CN206082684U - A low temperature needle grinds for preparing cell culture base - Google Patents
A low temperature needle grinds for preparing cell culture base Download PDFInfo
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- CN206082684U CN206082684U CN201621039281.2U CN201621039281U CN206082684U CN 206082684 U CN206082684 U CN 206082684U CN 201621039281 U CN201621039281 U CN 201621039281U CN 206082684 U CN206082684 U CN 206082684U
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- abrasive material
- low temperature
- shower nozzle
- material storehouse
- grinds
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Abstract
The utility model provides a low temperature needle grinds for preparing cell culture base, belongs to the edge -runner mill field for solve in the cell culture base production process problem to microthermal requirement. The utility model discloses comprise casing, feed inlet, quiet dish, driving disk, discharge gate, motor and transmission system, wherein the space between quiet dish and the driving disk constitutes the abrasive material storehouse, and there is an one or more coolant liquid shower nozzle in central authorities in the abrasive material storehouse, and the shower nozzle can erupt chemical property to the abrasive material storehouse down in the pressure effect can quick pneumatolytic coolant liquid for the inertia, like the liquid nitrogen, to carrying out cooling in whole abrasive material storehouse, there is the temperature detect meter near the material contact surface on quiet dish. The utility model discloses a cooling provision is more direct to the centrifugal force that has utilized the needle to grind promotes air conditioning in abrasive material storehouse in -diffusion, and cooling speed is faster, the effect is better, can also intelligent control, the temperature in record abrasive material storehouse, and validity, security and the stability of the basic composition of cell culture guaranteeing prepare.
Description
Technical field
This utility model belongs to mill, more particularly to a kind of low temperature pin for preparing cell culture medium grinds.
Background technology
Biomedicine field is one of domestic and international fastest-rising industry, and mammalian cell expression system becomes antibody medicine
It is quite varied Deng application of the mammalian cells such as the main flow expression system of bio-pharmaceuticals, CHO, NS0 on protein expression, at present
Kilolitre, ten thousand upgradings have been reached in the culture scale of some macro-organism pharmaceutical factory mammalian cells.
The mammalian cell that biomedicine field is used is the cell of isolated culture, either to the absorption of nutrient substance
Ability is all very crisp compared with the microbial expression host cell such as antibacterial, yeast still to the resistivity of poisonous and harmful substance
It is weak.Cell culture medium is isolated cells existence and the environment bred, by several amino acids, sugar, vitamin, trace element and albumen
Matter etc. is into being grouped into.The Multiple components of cell culture medium be heated easily decompose or aoxidize, it is rotten, so temperature in process of production
Control is particularly significant, preferably carries out in a low temperature of less than 25 DEG C.
One necessary links of cell culture medium production process are crushing materials, are traditionally used mostly ball mill.But
Ball mill is bulky, production capacity is low, it is impossible to meet the demand of large-scale production.Needle mill by adjacently situated surfaces have vertical needle bar
Two horizontal steel disks compositions, a disk（Quiet disk）Maintain static, and another disk（Moving plate）Make to rotate at a high speed, material is from upper disk
Central authorities addition, thrown under the action of the centrifugal force outside and occur clash into, crush.Pin grinding tool has high excellent of small volume, production capacity
Point, is used widely in agricultural and field of food.But needle mill has the shortcomings that quantity of heat production is big, it is slow to radiate, and is it
Production for cell culture medium causes a difficult problem.
The content of the invention
For the requirement in cell culture medium production process to low temperature, this utility model provides a kind of for preparing cell
The low temperature pin mill of culture medium, technical scheme is as follows：
A kind of low temperature pin for preparing cell culture medium grinds, by housing（1）, charging aperture（2）, quiet disk（3）, Moving plate（4）、
Discharging opening（5）, motor（6）And drive system（7）Composition, wherein the space between quiet disk and Moving plate constitutes abrasive material storehouse, in abrasive material
There are one or more coolant shower nozzles in the central authorities in storehouse（8）, shower nozzle and cooling liquid container（9）It is connected by pipeline, shower nozzle can be in pressure
Power effect under to abrasive material storehouse eject chemical property for inertia and can Quick-gasifying coolant.The effect of centrifugal force is ground in pin
Under, the cold air in central authorities can rapidly diffuse into abrasive material storehouse outer rim, and whole abrasive material storehouse is cooled down.
Preferably, described quiet disk is located at the top of Moving plate, and described coolant shower nozzle is located on quiet disk, as shown in Figure 1.
Preferably, the quantity of described coolant shower nozzle is 2 to 10, is distributed in the both sides of the enterprising material mouth lower end of quiet disk,
Or it is annular in shape（Radiation symmetric）Equably it is arranged in around charging aperture lower end.
Preferably, there is cooling sandwith layer between the outer wall and inwall of housing（10）, filled with coolant in interlayer, such as Fig. 2 institutes
Show.
Preferably, there is temperature sensing meter near material contact surface on quiet disk（11）, outside temperature sensing meter and pin mill
Computer or LCDs, alarm, monitor are carried out data transmission by data wire or radio wave, so as to realize to mill
The monitoring of feed bin temperature, warning, record.
Preferably, described coolant shower nozzle is by independent motor（12）And drive system（13）Unified driving is controlled, and is driven
The motor of dynamic shower nozzle is connected with computer, can pass through the ejection frequency of computer software control shower nozzle, combination temperature surveymeter
Monitoring Data, is capable of achieving the intelligent control to abrasive material storehouse temperature.
Above-described coolant is liquid nitrogen.
The beneficial effects of the utility model are, compared with traditional outside cooling method, cooling of the present utility model is arranged
Apply more directly, and make use of the centrifugal force that pin grinds to promote cold air in abrasive material storehouse internal diffusion, cooling rate faster, effect more preferably, and
Can Based Intelligent Control, the temperature in record abrasive material storehouse, it is ensured that the effectiveness of prepared cell culture media component, safety and stable
Property.
Description of the drawings
Fig. 1：The sectional side elevation that a kind of low temperature pin for preparing cell culture medium grinds；
Fig. 2：The sectional side elevation that a kind of low temperature pin for preparing cell culture medium with cooling sandwith layer grinds.
Specific embodiment
Embodiment 1, a kind of low temperature pin for preparing cell culture medium grind
A kind of low temperature pin for preparing cell culture medium grinds, as shown in figure 1, by housing（1）, charging aperture（2）, quiet disk
（3）, Moving plate（4）, discharging opening（5）, mair motor（6）And drive system（7）Composition, wherein quiet disk is located at the top of Moving plate, quiet disk
Space between Moving plate constitutes abrasive material storehouse, has 2 coolant shower nozzles in the central authorities in abrasive material storehouse（8）, positioned at the enterprising material mouth of quiet disk
The both sides of lower end, shower nozzle and liquid nitrogen container（9）It is connected by pipeline, there is 1 piston on each shower nozzle（14）With 2 check valves
（15、16）, can be by independent cooling motor（12）With cooling drive system（13）Driving eject liquid nitrogen to abrasive material storehouse.
There is temperature sensing meter near material contact surface on quiet disk（11）, it is logical that temperature sensing meter and pin grind outside computer
Cross data wire to carry out data transmission, so as to realize monitoring, warning, record to abrasive material storehouse temperature.Drive the cooling motor of shower nozzle
（12）Also it is connected with computer, the ejection frequency of computer software control shower nozzle, the monitoring number of combination temperature surveymeter can be passed through
According to the achievable intelligent control to abrasive material storehouse temperature.
Embodiment 2, a kind of low temperature pin for preparing cell culture medium with cooling sandwith layer grind
A kind of low temperature pin for preparing cell culture medium grinds, as shown in Fig. 2 by housing（1）, charging aperture（2）, quiet disk
（3）, Moving plate（4）, discharging opening（5）, mair motor（6）And drive system（7）Composition, wherein quiet disk is located at the top of Moving plate, quiet disk
Space between Moving plate constitutes abrasive material storehouse, has 10 coolant shower nozzles in the central authorities in abrasive material storehouse（8）, positioned at the enterprising material mouth of quiet disk
Around lower end, annularly（Radiation symmetric）Equably arrange, shower nozzle and liquid nitrogen container（9）It is connected by pipeline, on each shower nozzle
There is 1 piston（14）With 2 check valves（15、16）, can be by independent cooling motor（12）With cooling drive system（13）'s
Drive to abrasive material storehouse and eject liquid nitrogen.There is cooling sandwith layer between the outer wall and inwall of housing（10）, filled with liquid nitrogen in interlayer.
There is temperature sensing meter near material contact surface on quiet disk（11）, it is logical that temperature sensing meter and pin grind outside computer
Cross radio wave to carry out data transmission, so as to realize monitoring, warning, record to abrasive material storehouse temperature.Drive the cooling electricity of shower nozzle
Machine（12）Also it is connected with computer, the ejection frequency of computer software control shower nozzle, the monitoring of combination temperature surveymeter can be passed through
Data, are capable of achieving the intelligent control to abrasive material storehouse temperature.
The using method of embodiment 3, low temperature pin mill
Connect mair motor（6）With cooling motor（12）Power supply, the Moving plate under driven by power（4）Relative to quiet disk（3）Rotate,
And outside centrifugal force is produced, by cell culture based raw material to be comminuted from charging aperture input needle mill, in centrifugal action
Lower material is moved to outer rim, the quiet pin between material particles and on material particles and quiet disk（17）, dynamic pin on Moving plate（18）
Collide, crush, the material of crushing is from discharging opening（5）Out.While coolant shower nozzle（8）In cooling motor（12）And cooling
Drive system（13）In the presence of draw from liquid nitrogen container and liquid nitrogen and be ejected in abrasive material storehouse, liquid nitrogen gasifies rapidly after spraying,
Formation is significantly lowered the temperature, and cold air is moved rapidly to abrasive material storehouse outer rim in the case where centrifugal force drives, diffusion, full of abrasive material storehouse, to crushing
In material particles and dynamic pin, quiet pin directly lowered the temperature.Arranged by computer software, when detected by temperature sensing meter
When temperature is less than -20 DEG C, cooling motor is closed, stop cooling；When the temperature detected by temperature sensing meter is higher than 20 DEG C, plus
The rotating speed of fast cooling motor, accelerates the liquid nitrogen spray frequency, increase cooling intensity.
Claims (7)
1. it is a kind of for prepare cell culture medium low temperature pin grind, by housing, charging aperture, quiet disk, Moving plate, discharging opening, motor and
Drive system is constituted, wherein the space between quiet disk and Moving plate constitutes abrasive material storehouse, it is characterised in that have one in the central authorities in abrasive material storehouse
Individual or multiple coolant shower nozzles, shower nozzle is connected with cooling liquid container by pipeline, and shower nozzle can spray under pressure to abrasive material storehouse
Project coolant.
2. the low temperature pin described in claim 1 grinds, it is characterised in that described quiet disk is located at the top of Moving plate, described cooling
Liquid shower nozzle is located on quiet disk.
3. the low temperature pin described in claim 2 grinds, it is characterised in that the quantity of described coolant shower nozzle is 2 to 10, distribution
It is in the both sides of charging aperture lower end or annular in shape evenly distributed around charging aperture lower end.
4. the low temperature pin described in claim 1 grinds, it is characterised in that have cooling sandwith layer between the outer wall and inwall of housing, presss from both sides
Filled with coolant in layer.
5. the low temperature pin described in claim 1 grinds, it is characterised in that have temperature sensing meter in Jing Panshang, temperature sensing meter is obtained
The data for obtaining can be transferred on the computer outside pin mill or LCDs, alarm, monitor.
6. the low temperature pin described in claim 1 grinds, it is characterised in that described coolant shower nozzle is by independent motor and power train
A completely drive control, the motor for driving shower nozzle is connected with computer, can be by the ejection frequency of computer software control shower nozzle.
7. the low temperature pin described in claim 1 grinds, and described coolant is liquid nitrogen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621039281.2U CN206082684U (en) | 2016-09-06 | 2016-09-06 | A low temperature needle grinds for preparing cell culture base |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621039281.2U CN206082684U (en) | 2016-09-06 | 2016-09-06 | A low temperature needle grinds for preparing cell culture base |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206082684U true CN206082684U (en) | 2017-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621039281.2U Active CN206082684U (en) | 2016-09-06 | 2016-09-06 | A low temperature needle grinds for preparing cell culture base |
Country Status (1)
| Country | Link |
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| CN (1) | CN206082684U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112474019A (en) * | 2020-11-12 | 2021-03-12 | 成都武侯甘露海藏医院有限公司 | Powdering method of Tibetan medicine raw material |
-
2016
- 2016-09-06 CN CN201621039281.2U patent/CN206082684U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112474019A (en) * | 2020-11-12 | 2021-03-12 | 成都武侯甘露海藏医院有限公司 | Powdering method of Tibetan medicine raw material |
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