CN109082363A - A kind of preferred sperm freezing culture device - Google Patents
A kind of preferred sperm freezing culture device Download PDFInfo
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- CN109082363A CN109082363A CN201810813651.0A CN201810813651A CN109082363A CN 109082363 A CN109082363 A CN 109082363A CN 201810813651 A CN201810813651 A CN 201810813651A CN 109082363 A CN109082363 A CN 109082363A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
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Abstract
The present invention relates to a kind of preferred sperm freezing culture device, the culture device is equipped with frost slide glass, seminal pool, sperm travelling channel, collecting pit, barrier sheet；The sperm pool volume is 50 to 500 microlitres；The collection pool volume is 5 to 20 microlitres；The width in the sperm travelling channel is 10-30 microns；Sperm travelling channel connection seminal pool and collecting pit.The lifter have the advantages that the screening for being suitable for frozen sperm saves, motile sperm can be automatically separated using the design of seminal pool, sperm travelling channel, collecting pit, reduce operating quantity, avoid hand picking to the mechanical damage of sperm, improve the ratio of available active sperm, the frost slide glass of existing all sperms can be replaced completely, applicable field has: entering that Periodic Traveling IVF, ICSI or artificial insemination, few weak smart patient collects sperm, azoospermia patients operation takes essence in the preferred sperm of normal semen, collects sperm etc..It has broad popularization and application prospect.
Description
Technical field
The present invention relates to the field of medical instrument technology, specifically, being a kind of preferred sperm freezing culture.
Background technique
Vitro fertilization-embryo implanting (IVF-ET) is also known as " test-tube baby ", refers to respectively by ovum and sperm out of human body
It takes out and is fertilized in vitro, after developing into embryo, then transplant back in maternal uterine, to reach a kind of technology for purpose of becoming pregnant.
ICSI (Intracytoplasmic sperm injection) i.e. intracytoplasmic sperm injection in treating infertility, that is, second
In generation " test-tube baby ", which is to enter to make in ovum its fertilization for single sperm injection by micromanipulation system, these technologies
When carrying out, the period is very long or some patients do not have the condition of raising child at this time, needs first frozen sperm, remains with laggard
Row artificial insemination or test-tube baby.
Currently, existing more than 200 years history of semen cryopreservation technology.The semen cryopreservation of people can trace back to 20 generation
It records the end of the forties.Semen cryopreservation is mainly used in the preservation of human sperm bank and fecundity in China.
Conventional sperm freezing method use the cryovial of 2ml, sperm and freezing liquid by after 1:1 dilution proportion through liquid nitrogen smoked
Liquid nitrogen frozen preservation is put into after steaming again.Since intracellular ice crystal the factors such as forms and will lead to a degree of sperm in refrigerating process
Damage, mechanical damage when along with after defrosting to sperm progress hand picking, the sperm quantity for having vigor can be greatly reduced,
And it is time-consuming and laborious, and can only accomplish to get off sperm storage for rare sperm freezing, based at present to rare sperm freezing
It saves that anabiosis rate is not high or even after patient man and wife enters the period, the motile sperm quantity found of recovering is less than the ovum that the wife's side obtains
Subnumber amount after some patient's sperm freezings, can not find motile sperm after recovery.
If it is known that the state (activity or motionless) before sperm freezing, starches interior single essence for reproductive center row ooecium
Sub- microinjection technique (ICSI) has decisive help.
Chinese patent literature CN:CN201010160927 discloses a kind of inner sleeve type separation screening sperm tube, including outer
Pipe, outer pipe bottom install inner tube, and inner tube one end is in outer tube, and the other end is outside outer tube；Outlet tube is set on outer tube, on outlet tube
Installation unscrewing valve.It has outer tube and inner tube, and sperm is placed in inner tube, fills nutrient solution in outer tube, is extracted by outer tube containing high living
When the nutrient solution of power sperm, former sperm liquid level can be made in inner tube interference-free, prevent from extracting the nutrition for containing high motile sperm
When liquid, former sperm component portion is inhaled into the liquid of upstream, improves the quality of screening sperm.The present invention also has structure simple
Compact, cheap and using simplicity the advantage of manufacturing cost.But about a kind of features simple structure, motile sperm and not can be voluntarily separated
Dynamic sperm, the sperm culture device that can also be directly used in freezen protective have not been reported.
In conclusion it is simple to need a kind of structure, can be used for frozen sperm preservation, can voluntarily separate motile sperm and
The culture device of spermatium, and this slide glass yet there are no disclosure
Summary of the invention
It, can be with the purpose of the present invention is aiming at the shortcomings in the prior art, providing a kind of preferred sperm freezing culture device
It is automatically separated motile sperm and spermatium, avoids the mechanical damage of hand picking, especially for the sperm of rare sperm patient,
The quantity for finding motile sperm can be increased, and can be directly used for liquid nitrogen frozen preservation.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of preferred sperm freezing culture device, the freezing culture device include frost slide glass, seminal pool, sperm travelling
Channel, collecting pit, barrier sheet；The width in the sperm travelling channel is 10-30 microns；In the barrier sheet and frost slide glass
The slype that surface engagement is formed is sperm travelling channel；Sperm travelling channel connection seminal pool and collecting pit.
As a kind of perferred technical scheme, the barrier sheet and seminal pool, collecting pit do surface coating processing.
As a kind of perferred technical scheme, sperm travelling channel connection seminal pool part is than connection collecting pit part
Opening is spacious.
As a kind of perferred technical scheme, the barrier sheet is fixed on frost slide glass, is integrally formed structures.
As a kind of perferred technical scheme, the frost slide glass and cover plate are made using polystyrene material.
As a kind of perferred technical scheme, larger, the small shape in abdominal cavity of the seminal pool opening；The collecting pit is to open
Small, the big shape in abdominal cavity of mouth.
As a kind of perferred technical scheme, the volume of the seminal pool is greater than the volume of collecting pit.
As a kind of perferred technical scheme, the sperm pool volume is 2 to 3 milliliters；The sperm pool volume be 50 to
500 microlitres；The collection pool volume is 5 to 20 microlitres.
As a kind of perferred technical scheme, the seminal pool, collecting pit and sperm travelling channel are and frost
What slide glass was detachably connected.
As a kind of perferred technical scheme, the inner wall of the seminal pool, collecting pit and sperm travelling channel is adopted
It is designed with arcuate structure.
The invention has the advantages that:
1, a kind of preferred sperm freezing culture device of the present invention uses simple, utilizes seminal pool, sperm travelling channel, collects
The design in pond can be automatically separated motile sperm, reduce operating quantity, avoid the mechanical damage to sperm of hand picking, improving can
With the ratio of motile sperm, the frost slide glass of existing all sperms can be replaced completely, applicable field has: in normal semen
It is preferred that sperm enters, Periodic Traveling IVF, ICSI or artificial insemination, few weak smart patient collects sperm, azoospermia patients operation takes essence,
Collect sperm etc..It has broad popularization and application prospect.
2, slide glass is made using polystyrene material, and intensity is high, non-friable, has good bio-compatibility.
3, coating designs are used inside frost slide glass, reduces the frictional force of sperm and slide glass inner surface, reduced sperm and stick.
Seminal pool is designed to the big structure that is open, and sperm is facilitated to swim out of, and collecting pit opening is small, and abdominal cavity is big, and sperm is not easy to swim out of, and improves
Collection rate.
4, this slide glass can directly freezed in liquid nitrogen, separated sperm can also be transferred to other frost slide glasses in freeze.
5. applying this slide glass frozen sperm, sperm clearly can be divided into two classes and frozen, is i.e. motile sperm freezing drop, motionless essence
Son freezing drop, using need not select after recovery.
6. sperm moves about channel cannot be excessive, only need simultaneously can side by side by 5-10 sperm can (30-50 microns), it is no
Then sperm can swim back seminal pool from collecting pit.
7. frost slide glass is made using polystyrene material, hydrophily, freexing tolerance are very good.
8. freezing culture device of the invention, be for few weak smart patient, the dimensions of seminal pool or collecting pit volume
It can design as needed, different model can be formed.
Detailed description of the invention
Attached drawing 1 is a kind of frost slide structure figure of preferred sperm freezing culture device of the present invention.
Attached drawing 2 is slide glass side perspective view.
Specific embodiment
It elaborates with reference to the accompanying drawing to specific embodiment provided by the invention.
Appended drawing reference involved in attached drawing and component part are as follows:
1. freezing 11. seminal pool of slide glass
13. collecting pit of channel 12. sperm moves about
14. barrier sheet
Embodiment 1
Please referring to Fig. 1, Fig. 1 is a kind of frost slide structure figure of preferred sperm freezing culture device of the present invention, described cold
Freezing culture device includes frost slide glass 1, seminal pool 11, sperm travelling channel 12, collecting pit 13, barrier sheet 14；The seminal pool
11 volumes are 50 to 500 microlitres；The collection pool volume is 5 to 20 microlitres；The inner surface of the barrier sheet 14 and slide glass cooperates
The gap of formation is sperm travelling channel 12.
Referring to figure 2., Fig. 2 is slide glass side perspective view.The barrier sheet 14 cooperatively forms narrow logical with frost slide glass 1
Road is sperm travelling channel 12；Sperm travelling channel 12 connects seminal pool 11 and collecting pit 13；Sperm travelling channel
It is more spacious than connection 13 part of collecting pit to connect 11 part of seminal pool；The width in the sperm travelling channel 12 is 10-30 microns.
It should be understood that
The slide glass is made using polystyrene material, has good bio-compatibility and compressive property.
The seminal pool 11 is the semi-cylindrical laid flat, is open larger, abdominal cavity is small, and sperm is facilitated to swim out of；The sperm trip
The inclined bottom surface in dynamic channel 12, connection 13 one end of collecting pit are higher than connection 11 end of seminal pool；The sperm travelling channel 12 and receipts
Connection end opening in collection pond 13 is narrow, and sperm trip comes in be easy, and it is lower to swim out of probability；The collecting pit 13 is small, the abdominal cavity that is open
Big shape is conducive to collect sperm.
14 width of barrier sheet and the equivalent width for freezing seminal pool 11 on slide glass 1,13 place rectangular depression of collecting pit.
The volume of the seminal pool 11 is greater than the volume of collecting pit 13, which can be collected into convenient for collecting pit 13
Sufficiently enough sperms.
Seminal pool 11, collecting pit 13 and the inner wall in sperm travelling channel 12 is all made of arcuate structure design.It fills
Divide and tension principle existing for seminal fluid is utilized, arcuate structure design, so that flow of semen is quick.
The barrier sheet 14 and seminal pool 11, collecting pit 13 do surface coating processing, prevent sperm from attaching to surface.
The barrier sheet 14 is fixed on frost slide glass, is integrally formed structures.
Application method of the present invention:
The first step handles the sperm got, and special frozen solution is added.Second step puts sperm after processing
In the seminal pool 11 for entering slide glass frost slide glass 1, this step needs multiple slide glasses to hold sperm, liquid level be maintained at 11 mouthfuls of seminal pool with
Under.Dilution is added in collecting pit in third step, and liquid level is more than collecting pit 13, then gradually flows into seminal pool 11, this step guarantees essence
Impurity will not enter collecting pit 13 in liquid.4th step places a period of time, and motile sperm can be moved constantly, sperm travelling channel 12
Small in 13 end opening of collecting pit, big in 11 end opening of seminal pool, sperm is easy to swim into collecting pit, is not easy to swim out of.5th step, can be with
The motile sperm gathered taking-up is freezed, it can also be put into liquid nitrogen frozen with slide glass together.
Embodiment 2
The present embodiment and implementation 1 are essentially identical, the difference is that, seminal pool 11, collecting pit 13 in the present embodiment
And sperm travelling channel 12 is detachably connected with frost slide glass.The effect of the way of example is: can directly freezed
In liquid nitrogen, separated sperm can also be transferred in other frost slide glasses and be freezed.
A kind of preferred sperm freezing culture device of the present invention uses simply, utilizes seminal pool, sperm travelling channel, collecting pit
Design can be automatically separated motile sperm, reduce operating quantity, avoid the mechanical damage to sperm of hand picking, improve available
The ratio of motile sperm can replace the frost slide glass of existing all sperms completely, and applicable field has: excellent in normal semen
Selecting sperm to enter, Periodic Traveling IVF, ICSI or artificial insemination, few weak smart patient collects sperm, azoospermia patients operation takes essence, receives
Collect sperm etc..It has broad popularization and application prospect.Slide glass is made using polystyrene material, and intensity is high, non-friable, is had
Good bio-compatibility.There are handle in frost slide glass two sides, facilitate gripping, avoiding accidentally dropping falls, and seminal pool is designed to be open
Big structure, facilitates sperm to swim out of, and collecting pit opening is small, and abdominal cavity is big, and sperm is not easy to swim out of, and improves collection rate.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, under the premise of not departing from the method for the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as
The scope of the present invention.
Claims (10)
1. a kind of preferred sperm freezing culture device, which is characterized in that the freezing culture device is equipped with frost slide glass, sperm
Pond, sperm travelling channel, collecting pit, barrier sheet；The width in the sperm travelling channel is 10-30 microns；The barrier sheet
The slype cooperatively formed with frost slide glass inner surface is sperm travelling channel；Sperm travelling channel connection seminal pool with
Collecting pit.
2. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that sperm travelling channel
And seminal pool, collecting pit inner surface do surface coating processing.
3. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that sperm travelling channel
It is more spacious than connection collecting pit outs open to connect seminal pool part.
4. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that the barrier sheet is fixed on
It freezes on slide glass, is integrally formed structures.
5. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that the frost slide glass uses
Polystyrene material is made.
6. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that seminal pool opening compared with
Greatly, the small shape in abdominal cavity；The collecting pit is small, the big shape in abdominal cavity that is open.
7. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that the appearance of the seminal pool
Product is greater than the volume of collecting pit.
8. a kind of preferred sperm freezing culture device according to claim 7, which is characterized in that the sperm pool volume is
50 to 500 microlitres；The collection pool volume is 5 to 20 microlitres.
9. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that the seminal pool is received
Ji Chi and sperm travelling channel are detachably connected with frost slide glass.
10. a kind of preferred sperm freezing culture device according to claim 1, which is characterized in that the seminal pool is received
Ji Chi and the inner wall in sperm travelling channel are all made of arcuate structure design.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810813651.0A CN109082363A (en) | 2018-07-23 | 2018-07-23 | A kind of preferred sperm freezing culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810813651.0A CN109082363A (en) | 2018-07-23 | 2018-07-23 | A kind of preferred sperm freezing culture device |
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| CN109082363A true CN109082363A (en) | 2018-12-25 |
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| CN201810813651.0A Pending CN109082363A (en) | 2018-07-23 | 2018-07-23 | A kind of preferred sperm freezing culture device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518750A (en) * | 2019-02-01 | 2020-08-11 | 曾繁根 | Sperm sorter and sperm sorting method |
| WO2021018292A1 (en) * | 2019-08-01 | 2021-02-04 | 常州市妇幼保健院 | Method for storing individual sperm or trace amount of sperm, and chip carrier |
| WO2021190644A1 (en) * | 2020-03-27 | 2021-09-30 | 于毅欣 | Method for screening sperm during artificial insemination |
| WO2022160364A1 (en) * | 2021-02-01 | 2022-08-04 | 徐小杨 | Sperm optimization device and operation method therefor |
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| WO2022160364A1 (en) * | 2021-02-01 | 2022-08-04 | 徐小杨 | Sperm optimization device and operation method therefor |
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