CN205990388U - Immobilized cell carbon fiber ball reactor for fermentation and its brewing fermentation system - Google Patents

Immobilized cell carbon fiber ball reactor for fermentation and its brewing fermentation system Download PDF

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Publication number
CN205990388U
CN205990388U CN201620335776.3U CN201620335776U CN205990388U CN 205990388 U CN205990388 U CN 205990388U CN 201620335776 U CN201620335776 U CN 201620335776U CN 205990388 U CN205990388 U CN 205990388U
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China
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fermentation
reactor
carbon fiber
immobilized cell
fiber ball
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CN201620335776.3U
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Chinese (zh)
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薛正楷
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Luzhou Vocational and Technical College
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Luzhou Vocational and Technical College
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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The utility model discloses a kind of immobilized cell carbon fiber ball reactor for fermentation, including the fermentation reactor body for containing immobilized cell carbon fiber ball, described fermentation reactor body surface is evenly equipped with through hole;Described fermentation reactor is body martensitic stain less steel hollow ball, and described rustless steel hollow ball includes being constituted, with detachable being fixedly connected, episphere and the lower semisphere that can accommodate immobilized cell carbon fiber ball;Using the controlled balling-up of carbon fiber, anti- soda acid, corrosion resistant feature, through the anti-soda acid with magnetic, corrosion resistant multiporous biological reactor combines, in sweat, magnetic container can meet abundant and fermentation raw material time of contact and contact area, there is high mass transfer efficiency, either the time is still spatially, vital movement on material all assumes three-dimensional multiformity, only need to be hung by electric magnetic after fermentation ends and draw out stainless steel ball from pit, lower round fermentation can be carried out after of short duration activation again, the fermentation of many rounds can be carried out, simplify operating procedure, save labour force, improve production efficiency simultaneously.

Description

Immobilized cell carbon fiber ball reactor for fermentation and its brewing fermentation system
Technical field
This utility model is related to brewing technical field, particularly to anti-for the immobilized cell carbon fiber ball for fermentation Answer device brewing fermentation system.
Background technology
Immobilized cell is the new technique growing up on the basis of immobilized enzyme, that is, will using chemically or physically means Free cell be positioned limit area of space and make its keep activity and can be with a kind of basic technology of Reusability.Immobilization Cell maintains the vital movement ability of cell, and the fermentation of its not only specific ionization cell has more superiority, and compares immobilization Enzyme has more advantages, such as:It eliminates smudge cellses to extract the formality of endocellular enzyme, reduces preparation cost;Cell growth Dead time is short, and cell is many, and reaction is fast, and contamination resistance is strong, can be with continuous fermentation, Reusability, and application cost is low;Enzyme is thin In the environment of intracellular, stability is higher, after intact cell immobilization, the loss of enzyme activity is less etc..Although immobilized cell technology Have broad application prospects, but be to be studied on a laboratory scale mostly, really practical or commercial Application, also Problems with needs to solve:1) stability problem of immobilized cell, because immobilized material is to the toxicity of microorganism, fixation Heat release in change condition, immobilization process etc. all has an impact to cell and makes part cell inactivation;2) immobilized cell batch is raw Produce the exploitation of device, this is that immobilized cell technology moves towards the essential step of practical application from laboratory;3) the consolidating of cheap and durable Surely the research and development of carrier and compound immobilization technology are changed;4) exploitation of efficient immobilization reactor.Currently, immobilized cell The production capacity of technology and the feasibility industrially applied, depend greatly on the construction of reactor.Therefore, these The successful solution of problem, particularly immobilized cell produce in batches device, that is, the exploitation of immobilized cell bioreactor for Immobilized cell technology extensive application industrially will play the effect of being greatly promoted, and the production significantly improving factory is imitated Rate and saving production cost, thus effectively improve the competitiveness of China's product.In existing immobilized cell fermentation, typically adopt With single bacterial strain bacterial strain purebred or limit bacterial strain mixed fermentation, the bioreactor that it adopts is baroque airtight appearance Device solid-state fermentation reactor, it has the drawback that in sweat, mass-transfer efficiency is low, needs by manually right after fermentation ends Reactor is carried, and equipment investment is big, and operation energy consumption is high, continuous prodution difficult to realize, in particular for the fermentation of many rounds, Its manipulation strength and difficulty are all larger, are difficult to meet the demand of many round fermentations.
Utility model content
In view of this, the purpose of this utility model be to provide a kind of for the immobilized cell carbon fiber ball for fermentation Reactor, enables magnetic container in sweat to meet abundant and fermentation raw material time of contact and contact area, has high mass transfer Efficiency, can carry out the fermentation of many rounds, only need to be hung by electric magnetic and draw out stainless steel ball from pit after fermentation ends, pass through Lower round fermentation can be carried out after of short duration activation again, simplify operating procedure, save labour force, improve production efficiency simultaneously.
Immobilized cell carbon fiber ball reactor for fermentation of the present utility model, including for containing immobilized cell The fermentation reactor body of carbon fiber ball, described fermentation reactor body surface is evenly equipped with through hole;
Further, described fermentation reactor body martensitic stain less steel hollow ball, described rustless steel hollow ball includes with can Dismountable is fixedly connected and constitutes episphere and the lower semisphere that can accommodate immobilized cell carbon fiber ball;
Further, described episphere and lower semisphere are the connection that is screwed, described episphere/lower semisphere opening edge appearance Face is provided with external screw thread, and described lower semisphere/episphere opening edge inner surface setting has female thread;
Further, in the hollow ball surface of rustless steel, it is empty that described through hole total surface area accounts for rustless steel to described through hole radial distribution The 10-20% on bulbus cordis surface;
Further, a diameter of 10-15cm of described rustless steel hollow ball, the aperture of described through hole is 0.2-0.4cm.
This utility model is also disclosed a kind of brewing fermentation system, including round and the splendid attire being positioned in round There is the magnetic fermentation reactor of immobilized cell carbon fiber ball and hung by the electric magnetic that magnetic force picks and places fermentation reactor, send out Ferment carries out lower whorl time fermentation after terminating after electric magnetic draws out the activated process of fermentation reactor from round;
Further, described round is pit, and described fermentation reactor is with 8-12/m2Put into pit upper strata, middle level Or lower floor;
Further, the immobilized cell carbon fiber ball loadings of described fermentation reactor are less than fermentation reactor volume 80-90%;
Further, a diameter of 0.6-2cm of described immobilized cell carbon fiber ball.
The beneficial effects of the utility model:Immobilized cell carbon fiber ball fermentation reaction for fermentation of the present utility model Device and its brewing fermentation system, using the controlled balling-up of carbon fiber, anti-soda acid, corrosion resistant feature, through with the anti-soda acid of magnetic, resistance to The multiporous biological reactor of corrosion combines, in sweat magnetic container can meet abundant with fermentation raw material time of contact with connect Contacting surface is amassed, and has high mass transfer efficiency, and either still spatially the vital movement on material all assumes three-dimensional multiformity the time, Only need to be hung by electric magnetic after fermentation ends and draw out stainless steel ball from round, can enter after of short duration activation again The lower round fermentation of row, can carry out the fermentation of many rounds, simplify operating procedure, save labour force, improve production efficiency simultaneously.
Brief description
With reference to the accompanying drawings and examples this utility model is further described:
Fig. 1 is the immobilized cell carbon fiber ball fermentation reactor structural representation for fermentation of the present utility model.
Fig. 2 is the cross-sectional view of the fermentation reactor equipped with immobilized cell carbon fiber ball;
Fig. 3 is the overlooking the structure diagram of brewing fermentation system.
Specific embodiment
Immobilized cell carbon fiber ball fermentation reactor for fermentation of the present utility model, including for containing immobilization The fermentation reactor body 1 being provided with lid of cell carbon fibrous nodules 3, described fermentation reactor body 1 surface is evenly equipped with through hole 2;There is anti-soda acid, carry out in the corrosion-resistant and porous magnetic container of magnetic by filling in immobilized cell carbon fiber ball 3 Semicontinuous fermentation, good mixing effect, meet sufficient time of contact and contact area in sweat, there is high mass transfer effect Rate, can carry out the fermentation of many rounds, and the uniform through hole 2 on surface is to produce bacterium cell to absorb in environment nutrient substance and carry out mass transfer Passage is it is ensured that the macroscopical mass transport process in solid ferment process is high with microcosmic mass-transfer efficiency, according to the cavity volume of round body 1 Size loads several carbon fiber balls, and the gap between carbon fiber ball forms mass transfer channel, makes mass transfer evenly, and fermentation is more filled Point, after fermentation ends, only by electric magnetic equipment (as electric magnetic hangs), fermentation reactor body 1 need to be drawn out, Without a dead lift, efficiency high, save labour force, and do not affect the environment of pit 4, fermentation reactor body 1 warp that will take out Lower round fermentation directly can be entered after activated process again.
In the present embodiment, described fermentation reactor body 1 martensitic stain less steel hollow ball, described rustless steel hollow ball includes Episphere and the lower semisphere that immobilized cell carbon fiber ball 3 can be accommodated is constituted with detachable being fixedly connected;Two halves spheroid is Lid is also reservoir body, detachable be fixedly connected constitute accommodate immobilized cell carbon fiber ball 3 cavity facilitate feed supplement it is ensured that The carrying out of semicontinuous fermentation, the diameter of carbon fiber ball directly affects the diffusion flux of material, the most efficient in impact sweat Mass-transfer efficiency, therefore, the diameter of carbon fiber ball is excessive or the too small diffusion being all unfavorable for material, and then affects mass-transfer efficiency, Only the size of the size of rustless steel hollow ball 1 and carbon fiber ball is matched and spread velocity of liquid assets between guarantee material, enter And reach highest mass-transfer efficiency, and therefore, a diameter of 0.6-2cm of described carbon fiber ball, preferably 1cm.
In the present embodiment, described episphere and lower semisphere are the connection that is screwed, described episphere/lower semisphere opening edge Outer surface is provided with external screw thread, and described lower semisphere/episphere opening edge inner surface setting has female thread;Structure is simple, Easily operated, repeat and utilize, utilization rate of equipment and installations is high.
In the present embodiment, described through hole 2 radial distribution accounts for not in the hollow ball surface of rustless steel, described through hole 2 total surface area The 10-20% of the rust hollow ball surface of steel;It is preferably described through hole 2 total surface area and account for the 15% of the hollow ball surface of rustless steel;Uniformly Through hole 2 be that fermented cells absorb nutrient substance in environment and the passage that carries out mass transfer it is ensured that macroscopic view in solid ferment process Mass transport process is high with microcosmic mass-transfer efficiency, and the therefore distribution of through hole 2 is unsuitable excessive, excessively can affect the bulk strength of structure, Unsuitable very few, the smoothness of impact mass transfer channel that I haven't seen you for ages excessively, and then affect mass transfer velocity.
In the present embodiment, a diameter of 10-15cm of described rustless steel hollow ball, the aperture of described through hole 2 is 0.2-0.4cm; Being preferably aperture is 0.3cm;The size of the size of rustless steel hollow ball and carbon fiber ball is matched and expands between guarantee material Scattered velocity of liquid assets, and then reach highest mass-transfer efficiency.
The brewing fermentation system of the present embodiment, including round 4 be positioned over and be loaded with immobilization in round 4 The magnetic fermentation reactor 1 of cell carbon fibrous nodules 3 and being hung by the electric magnetic that magnetic force picks and places fermentation reactor 1, fermentation knot Carry out lower whorl time fermentation after electric magnetic draws out the activated process of fermentation reactor 1 from round 4 after bundle;Sending out After ferment terminates, only by electric magnetic equipment (as electric magnetic hangs), fermentation reactor 1 need to be drawn out, need not manually remove Fortune, efficiency high, save labour force, and do not affect the environment of pit 4, by the fermentation reactor 1 taking out through directly activated process Lower round fermentation can be entered afterwards again.
In the present embodiment, described round 4 is pit, and described fermentation reactor 1 is with 8-12/m2Put into pit 4 Upper strata, middle level or lower floor;It is preferably 10/m2;Form effective mass transfer channel between fermentation reactor 1 in round 4, Ensure the discharge automatically adjusting with fermentation gas product of organism in fermentation reactor 1, make the macroscopic view in solid ferment process Mass transport process is high with microcosmic mass-transfer efficiency.
In the present embodiment, immobilized cell carbon fiber ball 3 loadings of described fermentation reactor 1 are less than fermentation reactor The 80-90% of 1 volume;It is preferably 85%;Several immobilized cell carbon fibers are loaded according to the cavity volume size of fermentation reactor 1 Ball 3, the gap between immobilized cell carbon fiber ball 3 forms mass transfer channel, makes mass transfer evenly, and fermentation is more abundant.
In the present embodiment, a diameter of 0.6-2cm of described immobilized cell carbon fiber ball 3;It is preferably 1.5cm;It is provided with The mass transfer channel of effect, the diameter of immobilized cell carbon fiber ball 3 directly affects the diffusion flux of material, in impact sweat Efficient mass-transfer efficiency, therefore, the diameter of carbon fiber ball is excessive or the too small diffusion being all unfavorable for material, and then affects mass transfer effect The size of the size of fermentation reactor 1 and carbon fiber ball 3 is only matched and spreads velocity of liquid assets between guarantee material by rate, And then reach highest mass-transfer efficiency.
Finally illustrate, above example only in order to the technical solution of the utility model to be described and unrestricted, although ginseng According to preferred embodiment, this utility model is described in detail, it will be understood by those within the art that, can be to this The technical scheme of utility model is modified or equivalent, the objective without deviating from technical solutions of the utility model and model Enclose, it all should be covered in the middle of right of the present utility model.

Claims (9)

1. a kind of for fermentation immobilized cell carbon fiber ball reactor it is characterised in that:Including for containing fixation cell The magnetic fermentation reactor body of born of the same parents' carbon fiber ball, described fermentation reactor body surface is evenly equipped with through hole.
2. according to claim 1 for fermentation immobilized cell carbon fiber ball reactor it is characterised in that:Described Ferment reactor body is martensitic stain less steel hollow ball, and described rustless steel hollow ball includes can with the detachable composition that is fixedly connected Accommodate episphere and the lower semisphere of immobilized cell carbon fiber ball.
3. according to claim 2 for fermentation immobilized cell carbon fiber ball reactor it is characterised in that:On described Hemisphere and lower semisphere are the connection that is screwed, and described episphere/lower semisphere opening edge outer surface is provided with external screw thread, described institute Stating lower semisphere/episphere opening edge inner surface setting has female thread.
4. according to right 1 for fermentation immobilized cell carbon fiber ball reactor it is characterised in that:Described through hole footpath To being distributed in fermentation reactor body surface, described through hole total surface area accounts for the 10-20% that fermentation reactor body surface amasss.
5. according to claim 3 for fermentation immobilized cell carbon fiber ball reactor it is characterised in that:Described not The rust a diameter of 10-15cm of steel hollow ball, the aperture of described through hole is 0.2-0.4cm.
6. a kind of brewing fermentation system it is characterised in that:Including round be positioned over and be loaded with fixation in round Change the magnetic fermentation reactor of cell carbon fibrous nodules and hung by the electric magnetic that magnetic force picks and places fermentation reactor, fermentation ends Carry out lower whorl time fermentation after electric magnetic draws out the activated process of fermentation reactor from round afterwards.
7. according to claim 6 wine brewing reactor it is characterised in that:Described round is pit, and described fermentation is anti- Answer device with 8-12/m2Put into pit upper strata, middle level or lower floor.
8. brewing fermentation reactor according to claim 7 it is characterised in that:Fixation cell in described fermentation reactor Born of the same parents' carbon fiber ball loadings are less than the 80-90% of fermentation reactor volume.
9. brewing fermentation reactor according to claim 7 it is characterised in that:Described immobilized cell carbon fiber ball straight Footpath is 0.6-2cm.
CN201620335776.3U 2016-04-19 2016-04-19 Immobilized cell carbon fiber ball reactor for fermentation and its brewing fermentation system Withdrawn - After Issue CN205990388U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106010851A (en) * 2016-04-19 2016-10-12 泸州职业技术学院 Built-in immobilized cell carbon fiber ball reactor for fermentation container and wine brewing system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106010851A (en) * 2016-04-19 2016-10-12 泸州职业技术学院 Built-in immobilized cell carbon fiber ball reactor for fermentation container and wine brewing system
CN106010851B (en) * 2016-04-19 2019-03-26 泸州职业技术学院 Built-in immobilized cell carbon fiber ball reactor and its brewing system for round

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