished product.
More specifically scheme is: the enzyme that in enzymatic vessel 17, enzymolysis processing is used is stomach en-, and the time of enzymolysis processing is 10 ~ 12h, PH is 1.5 ~ 2.0, temperature 30 ~ 35 DEG C, and Pepsin adds according to 1.0 ~ 1.5% of collagen content in bone mud.Enzymic hydrolysis tank 19 endoenzyme hydrolysis treatment enzyme used is Sumizyme MP; the time of enzymolysis processing is 3 ~ 4h, PH is 9.5 ~ 10.5; temperature 35 ~ 40 DEG C, Sumizyme MP adds according to 2.0 ~ 2.5% of thick collagen protein, also adds complex enzyme hydrolysis protective material in enzymic hydrolysis tank 19.Complex enzyme hydrolysis protective material can make alkaline hydrolysis enzyme while carrying out directional enzymatic process, not destroy the pharmaceutical activity of hydrolysate, ensures the homogeneity of collagen molecules amount, ensures pharmaceutical activity and the quality of collagen protein after enzymic hydrolysis.Complex enzyme hydrolysis protective material can select YY10611-6-prozyme protective material AFP or M0111-9 prozyme protective material MP.
First and second ultra-filtration equipment is tubular ultra-filtration membrane device, and the permeate flow of I and II uf processing is respectively 50 ~ 60L/m
2.h, ultra-filtration membrane comprises poly-inclined tetrafluoroethylene, polyethersulfone.Ion exchange resin bed 22 is provided with Zeo-karb and anionite-exchange resin, as strong-basicity styrene series anion exchange resin, strongly acidic styrene type cation exchange resin.
In a word, the system extracting collagen protein from deer bone that the utility model provides, it can extract collagen protein easily and fast from deer bone, and the purity of the collagen protein extracted is high, and purity can reach more than 96%, by electrophoresis molecular assay, the molecular weight of collagen protein concentrates between 2 ~ 3.8 ten thousand, and remains the natural radioactivity of collagen protein completely, can be effective to medicine and makeup, in addition, the quality such as color and luster, taste, clarity characterizes also excellent.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; to know in the utility model after contents; under the prerequisite not departing from the utility model principle; can also make some equal conversion to it and substitute, these convert on an equal basis and substitute and also should be considered as belonging to protection domain of the present utility model.
Claims (3)
1. from deer bone, extract a system for collagen protein, comprise followingly to set gradually, front latter linked each device:
Deer bone after removal marrow is carried out to the one-level shredder assembly of primary breakup;
Deer bone after one-level fragmentation is carried out successively to washing bath and the alkaline bath of degreasing;
Deer bone after degreasing is carried out to the elution device of drip washing;
Deer bone after drip washing is carried out to the pickling tank of decalcification;
To the two-stage crushing device going the deer bone after calcium to carry out thinning processing;
Bone mud after thinning processing is carried out to the enzymatic vessel of enzymolysis processing;
Enzymolysis solution after enzymolysis is saltoutd and processes the groove of saltouing of the thick collagen protein of precipitation;
Thick collagen protein after saltouing is carried out to the enzymic hydrolysis tank of enzymic hydrolysis process;
Enzyme hydrolyzate after enzymic hydrolysis is carried out to the purification devices of purification process, purification devices comprises and setting gradually:
Enzyme hydrolyzate is carried out to the first ultra-filtration equipment of one-level ultrafiltration, the molecular retention amount of the first ultra-filtration equipment is 3 ~ 50,000 dalton;
Penetrating fluid after one-level ultrafiltration is carried out to the second ultra-filtration equipment of two-stage ultrafiltering, the molecular retention amount of the second ultra-filtration equipment is 0.5 ~ 10,000 dalton;
Deionized ion exchange resin bed is carried out to the concentrated solution after two-stage ultrafiltering;
This system also comprises the waste residue liquid treatment unit that the waste residue liquid after to decalcification processes, waste residue liquid treatment unit comprises and carries out for waste residue liquid and sodium carbonate the first reaction tank of reacting, the second reaction tank reacted is carried out for the sodium chloride solution that obtains in the first reaction tank and bicarbonate of ammonia, the crystallizing pond of crystallisation by cooling is carried out for pond liquid in the second reaction tank, the 3rd reaction tank reacted is carried out for the xln in crystallizing pond and caustic soda soln, and carry out reacting the 4th reaction tank of preparing hydrogen sodium oxide for the sodium carbonate solution that obtains in the 3rd reaction tank and aqua calcis.
2. the system extracting collagen protein from deer bone according to claim 1, is characterized in that: the liquid outlet of the 4th reaction tank is connected with the fluid inlet of alkaline bath; The liquid outlet of the 4th reaction tank is connected with the fluid inlet of groove of saltouing.
3. the system extracting collagen protein from deer bone according to claim 2, it is characterized in that: the leakage fluid dram of the first reaction tank is connected with the first filtration unit, the leakage fluid dram of the first filtration unit is connected with concentration kettle, the concentrated solution relief outlet of concentration kettle is connected with the second reaction tank, the concentrated solution relief outlet of concentration kettle is also connected with secondary concentration still, the liquid outlet of secondary concentration still is connected with crystallizer tank, crystallizer tank is connected with the second filtration unit, the solids outlet port of the second filtration unit is connected with the dissolving tank dissolved xln, the leakage fluid dram of dissolving tank is connected with groove of saltouing, the liquid outlet of the second filtration unit is connected with the second reaction tank, the 3rd filtration unit is provided with between pickling tank and the first reaction tank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420646512.0U CN204211727U (en) | 2014-10-31 | 2014-10-31 | A kind of system extracting collagen protein from deer bone |
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| CN201420646512.0U CN204211727U (en) | 2014-10-31 | 2014-10-31 | A kind of system extracting collagen protein from deer bone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646711A (en) * | 2020-10-29 | 2021-04-13 | 山东奥精生物科技有限公司 | High-purity collagen preparation automation line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646711A (en) * | 2020-10-29 | 2021-04-13 | 山东奥精生物科技有限公司 | High-purity collagen preparation automation line |
| CN112646711B (en) * | 2020-10-29 | 2023-02-17 | 山东奥精生物科技有限公司 | High-purity collagen preparation automation line |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20151031 |
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| EXPY | Termination of patent right or utility model |