CN206204267U - Kit of the one kind for mammalian cell " gene editing " vector construction - Google Patents
Kit of the one kind for mammalian cell " gene editing " vector construction Download PDFInfo
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- CN206204267U CN206204267U CN201620356685.8U CN201620356685U CN206204267U CN 206204267 U CN206204267 U CN 206204267U CN 201620356685 U CN201620356685 U CN 201620356685U CN 206204267 U CN206204267 U CN 206204267U
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- gene editing
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Abstract
The utility model discloses the kit that one kind is used for mammalian cell " gene editing " vector construction, including box body, pad is fixed with box body, container hole is fixed with pad, EP pipes are fixed with container hole.The gene editing kit that the utility model is provided cause to carry out mammalian genes gene editing become to be more prone to efficiently.User only needs to, according to genes of interest design synthesis SgRNA sequences, the carrier of expression Cas9 enzymes and SgRNA can be obtained after simple clone, can carry out fixed point gene editing to genes of interest.
Description
Technical field
The utility model belongs to biological technical field, and in particular to one kind is used for mammalian cell " gene editing " carrier
The kit of structure.
Background technology
Gene editing (Gene Editing) technology is that organism genome is carried out with RNA nucleotide sequences using nuclease
The technology of shearing, including the first generation ZFN zinc finger zymotechnics, the TALEN technologies of the second generation, and the third generation CRISPR Cas
Technology.The CRISPR Cas technologies of the current third generation have become the mainstream technology of gene editing.
CRISPR Cas are the important components of bacterium (Bacteria) and immune system in archeobacteria (archaea),
It is main to include the Cas9 albumen with DNA enzymatic activity, and CrRNA and TracrRNA, can combine and shear external source bacteriophage
DNA.Using the Cas9 enzymes crossed through sequence optimisation and SgRNA (have concurrently CrRNA with TracrRNA functions) was transformed, can be right
Gene knockout or gene knock-in that the genome of mammalian cell or embryonated egg is pinpointed (donor need to be provided during gene knock-in).With
The conventional method using homologous recombination (homologous recombination) carries out modification phase to mammalian genome
Than, CRISPR Cas technologies more quickly with efficiently, therefore build in gene functional research, disease model, disease gene treatment,
Biological genetic breeding etc. is all many-sided with important application value.
Utility model content
The technical problems to be solved in the utility model is to overcome existing defect, there is provided one kind is based on CRISPR Cas technologies
High efficient and reliable, mammalian cell gene editor's vector construction kit easy to use.
In order to solve the above-mentioned technical problem, the utility model provides following technical scheme：
A kind of kit for mammalian cell " gene editing " vector construction of the utility model, including box body, institute
State and be fixed with box body pad, container hole is fixed with the pad, EP pipes, three institutes are fixed with the container hole
State in EP pipes and fix respectively equipped with PHMG-SgRNA linearization plasmids, PHMG-SgRNA sequencing primers and PHMG-SgRNA-Tet
With reference to plasmid.
Used as a kind of optimal technical scheme of the present utility model, the both sides of the box body are fixed with handle.
Used as a kind of optimal technical scheme of the present utility model, the container hole is fixedly installed three, and the container
Hole is evenly arranged on the pad.
The beneficial effect that the utility model is reached is：The gene editing kit that the utility model is provided is caused to lactation
Animal gene carry out gene editing become to be more prone to efficiently.User is only needed to according to genes of interest design synthesis SgRNA sequences
Row, can obtain the carrier of expression Cas9 enzymes and SgRNA after simple clone, fixed point gene can be carried out to genes of interest and is compiled
Volume.
Brief description of the drawings
Accompanying drawing is used for providing being further understood to of the present utility model, and constitutes a part for specification, with this practicality
New embodiment is used to explain the utility model together, does not constitute to limitation of the present utility model.In the accompanying drawings：
Fig. 1 is subjective structural representation of the present utility model；
In figure：1st, box body；2nd, pad；3rd, container hole；4th, EP pipes；5th, handle.
Specific embodiment
Preferred embodiment of the present utility model is illustrated below in conjunction with accompanying drawing, it will be appreciated that described herein excellent
Select embodiment to be merely to illustrate and explain the utility model, be not used to limit the utility model.
Embodiment 1
As shown in Figure 1, the utility model provides a kind of reagent for mammalian cell " gene editing " vector construction
Pad 2 is fixed with box, including box body 1, box body 1, is fixed with pad 2 in container hole 3, container hole 3 and is fixed with EP
Pipe 4.
Further, the both sides of box body 1 are fixed with handle 5, convenient to guide and support kit by handle, are convenient for carrying, together
When be easy in handling process keep EP pipes balance, convenient use.
Container hole 3 is fixedly installed three, and container hole 3 is evenly arranged on pad 2, facilitates picking and placeing for EP pipes 4, is easy to
Use.
It is fixed respectively in three EP pipes 4 that PHMG-SgRNA linearization plasmids, PHMG-SgRNA sequencing primers and PHMG- are housed
The reference plasmid of SgRNA-Tet1, conveniently carries out gene editing, is easy to use.
The gene editing kit that the utility model is provided causes that carrying out gene editing to mammalian genes becomes more
Easily with efficiently.User only needs to, according to genes of interest design synthesis SgRNA sequences, to be expressed after simple clone
Cas9 enzymes and the carrier of SgRNA, can carry out fixed point gene editing to genes of interest.
Finally it should be noted that：Preferred embodiment of the present utility model is the foregoing is only, this is not limited to
Utility model, although being described in detail to the utility model with reference to the foregoing embodiments, for those skilled in the art
For, it can still modify to the technical scheme described in foregoing embodiments, or to which part technical characteristic
Carry out equivalent.It is all it is of the present utility model spirit and principle within, any modification, equivalent substitution and improvements made etc.,
Should be included within protection domain of the present utility model.
Claims (3)
1. a kind of kit for mammalian cell " gene editing " vector construction, including box body (1), it is characterised in that
Pad (2) is fixed with the box body (1), container hole (3) is fixed with the pad (2), it is solid in the container hole (3)
Surely EP pipes (4) is provided with, it is fixed respectively in three EP pipes (4) to be surveyed equipped with PHMG-SgRNA linearization plasmids, PHMG-SgRNA
The reference plasmid of sequence primer and PHMG-SgRNA-Tet1.
2. one kind according to claim 1 is for the kit of mammalian cell " gene editing " vector construction, and it is special
Levy and be, the both sides of the box body (1) are fixed with handle (5).
3. one kind according to claim 1 is for the kit of mammalian cell " gene editing " vector construction, and it is special
Levy and be, the container hole (3) is fixedly installed three, and the container hole (3) is evenly arranged on the pad (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620356685.8U CN206204267U (en) | 2016-04-26 | 2016-04-26 | Kit of the one kind for mammalian cell " gene editing " vector construction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620356685.8U CN206204267U (en) | 2016-04-26 | 2016-04-26 | Kit of the one kind for mammalian cell " gene editing " vector construction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206204267U true CN206204267U (en) | 2017-05-31 |
Family
ID=58755149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620356685.8U Expired - Fee Related CN206204267U (en) | 2016-04-26 | 2016-04-26 | Kit of the one kind for mammalian cell " gene editing " vector construction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206204267U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728330A (en) * | 2018-06-19 | 2018-11-02 | 东台德缘生物科技有限公司 | A kind of kit for mammalian cell gene editor's vector construction |
-
2016
- 2016-04-26 CN CN201620356685.8U patent/CN206204267U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728330A (en) * | 2018-06-19 | 2018-11-02 | 东台德缘生物科技有限公司 | A kind of kit for mammalian cell gene editor's vector construction |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170531 Termination date: 20180426 |
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| CF01 | Termination of patent right due to non-payment of annual fee |