CN205898667U - Device of spot test microorganism outer membrane protein state - Google Patents
Device of spot test microorganism outer membrane protein state Download PDFInfo
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- CN205898667U CN205898667U CN201620571620.5U CN201620571620U CN205898667U CN 205898667 U CN205898667 U CN 205898667U CN 201620571620 U CN201620571620 U CN 201620571620U CN 205898667 U CN205898667 U CN 205898667U
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Abstract
The utility model discloses a device of spot test microorganism outer membrane protein state. The device contains varistor ware, light source, collimating mirror, cell, cell support, total mark ball and fiber optic spectrometer, along the optical axis orientation, the income perforation of light source, collimating mirror, cell support, total mark ball is arranged in proper order, and the income perforation of collimating mirror, cell support and total mark ball is connected for hugging closely, the cell sets up on the cell support, and the width of cell is not less than the diameter of the income perforation of total mark ball, the total mark ball passes through optic fibre and is connected with fiber optic spectrometer, the light source contains deuterium lamp and halogen lamp LED, and the halogen lamp LED is connected with the varistor ware. The utility model discloses the test is quick, realizes second level 100 milliseconds of level data collection even, can test the fast reaction of suspension system, the halogen lamp LED light intensity is adjustable, can change in a flexible way ultraviolet ray and visible light the intensity contrast do not, realize the test of different demands.
Description
Technical field
This utility model belongs to Protein Detection field, particularly to a kind of dress of quick mensure microorganism outer membrane protein state
Put and method.
Background technology
Soil microorganism is the engine of driving element biogeochemical cycle, and the oxidation-reduction process that it drives is contact
Different ring layer materials and the important tie of energy exchange.
1987, American scientist derek lovely first from deposit separate obtain one plant can utilize extracellular
Solid matter is that the microbicidal metal of electron acceptor reduces ground bacillus gs-15 (geobacter metallireducens gs-
15).1988, American scientist ken nealson separation from the deposit in New York oneida lake obtains another strain to be had
The microorganism Xi Washi Oneida mr-1 (shewanella oneidensis mr-1) of same capabilities.This two plants of microorganisms
Find, open the frontier of extracellular breathing.
Extracellular breathing refers under anaerobic condition, and microorganism discharges electronics, the electronics of generation in intracellular exhaustive oxidation Organic substance
It is delivered to extracellular electron acceptor through intracellular respiratory chain, and produce the process that energy maintains own growth.Extracellular microbial external respiration machine
System needs to probe into from protein level.Cytochrome c (c-type in extracellular electron transfer process, on microbial cell film
Cytochromes, c-cyts) it is most important active specy, electronics passes through the phase interaction on cell membrane between cytochrome c
With being transferred to epicyte from inside microbes, thus completing whole redox reaction.In view of about extracellular microbial external respiration
The oxidoreduction of soil Minerals, and mutually can be coupled with the degraded of the Transport And Transformation of heavy metal and organic pollution,
Have great importance in biogeochemical process, the research of cytochrome c becomes very crucial.
In extracellular respiratory, the transmission of electronics is substantially the oxidoreduction between each material, however, active somatic cell pigment c
Redox state be not easy to measure.Conventional molecular biology method, such as rna expression analysis, transcriptome analysis etc.,
The total amount change of cytochrome c all can only be obtained.In view of containing a large amount of heme group in cytochrome c, this group 410～
419 nanometers, 522 nanometers and 550 nanometers have and absorb more by force, can come test cell pigment c's using these absworption peaks
State change.Therefore, cytochrome c is carried out extraction purification by researcher, is characterized in conjunction with its spectral absorption, for extracellular electricity
The research of sub- pass through mechanism.
However, the cytochrome c on cytochrome c after extraction purification and living microorganism adventitia differ greatly it is difficult to
The time of day of reflection microbial function albumen.Test living microorganism cytochrome c, is truly to reflect microbial function albumen
The key of state.But live bacteria is bulky grain, in the solution formed suspension, incident illumination occurs after suspension scatter and
Change direction so that most of transmitted light cannot be introduced into detector it is difficult to the actual transmission light quantity of detection, bring very high background
Absorption errors, common spectrum test cannot be smoothed out.Integrating sphere is that one kind to being in ball or can be placed on outside ball and close
Sample at certain window to scattering of light or a kind of high efficiency device of being collected of transmitting, in recent years, using ultraviolet-can
See that spectrum is connected with integrating sphere it is achieved that capture to scattered light, smoothly deduction scattered light background value, reduces baseline noise,
Scattered light background absorption is solved the problems, such as from methodology, active somatic cell pigment c test is applied.Built purple using integrating sphere
Outward-visible diffuse transmission light spectra system carries out spectrum test to suspension system, and nakamura etc. is obtained using spectrum means first
The redox state change of Shewanella cytochrome c.
However, ultraviolet-visible spectrophotometer carries out during broadband spectrum test being swept it is necessary to constantly adjust wavelength
Retouch, scanning process then needs time enough., scanning speed sets taking the general tu-1901 type spectrophotometer of the general analysis in Beijing as a example
It is set to " fast ", sweep spacing is " 1 nanometer ", from 600 nano scannings to 300 nanometers, takes around 70 seconds.And the micro- life of extracellular breathing
The change of beyond the region of objective existence theca cell pigment c state belongs to a second order reaction.Obviously overflow using the traditional slow that common spectrophotometer is built
Transmitted light spectrometer cannot realize the real-time monitoring of microorganism adventitial cell pigment c state change.On the market, fiber spectrometer can
Rapid data record is carried out to the whole spectrum of 200-1100nm, but although fiber spectrometer has rapid data record
Function, but there is no and the integrating sphere equipment being applied to suspension test associated with fiber spectrometer, ordinary optic fibre spectrogrph is to suspension
When system is tested, it will have very high baseline noise so that test cannot be carried out.
Utility model content
The purpose of this utility model is to overcome the shortcoming of prior art and deficiency, provides outside a kind of microorganism of quick mensure
The device of memebrane protein state.
The purpose of this utility model is achieved through the following technical solutions: a kind of quick mensure microorganism outer membrane protein state
Device, comprises rheostat, light source, collimating mirror, cuvette, cuvette support, integrating sphere and fiber spectrometer；Along optical axis side
To, light source, collimating mirror, cuvette support, the entrance aperture of integrating sphere are arranged successively, wherein, collimating mirror, cuvette support and integration
The entrance aperture of ball is to be close to connection；Cuvette is arranged on cuvette support, and the width of cuvette is not less than entering of integrating sphere
The diameter of perforation, thus the light that light source sends, is after the liquid to be checked in cuvette, could be integrated ball capture completely
Arrive；Integrating sphere is connected with fiber spectrometer by optical fiber；Light source comprises deuterium lamp and Halogen light, and Halogen light is connected with rheostat, leads to
Cross rheostat and adjust light intensity.
Described deuterium lamp preferably spectral region is 190-400nm, power is the deuterium lamp of 100w.
Described Halogen light preferably spectral region is 350-2500nm, power is 10w or the Halogen light more than 10w.
The width of described cuvette is slightly larger than the diameter of the described entrance aperture of integrating sphere.
Described integrating sphere is the integrating sphere that incident bore dia is 9mm.
Described cuvette is cuvette that width is 10mm.
Described cuvette preferably has a cuvette of following structure: on the sealing-plug of cuvette setting injection port and
Outlet, thus the operation in test constantly is more easy.
The diameter of described injection port is preferably 1mm.
The diameter of described outlet is preferably 1mm.
Described integrating sphere is radiation integrating sphere.
Described fiber spectrometer is preferably thermoelectric cooling type fiber spectrometer.
Described thermoelectric cooling type fiber spectrometer is preferably the avaspec-uls2048l thermoelectric cooling type light of avantes
Optical fiber spectrograph.
Described cuvette is to be flexibly connected with the connection of described cuvette support.
Described collimating mirror is arranged between light source and cuvette support, selects collimating mirror to be directly connected to light source and cuvette
Support and do not use optical fiber to connect, decrease the light intensity attenuation between light source and cuvette, reach test suspension system needed for
Light intensity.
Described integrating sphere is close to be connected with described cuvette support, advantageously reduces the loss of scattered light.
A kind of method of quick mensure microorganism outer membrane protein state, is to be realized using said apparatus, comprises the steps:
(1) suspension sample to be detected is positioned in the cuvette being arranged on cuvette support；
(2) light beam is sent by light source, and collimated mirror reaches cuvette support, occurs to dissipate after suspension sample to be detected
Penetrate, scattered light is captured by integrating sphere, the signal of capture is by fiber optic conduction to fiber spectrometer；
(3) the absorption spectrum signal that fiber spectrometer collects is transported to computer, obtains microorganism adventitia egg after analysis
White state.
Described suspension sample to be detected prepares preferably by following steps:
1. microbial bacteria suspension phosphate buffer to be detected is adjusted concentration；
2. bacteria suspension is placed in conical flask, is passed through noble gases and carries out deoxygenation process；
3. making lactic acid sodium solution, is passed through noble gases and carries out deoxygenation process；
4. the sodium lactate solution 3. obtaining the bacteria suspension obtaining after step 2. deoxygenation process and step after deoxygenation is processed mixes
Close, obtain suspension sample to be detected.
Step 1. described in phosphate buffer be ph=6.8～7.2, the phosphate-buffered of 100～200mmol/l
Liquid；It is preferably the phosphate buffer of ph=7,200mmol/l.
Described phosphate buffer is sodium phosphate buffer, i.e. the buffering of disodium hydrogen phosphate and sodium dihydrogen phosphate composition
Liquid.
Step 1. described in concentration be preferably od600=1.0.
Step 2. with step 3. described in noble gases be preferably nitrogen.
Step 2. with step 3. described in deoxygenation process time be more than 20 minutes.
Step 3. described in sodium lactate concentration be preferably 0.5～1.5mol/l；It is preferably 1mol/l.
4. step is preferably: by 3.61ml the step bacteria suspension that obtains and 0.19ml step 3. at deoxygenation after 2. deoxygenation is processed
The sodium lactate solution mixing obtaining after reason, obtains suspension sample to be detected.
Described microorganism is preferably shewanella oneidensis mr-1.
Described microorganism outer membrane protein is cytochrome c.
Described state refers to reducing condition or the state of oxidation.
This utility model has such advantages as with respect to prior art and effect:
This utility model, by being designed light source with integrating sphere, optimizes connected mode, by integrating sphere and suspension colorimetric
Ware test system is connected so that can be carried out in fiber spectrometer using the method for integrating sphere measurement suspension spectral absorption, will
Integrating sphere can obtain the advantage of diffuse transmission light spectrum signal and fiber spectrometer carries out the advantage of rapid data record and combines,
It is successfully applied to the quick test of microorganism suspension system absorption spectrum it is achieved that living microorganism adventitia oxygen in seconds
Change the test of state/reduction-state cytochrome c.This means of testing can be additionally used in the suspension body that microorganism is reacted with soluble substrate
System, carries out original position on-line testing, and the ultraviolet-visible absorption spectroscopy of other suspension chemical reaction system is quickly tested, and has
Larger using value.Concrete analysis is as follows:
First, this utility model can be entered to the scattering of light of the sample being placed on ball outer and at window using integrating sphere
The principle that row is collected, it is achieved that purpose that suspension system absorption spectrum is tested, reaches the baseline back of the body meeting test request
Scape value.As shown in Figure 3.
Second, this utility model achieves the light intensity magnitude needed for integrating sphere measurement suspension liquid light time spectrum, it is achieved thereby that sharp
Possibility with integrating sphere measurement suspension system.
3rd, this utility model is passed through integrating sphere and is combined with fiber spectrometer, compared with traditional slow diffusing transmission spectrum, surveys
Examination is quick, realizes second level even 100 Millisecond gathered datas, the fast reaction of suspension system can be tested.As Fig. 4 institute
Show.100 Milliseconds mean that if only need to test visible region spectrum, device of the present utility model can by adjust
Section Halogen light light intensity, obtains the visible ray of high intensity, after light intensity increases, may be implemented in the faster test of visible region.
4th, in this utility model, Halogen light light intensity is adjustable, can flexibly change the intensity difference of ultraviolet light and visible ray
Not, realize the test of different demands.
Brief description
Fig. 1 is the structural representation quickly measuring the device of microorganism outer membrane protein state；Wherein, 1- rheostat, 2- light
Source, 3- collimating mirror, 4- cuvette support, 5- integrating sphere, 6- optical fiber, 7- fiber spectrometer, 8- integrating sphere incident light hole.
Fig. 2 is the structural representation of improved cuvette；Wherein, 9- cuvette, 10- sealing-plug, 11- injection port, 12-
Outlet.
Fig. 3 is that the background noise analysis fruit of four kinds of devices is schemed.
Fig. 4 is the test result figure to suspension system rapid-action for the traditional slow spectrogrph.
Fig. 5 is this utility model to suspension system rapid-action test result figure.
Fig. 6 is the time dependent result figure of peak value at 552nm in Fig. 4 and Fig. 5, and curve e is to be tested by this utility model
The data point obtaining, curve f is the data point being obtained by the test of traditional slow diffuse transmission light spectrometry.
Specific embodiment
With reference to embodiment and accompanying drawing, this utility model is described in further detail, but enforcement of the present utility model
Mode not limited to this.
Embodiment 1
A kind of device of quick mensure microorganism outer membrane protein state, as shown in figure 1, include rheostat 1, light source 2, collimation
Mirror 3, cuvette, cuvette support 4, integrating sphere 5 and fiber spectrometer 7；Along optical axis direction, light source 2, collimating mirror 3, cuvette
Support 4, integrating sphere entrance aperture 8 are arranged successively, and wherein, collimating mirror 3, cuvette support 4 and integrating sphere entrance aperture 8 are to be close to even
Connect；Cuvette is arranged on cuvette support 4, and integrating sphere entrance aperture 8 is mated with cuvette；Integrating sphere 5 passes through optical fiber 6 and optical fiber
Spectrogrph 7 connects.Deuterium lamp and Halogen light is comprised, wherein Halogen light partly can carry out light intensity regulating by rheostat 1 in light source 2.
Integrating sphere 5 is irradiation type integrating sphere, and its entrance aperture 8 is through transformation, a diameter of 9mm；The width of cuvette is 10mm.Fiber spectrum
Instrument 7 is thermoelectric cooling type fiber spectrometer (avantes company avaspec-uls2048l thermoelectric cooling type fiber spectrometer).
In a kind of preferred scheme, be contrast color ware sealing-plug transformed so as to the operation in test constantly more
For simplicity, specifically as shown in Figure 2: setting injection port 11 and outlet 12 on the sealing-plug 10 of cuvette 9.Injection port 11 straight
Footpath is preferably 1mm, and the diameter of outlet 12 is preferably 1mm.
In a kind of preferred scheme, the deuterium lamp spectral region in light source 2 is 190-400nm, and the spectral region of Halogen light is
350-2500nm, the power of deuterium lamp is 100w and the power of Halogen light is 10w or is more than 10w (scalable).
Embodiment 2
The thalline scattered light background noise of 4 kinds of devices is analyzed: device a is single fiber spectrometer, device b is
(i.e. light source passes through optical fiber with cuvette support (for solubility solution testing to the conventional integrating sphere external member of fiber spectrometer collocation
Support) connect, radiation integrating sphere passes through optical fiber and spectrogrph and connects, cuvette support is arranged on the entrance aperture of radiation integrating sphere
Place), device c is traditional slow diffuse transmission light spectrometer (ultraviolet-uisible spectrophotometer of collocation integrating sphere), and device d is this practicality
The device that new embodiment 1 provides.
Test process is as follows:
(1) by Xi Washi Oneida mr-1shewanella oneidensis mr-1 bacterium to be detected, (Chinese Sea is micro-
Biological inoculum preservation administrative center) the suspension sodium phosphate buffer of ph=7,200mmol/l adjusts concentration to od600=1.0；
(2) bacteria suspension is placed in conical flask, is passed through High Purity Nitrogen and carries out deoxygenation process, duration of ventilation is more than 20 minutes；
(3) prepare the sodium lactate solution of 1mol/l, be passed through High Purity Nitrogen and carry out deoxygenation process, duration of ventilation is more than 20 minutes；
(4) respectively in the cuvette with teflon seal plug for four kinds of devices, the 0.19ml concentration is added to be
The sodium lactate solution of 1mol/l；
(5) in the bacteria suspension 3.61ml after logical nitrogen deoxygenation being added to the cuvette having added sodium lactate solution,
Cover rapidly sealing-plug；
(6) place one minute afterwards, the cuvette that will be equipped with suspension sample to be detected is positioned on cuvette support；
(7) running of each device is as follows:
1. device a: light beam is sent by light source, reaches cuvette support (avantes company cuv-uv/vis colorimetric through optical fiber
Ware support), scatter after suspension sample to be detected, the light after scattering is by fiber optic conduction to fiber spectrometer；Optical fiber light
The absorption spectrum signal that spectrometer collects is transported to computer, obtains microorganism outer membrane protein state after analysis.
2. device b: light beam is sent by light source, reaches cuvette support (avantes company cuv-uv/vis colorimetric through optical fiber
Ware support), scatter after suspension sample to be detected, by radiation integrating sphere (avantes company avasphere-30-
Irrad) capture scattered light, the signal of capture is by fiber optic conduction to fiber spectrometer；The absorption spectrum that fiber spectrometer collects
Signal is transported to computer, obtains microorganism outer membrane protein state after analysis.
3. device c: traditional slow spectrogrph carries out spectral scan, after the completion of scanning, obtains the light absorption value of 300-600nm,
Microorganism outer membrane protein state is obtained after analysis.
4. device d: light beam sends collimated mirror by light source and reaches cuvette support, occurs after suspension sample to be detected
Scattering, captures scattered light by integrating sphere, and the signal of capture is by fiber optic conduction to fiber spectrometer；The suction that fiber spectrometer collects
Receive spectral signal and be transported to computer, after analysis, obtain microorganism outer membrane protein state.
(8) testing result is as shown in figure 3, the thalline scattering background value that wherein device a-d test obtains is corresponding in turn to
Curve a-d it is seen then that a, b two lines thalline scattering background value very high it is impossible to test；D result reaches the effect that c result is similar to, that is,
The thalline scattering background value of this utility model device to test gained reaches the effect similar to traditional slow diffuse transmission light spectrometer, full
Sufficient test request.
Note: in the detection of sample suspension, because suspension is to scattering of light, much unabsorbed scattered light also without
Enter detector, this partly light be mistakenly considered " absorbing light " by spectrogrph, therefore spectrogrph thinks that " absorption value " is very high, that is, bring
Very high background value；And integrating sphere have collected these scattered lights so that spectrogrph has correctly identified the number of " absorbing light ",
Decrease background value, i.e. " thalline scattering background value ".
Embodiment 3
First, use this utility model device to detect microorganism outer membrane protein state, be to be realized using the device of embodiment 1,
Using common cuvette, specifically include following steps:
(1) by Xi Washi Oneida mr-1 shewanella oneidensis mr-1 bacterium to be detected, (Chinese Sea is micro-
Biological inoculum preservation administrative center) the suspension sodium phosphate buffer of ph=7,200mmol/l adjusts concentration to od600=1.0；
(2) bacteria suspension is placed in conical flask, is passed through High Purity Nitrogen and carries out deoxygenation process, duration of ventilation is more than 20 minutes；
(3) prepare the sodium lactate solution of 1mol/l, be passed through High Purity Nitrogen and carry out deoxygenation process, duration of ventilation is more than 20 minutes；
(4) in the cuvette with teflon seal plug, the sodium lactate adding 0.19ml concentration to be 1mol/l is molten
Liquid；
(5) in the bacteria suspension 3.61ml after logical nitrogen deoxygenation being added to the cuvette having added sodium lactate solution,
Cover rapidly sealing-plug；
(6) cuvette that will be equipped with rapidly suspension sample to be detected is positioned on cuvette support；
(7) light beam is sent by light source, and collimated mirror reaches cuvette support, occurs to dissipate after suspension sample to be detected
Penetrate, scattered light is captured by integrating sphere, the signal of capture is by fiber optic conduction to fiber spectrometer；
(8) the absorption spectrum signal that fiber spectrometer collects is transported to computer, obtains microorganism adventitia egg after analysis
White state.
2nd, traditional slow spectrogrph is used to detect microorganism outer membrane protein state
(1) by shewanella oneidensis mr-1 bacteria suspension ph=7 to be detected, the phosphoric acid of 200mmol/l
Sodium buffer adjusts concentration to od600=1.0；
(2) bacteria suspension is placed in conical flask, is passed through High Purity Nitrogen and carries out deoxygenation process, duration of ventilation is more than 20 minutes；
(3) prepare the sodium lactate solution of 1mol/l, be passed through High Purity Nitrogen and carry out deoxygenation process, duration of ventilation is more than 20 minutes；
(4) in the cuvette with teflon seal plug, the sodium lactate adding 0.19ml concentration to be 1mol/l is molten
Liquid；
(5) in the bacteria suspension 3.61ml after logical nitrogen deoxygenation being added to the cuvette having added sodium lactate solution,
Cover rapidly sealing-plug；
(6) cuvette that will be equipped with rapidly suspension sample to be detected is positioned over the cuvette support of traditional slow spectrogrph
On；
(7) traditional slow spectrogrph carries out spectral scan, after the completion of scanning, obtains the light absorption value of 300-600nm, after analysis
Obtain microorganism outer membrane protein state.
3rd, testing result, as shown in figures 4-6: Fig. 4 is the result being obtained using traditional slow spectrogrph, Fig. 5 is use
The result that this utility model device obtains, for peak value at 552nm in Figure 4 and 5, (at 552nm, peak value represents cytochrome c also to Fig. 6
The absorption of ortho states) time dependent situation.It can be seen that, using traditional slow spectrogrph (Fig. 4,6), within 70s, only obtain two
Individual data point；Using this utility model device (Fig. 5,6), smoothly monitor shewanella oneidensis mr- in 24s
Spectrum change situation under sodium lactate existence condition for the 1 adventitial cell pigment c.Conclusion: this utility model is overflow with traditional slow
Transmitted spectrum is compared, and test, quickly it is achieved that the data acquisition of second level, smoothly obtains shewanella oneidensis
The mr-1 fast dynamic processes that adventitial cell pigment c is reduced under sodium lactate existence condition, have for its dynamics research
Significance, and traditional method cannot catch dynamic process.
Embodiment 4
A kind of method of quick mensure microorganism outer membrane protein state, is to be realized using the device of embodiment 1, using transformation
Cuvette afterwards, specifically includes following steps:
(1) by shewanella oneidensis mr-1 bacteria suspension ph=7 to be detected, the phosphoric acid of 200mmol/l
Sodium buffer adjusts concentration to od600=1.0.
(2) bacteria suspension is placed in conical flask, is passed through High Purity Nitrogen and carries out deoxygenation process, duration of ventilation is more than 20 minutes；
(3) prepare the sodium lactate solution of 1mol/l, be passed through High Purity Nitrogen and carry out deoxygenation process, duration of ventilation is more than 20 minutes；
(4) in the bacteria suspension 3.61ml after logical nitrogen deoxygenation being added to the cuvette having added sodium lactate solution,
Cover special sealing-plug；
(5) cuvette being stoppered sealing-plug is positioned on cuvette support；
(6) light beam is sent by light source, and collimated mirror reaches cuvette support, occurs to dissipate after suspension sample to be detected
Penetrate, scattered light is captured by integrating sphere, the signal of capture is by fiber optic conduction to fiber spectrometer；
(7) the absorption spectrum signal that fiber spectrometer collects is transported to computer, obtains microorganism adventitia egg after analysis
White state；
(8) while spectrogrph test constantly, using syringe, in the injection port injection 0.19ml concentration of sealing-plug it is
The sodium lactate solution of 1mol/l, the sample having more overflows in the outlet of sealing-plug, as waste liquid；
(9) spectrogrph continues to monitor microorganism outer membrane protein state, obtains adventitial cell pigment c redox state second level
Situation of change.
Above-described embodiment be this utility model preferably embodiment, but embodiment of the present utility model be not subject to above-mentioned
The restriction of embodiment, other any without departing from the change made under spirit of the present utility model and principle, modify, replace
Generation, combination, simplification, all should be equivalent substitute mode, are included within protection domain of the present utility model.
Claims (8)
1. a kind of device of quick mensure microorganism outer membrane protein state it is characterised in that: comprise rheostat, light source, collimating mirror,
Cuvette, cuvette support, integrating sphere and fiber spectrometer；Along optical axis direction, light source, collimating mirror, cuvette support, integration
The entrance aperture of ball is arranged successively, and wherein, the entrance aperture of collimating mirror, cuvette support and integrating sphere is to be close to connection；Cuvette
It is arranged on cuvette support, the width of cuvette is not less than the diameter of the entrance aperture of integrating sphere；Integrating sphere passes through optical fiber and light
Optical fiber spectrograph connects；Light source comprises deuterium lamp and Halogen light, and Halogen light is connected with rheostat.
2. quick mensure microorganism outer membrane protein state according to claim 1 device it is characterised in that: described deuterium
Lamp for spectral region be 190-400nm, power be 100w deuterium lamp.
3. quick mensure microorganism outer membrane protein state according to claim 1 device it is characterised in that: described halogen
Plain lamp for spectral region be 350-2500nm, power be 10w or more than 10w Halogen light.
4. quick mensure microorganism outer membrane protein state according to claim 1 device it is characterised in that: described long-pending
Bulb separation is radiation integrating sphere.
5. quick mensure microorganism outer membrane protein state according to claim 1 device it is characterised in that: described ratio
Color ware is the cuvette with following structure: setting injection port and outlet on the sealing-plug of cuvette.
6. quick mensure microorganism outer membrane protein state according to claim 1 device it is characterised in that: described light
Optical fiber spectrograph is thermoelectric cooling type fiber spectrometer.
7. quick mensure microorganism outer membrane protein state according to claim 6 device it is characterised in that: described heat
Electric refrigeration mode fiber spectrometer is the avaspec-uls2048l thermoelectric cooling type fiber spectrometer of avantes.
8. the device of the quick mensure microorganism outer membrane protein state according to any one of claim 1～7, its feature exists
In:
Described integrating sphere is the integrating sphere that incident bore dia is 9mm；
Described cuvette is cuvette that width is 10mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620571620.5U CN205898667U (en) | 2016-06-14 | 2016-06-14 | Device of spot test microorganism outer membrane protein state |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110325830A (en) * | 2017-02-23 | 2019-10-11 | 锋翔科技公司 | Integrated irradiating and detecting flow cell for liquid chromatogram |
| CN111103247A (en) * | 2019-12-12 | 2020-05-05 | 中山大学新华学院 | Ultraviolet-visible spectrophotometer |
-
2016
- 2016-06-14 CN CN201620571620.5U patent/CN205898667U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110325830A (en) * | 2017-02-23 | 2019-10-11 | 锋翔科技公司 | Integrated irradiating and detecting flow cell for liquid chromatogram |
| JP2020508451A (en) * | 2017-02-23 | 2020-03-19 | フォセオン テクノロジー， インコーポレイテッドＰｈｏｓｅｏｎ Ｔｅｃｈｎｏｌｏｇｙ， Ｉｎｃ． | Integrated illumination detection flow cell for liquid chromatography |
| JP7071987B2 (en) | 2017-02-23 | 2022-05-19 | フォセオン テクノロジー， インコーポレイテッド | Integrated Illumination Detection Flow Cell for Liquid Chromatography |
| US11828682B2 (en) | 2017-02-23 | 2023-11-28 | Phoseon Technology, Inc. | Integrated illumination-detection flow cell for liquid chromatography |
| CN111103247A (en) * | 2019-12-12 | 2020-05-05 | 中山大学新华学院 | Ultraviolet-visible spectrophotometer |
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