CN206177956U - Lactate dehydrogenase toxicity detection kit - Google Patents
Lactate dehydrogenase toxicity detection kit Download PDFInfo
- Publication number
- CN206177956U CN206177956U CN201621227101.3U CN201621227101U CN206177956U CN 206177956 U CN206177956 U CN 206177956U CN 201621227101 U CN201621227101 U CN 201621227101U CN 206177956 U CN206177956 U CN 206177956U
- Authority
- CN
- China
- Prior art keywords
- hole
- cell culture
- test tube
- box
- lactic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a lactate dehydrogenase toxicity detection kit, including holding the box, hold the box the inner wall on fixed mounting have the baffle, the through -hole has been seted up on the surface of baffle, fixed mounting has clamping device in the through -hole, hold the box the inner wall on fixed mounting have the cardboard, the cardboard is located the below of baffle, and corresponds on the cardboard and seted up the card hole on the position of through -hole, the inner chamber bottom fixed mounting in card hole has retaining snap ring. The utility model discloses a setting holds box, baffle, through -hole, clamping device, cardboard, card hole and retaining snap ring's cooperation, prevents that the cell culture test tube from rocking in holding the box, blocks up test tube stopper through setting up test tube stopper, pipe and suction nozzle, prevents to hold the box and rocks and arouse rocking of cell culture test tube to the cell liquid that leads to the cell culture in vitro flows, has reached to prevent to lead to the cell liquid in the cell culture board to spill the effect because of the shake.
Description
Technical field
This utility model is related to test kit technical field, specially a kind of lactic acid dehydrogenase toxicity detection test kit.
Background technology
Lactic acid dehydrogenase citotoxicity detection kit (LDH Cytotoxicity Assay Kit), also referred to as lactic dehydrogenase
Enzyme detection kit (LDH Assay Kit) or lactic dehydrogenase enzyme r e lease detection kit (LDH Release Assay Kit),
Be it is a kind of based on diaphorase be catalyzed INT chromogenic reactions, the lactic dehydrogenase discharged during by colorimetric determination cytotoxicity
Enzymatic activity detects the test kit of lactic acid dehydrogenase activity in other samples.
But lactic acid dehydrogenase citotoxicity detection kit in the market is when in use, needs are first inoculated with cell
To in Tissue Culture Plate, then place in cell culture incubator and be incubated, take out cell plates after the completion of culture, Jing during this
The shake because handss is often susceptible to, is caused Tissue Culture Plate to rock the Cell sap for making it internal and is spilt, so as to affect what is tested
Accuracy.
Utility model content
The purpose of this utility model is to provide a kind of lactic acid dehydrogenase toxicity detection test kit, and possessing can clamp culture
The advantage of test tube, solving the lactic acid dehydrogenase citotoxicity detection kit in the market mentioned in background technology makes
Used time, need first to seed cells in Tissue Culture Plate, then place in cell culture incubator and be incubated, will be thin after the completion of culture
Born of the same parents' plate takes out, and Jing is often susceptible to the shake because handss during this, causes Tissue Culture Plate to rock the cell for making it internal
Liquid spills, so as to affect the problem of the accuracy tested.
For achieving the above object, this utility model provides following technical scheme：A kind of lactic acid dehydrogenase toxicity detection reagent
Box, including holding box, are installed with baffle plate on the inwall of the holding box, the surface of the baffle plate offers through hole, described
Clamping device is installed with through hole, clamp is installed with the inwall of the holding box, the clamp is located at baffle plate
Hole clipping is offered on the position of correspondence through hole on lower section, and clamp, the intracavity bottom of the hole clipping is installed with snap ring,
Tissue Culture Plate is overlapped with the top of the baffle plate, the surface of the Tissue Culture Plate is plugged with cell culture tube, described thin
Test tube plug is installed at the top of born of the same parents' culture test tube, and the bottom of cell culture tube is through snap ring and extends to the interior of hole clipping
Chamber, is plugged with the conduit of connection cell culture tube and extraneous air at the axle center of the test tube plug.
The clamping device includes clamping ring, and fixed sleeving, the fixed sleeving are inlaid with the inwall of the clamping ring
Inner chamber in be installed with spring, one end of the spring is fixedly connected with one end of mobile bar, one end of the mobile bar
Fixed sleeving is passed, and is fixedly connected with the outer surface of India rubber circle.
Preferably, cover plate is connected with the top of the holding box, and the bottom of cover plate is installed with limited block.
Preferably, one end of the conduit passes cover plate and is fixedly connected with the bottom of suction nozzle.
Preferably, the quantity of the through hole and hole clipping is 96.
Preferably, the quantity of the fixed sleeving is 384, and four fixed sleevings are one group with 90 degree
It is angle annular array between clamping ring and India rubber circle.
Compared with prior art, the beneficial effects of the utility model are as follows：
1st, this utility model is by arranging matching somebody with somebody for holding box, baffle plate, through hole, clamping device, clamp, hole clipping and snap ring
Close, cell culture tube is fixedly mounted in holding box, cell culture tube is passed through into the through hole of baffle plate, and insert clamp
In hole clipping, so as to cell culture tube be fixed, by the cooperation of clamping device and snap ring, to cell culture tube
Position be fixed, prevent cell culture tube from rocking in holding box, by arranging test tube plug, conduit and suction nozzle to examination
Pipe is blocked, while the oxygen for alloing outside enters test tube interior by suction nozzle, for cell oxygen is provided, and prevents from holding
Box is rocked and causes rocking for cell culture tube, and so as to cause the Cell sap in cell culture tube to flow out, having reached prevents
The Cell sap in Tissue Culture Plate is caused to spill effect because of shake.
Description of the drawings
Fig. 1 is this utility model structural representation；
Fig. 2 is this utility model A portions enlarged drawing；
Fig. 3 is this utility model conduit and suction nozzle connection diagram.
In figure：1 holding box, 2 baffle plates, 3 through holes, 4 clamping devices, 401 clamping rings, 402 fixed sleevings, 403 springs, 404
Mobile bar, 405 India rubber circles, 5 clamps, 6 hole clippings, 7 snap rings, 8 Tissue Culture Plates, 9 cell culture tubes, 10 test tubes
Plug, 11 conduits, 12 cover plates, 13 limited blocks, 14 suction nozzles.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment for being obtained, belongs to the scope of this utility model protection.
Fig. 1-3, a kind of lactic acid dehydrogenase toxicity detection test kit, including holding box 1 are referred to, holding box 1 is used to place
Tissue Culture Plate 8, makes the taking and placing enhanced convenience of Tissue Culture Plate 8, it is therefore prevented that because hand shaking causes during taking and placing Tissue Culture Plate 8
Cell sap in Tissue Culture Plate 8 spills, and the top of holding box 1 is connected with cover plate 12, and the bottom of cover plate 12 is installed with
Limited block 13, is installed with baffle plate 2 on the inwall of holding box 1, the surface of baffle plate 2 offers through hole 3, fixed peace in through hole 3
Equipped with clamping device 4, clamp 5 is installed with the inwall of holding box 1, clamp 5 is located at the lower section of baffle plate 2, and right on clamp 5
The quantity that hole clipping 6, through hole 3 and hole clipping 6 are offered on the position for answering through hole 3 is 96, and the intracavity bottom of hole clipping 6 is fixed
Snap ring 7 is installed, the top of baffle plate 2 is overlapped with Tissue Culture Plate 8, by the cooperation of cover plate 12 and limited block 13 by cell
Culture plate 8 is fixed between limited block 13 and baffle plate 2, and Tissue Culture Plate 8 is fixed, and prevents rocking for Tissue Culture Plate 8,
The surface of Tissue Culture Plate 8 is plugged with cell culture tube 9, and the quantity of cell culture tube 9 is 90 six roots of sensation, by cell culture
In through hole 3 on the insertion baffle plate 2 of test tube 9, and insert in the hole clipping 6 on clamp 5, so that the position of cell culture tube 9 is entered
Row is fixed, and cell culture tube 9 is fixed by clamping device 4 and snap ring 7, prevents cell culture tube 9 from occurring
Rock, the top of cell culture tube 9 is provided with test tube plug 10, and connection cell culture tube is plugged with the axle center of test tube plug 10
9 and the conduit 11 of extraneous air, and the bottom of cell culture tube 9 is through snap ring 7 and extends to the inner chamber of hole clipping 6, leads
One end of pipe 11 passes cover plate 12 and is fixedly connected with the bottom of suction nozzle 14, the Cell sap placement amount in cell culture tube 9
Maximum is less than 1/2nd of the capacity of cell culture tube 9, by arranging test tube plug 10, conduit 11 and suction nozzle 14 to cell
Culture test tube 9 is blocked, while the oxygen for alloing outside is entered inside cell culture tube 14 by suction nozzle 14, it is thin
Born of the same parents provide oxygen, prevent holding box 1 from rocking and causing rocking for cell culture tube 9, so as to cause in cell culture tube 9
Cell sap flows out.
Clamping device 4 includes clamping ring 401, and fixed sleeving 402, fixed sleeving 402 are inlaid with the inwall of clamping ring 401
Inner chamber in be installed with spring 403, one end of spring 403 is fixedly connected with one end of mobile bar 404, mobile bar 404
One end passes fixed sleeving 402, and is fixedly connected with the outer surface of India rubber circle 405, and the quantity of fixed sleeving 402 is 300
84, and it is angle annular array in clamping ring 401 and India rubber circle that four fixed sleevings 402 are one group with 90 degree
Between 405, India rubber circle 405 can be strutted when cell culture tube 9 passes through clamping device 4, while extrude mobile bar 404 pressing
Spring in contracting fixed sleeving 402, by using spring 403 restoring force and India rubber circle 405 shrink elasticity to carrying out
Clamp, so as to reach the effect of immobilized-cell culture test tube 9.
When using, Tissue Culture Plate 8 is placed on into the top of baffle plate 2, then cell culture tube 9 is passed through into cell culture
The through hole 3 of plate 8 and baffle plate 2, and insert in the hole clipping 6 of clamp 5, so as to cell culture tube 9 be fixed, by clamping dress
The cooperation of 4 and snap ring 7 is put, the position of cell culture tube 9 is fixed, prevent cell culture tube 9 in holding box 1
Inside rock, after cell culture fluid and cell are put in cell culture tube 9, test tube plug 10 and cover cover plate 12 beyond the Great Wall, pass through
Limited block 13 makes Tissue Culture Plate 8 be fixed between baffle plate 2 and limited block 13, while by the cooperation of conduit 11 and suction nozzle,
The air for alloing outside is entered in cell culture tube 9, and the Cell sap in cell culture tube 9 cannot flow out.
It should be noted that term " including ", "comprising" or its any other variant are intended to the bag of nonexcludability
Contain, so that a series of process, method, article or equipment including key elements is not only including those key elements, but also including
Other key elements being not expressly set out, or also include the key element intrinsic for this process, method, article or equipment.
In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that including the key element
Process, method, article or equipment in also there is other identical element.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out various changes in the case of without departing from principle of the present utility model and spirit, repaiies
Change, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of lactic acid dehydrogenase toxicity detection test kit, including holding box (1), it is characterised in that：The holding box (1) it is interior
Baffle plate (2) is installed with wall, the surface of the baffle plate (2) offers and is installed with through hole (3), the through hole (3)
Clamping device (4), is installed with clamp (5) on the inwall of the holding box (1), the clamp (5) is under baffle plate (2)
Side, and hole clipping (6) is offered on the position of correspondence through hole (3) on clamp (5), it is installed with the inwall of the hole clipping (6)
Snap ring (7), is overlapped with Tissue Culture Plate (8), the surface grafting of the Tissue Culture Plate (8) at the top of the baffle plate (2)
There is cell culture tube (9), test tube plug (10), and cell culture tube (9) are installed at the top of the cell culture tube (9)
Bottom is through snap ring (7) and extends to the inner chamber of hole clipping (6), connection is plugged with the axle center of the test tube plug (10) thin
The conduit (11) of born of the same parents' culture test tube (9) and extraneous air；
The clamping device (4) includes clamping ring (401), and on the inwall of the clamping ring (401) fixed sleeving is inlaid with
(402), spring (403), one end of the spring (403) and movement are installed with the inner chamber of the fixed sleeving (402)
One end of bar (404) is fixedly connected, and one end of the mobile bar (404) passes fixed sleeving (402), and with India rubber circle
(405) outer surface is fixedly connected.
2. a kind of lactic acid dehydrogenase toxicity detection test kit according to claim 1, it is characterised in that：The holding box
(1) cover plate (12) is connected with the top of, and the bottom of cover plate (12) is installed with limited block (13).
3. a kind of lactic acid dehydrogenase toxicity detection test kit according to claim 1 and 2, it is characterised in that：The conduit
(11) one end passes cover plate (12) and is fixedly connected with the bottom of suction nozzle (14).
4. a kind of lactic acid dehydrogenase toxicity detection test kit according to claim 1, it is characterised in that：The through hole (3)
96 are with the quantity of hole clipping (6).
5. a kind of lactic acid dehydrogenase toxicity detection test kit according to claim 1, it is characterised in that：The fixed sleeving
(402) quantity is 384, and four fixed sleevings (402) are that angle annular array is being pressed from both sides with 90 degree for one group
Tightly between ring (401) and India rubber circle (405).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621227101.3U CN206177956U (en) | 2016-11-11 | 2016-11-11 | Lactate dehydrogenase toxicity detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621227101.3U CN206177956U (en) | 2016-11-11 | 2016-11-11 | Lactate dehydrogenase toxicity detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206177956U true CN206177956U (en) | 2017-05-17 |
Family
ID=58684859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621227101.3U Expired - Fee Related CN206177956U (en) | 2016-11-11 | 2016-11-11 | Lactate dehydrogenase toxicity detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206177956U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596819A (en) * | 2019-01-11 | 2019-04-09 | 河南中医药大学 | A kind of novel nephrosis detection kit |
| CN114130449A (en) * | 2021-11-19 | 2022-03-04 | 陕西理工大学 | Kit for detecting resistance allele of abortion disease of milk goats |
| CN115074235A (en) * | 2022-06-30 | 2022-09-20 | 深圳源兴基因技术有限公司 | Stable culture device for vector gene vaccine |
-
2016
- 2016-11-11 CN CN201621227101.3U patent/CN206177956U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109596819A (en) * | 2019-01-11 | 2019-04-09 | 河南中医药大学 | A kind of novel nephrosis detection kit |
| CN109596819B (en) * | 2019-01-11 | 2021-12-17 | 河南中医药大学 | Novel kidney disease detection kit |
| CN114130449A (en) * | 2021-11-19 | 2022-03-04 | 陕西理工大学 | Kit for detecting resistance allele of abortion disease of milk goats |
| CN115074235A (en) * | 2022-06-30 | 2022-09-20 | 深圳源兴基因技术有限公司 | Stable culture device for vector gene vaccine |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170517 Termination date: 20181111 |