CN108913726A - A kind of technique that biochemical method prepares acrylamide - Google Patents
A kind of technique that biochemical method prepares acrylamide Download PDFInfo
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- CN108913726A CN108913726A CN201810731872.3A CN201810731872A CN108913726A CN 108913726 A CN108913726 A CN 108913726A CN 201810731872 A CN201810731872 A CN 201810731872A CN 108913726 A CN108913726 A CN 108913726A
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- acrylamide
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
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Abstract
The invention discloses the techniques that a kind of biochemical method prepares acrylamide, using acrylonitrile and water as raw material, hydration reaction are carried out under the action of biocatalyst, end of reaction is filtered, refines, crystallizing and drying process is prepared.It is an advantage of the invention that：Using biocatalyst, environmental pollution is small, and recoverable, reduces use cost；Fully reacting, no coupling product, reaction condition is mild, low energy consumption, the three wastes are few.
Description
Technical field
The present invention relates to the production technology fields of acrylamide, and in particular to a kind of biochemical method prepares the work of acrylamide
Skill.
Background technique
Acrylamide is a kind of white crystal chemical substance, is the raw material for producing polyacrylamide.Polyacrylamide is main
For the purified treatment of water, the processing of paper pulp and inner coating of pipeline etc..The preparation of acrylamide commonly sulfuric acid water at present
Legal and catalytic hydration, sulfuric acid hydration process use the acrylonitrile and water of equimolar ratio, are hydrated under conditions of having sulfuric acid,
This method production acrylamide is intermittently operated, and process is complicated, and the acid, the alkali that consume in the process can corrode production equipment,
Lead to that the production cost increases, catalytic hydration commonly copper catalytic hydration, addition reaction can be generated during the reaction by depositing
And the drawbacks of generating a small amount of addition reaction.
Summary of the invention
In order to solve the above technical problems, the present invention provides the technique that a kind of biochemical method prepares acrylamide, acrylonitrile and
Water is prepared under the action of biocatalyst.
The technical solution adopted by the present invention is that：
A kind of biochemical method prepares the technique of acrylamide, using acrylonitrile and water as raw material, carries out under the action of biocatalyst
Hydration reaction, end of reaction is filtered, refines, crystallizing and drying process is prepared.
Further, the biocatalyst is strain catalyst.
Further, concrete operation step is as follows：
A, the culture of strain：It selects good quality strain tentatively to be cultivated first, then carries out mass propagation, it is dilute that breeding finishes centrifugation
It is stand-by after releasing；
B, the production of acrylamide：Strain after above-mentioned centrifugation dilution is added in acrylonitrile, hydration reaction is carried out, hydration is anti-
It has answered and reaction solution is filtered and obtains acrylamide aqueous solution after ionic adsorption；
C, the essence of acrylamide mentions：It will carry out being dried to obtain acrylamide crystal after above-mentioned acrylamide aqueous solution condensing crystallizing；
D, the recovery processing of catalyst：Waste liquid is isolated thallus by membrane separation technique to recycle.
Further, the mass ratio of strain and acrylonitrile is 0.08 in the step b:1-0.12:1.
Further, the strain is selected from saccharomycete and Bacillus acidi lactici.
Further, the concentration of the acrylonitrile is 99%.
The beneficial effects of the invention are as follows：1, using biocatalyst, environmental pollution is small, and recoverable, and reducing makes
Use cost；2, fully reacting, no coupling product, reaction condition are mildly, low energy consumption, the three wastes are few.
Specific embodiment
In order to deepen the understanding of the present invention, the present invention will be described in further detail with reference to the examples below, the embodiment
For explaining only the invention, it does not restrict the protection scope of the present invention.
A kind of biochemical method prepares the technique of acrylamide, using acrylonitrile and water as raw material, under the action of biocatalyst
Hydration reaction is carried out, end of reaction is filtered, refines, crystallizing and drying process is prepared, and biocatalyst is saccharomycete
Catalyst, specific operation process are as follows：
Embodiment 1
A, the culture of strain：Select good quality strain, be added in aqueous culture solution and tentatively cultivated, cultivation cycle be for 24 hours, and
The strain after preliminary culture is added to afterwards in the culture medium of aqueous nutrient solution and pure water composition and carries out mass propagation, the bread worm
It is 25-37 DEG C, breeding cycle 2-3d, mass propagation finishes, and takes out, it is put into the revolving speed in centrifuge with 12000r/min, from
Heart 30min, has been centrifuged plus pure water dilutes, and is prepared into strain catalyst；
B, the production of acrylamide：Above-mentioned strain catalyst 0.08Kg is added in the acrylonitrile of 1Kg, hydration reaction is carried out,
Reaction solution is filtered after hydration reaction, then to filtered fluid carry out ionic adsorption, after obtain acrylamide aqueous solution；
C, the essence of acrylamide mentions：It is carried out after above-mentioned acrylamide aqueous solution is evaporated condensing crystallizing by Rotary Evaporators
It is put into after being dried in drying box and obtains acrylamide crystal 150g；
D, the recovery processing of catalyst：The waste liquid being evaporated is separated by membrane separation technique, isolates thallus progress
It recycles.
Embodiment 2
A, the culture of strain：Select good quality strain, be added in aqueous culture solution and tentatively cultivated, cultivation cycle be for 24 hours, and
The strain after preliminary culture is added to afterwards in the culture medium of aqueous nutrient solution and pure water composition and carries out mass propagation, the bread worm
It is 25-37 DEG C, breeding cycle 2-3d, mass propagation finishes, and takes out, it is put into the revolving speed in centrifuge with 12000r/min, from
Heart 30min, has been centrifuged plus pure water dilutes, and is prepared into strain catalyst；
B, the production of acrylamide：Above-mentioned strain catalyst 0.12Kg is added in the acrylonitrile of 1Kg, hydration reaction is carried out,
Reaction solution is filtered after hydration reaction, then to filtered fluid carry out ionic adsorption, after obtain acrylamide aqueous solution；
C, the essence of acrylamide mentions：It is carried out after above-mentioned acrylamide aqueous solution is evaporated condensing crystallizing by Rotary Evaporators
It is put into after being dried in drying box and obtains acrylamide crystal 190g；
D, the recovery processing of catalyst：The waste liquid being evaporated is separated by membrane separation technique, isolates thallus progress
It recycles.
Embodiment 3
A, the culture of strain：Select good quality strain, be added in aqueous culture solution and tentatively cultivated, cultivation cycle be for 24 hours, and
The strain after preliminary culture is added to afterwards in the culture medium of aqueous nutrient solution and pure water composition and carries out mass propagation, the bread worm
It is 25-37 DEG C, breeding cycle 2-3d, mass propagation finishes, and takes out, it is put into the revolving speed in centrifuge with 12000r/min, from
Heart 30min, has been centrifuged plus pure water dilutes, and is prepared into strain catalyst；
B, the production of acrylamide：Above-mentioned strain catalyst 0.1Kg is added in the acrylonitrile of 1Kg, hydration reaction, water are carried out
Close reaction after reaction solution is filtered, then to filtered fluid carry out ionic adsorption, after obtain acrylamide aqueous solution；
C, the essence of acrylamide mentions：It is carried out after above-mentioned acrylamide aqueous solution is evaporated condensing crystallizing by Rotary Evaporators
It is put into after being dried in drying box and obtains acrylamide crystal 770g；
D, the recovery processing of catalyst：The waste liquid being evaporated is separated by membrane separation technique, isolates thallus progress
It recycles.
Embodiment 4
A, the culture of strain：Select good quality strain, be added in aqueous culture solution and tentatively cultivated, cultivation cycle be for 24 hours, and
The strain after preliminary culture is added to afterwards in the culture medium of aqueous nutrient solution and pure water composition and carries out mass propagation, the bread worm
It is 25-37 DEG C, breeding cycle 2-3d, mass propagation finishes, and takes out, it is put into the revolving speed in centrifuge with 12000r/min, from
Heart 30min, has been centrifuged plus pure water dilutes, and is prepared into strain catalyst；
B, the production of acrylamide：Above-mentioned strain catalyst 0.11Kg is added in the acrylonitrile of 1Kg, hydration reaction is carried out,
Reaction solution is filtered after hydration reaction, then to filtered fluid carry out ionic adsorption, after obtain acrylamide aqueous solution；
C, the essence of acrylamide mentions：It is carried out after above-mentioned acrylamide aqueous solution is evaporated condensing crystallizing by Rotary Evaporators
It is put into after being dried in drying box and obtains acrylamide crystal 180g；
D, the recovery processing of catalyst：The waste liquid being evaporated is separated by membrane separation technique, isolates thallus progress
It recycles.
Claims (6)
1. the technique that a kind of biochemical method prepares acrylamide, which is characterized in that using acrylonitrile and water as raw material, in biocatalyst
Under the action of carry out hydration reaction, end of reaction is filtered, refines, crystallizing and drying process is prepared.
2. the technique that a kind of biochemical method according to claim 1 prepares acrylamide, which is characterized in that the biocatalysis
Agent is strain catalyst.
3. the technique that a kind of biochemical method according to claim 1 prepares acrylamide, which is characterized in that concrete operation step
It is as follows：
A, the culture of strain：It selects good quality strain tentatively to be cultivated first, then carries out mass propagation, it is dilute that breeding finishes centrifugation
It is stand-by after releasing；
B, the production of acrylamide：Strain after above-mentioned centrifugation dilution is added in acrylonitrile, hydration reaction is carried out, hydration is anti-
It has answered and reaction solution is filtered and obtains acrylamide aqueous solution after ionic adsorption；
C, the essence of acrylamide mentions：It will carry out being dried to obtain acrylamide crystal after above-mentioned acrylamide aqueous solution condensing crystallizing；
D, the recovery processing of catalyst：Waste liquid is isolated thallus by membrane separation technique to recycle.
4. the technique that a kind of biochemical method according to claim 3 prepares acrylamide, which is characterized in that in the step b
The mass ratio of strain and acrylonitrile is 0.08:1-0.12:1.
5. the technique that a kind of biochemical method according to claim 3 prepares acrylamide, which is characterized in that the strain is selected from
Saccharomycete and Bacillus acidi lactici.
6. the technique that a kind of biochemical method according to claim 3 prepares acrylamide, which is characterized in that the acrylonitrile
Concentration is 99%.
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| CN201810731872.3A CN108913726A (en) | 2018-07-05 | 2018-07-05 | A kind of technique that biochemical method prepares acrylamide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112626141A (en) * | 2020-11-16 | 2021-04-09 | 广东宝莫生物化工有限公司 | Production method of acrylamide solution |
| CN115558685A (en) * | 2022-09-28 | 2023-01-03 | 南通博亿化工有限公司 | Process for producing acrylamide by microbiological method |
-
2018
- 2018-07-05 CN CN201810731872.3A patent/CN108913726A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112626141A (en) * | 2020-11-16 | 2021-04-09 | 广东宝莫生物化工有限公司 | Production method of acrylamide solution |
| CN112626141B (en) * | 2020-11-16 | 2022-12-06 | 广东宝莫生物化工有限公司 | Production method of acrylamide solution |
| CN115558685A (en) * | 2022-09-28 | 2023-01-03 | 南通博亿化工有限公司 | Process for producing acrylamide by microbiological method |
| CN115558685B (en) * | 2022-09-28 | 2023-09-29 | 南通博亿化工有限公司 | Process for producing acrylamide by microbiological method |
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