CN206204268U - A kind of disc-type chip PCR instrument - Google Patents
A kind of disc-type chip PCR instrument Download PDFInfo
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- CN206204268U CN206204268U CN201621019958.6U CN201621019958U CN206204268U CN 206204268 U CN206204268 U CN 206204268U CN 201621019958 U CN201621019958 U CN 201621019958U CN 206204268 U CN206204268 U CN 206204268U
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Abstract
The utility model discloses a kind of disc-type chip PCR instrument, it includes：Disc-type chip positioning unit, heating unit, high speed centrifugation unit, optical detection unit, low speed turning unit, switch unit and control unit；Disc-type chip positioning unit is positioned to disc-type chip, high speed centrifugation unit drives disc-type chip high-speed rotation, control unit control switch unit switches between high speed centrifugation unit and low speed turning unit, low speed turning unit drives disc-type chip to slowly run, heating unit is heated to disc-type chip simultaneously, now carries out optical detection to detected sample in disc-type chip with optical detection unit.Disc-type chip PCR instrument of the present utility model, high speed centrifugation and low speed fluoroscopic examination are become one, and detection process improves detection efficiency, and reduce the risk in sample handling processes without artificially participating in.
Description
Technical field
The utility model is related to biology and analytical chemistry field, more particularly to a kind of disc-type chip PCR instrument.
Background technology
PCR (PCR) is the important technology of life science, medical domain, for being carried out greatly to target dna
Amount amplification.PCR instrument is the key instrument into performing PCR, is widely used in the cause of disease in diagnosing hereditary illness, detection clinical samples
The nucleic acid of body, legal medical expert's sample is made science of heredity identification etc. aspect.Normal PCR analytical technology is put into sample and carries out using multi-hole orifice
Detection, required Biosample and reagent are more.
Micro-fluidic is, by Micrometer-Nanometer Processing Technology, the sample preparation, separation being related in the fields such as biological and chemical, to mix
The basic operation units such as conjunction, reaction and detection are integrated or are integrated into substantially on one piece of chip of several square centimeters (even more small),
Automation, miniaturization and rapid, laboratory such as sample treatment, sample filtering, the sample point of process can be realized entirely analyzing
From and the whole analysis process integration such as sample detection carry out on one chip.
At present, micro-fluidic chip can be divided into disc type and board-like.For using for disc-type chip sample injection method, be by
After testing sample adds micro-fluidic chip (disc-type chip general designation micro-fluidic chip) sample holes, chip is put into centrifuge at a high speed
Rotation, makes reagent be uniformly distributed into reaction detection pond, also needs for chip to be placed into an other PCR instrument device after being centrifuged enterprising
Row fluorometric investigation.Completing the micro-fluidic PCR detections test of whole constant-temperature amplification need to use two instruments, and chip needs are in two instruments
Between switch, detection process needs manpower to participate in, greatly reduces detection efficiency.Also increase in sample handling processes simultaneously
Risk.
Utility model content
The utility model is directed to above-mentioned problems of the prior art, proposes a kind of disc-type chip PCR instrument, will at a high speed
Centrifugation and low speed fluoroscopic examination become one, and are capable of achieving two automatic switchovers of function, and detection process is carried without artificially participating in
Detection efficiency high, and reduce the risk in sample handling processes.
In order to solve the above technical problems, the utility model is achieved by the following technical solution：
The utility model provides a kind of disc-type chip PCR instrument, and it includes：Disc-type chip positioning unit, heating unit,
High speed centrifugation unit, optical detection unit, low speed turning unit, switch unit and control unit, wherein,
The disc-type chip positioning unit is used to position disc-type chip；
The disc-type chip includes：Sample holes and reaction detection pond, the sample holes are used to inject detected sample；
The high speed centrifugation unit is used to drive the disc-type chip high-speed rotation, the detected sample is uniformly divided
Cloth is in the reaction detection pond；
The heating unit is used to heat the disc-type chip, makes described to be checked in the reaction detection pond
Test sample product are expanded；
The low speed turning unit is used to drive the disc-type chip low speed to turn round；
The optical detection unit is used for when the disc-type chip low speed is turned round, to the institute in the reaction detection pond
Stating detected sample carries out optical detection；
Described control unit is connected with the switch unit, the switch unit and the high speed centrifugation unit and described
Low speed turning unit is connected, and described control unit is used to control the switch unit in the high speed centrifugation unit and the low speed
Switched between turning unit.
It is preferred that the high speed centrifugation unit includes：First motor, output shaft and the disc type of first motor
Chip is connected；
The low speed turning unit includes：Second motor；
The switch unit includes：Switch motor, first gear and second gear, the first gear and described first
The output shaft of motor is connected, and the second gear is connected with the output shaft of second motor, when the high speed centrifugation unit work
When making, the first gear is separated with the second gear, and first motor is used to drive the output shaft of first motor
High-speed rotation, and then drive the disc-type chip high-speed rotation；It is described to cut when needing to be switched to the work of low speed turning unit
Motor is changed for driving the second gear to be meshed with the first gear, second motor is used to drive second electricity
The output shaft rotation of machine, the output shaft of second motor drives the second gear to rotate, and the second gear drives described
First gear is rotated, and the first gear drives the output shaft low speed revolution of first motor, and then drives the disc type
Chip low speed is turned round.
It is preferred that the disc-type chip positioning unit includes：Chip installs detent mechanism and axial-rotation localization machine
Structure.
It is preferred that the chip installs detent mechanism including：Locating dowel and compact heap are constituted, and the locating dowel is fixed on institute
State on the output shaft of high speed centrifugation unit and/or the output shaft of the low speed turning unit, and with the high speed centrifugation unit
The output shaft of output shaft and/or the low speed turning unit is concentric；The compact heap is used to be installed when the disc-type chip
Cheng Shi, compresses to the disc type positioning chip；
The axial-rotation detent mechanism includes：Code-disc and detecting element composition, the code-disc and the high speed centrifugation list
The output shaft of unit is concentric；The detecting element is used to detect the rotation of code-disc, and then determines that the circumference of the disc-type chip is returned
Indexing is put.
It is preferred that also including：Support, the support is used to support the disc-type chip positioning unit, heating unit, height
Fast centrifugal unit, optical detection unit, low speed turning unit, switch unit and control unit.
It is preferred that the heating unit is cavate heating unit；
The cavate heating unit includes：Multiple heating units, multiple heating unit composition cavate heating units.
It is preferred that also including：Shell, the shell is located at the outside of disc-type chip PCR instrument.
Compared to prior art, the utility model has advantages below：
(1) the disc-type chip PCR instrument that the utility model is provided, by high speed centrifugation, amplification and low speed fluorescence detection function
Become one, be capable of achieving to automatically switch, detection process improves detection efficiency, and reduce at sample without artificially participating in
Risk during reason；
(2) disc-type chip PCR instrument of the present utility model, passes through gear between high speed centrifugation unit and low speed turning unit
Engagement is realized automatically switching, and switching is effectively convenient, drives disc-type chip, disc-type chip to position once using same output shaft
, it is simple to operate.
Certainly, implement any product of the present utility model to it is not absolutely required to while reaching all the above advantage.
Brief description of the drawings
Implementation method of the present utility model is described further below in conjunction with the accompanying drawings：
Fig. 1 is the structural representation of the disc-type chip PCR instrument of embodiment of the present utility model；
Fig. 2 is the shell of embodiment of the present utility model and the structural representation of support；
Fig. 3 is the structural representation of the disc-type chip positioning unit of embodiment of the present utility model；
Fig. 4 is the sectional view of the locating dowel of embodiment of the present utility model；
Fig. 5 is the structural representation of the cavate heating unit of embodiment of the present utility model；
Fig. 6 is the schematic diagram of the switch unit of embodiment of the present utility model.
Label declaration：1- disc-type chip positioning units, 2- heating units, 3- high speed centrifugation units, 4- optical detection lists
Unit, 5- low speed turning units, 6- switch units, 7- control units, 8- supports, 9- shells, 10- disc-type chips；
11- chips installation detent mechanism, 12- axial-rotation detent mechanisms,
111- locating dowels, 112- compact heaps, 113- screws；
121- code-discs, 122- detecting elements, 123- nuts；
The heating units of 21- first, the heating units of 22- second；
211- cover plates, 212- heating plates, 213- backplates, 214- bearing pins；
The motors of 31- first；
The output shaft of the motors of 311- first, 312- bearings；
The motors of 51- second；
The motors of 61- the 3rd, 62- first gears, 63- second gears, 64- fixed connecting plates, 65- guide rails；
81- base plates, 82- collateral plates long, the fixed plates of 83- first, 84- short side support plates, the fixed plates of 85- second, 86- pillars,
87- isolates support plate；
91- upper casings, 92- lower casings.
Specific embodiment
Embodiment of the present utility model is elaborated below, the present embodiment is with technical solutions of the utility model as preceding
Put and implemented, give detailed implementation method and specific operating process, but protection domain of the present utility model is not limited
In following embodiments.
With reference to Fig. 1-Fig. 6, disc-type chip PCR instrument of the present utility model is described in detail, its structural representation is such as
Shown in Fig. 1, it includes：Disc-type chip positioning unit 1, heating unit 2, high speed centrifugation unit 3, optical detection unit 4, low speed
Turning unit 5, switch unit 6 and control unit 7.Wherein, disc-type chip positioning unit 1 is used to carry out disc-type chip
Positioning；Disc-type chip includes：Sample holes and reaction detection pond, sample holes are used to inject detected sample；High speed centrifugation list
Unit 3 is used to drive disc-type chip high-speed rotation, detected sample is evenly distributed in reaction detection pond；Heating unit 2 is used
Heated in disc-type chip, expanded the detected sample in reaction detection pond；Low speed turning unit 5 is used to drive
Dynamic disc-type chip low speed revolution；Optical detection unit 4 is used for when disc-type chip low speed is turned round, in reaction detection pond
Detected sample carries out optical detection；Control unit 7 is connected with switch unit 6, switch unit 6 and high speed centrifugation unit 3 and
Low speed turning unit 5 is connected, control unit 7 be used to controlling switch unit 6 high speed centrifugation unit 3 and low speed turning unit 5 it
Between switch over.
The operation principle of the present embodiment is：Detected sample is injected from the sample holes of disc-type chip, high speed centrifugation is used
The driving disc-type chip of unit 3 carries out high-speed rotation, detected sample is carried out centrifugal motion, is evenly distributed to reaction detection pond
In；After detected sample is evenly distributed in reaction detection pond, low speed turning unit is switched to, drives disc-type chip low speed
Revolution, heats when low speed is turned round, while optical detection is carried out to detected sample with optical detection unit, it is to be detected to observe
The amplification phenomenon of sample.
In the present embodiment, high speed centrifugation unit 3 includes：First motor 31, the output shaft 311 and disc type of the first motor 31
Chip is connected, and the first motor 31 carries out high-speed rotation for driving disc-type chip 10；Low speed turning unit 5 includes：Second electricity
Machine 51；Switch unit 6 includes：3rd motor 61, first gear 62 and second gear 63, the motor 31 of first gear 62 and first
Output shaft 311 be connected, second gear 63 is connected with the output shaft of the second motor 51；It is low when being switched to by high speed centrifugation unit 3
During fast turning unit 5, the 3rd motor 61 drives second gear 63 to be meshed with first gear 62, and now the first motor 31 stops work
Make, the second motor 51 drives second gear 63 to rotate, and then drive first gear 62 to rotate, and then drive the defeated of the first motor 31
Shaft 311 is rotated, and then drives the revolution of the low speed of disc-type chip 10.In the present embodiment, in order that switching is more steady, by the 3rd
Motor 61 and second gear 63 are arranged on fixed connecting plate 64, and fixed connecting plate 64 is arranged on guide rail 65, so can be with
Second gear 63 is set more smoothly to be moved along guide rail 65, as shown in Figure 6.
In the present embodiment, in order that disc-type chip positioning is more precisely, disc-type chip positioning unit 1 includes：Chip
Detent mechanism 11 and axial-rotation detent mechanism 12 are installed, as shown in Figure 3.Wherein, chip is installed detent mechanism 11 and is included：It is fixed
Position post 111 and piece 112 is compressed, be both fixed on the output shaft 311 of the first motor 31 by screw 113, and with the
The axle of output 311 of one motor 31 is concentric；Piece is compressed by being arranged on compression of the realization of the spring after compressing piece to disc-type chip,
Compressing piece 112 can stretch, and facilitate being mounted and taken out for disc-type chip.Axial-rotation detent mechanism 12 includes：Code-disc 121 with
And detecting element 122, code-disc 121 is fixed on the output shaft 311 of the first motor 31 by nut 123, and with the first motor
31 output shaft 311 is concentric；Detecting element 122 is arranged in the second fixed plate 85, the rotation of code-disc 121 is detected, for determining
The circumferential rotary position of disc-type chip.
In the present embodiment, for fixation, to fix disc-type chip more steady, the section of locating dowel 111 for two parallel lines with
And the figure that two circular arcs are surrounded, as shown in Figure 4.In different embodiments, the section of locating dowel 111 can also be other polygons
Or irregular figure.
In the present embodiment, in order to disc-type chip positioning unit 1, heating unit 2, high speed centrifugation unit 3, optical detection
Unit 4, low speed turning unit 5, switch unit 6 and control unit 7 are supported well, there is provided support 8, such as Fig. 2 institutes
Show.In the present embodiment, support 8 includes：Base plate 8, the collateral plate 82 of length, the first fixed plate 83, short side support plate 84, the second fixed plate
85th, pillar 86 and isolation support plate 87.Base plate 81 is set and the bottom, and two collateral plates 82 long are vertically arranged at the two of base plate 8
Side, the first fixed plate 83 is set up in the top of the collateral plate 82 of two length, and base plate 81, two grows the collateral shape of 82 and first fixed plate of plate 83
Into a hollow quadrilateral structure, control unit 7 is disposed therein, and first motor page 31 of high speed centrifugation unit is arranged at it
In；Two short side support plates 84 are vertically arranged at the upper end of the first fixed plate 83, and the second fixed plate 85 is set up in two short side support plates 84
Top, the first fixed plate 83, two short side support plates 84 and the second fixed plate also form a hollow structure, low speed turning unit 5
And switch unit 6 is disposed therein；Two pillars 86 are vertically arranged at the upper end of the second fixed plate 85, and isolation support plate 87 is arranged at
The top of two pillars 86, the second fixed plate 85, two pillars 86 and isolation support plate 87 also form a hollow structure, high speed centrifugation
Unit 3 is disposed therein, and the output shaft 311 of the first motor of high speed centrifugation unit 3 sequentially passes through the first fixed plate 83 and
Two fixed plates 85 are connected with disc-type chip 10, and the output shaft 311 of the first motor is fixed on the second fixed plate by bearing 312
In 85, the stabilization rotation of the first motor 31 is realized；Optical detection unit 4 is arranged at the upper end of the second fixed supported slab 85；Disk
Formula chip positioning unit and disc-type chip are arranged at the upper end of isolation support plate 87.The supporting structure of the present embodiment is reasonable in design,
Both 5 space is saved, whole PCR instrument Stability Analysis of Structures can be made again, but be not herein that, to limitation of the present utility model, difference is real
In applying example, support can have a different designs, and each unit is also dependent on the arrangement for needing to carry out diverse location.
In the present embodiment, in order to be protected to whole PCR instrument, it is disturbed by external environment less, be additionally provided with outer
Shell 9, shell 9 is located at the outside of all units.And the heating unit 2 in the present embodiment is non-contact thermal, including two
Heating unit：First heating unit 21 and the second heating unit 22, the first heating unit 21 are arranged on the top of shell 9, first
Heating unit 21 includes：Three layers for setting gradually, respectively：Cover plate 211, heating plate 212 and backplate 213, the first heating are single
Unit 21 is fixed on shell 9 by bearing pin 214, can bearing pin 214 rotate, opened or closed with realization, as shown in Figure 5.Work as needs
When installing fixed disc formula chip, the first heating unit 21 is opened, and after installation, the first heating unit 21 closes；
After closure, the first heating unit 21 and the second heating unit being arranged on support 8 constitute heating chamber, and disc-type chip is carried out
Heating, so that the detected sample amplification in the reaction tank of disc-type chip, the special heating unit of the present embodiment is to disk
The heating effect of formula chip, can better ensure that the uniformity of the detected sample temperature in disc-type chip.Difference is implemented
In example, heating unit can be arranged on different positions, and the quantity of heating unit also differs and is set to two, as long as can be to disk
Formula heating unit is heated, and expands it.
In the present embodiment, remove and install for convenience, 9 points of shell is upper and lower two parts, respectively：Upper casing 91, lower casing
92.In different embodiments, shell 9 can also be integrated, or by two parts composition described above.
In preferred embodiment, uncapped state detection means is additionally provided with the first heating unit 21 and shell 9, for realizing
Uncapped state is detected.
In preferred embodiment, when control unit 7 is additionally operable to realize that low speed is turned round, the positioning of reaction detection pond position and inspection
The functions such as the related operation of survey, computer heating control, temperature detection, uncapped state detection and optical detection.
Disclosed herein is only preferred embodiment of the present utility model, and this specification is chosen and specifically describes these implementations
Example, it, in order to preferably explain principle of the present utility model and practical application, is not to restriction of the present utility model to be.Any
The modifications and variations that art personnel are done in the range of specification, all should fall in the range of the utility model is protected.
Claims (7)
1. a kind of disc-type chip PCR instrument, it is characterised in that including：Disc-type chip positioning unit, heating unit, at a high speed from
Heart unit, optical detection unit, low speed turning unit, switch unit and control unit, wherein,
The disc-type chip positioning unit is used to position disc-type chip；
The disc-type chip includes：Sample holes and reaction detection pond, the sample holes are used to inject detected sample；
The high speed centrifugation unit is used to drive the disc-type chip high-speed rotation, is evenly distributed to the detected sample
In the reaction detection pond；
The heating unit is used to heat the disc-type chip, makes the test sample described to be checked in the reaction detection pond
Product are expanded；
The low speed turning unit is used to drive the disc-type chip low speed to turn round；
The optical detection unit is used for when the disc-type chip low speed is turned round, to treating described in the reaction detection pond
Detection sample carries out optical detection；
Described control unit is connected with the switch unit, the switch unit and the high speed centrifugation unit and the low speed
Turning unit is connected, and described control unit is used to control the switch unit to be turned round in the high speed centrifugation unit and the low speed
Switched between unit.
2. disc-type chip PCR instrument according to claim 1, it is characterised in that the high speed centrifugation unit includes：First
Motor, the output shaft of first motor is connected with the disc-type chip；
The low speed turning unit includes：Second motor；
The switch unit includes：Switch motor, first gear and second gear, the first gear and first motor
Output shaft be connected, the second gear is connected with the output shaft of second motor, when the high speed centrifugation cell operation,
The first gear is separated with the second gear, and first motor is used to drive the output shaft of first motor to turn at a high speed
It is dynamic, and then drive the disc-type chip high-speed rotation；When needing to be switched to the work of low speed turning unit, the switch motor
For driving the second gear to be meshed with the first gear, second motor is used to drive the defeated of second motor
Shaft is rotated, and the output shaft of second motor drives the second gear to rotate, and the second gear drives first tooth
Wheel is rotated, and the first gear drives the output shaft low speed revolution of first motor, and then drives the disc-type chip low
Speed revolution.
3. disc-type chip PCR instrument according to claim 1, it is characterised in that the disc-type chip positioning unit bag
Include：Chip installs detent mechanism and axial-rotation detent mechanism.
4. disc-type chip PCR instrument according to claim 3, it is characterised in that the chip installs detent mechanism to be included：
Locating dowel and compact heap are constituted, and the locating dowel is fixed on output shaft and/or the low speed revolution of the high speed centrifugation unit
On the output shaft of unit, and output shaft and/or the output shaft of the low speed turning unit with the high speed centrifugation unit is concentric；
The compact heap is used to, when the disc-type chip installation, compress the disc type positioning chip；
The axial-rotation detent mechanism includes：Code-disc and detecting element composition, the code-disc and the high speed centrifugation unit
Output shaft is concentric；The detecting element is used to detect the rotation of code-disc, and then determines the circumferential revolution position of the disc-type chip
Put.
5. disc-type chip PCR instrument according to claim 1, it is characterised in that also include：Support, the support is used for
Support the disc-type chip positioning unit, heating unit, high speed centrifugation unit, optical detection unit, low speed turning unit, cut
Change unit and control unit.
6. disc-type chip PCR instrument according to claim 1, it is characterised in that it is single that the heating unit is that cavate is heated
Unit；
The cavate heating unit includes：Multiple heating units, multiple heating unit composition cavate heating units.
7. disc-type chip PCR instrument according to claim 1, it is characterised in that also include：Shell, the jacket sets
In the outside of disc-type chip PCR instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621019958.6U CN206204268U (en) | 2016-08-31 | 2016-08-31 | A kind of disc-type chip PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621019958.6U CN206204268U (en) | 2016-08-31 | 2016-08-31 | A kind of disc-type chip PCR instrument |
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| Publication Number | Publication Date |
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| CN206204268U true CN206204268U (en) | 2017-05-31 |
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ID=58760648
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| CN201621019958.6U Active CN206204268U (en) | 2016-08-31 | 2016-08-31 | A kind of disc-type chip PCR instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106222069A (en) * | 2016-08-31 | 2016-12-14 | 上海科源电子科技有限公司 | A kind of disc-type chip PCR instrument and detection method |
| CN112547141A (en) * | 2019-09-25 | 2021-03-26 | 深圳市刚竹医疗科技有限公司 | Coded disc structure for microfluidic positioning and microfluidic analysis device |
-
2016
- 2016-08-31 CN CN201621019958.6U patent/CN206204268U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106222069A (en) * | 2016-08-31 | 2016-12-14 | 上海科源电子科技有限公司 | A kind of disc-type chip PCR instrument and detection method |
| CN106222069B (en) * | 2016-08-31 | 2018-11-09 | 上海科源电子科技有限公司 | A kind of disc-type chip PCR instrument and detection method |
| CN112547141A (en) * | 2019-09-25 | 2021-03-26 | 深圳市刚竹医疗科技有限公司 | Coded disc structure for microfluidic positioning and microfluidic analysis device |
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