CN206014950U - A kind of multi-functional Tissue Culture Dish of buffer-type - Google Patents
A kind of multi-functional Tissue Culture Dish of buffer-type Download PDFInfo
- Publication number
- CN206014950U CN206014950U CN201621048825.1U CN201621048825U CN206014950U CN 206014950 U CN206014950 U CN 206014950U CN 201621048825 U CN201621048825 U CN 201621048825U CN 206014950 U CN206014950 U CN 206014950U
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- China
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- relief area
- wall
- culture dish
- inwall
- relief
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Abstract
This utility model provides a kind of multi-functional Tissue Culture Dish of buffer-type, including operating space, relief area and cultivation region；The culture dish is from outer to inner sequentially provided with culture dish side wall, relief area outer wall, relief area inwall；It is operating space between culture dish side wall and relief area outer wall, is relief area between the relief area outer wall and relief area inwall, is cultivation region in the relief area inwall；The relief area outer wall and relief area inner wall lower difference relief area unit with grooves in outer wall and relief area inboard wall groove, are fissure of displacement structure between relief area unit with grooves in outer wall and relief area inboard wall groove.The Tissue Culture Dish simple structure, workable, multiple functional, Deca can not only be reduced and extract impact of the liquid to cell culture area, and be also convenient for cell growth state and observed and contrasted, the operation such as the cut being conveniently used in " wound healing " experiment for detect cell migration motor capacity and the positioning of shot region.
Description
Technical field
This utility model is related to a kind of Tissue Culture Dish, more particularly to a kind of multi-functional Tissue Culture Dish of buffer-type, belongs to
Biomedical sector.
Background technology
As China's economy is developed rapidly with social, medical treatment and biomedical sector are increasingly paid attention to by country.
Research in terms of cytobiology is always the most basic research method of biomedical research, and cell culture system is whole biological
There is in medical research important effect.During cell culture, often adherent cell is caused because changing the operation such as liquid
In impact, so as to affect the normal growth of attached cell；The culture dish for using at present can not carry out what cell growth quantitatively changed
Observation, construction are relatively simple, and function is more single, it is difficult to meet the demand of existing research.
Utility model content
Technical problem：For the deficiency for solving the above problems, this utility model provides a kind of multi-functional cell of buffer-type
Culture dish.
Technical scheme：The multi-functional Tissue Culture Dish of a kind of buffer-type that this utility model is provided, including operating space, relief area
And cultivation region；The culture dish is from outer to inner sequentially provided with culture dish side wall (1), relief area outer wall (2), relief area inwall (3)；
It is operating space, relief area outer wall (2) and relief area inwall (3) between culture dish side wall (1) and relief area outer wall (2)
Between be relief area, be cultivation region in relief area inwall (3)；Relief area outer wall (2) and relief area inwall (3) bottom
Difference relief area unit with grooves in outer wall (7) and relief area inboard wall groove (8), relief area unit with grooves in outer wall (7) and relief area inboard wall groove (8)
Between be fissure of displacement structure, relief area unit with grooves in outer wall (7) and relief area inboard wall groove (8) have.
Used as improvement, base plate (6) bottom of cultivation region is provided with grid (4), and the point of intersection of grid (4) is respectively equipped with together
Heart annulus (5)；Grid (4) live width meets the needs of basis of microscopic observation, and each intersection point has and can recognize under the microscope
Particular number, donut diameter are determined according to microscopical observation multiple and scope.The labelling system of culture dish bottom is convenient
Observation and contrast to specific region cellular localization and cell growth situation.
Beneficial effect：The multi-functional Tissue Culture Dish simple structure of buffer-type that this utility model is provided, workable, work(
Can be complete, Deca can not only be reduced and extract impact of the liquid to cell culture area, and be also convenient for cell growth state entering
Row observation and contrast, the cut being conveniently used in " wound healing " experiment for detect cell migration motor capacity and shot region
The operation such as positioning.
The present invention is divided into operating space and cultivation region by relief area Tissue Culture Dish, and relief area is by with groove structure
Relief area outer wall (2), relief area inwall (3) composition, the groove on relief area outer wall (2) and relief area inwall (3) form the fissure of displacement
Structure, the fissure of displacement structure have three functions：
(1) Nostoc commune Vanch ware can produce ripple and the arrival that water ripples effect, i.e. Deca liquid are produced in Deca liquid
The ripple that culture dish inwall is reflected produces reinfocing effect, larger to cell culture environment disturbance, and culture dish of the present invention is in behaviour
When making area's Deca liquid, due to the presence of fissure of displacement groove relief area, most of water ripples of formation only can be propagated in operating space, warp
Fissure of displacement groove is propagated to the water ripples of cultivation region and can be offset by the arrangement of groove structure that (propagation path difference isλ is wavelength), so as to reduce the interference to cultivation region cell；
(2) according to hydrodynamics, flow velocity is inversely proportional to path is flowed through, when moving and adding culture fluid in operating space, culture
The culture fluid in area need to flow through relief area, due to the presence of relief area inside and outside wall upper groove, increase the path that flows through of culture fluid, stream
Speed reduces, and moves impact of the liquid feeding body to cultivation region cell so as to reduce；
(3), when making liquid generation head difference in culture dish due to mobile culture dish, relief area increases and flows through path, reduces
Flow velocity, so that reduce the interference to cultivation region cell growth environment.
Description of the drawings
Fig. 1 is the structural representation of the multi-functional Tissue Culture Dish of this utility model buffer-type.
Fig. 2 is the top view of the multi-functional Tissue Culture Dish of this utility model buffer-type.
Specific embodiment
Below in conjunction with accompanying drawing, the utility model is described in more detail.
The multi-functional Tissue Culture Dish of buffer-type, including operating space, relief area and cultivation region；The culture dish from outer to inner according to
Secondary it is provided with culture dish side wall (1), relief area outer wall (2), relief area inwall (3)；Culture dish side wall (1) and relief area outer wall
(2) it is operating space between, is relief area between relief area outer wall (2) and relief area inwall (3), the relief area inwall
(3) it is cultivation region in；Relief area outer wall (2) and relief area inwall (3) bottom difference relief area unit with grooves in outer wall (7) and buffering
Area's inboard wall groove (8), is fissure of displacement structure between relief area unit with grooves in outer wall (7) and relief area inboard wall groove (8), and relief area outer wall is recessed
Groove (7) and relief area inboard wall groove (8) have specific arrangement and shape.Used as improvement, base plate (6) bottom of cultivation region is provided with
Grid (4), the point of intersection of grid (4) are respectively equipped with donut (5)；Grid (4) live width meets basis of microscopic observation
Needs, each intersection point has a particular number that can be recognized under the microscope, and donut diameter is according to microscopical observation times
Number and scope determine.
The culture dish using method：
(1) carry out moving liquid feeding gymnastics work in operating space；
(2), during observation of cell adherent growth state, the numbering of circle and cell quantity under microscope multiple are recorded, when specific
Between be spaced after continue observation and record circle numbering and cell quantity, by contrast obtain cell growth situation；
(3) along grid, horizontally or vertically adherent iuntercellular is marked " scar " of specific width in line direction, is passed through
" healing " situation of observation " scar " place cell is judging migration and the motion conditions of cell.
Claims (2)
1. the multi-functional Tissue Culture Dish of a kind of buffer-type, it is characterised in that：Including operating space, relief area and cultivation region；The training
Foster ware is from outer to inner sequentially provided with culture dish side wall (1), relief area outer wall (2), relief area inwall (3)；Culture dish side wall
(1) it is operating space and relief area outer wall (2) between, is relief area between relief area outer wall (2) and relief area inwall (3),
It is cultivation region in relief area inwall (3)；Outside the difference relief area of relief area outer wall (2) and relief area inwall (3) bottom
Wall groove (7) and relief area inboard wall groove (8), tie for the fissure of displacement between relief area unit with grooves in outer wall (7) and relief area inboard wall groove (8)
Structure.
2. the multi-functional Tissue Culture Dish of a kind of buffer-type according to claim 1, it is characterised in that：The base plate of cultivation region
(6) bottom is provided with grid (4), and the point of intersection of grid (4) is respectively equipped with donut (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621048825.1U CN206014950U (en) | 2016-09-12 | 2016-09-12 | A kind of multi-functional Tissue Culture Dish of buffer-type |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621048825.1U CN206014950U (en) | 2016-09-12 | 2016-09-12 | A kind of multi-functional Tissue Culture Dish of buffer-type |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206014950U true CN206014950U (en) | 2017-03-15 |
Family
ID=58259241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621048825.1U Expired - Fee Related CN206014950U (en) | 2016-09-12 | 2016-09-12 | A kind of multi-functional Tissue Culture Dish of buffer-type |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206014950U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113784618A (en) * | 2019-04-18 | 2021-12-10 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
-
2016
- 2016-09-12 CN CN201621048825.1U patent/CN206014950U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113784618A (en) * | 2019-04-18 | 2021-12-10 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
| CN113784618B (en) * | 2019-04-18 | 2023-12-22 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
| US11889830B2 (en) | 2019-04-18 | 2024-02-06 | Abs Global, Inc. | System and process for continuous addition of cryoprotectant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170315 Termination date: 20180912 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |