CN205982290U - A reagent bottle for chemiluminescent immunoassay detects - Google Patents
A reagent bottle for chemiluminescent immunoassay detects Download PDFInfo
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- CN205982290U CN205982290U CN201621013681.6U CN201621013681U CN205982290U CN 205982290 U CN205982290 U CN 205982290U CN 201621013681 U CN201621013681 U CN 201621013681U CN 205982290 U CN205982290 U CN 205982290U
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Abstract
The utility model discloses a reagent bottle for chemiluminescent immunoassay detects, this reagent bottle hold the reagent that has two kind at least different grade types and include bottle and lid, and inside the partition by the separator of bottle is two mutually independent upward cavity and lower cavity, and it has different reagent indoor holding respectively to go up cavity and cavity of resorption be equipped with one -way passage on the separator, the indoor reagent of cavity of resorption can be through cavity in the one -way passage inflow, cavity under the indoor reagent of epicoele can not flow into through one -way passage. The utility model discloses a two kinds of reagent can effectively be separated to the reagent bottle, and the accessible uses preceding turn upside down reagent bottle with two kinds of abundant mixings of reagent to avoid the long -time mixed solid that causes of two kinds of reagent to appear just, makes reagent reach best effect before the use, has promoted the performance of reagent.
Description
Technical field
This utility model be related to in-vitro diagnosis field of medical examination and in particular to a kind of for chemiluminescence immunoassay detection
Reagent bottle.
Background technology
Last century the mid-1970s Arakawe reported first carries out enzyme immune detection with luminous signal, using luminous change
Learn reaction detection ultra micro quantity of material, check ultramicron active substance especially in clinical immunization detection.Chemiluminescence immunoassay
Detection (Chemiluminescence Immunoassay, CLIA) is by chemiluminescence or bioluminescence system and immunoreation
Combine, for detecting a kind of novel markings immunoassay, its Cleaning Principle and the radioimmunity of trace antigen or antibody
(RIA) and enzyme immunity (EIA) is similar, it is a difference in that and radionuclide or enzyme are replaced as label using luminescent substance, and borrow
The luminous intensity helping its own is directly measured.
Chemiluminescence immunoassay detects, has both had the high sensitivity of radioimmunity, and detection range is wide, has enzyme linked immunological again
It is easy to not use harmful reagent in normalizing operation, and test, reagent keeps the phase long to easy and simple to handle, quick feature, application
In biology, medical research and clinical experiment diagnostic work, become in on-radiation immunodetection the most promising method it
One.
As a kind of on-radiation immunolabelling technique, except having, sensitivity is high, specificity is good, mark for electrochemiluminescent immunoassay detection
Outside the features such as note thing good stability, its detection program is also easy, quick, only needs micro specimen, can be examined at short notice
Survey result.Clinically it has been applied to the correlations such as various lemology, tumor markerses, hormoness, cardiovascular and cerebrovascular disease in recent years
The diagnosis of project, respond well in terms of Clinical detection, it is to replace Enzyme-multiplied immune technique (ELISA) in coming few decades to become
One of detection method of post.
In actual use, may there is the situation of plurality of reagents, but the long-term mixing between plurality of reagents may go out
Now separate out precipitation equistability problem, therefore multiple reagent in test kit, occur；And because chemical luminescence reagent kit is using entirely certainly
Dynamic instrumentation test, operating procedure is limited to be loaded it is impossible to draw plurality of reagents simultaneously, therefore the number of seminal plasma fructose detection kit
There is limitation.Tested it is necessary to now mix reagent when reagent number is unable to reach the reagent test number of instrument permission.
However, now mix reagent to be readily incorporated pollutant, thus the problems such as cause test result inaccurate.
Utility model content
The purpose of this utility model is to provide a kind of reagent bottle for chemiluminescence immunoassay detection, and described reagent bottle accommodates
There are at least two different types of reagent, described reagent bottle includes bottle and lid, is isolated part and is divided into inside described bottle
Two separate upper chamber and lower chambers, described upper chamber and bottom chamber accommodate different reagent, respectively described
Separator is provided with half-duplex channel, and the reagent of described bottom chamber can flow into upper chamber by described half-duplex channel, and described
The indoor reagent of epicoele can not flow into described lower chambers by described half-duplex channel, needing the indoor reagent of described epicoele and
When the reagent of described bottom chamber is mixed, described reagent bottle is inverted, the reagent of described bottom chamber is by described unidirectional
Passage flows into described epicoele interior, thus realize the indoor reagent of described epicoele mixing with the reagent of described bottom chamber.
In one embodiment, described half-duplex channel is the through hole being arranged on described separator, and sets in described through hole
There is check valve, the entrance of described check valve is towards described lower chambers, and the outlet of described check valve is towards described upper chamber.
It is preferred that described through hole is arranged at the middle part of described separator, and separator described in the Kong Xinyu of described through hole
Center substantially overlaps.
It is preferred that the volume of described upper chamber is more than or equal to the reagent of described bottom chamber and the examination of described epicoele interior
The capacity sum of agent.
It is preferred that the volume V of described upper chamber1Volume V with described lower chambers2Meet following relation：V1/V2=3/1.
In one embodiment, described half-duplex channel is the through hole being arranged on described separator, and the diameter of described through hole is from upper
Down reduce, thus described through hole is the through hole with taper, be provided with described through hole and cone seal plug, described taper is close
Blocking and described through hole cooperation, thus when described reagent bottle is just put, described through hole is sealed by described cone seal plug, and in institute
When stating reagent bottle inversion, the reagent of described bottom chamber can be by the slit flow between described cone seal plug and described through hole
Enter described epicoele interior, thus realize the indoor reagent of described epicoele mixing with the reagent of described bottom chamber.
It is preferred that described through hole is arranged at the middle part of described separator, and separator described in the Kong Xinyu of described through hole
Center substantially overlaps, and the volume of described upper chamber is more than or equal to the reagent of described bottom chamber and described epicoele interior
The capacity sum of reagent.
In one embodiment, form a circle groove in the upper surface of described separator around all portions of this separator, described
It is provided with multiple through holes in groove, in described groove, is provided with sealing ring, when described reagent bottle is just put, described sealing ring will be described
Through hole sealing in groove, and when described reagent bottle is to inversion, the reagent of described bottom chamber can be by described groove
Through hole flow into described upper chamber, thus realize the indoor reagent of described epicoele mixing with the reagent of described bottom chamber.
In one embodiment, the lower chambers of described reagent bottle are equipped with multiple nonionic surfactants, and described reagent bottle
Upper chamber equipped with free thyroxine enzyme diluent and enzyme conjugates.
It is preferred that described separator is integrally formed with described bottle.
Reagent bottle of the present utility model can separate two kinds of solution effectively, it is to avoid what the long-time mixing of two kinds of solution caused consolidates
Body separates out and can fully mix two kinds of solution by using front upper and lower reverse reagent, makes reagent reach best effect before use
Really, improve the performance of reagent.
Brief description
Fig. 1 is the structural representation of the reagent bottle of this utility model first embodiment；
Fig. 2 is the structural representation of the reagent bottle of the another second embodiment of this utility model；And
Fig. 3 is the structural representation of the reagent bottle of this utility model 3rd embodiment.
Specific embodiment
Below with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail, to become apparent from understanding this reality
With new objects, features and advantages.It should be understood that embodiment shown in the drawings is not the limit to this utility model scope
System, and simply to illustrate that the connotation of technical solutions of the utility model.
The reagent bottle of this utility model first embodiment is described with reference to Fig. 1.
Fig. 1 is the structural representation of the reagent bottle 100 of this utility model one embodiment, as shown in figure 1, the use of the present embodiment
Reagent bottle 100 (hereinafter referred to as reagent bottle 100) in chemiluminescence immunoassay detection includes bottle 1 and lid 2, bottle 1 interior
Portion is isolated part 3 and is divided into two separate upper chamber 10 and lower chambers 20, holds respectively in upper chamber 10 and lower chambers 20
Receiving has different reagent, and such as lower chambers 20 are built with multiple nonionic surfactants, and upper chamber 10 is built with free first
Shape parathyrine enzyme diluent and enzyme conjugates etc..
Separator 3 is provided with half-duplex channel 31, and the reagent in lower chambers 20 can flow into upper chamber 10 by half-duplex channel 31
Interior, and the reagent in upper chamber 10 can not flow in lower chambers 20 by half-duplex channel 31, needing the examination in upper chamber 20
When agent is mixed with the reagent in lower chambers 20, reagent bottle 100 is inverted, the reagent in lower chambers 20 passes through half-duplex channel 31
Flow in upper chamber 10, thus the reagent realized in upper chamber 10 is mixed with the reagent in lower chambers 10.
As shown in figure 1, in the present embodiment, half-duplex channel is the through hole 31 being arranged on separator 3, sets in through hole 31
There is a check valve 32, the entrance of check valve 32 faces down chamber 20, and exit face is to upper chamber 10.It is preferred that through hole 31 is arranged at
The middle part of separator 3, and the center of the Kong Xinyu separator 3 of through hole 31 substantially overlaps.
In order that the reagent of bottom chamber can effectively be mixed with the reagent in upper chamber 10, the volume of upper chamber 10 is more than
Or it is equal to the reagent of bottom chamber and the volume sum of the reagent of epicoele interior.The benefit of so setting is, when by reagent bottle 100
It is inverted, so that when the reagent in lower chambers 20 is flowed in upper chamber 10 by half-duplex channel 31, the reagent in lower chambers 20
Can be completely in upper chamber 10.
As a kind of optimal way, the volume V of upper chamber 101Volume V with lower chambers 202Meet following relation：V1/V2
=3/1.
The reagent bottle 200 of second embodiment of the present utility model is described with reference to Fig. 2.Fig. 2 is the reagent of the present embodiment
The structural representation of bottle 200.The present embodiment differs primarily in that half-duplex channel with first embodiment.Other local real with first
Apply that example is identical, relevant portion refer to the statement of first embodiment, will not be described in detail herein.
As shown in Fig. 2 separator 3A is provided with half-duplex channel 31A, half-duplex channel 31A is to be arranged at leading on separator 3A
Hole, (direction that reagent bottle 200 is just being put) reduces the diameter of through hole from top to bottom, thus through hole is the through hole with taper, logical
It is provided with the 31A of hole and cone seal plug 32A, cone seal plug 32A and through hole 31A coordinates, thus when reagent bottle 200 is just put,
Through hole 31A is sealed by cone seal plug 32A, and when reagent bottle is inverted, the reagent in lower chambers 20 can pass through cone seal
Gap between plug 32A and through hole 31A flows in upper chamber 10, thus realizing in reagent and the lower chambers 20 in upper chamber 10
Reagent mixes.
As a kind of optimal way, in the present embodiment, through hole 31A is arranged at the middle part of separator 3A, and through hole 31A
The center of Kong Xinyu separator 3 substantially overlaps, and the volume of upper chamber 10 is more than or equal to reagent and the epicoele of bottom chamber
The capacity sum of indoor reagent.So setting makes reagent bottle 200 be inverted thus the reagent in lower chambers 20 passes through unidirectional leading to
When road 31 flows in upper chamber 10, the reagent in lower chambers 20 can be completely in upper chamber 10.And can be in upper chamber 10
Inside realize being sufficiently mixed of reagent.
The reagent bottle 300 of 3rd embodiment of the present utility model is described with reference to Fig. 3.Fig. 3 is the reagent of the present embodiment
The structural representation of bottle 300.The present embodiment and first and second embodiment differ primarily in that half-duplex channel.Other local with the
First, two embodiments are identical, and relevant portion refer to the statement of first and second embodiment, will not be described in detail herein.
As shown in figure 3, the upper surface of separator 3B is provided with a circle groove 3B1 around all portions of this separator, in groove 3B1
Inside it is provided with multiple through hole 3B2, is provided with sealing ring 3B3 in groove 3B1, when reagent bottle 300 is just put, sealing ring 3B3 is by groove
Through hole 3B2 sealing in 3B1, and when reagent bottle 300 is inverted, the reagent in lower chambers 20 can pass through the through hole of separator 3
3B2 flows in upper chamber 20, thus the reagent realized in upper chamber 10 is mixed with the reagent in lower chambers 20.
Wherein, in order to more preferably realize the effect of half-duplex channel 3B2, groove 3B1 is provided with the conical surface, i.e. the width of groove 3B1
(direction when reagent bottle 300 is just put) is gradually reduced from top to bottom, the form fit of sealing ring 3B3 and groove 3B1, thus when examination
When agent bottle 300 is just put, sealing ring 3B3 carries out tight seal to passage 3B2, and when reagent bottle 300 is inverted, in lower chambers 20
Reagent in the presence of, sealing ring 3B3 slightly moves up, thus the reagent in lower chambers 20 can be flowed into by passage 3B2
Upper chamber, thus mix with the reagent in upper chamber 10.
In three above embodiment, as a kind of optimal way, separator is integrally formed with bottle.
The application of reagent bottle 100 of the present utility model is described with reference to specific embodiment.
Embodiment 1. free thyroxine (FT4) reagent 2
1) reagent bottle lower interlayer is equipped with multiple nonionic surfactants
2) reagent bottle upper interlayer is equipped with free thyroxine enzyme diluent and enzyme conjugates
Nonionic surfactant is mixed for a long time with free thyroxine enzyme diluent and enzyme conjugates and diluent occurs
The situation that middle small amount of albumen separates out, therefore need to separate both；And in the presence of surfactants, can be effectively improved
Precision on different Chemiluminescence Apparatus (remarkable C1800) for the reagent, the content in serum due to free thyroxine at present
Very low, in 0.9-2.2ng/dL about, reagent detection difficulty is larger, and between test, repeatability is larger, is adding non-ionic surface active
After agent, the precision of reagent is greatly improved.Add results contrast before and after nonionic surfactant：
Using the reagent test precision on instrument 1,2,3,4 and 5 respectively not adding surfactant.Calibration object and precision
Degree sample measures the results are shown in Table one.
The different instrument free thyroxine precision measurement result of table one
Result shows, except on instrument 3 FT4 reagent accurate degree preferably in addition to, on instrument 5, result can meet product requirement reluctantly,
On instrument 1,2,4, free thryomimetic agent precision all can not meet product requirement.
Add appropriate nonionic surfactant in reagent, investigate precision on different instruments for the reagent before and after adding
Situation.The results are shown in Table two
The impact to different instrument free thyroxine reagent accurate degree for table two defoamer
From the point of view of result above, add 0.1% nonionic surfactant A, 0.005% nonionic surfactant B
Reagent accurate degree is superior to the precision of original formulation reagent on each instrument.
Below described preferred embodiment of the present utility model in detail, it is understood that reading this utility model
Above-mentioned teachings after, those skilled in the art can make various changes or modifications to this utility model.These shapes of equal value
Formula equally falls within the application appended claims limited range.
Claims (10)
1. a kind of reagent bottle for chemiluminescence immunoassay detection, described reagent bottle accommodates at least two different types of examinations
Agent it is characterised in that described reagent bottle includes bottle and lid, be isolated inside described bottle part be divided into two separate
Upper chamber and lower chambers, described upper chamber and bottom chamber accommodate different reagent respectively, are provided with described separator
Half-duplex channel.
2. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described half-duplex channel
For being arranged at the through hole on described separator, and it is provided with check valve in described through hole, the entrance of described check valve is towards institute
State lower chambers, and the outlet of described check valve is towards described upper chamber.
3. the reagent bottle for chemiluminescence immunoassay detection according to claim 2 is it is characterised in that described through hole is arranged
In the middle part of described separator, and described in the Kong Xinyu of described through hole, the center of separator substantially overlaps.
4. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described upper chamber
Volume is more than or equal to the reagent of described bottom chamber and the capacity sum of the reagent of described epicoele interior.
5. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described upper chamber
Volume V1Volume V with described lower chambers2Meet following relation：V1/V2=3/1.
6. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described half-duplex channel
For being arranged at the through hole on described separator, the diameter of described through hole reduces from top to bottom, thus described through hole is to have taper
Through hole, be provided with described through hole and cone seal plug, described cone seal plug and the cooperation of described through hole.
7. the reagent bottle for chemiluminescence immunoassay detection according to claim 6 is it is characterised in that described through hole is arranged
In the middle part of described separator, and described in the Kong Xinyu of described through hole, the center of separator substantially overlaps, and described upper chamber
Volume be more than or equal to described bottom chamber the reagent reagent indoor with described epicoele capacity sum.
8. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that in described separator
Upper surface form a circle groove around all portion of this separator, be provided with multiple through holes in the bottom of described groove, described recessed
It is provided with sealing ring in groove.
9. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described reagent bottle
Lower chambers equipped with multiple nonionic surfactants, and the upper chamber of described reagent bottle equipped with free thyroxine enzyme dilute
Liquid and enzyme conjugates.
10. the reagent bottle for chemiluminescence immunoassay detection according to claim 1 is it is characterised in that described separator
It is integrally formed with described bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621013681.6U CN205982290U (en) | 2016-08-31 | 2016-08-31 | A reagent bottle for chemiluminescent immunoassay detects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621013681.6U CN205982290U (en) | 2016-08-31 | 2016-08-31 | A reagent bottle for chemiluminescent immunoassay detects |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205982290U true CN205982290U (en) | 2017-02-22 |
Family
ID=58039793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621013681.6U Active CN205982290U (en) | 2016-08-31 | 2016-08-31 | A reagent bottle for chemiluminescent immunoassay detects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205982290U (en) |
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2016
- 2016-08-31 CN CN201621013681.6U patent/CN205982290U/en active Active
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| GR01 | Patent grant |