CN205954012U - Strain preservation tube and strain preservation tube tube cap thereof - Google Patents
Strain preservation tube and strain preservation tube tube cap thereof Download PDFInfo
- Publication number
- CN205954012U CN205954012U CN201620930868.6U CN201620930868U CN205954012U CN 205954012 U CN205954012 U CN 205954012U CN 201620930868 U CN201620930868 U CN 201620930868U CN 205954012 U CN205954012 U CN 205954012U
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- lid
- barrier film
- tube
- strain
- preservative tube
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Abstract
The utility model relates to a strain preservation tube and strain preservation tube tube cap thereof. This strain preservation tube includes the body and locates the tube cap on the body, and the body has the cultivation chamber that is used for culture growth, and the tube cap includes the lid, is equipped with the diaphragm on the lid, and the diaphragm encloses with the lid and becomes to be used for to place protectant chamber that holds, is equipped with on the lid and punctures the mechanism, punctures the mechanism and includes and be used for punctureing and make the preservative that holds in the chamber fall into the head of punctureing of cultivateing the chamber, and punctureing the mechanism and still include drive mechanism, drive mechanism has an input that is used for in strain preservation tube outside operation, and drive mechanism still has to be used for driving and punctures head orientation diaphragm surface and remove and puncture unfamiliar output in order to realize punctureing the head, and the power transmission path of drive mechanism from the input to the output has elasticity and passes power section. When using, the tube cap need not opened to protectant joining, has improved save efficiency, is difficult for causing microbial contamination moreover.
Description
Technical field
The utility model belongs to microbial preservation technique field and in particular to a kind of strain preservative tube and its strain preservative tube
Lid.
Background technology
Plant pathogenetic bacteria and fungi are the important tests materials of microorganism and molecular biology research, separate, purify and obtain
The pathogen obtaining is susceptible to become XOR loss in squamous subculture, activation.Strain variation is had with its metabolic speed
Correlation, low temperature, drying and isolation air etc. are extraneous to adjust the principal element being to reduce bacterial strain metabolic capability.It is logical that bacterial strain preserves
Cross the regulation of these three factors, make bacterial strain be in semidormancy state, and maintain vigour within a certain period of time and biological property, with
It is easy to follow-up research.The store method of bacterial strain is a lot, including Secondary Culture Refrigerator store method, Liquid nitrogen storage method and sharp freezing
Method etc..Secondary Culture Refrigerator store method is without special installation.The process of Secondary Culture Refrigerator store method is as follows：Bacterial strain is trained in liquid
After growing well in foster base, add protective agent such as glycerine, paraffin oil, skim milk etc., put into and can preserve for a long time at subzero 4 DEG C.
Secondary Culture Refrigerator store method is with low cost, simple, is widely used in microorganism preserves.The culture of mushroom and preservation need
Pipe to be preserved.When adding protective agent, need to open lid, then protective agent added in preservation pipe, reduce preservation efficiency,
And opening of lid also can increase the probability that bacterial classification is contaminated.
Utility model content
The purpose of this utility model is to provide a kind of strain preservative tube to solve to add protectant mistake into preservation pipe
The preservation efficiency needing in journey to open lid and causing is low, technical problem that is easily causing strain pollution.Mesh of the present utility model
Also reside in offer a kind of strain preservative tube lid.
In the utility model, the technical scheme of strain preservative tube lid is：A kind of strain preservative tube lid it is characterised in that：
Including lid, lid is provided with barrier film, and barrier film and lid surround for placing protectant accommodating chamber, and lid is provided with puncturing machine
Structure, punctures mechanism and includes for puncturing described barrier film making in the culture chamber that the protective agent in accommodating chamber falls into strain preservative tube
Puncture head, puncture mechanism and also include transmission mechanism, transmission mechanism has the input for operation outside strain preservative tube, pass
Motivation structure also have for drive puncture head towards membrane surface movement to realize puncturing the output end that head punctures barrier film, driver
Drive path from input to output end for the structure has elastic power transmission section.
Lid includes roof and the side wall enclosing located at roof surrounding, and the lower end of side wall is provided with the company for being connected with body
Socket part, barrier film is connected on four the week side of boss walls.
Output end is made up of the button on the roof being connected to lid, and button has elasticity, and elastic power transmission section is by vertically setting
The spring put is constituted, and the upper end of spring is connected on button.
Puncture the lower end that head is connected to spring.
It is provided with protective agent in accommodating chamber.
In the utility model, the technical scheme of strain preservative tube is：A kind of strain preservative tube, including body with located at body
On lid, body there is culture chamber for growth it is characterised in that：Lid includes lid, and lid is provided with barrier film,
Barrier film and lid surround for placing protectant accommodating chamber, and lid is provided with and punctures mechanism, punctures mechanism and includes for puncturing
Described barrier film and make the protective agent in accommodating chamber fall into the head that punctures in culture chamber, puncturing mechanism also includes transmission mechanism, transmission
Mechanism have for outside strain preservative tube operation input, transmission mechanism also have for drive puncture head towards barrier film
To realize puncturing the output end that head punctures barrier film, drive path from input to output end for the transmission mechanism has bullet for surface movement
Property power transmission section.
Lid includes roof and the side wall enclosing located at roof surrounding, and the lower end of side wall is provided with the lid for being connected with body
Body connecting portion, body is provided with the body connecting portion for matching with lid connecting portion, and barrier film is connected on four the week side of boss walls.
Output end has elasticity by the button on the roof being connected to lid, button, and elastic power transmission section is by vertically arranged
Spring is constituted, and the upper end of spring is connected on button.
Puncture the lower end that head is connected to spring.
It is provided with protective agent in accommodating chamber.
The beneficial effects of the utility model are：When using, protective agent is positioned in accommodating chamber, adds in needs are to body
Outside strain preservative tube, when entering protective agent, operate the input of transmission mechanism, transmission mechanism drives and punctures head towards barrier film table
Barrier film is punctured by face movement, and protective agent flows in body from accommodating chamber, and protectant addition does not need to open lid, improves guarantor
Deposit efficiency, and do not easily cause strain pollution.Additionally, the Path of Force Transfer from input to output end has elastic power transmission section, bullet
Property power transmission section enable to act on the power of output end and be more than the power being pre-designed and just puncture barrier film, when using strain preservative tube,
There is maloperation sometimes, as long as the power of maloperation is not reaching to the power being pre-designed, barrier film would not be punctured, therefore elasticity
Power transmission section can avoid to a certain extent because maloperation is by diaphragm breakup.
Brief description
Fig. 1 is the structural representation of the embodiment one of strain preservative tube in the utility model；
Fig. 2 is the structural representation of the embodiment two of strain preservative tube in the utility model.
In figure：1st, body；2nd, lid；3rd, inoculum；4th, label；5th, conduit saddle；6th, lid；7th, barrier film；8th, accommodating chamber；
11st, cutter；12nd, graduation mark；13rd, medium slant；14th, button；15th, spring.
Specific embodiment
Below in conjunction with the accompanying drawings embodiment of the present utility model is described further.
The embodiment one of strain preservative tube of the present utility model, as shown in Fig. 1, strain preservative tube preserves pipe for bacterium.Bacterium
Plant preservation pipe and include body 1 and the lid on body 2.Body 1 has the culture chamber for bacterial growth, in culture chamber
Equipped with sterilized inoculum 3, directly bacterial classification can be linked in culture chamber, allow strain growth.Inoculum can
To be made up of any one in the conventional inoculums such as nutrient broth, LB nutrient solution, EC meat soup.Set on the tube wall of body 1
There is the label 4 of frosted, in order to direct labeled strain information.The lower end of body 1 is provided with conduit saddle 5, reduces when body is collided
Impulsive force and bottom temp change, slow down the impact to bacterial strain for the body vibrations.The tube wall of body 1 is provided with graduation mark 12, convenient
Weigh and add protectant volume.
Lid 2 includes lid 6, and lid includes roof and encloses side wall located at roof surrounding, the lower end of side wall be provided with for
The lid connecting portion being connected with body 1, body is provided with the body connecting portion for matching with lid connecting portion, and lid is even
Socket part is the internal thread on the wall of side, and body connecting portion is the external screw thread on body, and body 1 and lid 2 pass through screw thread
Connect and mutually fix, body 1 and lid 2 connection are provided with the sealing ring for sealing cooperation between body 1 and lid 2.Lid
It is provided with barrier film 7, barrier film 7 is connected on four the week side of boss walls, barrier film and lid surround for placing protectant accommodating chamber 8.Accommodate
Built with sterilized protective agent, protective agent is one of conventional bacterial strain protective agent such as glycerine, paraffin oil, skim milk in chamber 8
Or it is multiple.
Lid is provided with and punctures mechanism, punctures mechanism and includes making for puncturing barrier film the protective agent in accommodating chamber fall into training
Puncture head in foster chamber, puncture mechanism and also include transmission mechanism, transmission mechanism has for operation outside strain preservative tube
Input, transmission mechanism also have for drive puncture head towards membrane surface movement to realize puncturing the output that head punctures barrier film
End, Path of Force Transfer from input to output end for the transmission mechanism has elastic power transmission section.Input is by the roof being connected to lid
On button 14 constitute, button has elasticity, and elastic power transmission section is made up of vertically arranged spring 15, and the upper end of spring is connected to
On button, puncture the cutter 11 that head is the lower end being connected to spring, cutter is the blade of cone, the point of a knife court of cutter divides into
Put.Before adding protective agent in culture chamber, point of a knife is just in the upside of barrier film.
After strain culturing is good, when needing to add protective agent, press down on button, button occurs elastic deformation, passes through
The elastic deformation of button drives spring and cutter to stab barrier film down, and barrier film is punctured, and protective agent flows downwardly in culture chamber,
Protective agent plays a protective role in Cord blood to bacterial strain.
Strain preservative tube in the utility model has advantages below：（1）Body, built with sterilized nutrient solution, reduces
Early stage preparation work, only need to be by inoculation to nutrient solution, raising preservation efficiency.（2）Load in accommodating chamber and sterilize
Protective agent, by pressing the upper button of gland body, drive spring to move downward so that cutter punctures barrier film, protective agent can be to
Under be flowed in culture chamber, addition protective agent of need not uncapping again, reduce bacterial strain pollution probability.（3）Body is led to lid
Cross threaded and connection is provided with sealing ring, make preservation pipe be in sealing state, decrease miscellaneous bacteria and enter in preservation pipe
Possibility.（4）Body is provided with the label of frosted, marking pen record bacterial strain information can be used directly, conveniently write and smear.
（5）The bottom of body is provided with the conduit saddle of plastics, can slow down body and topple over the impact to bacterial strain during vibrations.(6) tube wall of body
It is provided with graduation mark, protectant volume of addition can be specified.
In the utility model, the embodiment two of strain preservative tube is as shown in Fig. 2 strain preservative tube preserves pipe, body for fungi
Inside it is provided with the medium slant 13 for fungi growth, medium slant can be by PDA culture medium, oat medium, carrot training
Any one in the conventional fungi culture mediums such as foster base, V8 culture medium is made.
In the other embodiment of strain preservative tube in the utility model, button 14 also can not possess elasticity, button and lid
Roof slipper seal cooperation.When using, when pressing down on button, button down movement is transported downwards with driving spring and cutter
Move and so that barrier film is punctured.
In the other embodiment of strain preservative tube in the utility model, do not fit in protective agent in accommodating chamber, lid sets
Have for loading protectant load hole in accommodating chamber, load hole is provided with closure, when needing to add protective agent, then beat
Open closure, protective agent is loaded in accommodating chamber by load hole, then close top cover labyrinth, by acting on the defeated of transmission mechanism
Enter end to puncture barrier film, to add protective agent into body.
In the other embodiment of strain preservative tube in the utility model, cutter can be replaced by pin or steel wire etc..
In the other embodiment of strain preservative tube in the utility model, input can from roof slipper seal with bar
Constitute；The lower end of spring also can be connected with rigid section, and cutter is connected to the lower end of rigid section；Elastic power transmission section also can have two sections with
On；Elastic power transmission section can be constituted by having the resilient elastic component of tool such as rubber；Also rigidity can be connected between button and cutter
Section.
In the other embodiment of strain preservative tube in the utility model, button 14 relatively horizontal orientation is obliquely installed in side wall
On, spring is connected on button 14, and the setting direction of spring is consistent with the incline direction of button 14, and button has elasticity, passes through
Pressing button, drives spring to move, the direction that spring mobile band cutter tilts along relatively horizontal orientation stabs barrier film.
In the other embodiment of strain preservative tube in the utility model, barrier film is connected on the side wall of lid, and barrier film is half
Spherical shell shape or the structure of cup shape, on the wall of side, spring is connected on button button, and spring horizontal is arranged, and cutter is connected to
On spring, pressing button drives spring, cutter movement to puncture barrier film.
In the other embodiment of strain preservative tube in the utility model, barrier film is connected on the roof of lid, and barrier film is half
Spherical shell shape or the structure of cup shape, barrier film and roof surround accommodating chamber；Lid can be the vertically extending cylinder of thickness, post
Body lower end is connected with hemisphere hull shape or the barrier film of cup shape structure, and barrier film surrounds accommodating chamber with the lower end of cylinder.
In the embodiment of strain preservative tube lid in the utility model, the structure of strain preservative tube lid and strain preservative tube
Embodiment in lid structure identical, no longer here repeats.
Claims (10)
1. a kind of strain preservative tube lid it is characterised in that：Including lid, lid is provided with barrier film, and barrier film and lid surround use
In placing protectant accommodating chamber, lid is provided with and punctures mechanism, and puncturing mechanism and including for puncturing described barrier film makes receiving
Protective agent in chamber falls into and punctures head in the culture chamber of strain preservative tube, punctures mechanism and also includes transmission mechanism, transmission mechanism
Have for outside strain preservative tube operation input, transmission mechanism also have for drive puncture head towards membrane surface
Move to realize puncturing the output end that head punctures barrier film, drive path from input to output end for the transmission mechanism has elasticity biography
Power section.
2. strain preservative tube lid according to claim 1 it is characterised in that：Lid includes roof and encloses located at roof four
The side wall in week, the lower end of side wall is provided with the connecting portion for being connected with body, and barrier film is connected on four the week side of boss walls.
3. strain preservative tube lid according to claim 2 it is characterised in that：Output end is by the roof being connected to lid
Button constitute, button has elasticity, and elastic power transmission section is made up of vertically arranged spring, and the upper end of spring is connected to button
On.
4. strain preservative tube lid according to claim 3 it is characterised in that：Puncture the lower end that head is connected to spring.
5. the strain preservative tube lid according to claim 1-4 any one it is characterised in that：It is provided with protection in accommodating chamber
Agent.
6. a kind of strain preservative tube, the lid including body and on body, body has the culture chamber for growth,
It is characterized in that：Lid includes lid, and lid is provided with barrier film, and barrier film and lid surround for placing protectant accommodating chamber,
Lid is provided with and punctures mechanism, and puncturing mechanism and including for puncturing described barrier film makes the protective agent in accommodating chamber fall into culture chamber
In puncture head, puncture mechanism and also include transmission mechanism, transmission mechanism have for outside strain preservative tube operation input
End, transmission mechanism also have for drive puncture head towards membrane surface movement to realize puncturing the output end that head punctures barrier film,
Drive path from input to output end for the transmission mechanism has elastic power transmission section.
7. strain preservative tube according to claim 6 it is characterised in that：Lid includes roof and encloses located at roof surrounding
Side wall, the lower end of side wall is provided with the lid connecting portion for being connected with body, body be provided with for lid connecting portion phase
The body connecting portion of cooperation, barrier film is connected on four the week side of boss walls.
8. strain preservative tube according to claim 7 it is characterised in that：Output end by the roof being connected to lid by
Button, button has elasticity, and elastic power transmission section is made up of vertically arranged spring, and the upper end of spring is connected on button.
9. strain preservative tube according to claim 8 it is characterised in that：Puncture the lower end that head is connected to spring.
10. the strain preservative tube according to any one in claim 6-9 it is characterised in that：It is provided with protection in accommodating chamber
Agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620930868.6U CN205954012U (en) | 2016-08-24 | 2016-08-24 | Strain preservation tube and strain preservation tube tube cap thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620930868.6U CN205954012U (en) | 2016-08-24 | 2016-08-24 | Strain preservation tube and strain preservation tube tube cap thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205954012U true CN205954012U (en) | 2017-02-15 |
Family
ID=57977539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620930868.6U Active CN205954012U (en) | 2016-08-24 | 2016-08-24 | Strain preservation tube and strain preservation tube tube cap thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205954012U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107557282A (en) * | 2017-11-01 | 2018-01-09 | 福建省农业科学院植物保护研究所 | A kind of biocontrol bacteria bacteria fermentation reservoir bottle |
| CN111607498A (en) * | 2020-06-02 | 2020-09-01 | 盐城市第一人民医院 | Medical science inspection laboratory is with instrument of opening tub |
-
2016
- 2016-08-24 CN CN201620930868.6U patent/CN205954012U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107557282A (en) * | 2017-11-01 | 2018-01-09 | 福建省农业科学院植物保护研究所 | A kind of biocontrol bacteria bacteria fermentation reservoir bottle |
| CN107557282B (en) * | 2017-11-01 | 2021-01-26 | 福建省农业科学院植物保护研究所 | Storage bottle for bio-control bacterial agent fermentation |
| CN111607498A (en) * | 2020-06-02 | 2020-09-01 | 盐城市第一人民医院 | Medical science inspection laboratory is with instrument of opening tub |
| CN111607498B (en) * | 2020-06-02 | 2023-07-18 | 盐城市第一人民医院 | Medical science inspection laboratory is with instrument of opening a tube |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |