CN206354271U - Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism - Google Patents
Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism Download PDFInfo
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- CN206354271U CN206354271U CN201621456770.8U CN201621456770U CN206354271U CN 206354271 U CN206354271 U CN 206354271U CN 201621456770 U CN201621456770 U CN 201621456770U CN 206354271 U CN206354271 U CN 206354271U
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Abstract
The utility model is related to a kind of freezing carrier mechanism and freezing carrier unit, freezing carrier mechanism include holding structure and with the holding structure is connected freezes structure, the structure that freezes includes loading zone and capillarity arrangements, the loading zone is used to carry the cell, and the capillarity arrangements are used to produce capillarity to draw the immersion liquid of the loading zone.The freezing carrier mechanism produces capillarity by freezing structure; so as to which the most of cryoprotector for being coated on cell peripheral be siphoned away; only remain minimal amount of cryoprotector liquid film and be coated on cell peripheral; the suction process is completed by freezing carrier mechanism is automatic and spontaneous; independent of manually-operated control; realize the automatic minimum of cryoprotector covering amount; avoid the difference that technical merit difference is caused; so as to can reach the purpose of operational standardization control; and then effectively improve the survival rate and anabiosis rate of cell, it is ensured that its potentiality of development.
Description
Technical field
The utility model is related to biological technical field, more particularly to a kind of freezing carrier mechanism and including the freezing carrier
The freezing carrier unit of mechanism.
Background technology
Refrigeration Technique is the important content in auxiliary procreation technology link, and vitrification is the ovum of current main flow
Son/zygote/Embryo Cryopreservation Technology.Vitrification is made highly concentrated by the cooling rate (2000 DEG C/min of ＞) of superelevation
The cells such as ovum/zygote/embryo of degree cryoprotector cladding quickly form glassy state, it is to avoid intracellular generation ice crystal damage is thin
Born of the same parents, effectively improve ovum/zygote/embryo survival, anabiosis rate, it is ensured that its potentiality of development.In order to obtain the freezing speed of maximum
Rate, cryoprotector volume is the smaller the better.When cryoprotector covering amount is larger, freezing rate can be slack-off, and less freezing
Protective agent covering amount can shorten the time of heat transfer during freezing and rewarming.If freezing rate is slow, turn higher than vitrifying
The temperature (- 140 DEG C) of change has stop, it is possible to form intracellular ice crystal damage ovum/zygote/embryo.
Existing freezing carrier mechanism depends greatly on the technology water of operator in control cryoprotector volume
It is flat, need to carry out operator the quality control of corresponding special training and standardization before certain specifically used freezing carrier mechanism
System, but the difference between different operating person is still difficult to avoid that, even same operator, the skill under different time different conditions
The performance of art level can not realize the standardization control of the operation there is also difference.
Utility model content
Based on this, it is necessary to can not realize what cryoprotector covering amount was minimized automatically for above-mentioned freezing carrier mechanism
There is provided the freezing carrier mechanism that a kind of achievable cryoprotector covering amount is minimized automatically for problem.
Specific technical scheme is as follows：
A kind of freezing carrier mechanism, for carrying cell, the freezing carrier mechanism include holding structure and with it is described solid
The structure that freezes of structure connection is held, the structure that freezes includes loading zone and capillarity arrangements, and the loading zone is used to carry
The cell, the capillarity arrangements are used to produce capillarity to draw the immersion liquid of the loading zone.
The cell for being coated with cryoprotector when in use, can be transferred to and freeze adding for structure by above-mentioned freezing carrier mechanism
Area is carried, capillarity is produced by capillarity arrangements, so that the most of cryoprotector for being coated on cell peripheral is siphoned away,
Only remain minimal amount of cryoprotector liquid film and be coated on cell peripheral, the suction process is automatic and spontaneous complete by freezing carrier mechanism
Into independent of manually-operated control, realizing the automatic minimum of cryoprotector covering amount, it is to avoid technical merit is not
With the difference caused, so as to can reach the purpose of operational standardization control, and then ovum/zygote/embryo etc. is effectively improved
The survival rate and anabiosis rate of cell, it is ensured that its potentiality of development.
In one of the embodiments, the capillarity arrangements are provided with opening or the gap for being used for producing capillarity,
The loading zone is located at the chi in region of the capillarity arrangements provided with the opening or the gap, the opening or gap
The very little diameter less than the cell.
In one of the embodiments, the capillarity arrangements are tubular structure.
In one of the embodiments, the capillarity arrangements at least have two relative sides, and the capillary is made
Dead slot is offered sideways with least one of structure, so that the gap between described two relative sides forms the gap.
In one of the embodiments, the capillarity arrangements include being connected to extension and the company of the holding structure
Bending section of the extension away from described holding structure one end is connected to, the bending section bending extends, and the bending section does not connect
The end face for connecing the extension is provided with the opening, and is arranged at intervals with the extension and spacing distance is less than the cell
Diameter.
In one of the embodiments, the capillarity arrangements include filament and connecting tube, it is described it is filametntary extremely
Few one end is stretched into the connecting tube, so as to form the gap between the filament and the tube wall of the connecting tube.
In one of the embodiments, the filametntary quantity is at least two, and filament is parallel described at least two
And contact with each other.
In one of the embodiments, the capillarity arrangements are at least two filaments, fiber described at least two
The gap is formed between silk, described filament one end is connected with the holding structure.
In one of the embodiments, the structure that freezes also includes water suction bar, the water suction bar and the holding structure
Connection, the filament is connected to be connected with the holding structure by the water suction bar with the water suction bar.
Another object of the present utility model is to provide a kind of freezing carrier unit, including described freezing carrier mechanism and
Sleeve pipe, the freezing carrier mechanism is contained in described sleeve pipe and can be axially moved along described sleeve pipe, and described sleeve pipe is provided with axially
Position limiting structure freezes the ultimate range that structure place direction is moved to limit the freezing carrier mechanism to described.
Brief description of the drawings
Fig. 1 is the structural representation of the freezing carrier mechanism of first embodiment；
Fig. 2 is the structural representation of the freezing carrier mechanism of second embodiment；
Fig. 3 is to freeze the structural representation of structure in the freezing carrier mechanism shown in Fig. 1；
Fig. 4 is the partial enlarged drawing at A in Fig. 3；
Fig. 5 is freezes the structural representation of structure in the freezing carrier mechanism of 3rd embodiment；
Fig. 6 is the partial enlarged drawing at B in Fig. 5；
Fig. 7 is freezes the structural representation of structure in the freezing carrier mechanism of fourth embodiment；
Fig. 8 is the partial enlarged drawing at C in Fig. 7；
Fig. 9 is freezes the structural representation of structure in the freezing carrier mechanism of the 5th embodiment；
Figure 10 is the partial enlarged drawing at D in Fig. 9；
Figure 11 is freezes the structural representation of structure in the freezing carrier mechanism of sixth embodiment；
Figure 12 is the partial enlarged drawing at E in Figure 11；
Figure 13 is freezes the structural representation of structure in the freezing carrier mechanism of the 7th embodiment；
Figure 14 is the partial enlarged drawing at F in Figure 13；
Figure 15 is freezes the structural representation of structure in the freezing carrier mechanism of the 8th embodiment；
Figure 16 is the partial enlarged drawing at G in Figure 15；
Figure 17 is freezes the structural representation of structure in the freezing carrier mechanism of the 9th embodiment；
Figure 18 is the partial enlarged drawing at H in Figure 17；
Figure 19 is freezes the structural representation of structure in the freezing carrier mechanism of the tenth embodiment；
Figure 20 is the partial enlarged drawing at I in Figure 19；
Figure 21 is the structural representation of the freezing carrier unit of an embodiment；
Figure 22 is the sleeve pipe and the structural representation of pipe cap of the freezing carrier unit shown in Figure 21.
Embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
State.Preferred embodiment of the present utility model is given in accompanying drawing.But, the utility model can come real in many different forms
It is existing, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is made to public affairs of the present utility model
The understanding for opening content is more thorough comprehensive.
It should be noted that when element is referred to as " being installed on " another element, it can be directly on another element
Or can also have element placed in the middle.When an element is considered as " connection " another element, it can be direct connection
To another element or it may be simultaneously present centering elements.When an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to technology of the present utility model
The implication that the technical staff in domain is generally understood that is identical.It is herein to be in term used in the description of the present utility model
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
The arbitrary and all combination of one or more related Listed Items.
As shown in figure 1, a kind of freezing carrier mechanism 100 of first embodiment of the present utility model, including holding structure 110
With freeze structure 120.Holding structure 110 is connected with freezing structure 120, and freezing structure 120 includes loading zone and capillarity knot
Structure 121, loading zone is used to carry cell, and capillarity arrangements 121 are used to produce capillarity to draw the immersion fluid of loading zone
Body.
The cell for being coated with cryoprotector when in use, can be transferred to jelly by the freezing carrier mechanism 100 of the present embodiment
The loading zone of structure 120 is deposited, capillarity is produced by capillarity arrangements 121, so that the big portion that cell peripheral will be coated on
Divide cryoprotector to siphon away, only remain minimal amount of cryoprotector liquid film and be coated on cell peripheral.The suction process is carried by freezing
Body mechanism 100 is automatic and spontaneous to be completed, and independent of manually-operated control, realizes the automatic minimum of cryoprotector covering amount
Change, it is to avoid the difference that technical merit difference is caused, so as to can reach the purpose of operational standardization control, and then effectively carry
The survival rate and anabiosis rate of high ovum/zygote/embryo, it is ensured that its potentiality of development.
In the present embodiment, freezing carrier mechanism 100 also includes falling 114 again, 114 is fallen again remote located at holding structure 110
One end of structure 120 is frozen, the weight for increasing freezing carrier 100 improves stability.Specifically, holding structure 110 includes
Shank body 111 for holding operation, the one end of shank body 111 be provided with freeze that structure 120 is connected freeze structural connection 112, handle
The other end of body 111 is used for fixation provided with pendant fixed part 113 again and falls 114 again.In the present embodiment, holding structure 110 is with freezing knot
Structure 120 is integrally formed, the freezing carrier mechanism 200 of second embodiment that also can be as shown in Figure 2, is formed by connecting by each several part.
Preferably, structure 120 is frozen using TC (Tissue culturetreated) processing, is improved wellability, is strengthened hair
Thin action effect.Holding structure 110 is made up of materials such as low temperature resistant high polymer material, metal, glass or carbon fibers.Freeze structure
120 are made up of materials such as transparent low temperature resistant high polymer material, glass, metals.
As shown in Figure 3 and Figure 4, in the first embodiment, capillarity arrangements 121, which are provided with, is used to produce opening for capillarity
Mouth 1211 or gap, loading zone are located at region of the capillarity arrangements 121 provided with opening 1211 or gap, and opening 1211 or seam
The size of gap is less than the diameter to be carried cell.In this way, when glass freezing is operated, by cell and the cold of cell can be coated
Freeze protective agent and be transferred to loading zone positioned at the region provided with opening 1211 or gap, cryoprotector touch opening 1211 or
Siphoned away automatically by the way that capillarity is most of behind gap, but cell can not be siphoned away, realize cryoprotector covering amount oneself
It is dynamic to minimize.
Preferably, capillarity arrangements 121 are tubular structure, end face of the capillarity arrangements 121 away from holding structure 110
Provided with the inclined-plane being obliquely installed, opening 1211 is located at the inclined-plane.Specifically, the size of capillarity arrangements 121 is to away from fixing
The one end of structure 110 is gradually reduced, such as 10 degree at an acute angle, 20 degree of the axis on inclined-plane and capillarity arrangements 121, capillarity knot
External diameter is 300 μm at one end for the institute of opening 1211 of structure 121, and the diameter of opening 1211 is less than the diameter such as 50 of ovum/zygote/embryo
μm, the other end external diameter away from opening 1211 is 2.0mm, and the amount of liquid that capillarity arrangements 121 can receive is more than 1 μ L.At this
In embodiment, the inclined-plane and opening 1211 for offering opening 1211 are oval.It is appreciated that capillarity arrangements 121
Size and dimension not limited to this, cross section can be that the capillary in circle, 3rd embodiment that also can be as shown in Figure 5 and Figure 6 is made
With structure 121, inclined-plane and opening 1211 provided with opening are rectangular.
The freezing carrier mechanism 100 of the present embodiment produces capillarity by freezing structure 120, so that cell will be coated on
Most of cryoprotector of surrounding is siphoned away, and is only remained minimal amount of cryoprotector liquid film and is coated on cell peripheral, this was drawn
Journey is completed by freezing carrier mechanism 100 is automatic and spontaneous, independent of manually-operated control, realizes cryoprotector covering amount
Automatic minimum, can reach the purpose of operational standardization control, and then effectively improve the survival of ovum/zygote/embryo
Rate and anabiosis rate, it is ensured that its potentiality of development.
As shown in Figure 7 and Figure 8, the freezing carrier mechanism for the fourth embodiment that the utility model is provided includes implementing with first
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example, difference is, in this reality
Apply in example, the capillarity arrangements 421 of tubular structure at least have two relative sides, and capillarity arrangements 421 are at least
One side offers dead slot 4213, so that the gap between two relative sides forms the gap for producing capillarity
4212, the size in gap 4212 is less than the diameter to be carried cell, and loading zone is located at the formation of capillarity arrangements 421 gap
4212 region.In this way, when glass freezing is operated, the cryoprotector of cell and cladding cell can be transferred into loading
Area, cryoprotector is touched and largely siphoned away automatically by capillarity behind gap 4212, but can not siphon away cell, real
The automatic minimum of existing cryoprotector covering amount.
It is appreciated that the shape of dead slot 4213 can be arranged as required to, the dead slot 4213 in the present embodiment is c-type or circle
Shape, can also be located at multiple sides, can also be formed located at the correspondence position of two relative sides only located at a side
Through hole.Specifically in the present embodiment, the rounded rectangle in cross section of capillarity arrangements 421, and the width of the rectangle is less than institute
Carry the diameter of cell.Circular dead slot can be opened in side where wherein one or two long side of capillarity arrangements 421
Middle part and rounded, the edge of side where C-shaped dead slot can be opened in one or two long side.
As shown in Figure 9 and Figure 10, the freezing carrier mechanism for the 5th embodiment that the utility model is provided is included with first in fact
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example is applied, difference is, at this
In embodiment, the capillarity arrangements 521 of tubulose include being connected to the extension 5213 of holding structure and are connected to extension
5213 bending sections 5214 away from holding structure one end, the bending extension of bending section 5214, bending section 5214 is not connected with extension
5213 end face is offered for producing the opening 5211 of capillarity, and is arranged at intervals with extension 5213 and spacing distance is small
In the diameter to be carried cell, the size of opening 5211 is less than the diameter to be carried cell, and loading zone is located at capillarity
Structure 521 is provided with the region of opening 5211.
In this way, when glass freezing is operated, the cryoprotector of cell and cladding cell can be transferred into bending section
5214 loading zone, cryoprotector is touched and largely siphoned away automatically by capillarity after opening 5211, is realized cold
Freeze the automatic minimum of protective agent covering amount, and due to the place end face of opening 5211 and capillarity arrangements 521 lateral wall away from
From the diameter less than the cell to be carried, it can avoid being blocked by cell in the protectant process split shed 5211 of absorption freezing
Wicking process is caused to terminate in advance.
It is appreciated that the form and size of bending section 5214 can be arranged as required to, and in the present embodiment, bending section 5214
In the ring-type of ρ shapes, length 10mm, overall diameter is close to such as 120 μm of the external diameter of ovum/zygote/embryo, and the bore of opening 5211 is less than
Such as 50 μm of the external diameter of ovum/zygote/embryo, 5211 place end faces of opening are outer with the extension 5213 of capillarity arrangements 521
Sidewall spacers distance is less than 50 μm.
As is illustrated by figs. 11 and 12, the freezing carrier mechanism for the sixth embodiment that the utility model is provided is included with first in fact
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example is applied, difference is, at this
In embodiment, capillarity arrangements 621 include filament 6213 and connecting tube 6214, and company is stretched at least one end of filament 6213
In adapter 6214, so as to form the gap for producing capillarity between filament 6213 and the tube wall of connecting tube 6214
6212, the size in gap 6212 is less than the diameter to be carried cell, and loading zone is located at the formation of capillarity arrangements 621 gap
6212 region.
In this way, when glass freezing is operated, can be first by filament 6213 and the end cryoprotection of connecting tube 6214
Agent infiltrates, so that the surface of filament 6213 covers liquid film, the gap between filament 6213 and the tube wall of connecting tube 6214
6212 have sucked a little cryoprotector, can strengthen capillary action effect, then by cell and the cryoprotection of cladding cell
Agent is transferred to the loading zone of filament 6213, and cryoprotector touches i.e. most of by certainly by capillarity behind gap 6212
It is dynamic to siphon away, realize the automatic minimum of cryoprotector covering amount.
In the present embodiment, connecting tube 6214 is wholely set with holding structure, and the internal diameter of connecting tube 6214 is 150 μm, fine
The external diameter for tieing up silk 6213 is 100 μm, and the partial-length that filament 6213 is located at outside connecting tube 6214 is 2mm, it will be understood that size
Form not limited to this, can be adjusted as needed.
As shown in Figure 13 and Figure 14, the freezing carrier mechanism for the 7th embodiment that the utility model is provided is included with first in fact
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example is applied, difference is, at this
In embodiment, capillarity arrangements 721 include filament 7213 and connecting tube 7214, and company is stretched at least one end of filament 7213
In adapter 7214, so as to form the gap for producing capillarity between filament 7213 and the tube wall of connecting tube 7214
7212, the size in gap 7212 is less than the diameter to be carried cell, and the quantity of filament 7213 is at least two, at least two
Individual filament 7213 is parallel and contacts with each other, and loading zone is located at the region in the formation of capillarity arrangements 721 gap 7212.
In this way, between filament 7213 can drainage cryoprotector, can be first by filament when glass freezing is operated
7213 and connecting tube 7214 stretch into frozen solution infiltrate so that the surface of filament 7213 covers liquid film, filament
Cryoprotector has been sucked between 7213 makes gap smaller, the gap 7212 between filament 7213 and the tube wall of connecting tube 7214
A little cryoprotector is sucked, capillary action effect is further enhancing.Then by filament 7213 be lifted away from frozen solution or
Person makes the end of filament 7213 keep contacting with frozen solution, then the cryoprotector of cell and cladding cell is transferred to
The loading zone of filament 7213, cryoprotector is drained to the cold of connecting tube 7214 or the one end of filament 7213 by filament 7213
Freeze protection liquid, most of cryoprotector is sucked away immediately, realizes the automatic minimum of cryoprotector covering amount.
Preferably, at least one filament 7213 is hard state fibers straight, such as hard state stainless steel fibers straight.In the present embodiment
In, the quantity of filament 7213 is 2, and the external diameter of filament 7213 is 40 μm, and the internal diameter of connecting tube 7214 is slightly larger than filament
Such as 100 μm of 7213 external diameter sum.It is appreciated that size modes not limited to this, can be adjusted as needed.
As shown in Figure 15 and Figure 16, the freezing carrier mechanism for the 8th embodiment that the utility model is provided is included with first in fact
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example is applied, difference is, at this
In embodiment, capillarity arrangements 821 include filament 8213 and connecting tube 8214, and connection is stretched at the two ends of filament 8213
Pipe 8214, so that the part that filament 8213 is located at outside connecting tube 8214 forms cyclic structure, and filament 8213 is with being connected
The gap 8212 for producing capillarity is formed between the tube wall of pipe 8214, the size in gap 8212 is less than to be carried cell
Diameter, loading zone be located at capillarity arrangements 821 formation gap 8212 region.
In this way, when glass freezing is operated, first can infiltrate filament 8213 and connecting tube 8214 with cryoprotector,
Then the cryoprotector of cell and cladding cell is transferred to the loading zone formation drop or liquid film of cyclic structure, cryoprotection
Agent touches gap 8212 and is largely sucked away by capillarity immediately, realizes the automatic minimum of cryoprotector covering amount
Change.In the present embodiment, the external diameter of filament 8213 is 50 μm, and the internal diameter of connecting tube 8214 is slightly larger than the external diameter of filament 8213
Twice such as 110 μm.
As shown in Figure 17 and Figure 18, the freezing carrier mechanism 900 for the 9th embodiment that the utility model is provided includes and the
The identical holding structure 910 of holding structure 110 in the freezing carrier mechanism 100 of one embodiment, difference is, in this reality
Apply in example, capillarity arrangements 921 are to be formed between at least two filaments 9213, at least two filaments 9213 for producing
The gap 9212 of capillarity, the size in gap 9212 is less than the diameter to be carried cell, the one end of filament 9213 and fixing
Structure is connected, and loading zone is located at the region in the formation of capillarity arrangements 921 gap 9212.
In this way, when glass freezing is operated, first filament 9213 can be stretched into frozen solution and infiltrated, then raised and make
The end of filament 9213 is kept contacting with frozen solution, and cell and the cryoprotector for coating cell are transferred into filament
9213 loading zone, the gap 9212 of most of cryoprotector then between filament 9213 is drawn to freezing by capillarity and protected
Liquid is protected, the automatic minimum of cryoprotector covering amount is realized.
Preferably, at least one filament 9213 is hard state fibers straight, such as hard state stainless steel fibers straight.In the present embodiment
In, the length of filament 9213 is 4mm, and quantity is 2, and external diameter is 40 μm, it will be understood that size modes not limited to this, many
The external diameter of individual filament 9213 also can be inconsistent, is adjusted as needed.
As illustrated in figures 19 and 20, the freezing carrier mechanism for the tenth embodiment that the utility model is provided is included with first in fact
The identical holding structure (not shown) of holding structure 110 in the freezing carrier mechanism 100 of example is applied, difference is, at this
In embodiment, freezing structure 1020 includes loading zone, capillarity arrangements 1021 and water suction bar 1022, capillarity arrangements 1021
For at least two filaments 10213, the gap 10212 for producing capillarity is formed between at least two filaments 10213,
The size in gap 10212 is less than to be carried the diameter of cell, and water suction bar 1022 is connected with holding structure, filament 10213 and
Water suction bar 1022 is connected to be connected by the bar 1022 that absorbs water with holding structure, and loading zone is located at the formation seam of capillarity arrangements 1021
The region of gap 10212.
In this way, when glass freezing is operated, filament 10213 and part water suction bar 1022 first can be used into frozen solution
Infiltration, then be lifted away from frozen solution or make the end of filament 10213 keep contacting with frozen solution, then by cell and
The cryoprotector of cladding cell is transferred to the loading zone of filament 10213, and most of cryoprotector passes through filament immediately
Gap 10212 between 10213 is drained into the water suction bar 1022 of one end or the frozen solution of the other end, and water suction bar 1022 is effective
Ground enhances capillary action effect, realizes the automatic minimum of cryoprotector covering amount.
As shown in figure 21 and figure, a kind of freezing carrier unit 500, including above-mentioned freezing carrier mechanism 100 and sleeve pipe
300, freezing carrier mechanism 100 is contained in sleeve pipe 300 and can be axially moved along sleeve pipe 300, and axial limiting is provided with sleeve pipe 300
Structure (not shown) is to limit freezing carrier mechanism 100 to freezing the ultimate range that the place direction of structure 20 is moved.
The freezing carrier unit 500 of the present embodiment, its freezing carrier mechanism 100 is contained in sleeve pipe 300, can be effective
Freezing carrier mechanism 100 is protected, it is smaller especially to freeze structure 120, collides and is easily deformed or fractures, therefore can prevent fortune
Damage during defeated storage.Meanwhile, freezing carrier mechanism 100 can be axially moved along sleeve pipe 300, and have axial limit structure limit
Move distance processed, so as to need to only tilt certain angle, you can make freezing carrier mechanism 100 under gravity freezes structure
120 skid off outside sleeve pipe 300 or return in sleeve pipe 300, without being manually inserted in freezing carrier mechanism 100 during freeze-thaw
Sleeve pipe 300 takes out, and causes freezing carrier mechanism 100 to collide and be damaged with sleeve pipe 300 so as to avoid operational error, makes
With more convenient, safety.
Specifically, axial limit structure is the contraction mouth that one end internal wall of pipe orifice of sleeve pipe 300 inwardly reduces formation, or is sleeve pipe
Axially arranged annulus in 300 one end mouths of pipe, freezing load is can control by adjusting the external diameter of different parts of holding structure 110
The ultimate range that body mechanism 100 is axially moved along sleeve pipe 300.It is appreciated that the structure not limited to this of axial limit structure, can root
According to needing to set, for example, it is arranged on holding structure 110 and is provided with and can be mounted with the annulus in the annulus with through hole, sleeve pipe 300
Hook.
In the present embodiment, the cylindrical structure of sleeve pipe 300, including casing main body 301 and it is respectively arranged on casing main body 301
The pullover 302 and set tail 303 at two ends, freeze structure 120 close to pullover 302, set tail 303 is that open end such as passes through sleeve pipe master
The one end of body 301 reduces to form contraction mouth or filling non-hermetic material or closed type terminal such as by casing main body 301
One end reduces to form cecum or filling sealing material；The minimum diameter of axial limit structure is more than the maximum for freezing structure 120
External diameter, footpath difference is 0.5mm；The minimum diameter of axial limit structure is less than the maximum outside diameter of holding structure 110, and footpath difference is 0.5mm；
The length of freezing carrier mechanism 100 is less than the length of the inner chamber of casing main body 301, and length difference is 3mm；Freezing carrier mechanism 100
Maximum outside diameter is less than the internal diameter of casing main body 301, and footpath difference is 0.5mm.It is appreciated that size modes not limited to this, can be according to need
Adjust.
Preferably, freezing carrier unit 500 also includes pipe cap 400, and pipe cap 400 can be sheathed on sleeve pipe 300 close to freezing knot
One end of structure 20, so as to further strengthen the protection to freezing structure 120.
In the present embodiment, pipe cap 400 is cylindrical, including pipe cap main body 401 and is respectively arranged on the two ends of pipe cap main body 401
Cap head 402 and cap tail 403, cap head 402 radially extends in infundibulate so as to being play the guiding role when being sheathed on sleeve pipe 300, covers
Pipe 300 is connected with the interference of pipe cap 400；Cap tail 403 is that open end such as reduces to form contraction mouth by the one end of pipe cap main body 401
Or filling non-hermetic material or closed type terminal such as reduce to form cecum or filling by the one end of pipe cap main body 401
Sealing material；The internal diameter of pipe cap main body 401 is slightly less than the external diameter of sleeve pipe 300, and footpath difference is 0.1mm；The maximum outside diameter of cap head 402
More than the external diameter of pullover 302, footpath difference is 0.2mm.It is appreciated that size modes not limited to this, can be adjusted as needed.
Preferably, the lateral wall of sleeve pipe 300 is provided with pipe cap position limiting structure 310, and sleeve pipe 300 is sheathed on to limit pipe cap 400
Depth capacity, so as to prevent from when freezing carrier 100 is axially moved colliding with the bottom of pipe cap 400.It is appreciated that mode
Not limited to this, for example, can also set warning line, or the internal diameter of control pipe cap 400 to change in the lateral wall of sleeve pipe 300.At this
In embodiment, freezing carrier mechanism 100, axially to when freezing the place direction of structure 120 and moving to ultimate range, freezes along sleeve pipe 300
Deposit structure 120 and be sheathed on the bottom surface of the pipe cap 400 of sleeve pipe 300 apart from 3mm.
The freezing carrier unit 500 of the present embodiment, its freezing carrier mechanism 100 is contained in sleeve pipe 300,400 sets of pipe cap
Located at sleeve pipe 300, effective protection is provided to freeze structure 120, freezing carrier mechanism 100 can be axially moved along sleeve pipe 300, and
There is axial limit structure to limit largest motion distance, so as to need to only tilt certain angle, you can carry freezing under gravity
The structure 120 that freezes of body mechanism 100 is skidded off outside sleeve pipe 300 or returned in sleeve pipe 300, without manually will during freeze-thaw
Sleeve pipe 300 or taking-up are inserted in freezing carrier mechanism 100, so that avoiding operational error causes freezing carrier mechanism 100 and sleeve pipe
300 collide and are damaged, and use more convenient, safety.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses several embodiments of the present utility model, and it describes more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent scope.It should be pointed out that for the common skill of this area
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
Protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of freezing carrier mechanism, for carrying cell, it is characterised in that the freezing carrier mechanism include holding structure and
What is be connected with the holding structure freezes structure, and the structure that freezes includes loading zone and capillarity arrangements, the loading zone
For carrying the cell, the capillarity arrangements are used to produce capillarity to draw the immersion liquid of the loading zone.
2. freezing carrier mechanism according to claim 1, it is characterised in that the capillarity arrangements, which are provided with, to be used to produce
The opening of capillarity or gap, the loading zone are located at area of the capillarity arrangements provided with the opening or the gap
The size in domain, the opening or gap is less than the diameter of the cell.
3. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements are tubular structure.
4. freezing carrier mechanism according to claim 3, it is characterised in that the capillarity arrangements at least have two
Relative side, at least one of the capillarity arrangements offers dead slot sideways so that described two relative sides it
Between gap form the gap.
5. freezing carrier mechanism according to claim 3, it is characterised in that the capillarity arrangements include being connected to institute
State the extension of holding structure and be connected to bending section of the extension away from described holding structure one end, the bending section is curved
Qu Yanshen, the bending section be not connected with the extension end face be provided with the opening, and with the extension interval setting and
Spacing distance is less than the diameter of the cell.
6. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements include filament and
Connecting tube, described filametntary at least one end is stretched into the connecting tube, so that the filament and the tube wall of the connecting tube
Between form the gap.
7. freezing carrier mechanism according to claim 6, it is characterised in that the filametntary quantity is at least two,
Filament described at least two is parallel and contacts with each other.
8. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements are at least two fine
Tie up and form the gap between silk, filament described at least two, described filament one end is connected with the holding structure.
9. freezing carrier mechanism according to claim 8, it is characterised in that the structure that freezes also includes water suction bar, institute
State water suction bar be connected with the holding structure, the filament with it is described absorb water bar be connected with by it is described absorb water bar with it is described solid
Hold structure connection.
10. a kind of freezing carrier unit, it is characterised in that including the freezing carrier mechanism as described in claim any one of 1-9
And sleeve pipe, the freezing carrier mechanism is contained in described sleeve pipe and can be axially moved along described sleeve pipe, and described sleeve pipe is provided with axle
To position limiting structure the ultimate range that structure place direction is moved is frozen to limit the freezing carrier mechanism to described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621456770.8U CN206354271U (en) | 2016-12-28 | 2016-12-28 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621456770.8U CN206354271U (en) | 2016-12-28 | 2016-12-28 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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| Publication Number | Publication Date |
|---|---|
| CN206354271U true CN206354271U (en) | 2017-07-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
-
2016
- 2016-12-28 CN CN201621456770.8U patent/CN206354271U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
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