CN1293379C - System and method for portable non-destructive detecting plant pigment in fields - Google Patents
System and method for portable non-destructive detecting plant pigment in fields Download PDFInfo
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- CN1293379C CN1293379C CNB2004100092851A CN200410009285A CN1293379C CN 1293379 C CN1293379 C CN 1293379C CN B2004100092851 A CNB2004100092851 A CN B2004100092851A CN 200410009285 A CN200410009285 A CN 200410009285A CN 1293379 C CN1293379 C CN 1293379C
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Abstract
The present invention relates to a system and a method for the portable non-destructive detection of plant pigment in fields. The system comprises a three-wavelength spectra measuring device where an optical source and a detector are correspondingly arranged in, and a neutral reference sample or a leaf to be measured is placed between the light source and the detector. The light source and the detector are respectively electrically connected with a microcontroller which is electrically connected with a serial port circuit and is respectively electrically connected with a display and a keyboard. The detecting method utilizes detected data I0 and I0 I to work out the transmittance T (T=I/I0) of I0 and I each wavelength detecting light to fresh leaves, and then a stoichiometry algorithm is used for working out contents Chl of plant pigment in the leaves. Compared with a traditional measuring wet chemical method, the present invention improves efficiency by tens of times without generating substances harmful to environment.
Description
Technical field
The present invention relates to the system that a kind of plant pigment detects, particularly relate to the system and method for a kind of portable Non-Destructive Testing field plant pigment.
Background technology
The content of pigment in plant leaf blade (comprising chlorophyll total amount, chlorophyll A, chlorophyll B, carrotene etc.) can reflect situations such as plant growth, nutrition, disease and pest, can advise to the cultivation management of crop in view of the above, especially agricultural development be had significance.As document [1] Yan Yanlu, Liu Xinsheng, " research of chlorophyll measuring method ", Beijing Agricultural University's journal, 8 (2), P53～66, described in 1982: the content of existing crop pigment need be measured with the method for visible spectrum or liquid chromatography, needs before the mensuration method of pigment relevant in the crop leaf with wet-chemical extracted, and then separates or measure, these assay methods not only speed are slow, measure process need and consume reagent, can produce waste liquid, environment is had certain influence.Because this method needs the method for pigment relevant in the crop leaf with wet-chemical extracted before doing measurement, be that determined blade must destroy earlier when pre-service, belong to destructive and measure, thus this method affect crop in the process of field growing, carry out the growth of crop and the diagnosis of nutrition.Another method such as document [2] Yan Yanlu, Dai Wenying, Li Changmin etc. " living body measurement of intact leaf chlorophyll content ", Beijing Agricultural University's journal, 11 (3), P281～287,1985, the living body measurement method of the chlorophyll content of in document 2, being introduced, this method need not the method for pigment relevant in the crop leaf with wet-chemical extracted before doing measurement, use the live body blade to be sample, directly measure the transmitance of live body blade, estimate chlorophyll content in the live body blade, but this method is under the non-existent precondition of hypothesis scattering, the chlorophyll that preliminary way of attempting use 2 wavelength removal background composition is carried out the live body blade is measured, still there is following problem: because the existence of scattering, test result has departed from than ear (Beer Law) law, and measurement is limited in can only be under the thin low concentration situation of blade, and just applying of this method is restricted like this.
Summary of the invention
The objective of the invention is to: when overcoming original method with visible spectrum or liquid chromatography and measuring in the plant leaf blade pigment content, its finding speed is slow, measures the defective that process need consumes reagent, also can produce waste liquid, environment is polluted; The defective that causes crop leaf to destroy during main is in measuring plant leaf blade pigment content; In order to realize the content of the pigment in the crop leaf of Non-Destructive Testing in the field growing process; Thereby providing a kind of utilizes multi-wavelength to proofread and correct scattering, remove the influence of background composition, realizes the system and method for the portable Non-Destructive Testing field plant pigment of the pigment content in the Non-Destructive Testing crop leaf.
The object of the present invention is achieved like this:
The system of portable Non-Destructive Testing provided by the invention field plant pigment, comprise: spectral measurement device, it is characterized in that: also comprise a shell 12, keyboard 8 and LCD 9 are installed on the described shell 12, one battery case 11 in order to lay this machine working battery is set in this shell 12, and installation system circuit board 16; This system circuit board 16 is electrically connected with microcontroller 7 respectively by 3 paths of LEDs driving circuits 14, and battery is electrically connected with described 3 paths of LEDs driving circuits 14; Described microcontroller 7 transmits data by serial port circuit 10, and this microcontroller 7 is electrically connected with described keyboard 8 and described LCD 9 on being installed in described shell 12; Described microcontroller 7 is electrically connected with described light source 5 in the described spectral measurement device; Also be electrically connected with photoelectric detector 6 by prime amplifier 15; Described spectral measurement device has 3 wavelength, comprise: a blade that is used for clamping blade to be measured of being made up of upper arm 2 and underarm 3 presss from both sides 1, the underarm 3 of described blade folder 1 is installed on the described shell 12, in the upper arm 2 of this blade folder described light source 5 or photoelectric detector 6 are set, be provided with on the relative face of the upper arm 2 of described photoelectric detector 6 or described light source 5, described blade folder or underarm 3 with relative position in underarm 3 and have slideway, installation one is used for fixing the slip clamp 4 of neutral reference in this slideway, and both are slidingly matched.
Also comprise a computing machine, microcontroller 7 electrically connects by serial port circuit 10 with computing machine.
In above-mentioned technical scheme, described 3 kinds of wavelength monochromatic sources 5 adopt has 3 kinds of wavelength LED, and its wavelength coverage is 350nm～1100nm; Comprise: single wavelength LED lamp is combined to form, and also can be a composite LED lamp that comprises at least 3 wavelength; And by microcontroller 7 its work of control.
Described system driving circuit can make a block system circuit board with microcontroller, serial port circuit, this system driving circuit provide for light source power supply, control light source work, acquisition testing device detection signal and signal Processing is determined in the blade to be measured, measurement result is shown by display 9; The work of drive circuit board is controlled by keyboard 8, and has serial port circuit 10 and also be convenient to carry out communication with outer computer and be connected; Described microcontroller inside is provided with analog-digital converter (A/D converter).
In above-mentioned technical scheme, described neutral reference is to have the material of same absorbent effect to make the thin slice of (as pottery, teflon etc.) to each wavelength near infrared light, is used for the green strength I of each wavelength light of detection light source
0(3 wavelength are respectively I
01, I
02, I
03) and import microcontroller (7) into.When measuring blade, neutral reference is withdrawed from, change the plant fresh leaves and measure getting each wavelength light intensity I (3 wavelength I through blade
1, I
2, I
3) and import microcontroller (7) into, microcontroller (7) utilizes the I of each wavelength
0, I calculates pigment content.
In above-mentioned technical scheme, described detecting device is a semi-conductor photodetector, is placed in the underarm of blade folder, also can be placed in the blade folder upper arm, in order to detect the green strength I of each wavelength analysis light
0And through the intensity I behind the blade, the signal of detection is sent to microcontroller, and is to be measured in treated definite blade.
In above-mentioned technical scheme, described display is LCD or other display, in order to display analysis result and test parameter.
The method that is used for the plant pigment Non-Destructive Testing provided by the invention comprises: carry out according to the following steps at the field plant pigment nondestructive detection system that is used for of the present invention:
1, at first, start power supply, neutral reference is inserted between light source 5 and the detecting device 6 by mobile slip clamp 4;
2, operation keyboard, make microcontroller 7 control light source 5 timesharing output detection light wavelength in the 3 wave spectrum measurement mechanisms respectively, its wavelength is λ 1=650～700nm, λ 2=730～780nm, detection light is sent in the led light source timesharing of λ 3=940～990nm, received by photoelectric detector 6 after seeing through neutral reference, the green strength that promptly is decided to be 3 wavelength light is respectively I
01, I
02, I
03, this 3 wavelength output light is imported microcontroller 7 through preposition amplification and analog-digital converter;
3, neutral reference is withdrawed from, change plant fresh leaves to be measured; This blade to be measured is placed in the upper arm 2 and underarm 3 clips of described 3 wave spectrum measurement mechanisms, manually opens during mensuration and sandwich that the strength by spring clamps automatically behind the blade; The step of the neutral reference of replication is measured each the wavelength light intensity I that sees through the plant fresh leaves, and 3 wavelength are I respectively
1, I
2, I
3, this output light is imported microcontroller through preposition amplification and analog-digital converter;
4, the data (I of each wavelength of microcontroller utilization detection
0With I) calculate each wavelength and detect the transmitance T (T=I/I of light the plant fresh leaves
0), utilize following multiple linear system of equations (1) to calculate the content Chl of plant pigment in the blade then, the result is shown that by display screen its system's testing process is as shown in Figure 4.
Described system of equations (1):
-Log(T1)＝k
λ1C
Chl+h
λ1C
x+S
-Log(T2)＝k
λ2C
Chl+h
λ2C
x+S
-Log(T3)＝k
λ3C
Chl+h
λ3C
x+S
H wherein
λ 1, h
λ 2, h
λ 3Be the attenuation coefficient of leaf water, h
λ 1Value be 0.7 * 10
-8～1.3 * 10
-8, h
λ 2Value be 0.9 * 10
-8～1.6 * l0
-8, h
λ 3Value be 3 * 10
-8～8 * l0
-8k
λ 1, k
λ 2, k
λ 3Be the chlorophyll attenuation coefficient of blade, k
λ 1Value be 0.07～0.13, k
λ 2Value be 0.004～0.009, k
λ 3Value be 0.003～0.01; S is the scattering error item.
The invention has the advantages that:
The system and method for portable Non-Destructive Testing of the present invention field plant pigment is measured live body blade content of chlorophyll, raises the efficiency tens of times than the wet chemical method that tradition is measured, and does not produce environmentally harmful material; Multi-wavelength has proofreaied and correct diffuse transmission influence and the background composition disturbs, and has solved the accurate measurement in the chlorophyll content relative broad range.Can be widely used in diagnosis, breeding, agricultural production and the scientific research of crop.
L, this method are that field live body blade is directly measured, and need not pluck blade and chemical pre-treatment, do not produce pollution, belong to green assay method.
2, this method is to measure fast, raises the efficiency tens of times than the wet chemical method that tradition is measured.
3, adopt a plurality of wavelength combinations of at least 3, proofreaied and correct diffuse transmission influence and eliminated the interference of background composition.
4, can well eliminate of the influence of field parasitic light to measuring.
5, system adopts low power dissipation design, is suitable for portable.
6, measurement structure adopts the sliding blade folder, and is easy to use.
7, select the neutral reference of solid, solved the problem of light source output intensity and detecting device coupling.
8, adopt Chinese liquid crystal easy to use, and can directly demonstrate pigment content.
9, can give PC with data upload.
Description of drawings
Fig. 1 is that the plant pigment NDT system that is used for of the present invention is formed synoptic diagram (the figure dotted line represents to install parts in the enclosure)
Fig. 2 is that 3 wave spectrum measurement mechanisms in the system of the present invention are formed synoptic diagram
Fig. 3 is a system driving circuit device block diagram of the present invention
Fig. 4 is a system of the present invention testing process block diagram
The drawing explanation
The underarm of 1-blade folder 2-blade folder upper arm 3-blade folder
4-slip clamp 5-light source 6-photoelectric detector
7-microcontroller 8-keyboard 9-display
10-serial port circuit 11-battery case 12-shell
13-blade or neutral reference 14-three paths of LEDs driving circuit 15-prime amplifiers
The 16-system circuit board
Embodiment
With reference to accompanying drawing 1, make the system of a portable Non-Destructive Testing field plant pigment, comprising: a shell 12, is installed in the blade folder 1 that is used for clamping blade to be measured on the shell 12; Described blade folder comprises upper arm 2 and underarm 3 two parts, is oppositely arranged the light source 5 of analyzing usefulness and the photoelectric detector 6 of analyzing usefulness in two arms, and this photoelectric detector is the PIN 2CU30S model bought on the market; Have the slideway (not shown) on its blade folder 1, neutral reference slip clamp 4 has been installed in the slideway, both are slidingly matched; Keyboard 8 and LCD 9 are installed on shell 12 1 walls; Be provided with battery case 11 and system circuit board 16 in the shell 12 in order to lay this machine working battery; Between light source of installing on two arms 5 and photoelectric detector 6, place a blade to be measured or neutral reference during detection.
With reference to accompanying drawing 2 and 3: shown in the circuit system device make a plate, comprise 3 paths of LEDs driving circuits 14, serial port circuit 10, make a drive circuit board 16 with the microcontroller 7 of the band A/D converter of the C8051F005 model of buying on the market, the system that also is solidified with in the microcontroller 7 detects working procedure, and it detects operational flow diagram as shown in Figure 4; Wherein 3 paths of LEDs driving circuits 14 are electrically connected with the microcontroller 7 of C8051F005 model respectively; Microcontroller 7 passes data by serial port circuit 10; Microcontroller 7 is electrically connected with keyboard 8 and LCD 9 on being installed in shell 12; Microcontroller 7 with have 3 kinds of wavelength LED monochromatic sources 5, its wavelength coverage is 350nm～1100nm; (or the composite LED lamp that comprises at least 3 wavelength all can) be electrically connected; Also the prime amplifier 15 by a TLC272 model is electrically connected with PIN 2CU30S photoelectric detector 6, and the battery of installing in the battery case 11 is electrically connected with 3 paths of LEDs driving circuits 14.
Detection light by 3 kinds of wavelength LED outputs of C8051F005 microcontroller 7 controls, as seen this detection light be---the near infrared characteristic light is positioned at above the plant leaf blade as detection light source, PIN 2CU30S photoelectric detector is positioned at below neutral reference or the plant fresh leaves, and each wavelength that is used to detect through behind the neutral reference detects light intensity I
0(3 wavelength I
01, I
02, I
03) or through each wavelength detection light intensity I (3 the wavelength I behind the fresh leaves
1, I
2, I
3).Before measuring blade, neutral reference is inserted between light source and the photoelectric detector, to detect the green strength I of each wavelength light by slip clamp
0(3 wavelength I
01, I
02, I
03), through prime amplifier TLC272 enter microcontroller with the analog-digital converter input end, be converted to digital quantity and import microcontroller into.When measuring blade neutral reference is withdrawed from, change the plant fresh leaves and measure each wavelength light intensity I (3 the wavelength I that see through blade
1, I
2, I
3) and import microcontroller C8051F005 into.Data (the I of each wavelength that the microcontroller utilization detects
0With I) calculate each wavelength and detect the transmitance T (T=I/I of light fresh leaves
0), utilize the stoichiometry algorithm computation to go out the content of plant pigment in the blade then, result of calculation is shown by display screen or transmits data by the serial port circuit.
Use the system of above-mentioned portable Non-Destructive Testing field plant pigment, carry out the field plant leaf blade and comprise measuring chlorophyll content in rape leave, Chinese cabbage leaf, Poplar leaves, the frame beans leaf, its concrete steps are as follows:
1 at first selects led light source 5 with 3 wavelength period characteristic light, be installed in the upper arm of blade folder 1, photoelectric detector 6 is installed in the underarm: this first wavelength period is expressed as 650～700nm with λ 1, second wavelength period is expressed as 730～780nm with λ 2, and the 3rd wavelength period is expressed as 940～990nm with λ 3;
2, start power supply, neutral reference is inserted between light source 5 and the detecting device 6 by mobile slip clamp 4;
3, to control the light source output light wavelength in the multi-wavelength spectrum measurement mechanism respectively be λ 1=650～700nm to microcontroller 7, λ 2=730～780nm, λ 3=940～990nm, when led light source sends detection light respectively, receive by photoelectric detector 6 after seeing through neutral reference, promptly be decided to be the green strength I of each wavelength light
0(3 wavelength I
01, I
02, I
03), through prime amplifier 15 and analog-digital converter, input microcontroller 7;
4, neutral reference is withdrawed from, change plant fresh leaves to be measured; This blade to be measured is placed in the upper arm 2 and underarm 3 clips of described multi-wavelength spectrum measurement mechanism, manually opens during mensuration and sandwich that the strength by spring clamps automatically behind the blade; Measure each wavelength light intensity I (3 the wavelength I that see through blade
1, I
2, I
3) through preposition amplification and analog-digital converter, input microcontroller (7); Its neutral reference is to have the material of same absorbent effect to make to each wavelength near infrared light, manufactures with the thin slice of pottery or teflon, is used for the green strength I of detection light source 5 each wavelength light
0(3 wavelength I
01, I
02, I
03) and import microcontroller 7;
5, microcontroller 7 utilizes the data (I of each wavelength that detects
0With I) calculate each wavelength and detect the transmitance T (T=I/I of light fresh leaves
0), utilize following multiple linear system of equations (1) then, utilize the stoichiometry algorithm computation to go out the content Chl of plant pigment in the blade, the result is shown by display screen.
Described system of equations (1):
-Log(T1)＝k
λ1C
Chl+h
λ1C
x+S
-Log(T2)＝k
λ2C
Chl+h
λ2C
x+S
-Log(T3)＝k
λ3C
Chl+h
λ3C
x+S
H wherein
λ 1, h
λ 2, h
λ 3Be the attenuation coefficient of leaf water, h
λ 1Value be 0.7 * 10
-8～1.3 * 10
-8, h
λ 2Value be 0.9 * 10
-8～1.6 * 10
-8, h
λ 3Value be 3 * 10
-8～8 * 10
-8k
λ 1, k
λ 2, k
λ 3Be the chlorophyll attenuation coefficient of blade, k
λ 1Value be 0.07～0.13, k
λ 2Value be 0.004～0.009, k
λ 3Value be 0.003～0.01; S is the scattering error item.
Following measured result is that the data (I0 of each wavelength and I) of utilizing present embodiment to detect calculate the transmitance T (T=I/I0) of each wavelength detection light to the fresh leaves of rape, Chinese cabbage, willow, frame beans, utilize the stoichiometry algorithm computation to go out the content Chl of plant pigment in the blade then, this measures the mean value that adopts above-mentioned various blades, and test result is as follows:
Measured result: μ g/cm
2
Classical way the present invention
1.75 1.69
5.11 4.71
9.73 10.1
13.1 12.7
16.7 16.9
Claims (7)
1. the system of a portable Non-Destructive Testing field plant pigment, comprise: spectral measurement device, it is characterized in that: also comprise a shell (12), keyboard (8) and LCD (9) are installed on the described shell (12), one battery case (11) in order to lay this machine working battery is set in this shell (12), and installation system circuit board (16); This system circuit board (16) is electrically connected with microcontroller (7) respectively by 3 paths of LEDs driving circuits (14), and battery is electrically connected with described 3 paths of LEDs driving circuits (14); Described microcontroller (7) transmits data by serial port circuit (10), and this microcontroller (7) is electrically connected with described keyboard (8) and described LCD (9) on being installed in described shell (12); Described microcontroller (7) is electrically connected with described light source (5) in the described spectral measurement device; Also be electrically connected with photoelectric detector (6) by prime amplifier (15); Described spectral measurement device has 3 wavelength, comprise: a blade that is used for clamping blade to be measured of being made up of upper arm (2) and underarm (3) presss from both sides (1), the underarm (3) of described blade folder (1) is installed on the described shell (12), in the upper arm (2) of this blade folder described light source (5) or photoelectric detector (6) are set, with relative position in underarm (3) described photoelectric detector (6) or described light source (5) are set, have slideway on the relative face of the upper arm (2) of described blade folder or underarm (3), installation one is used for fixing the slip clamp (4) of neutral reference in this slideway, and both are slidingly matched.
2. by the system of the described portable Non-Destructive Testing of claim 1 field plant pigment, it is characterized in that: also comprise a computing machine, microcontroller (7) electrically connects by serial port circuit (10) with described computing machine.
3. by the system of the described portable Non-Destructive Testing of claim 1 field plant pigment, it is characterized in that: described neutral reference is the thin slice made from the material with same absorbent effect, and this sheeting comprises pottery or teflon.
4. by the system of the described portable Non-Destructive Testing of claim 1 field plant pigment, it is characterized in that: described optical source wavelength scope is 350nm～1100nm; Comprise: single wavelength LED lamp is combined to form, or comprises the composite LED lamp of 3 wavelength.
5. by the system of the described portable Non-Destructive Testing of claim 1 field plant pigment, it is characterized in that: described photoelectric detector is a semi-conductor photodetector.
6. method that application rights requires the system of 1 described portable Non-Destructive Testing field plant pigment to detect is characterized in that may further comprise the steps:
A, at first starts power supply, moves the slip clamp (4) of neutral reference, and neutral reference is inserted between light source (5) and the photoelectric detector (6);
B, microcontroller (7) are controlled light source (5) timesharing output detection light wavelength in the 3 wave spectrum measurement mechanisms respectively, and its wavelength is λ
1=650～700nm, λ
2=730～780nm, λ
3=940～990nm detects the green strength I that is received each wavelength light behind the neutral reference of light transmission by photoelectric detector (6)
01, I
02, I
033 wavelength are through prime amplifier (15) and the inner set A/D analog-digital converter of microcontroller, input microcontroller (7);
C, neutral reference is withdrawed from, change plant fresh leaves to be measured; The blade that this blade to be measured is placed on described 3 wave spectrum measurement mechanisms presss from both sides clamping in (1), and repeating step B measures each the wavelength light intensity I that sees through plant fresh leaves to be measured
1, I
2, I
33 wavelength, the analog-digital converter through preposition amplification and microcontroller inside, input microcontroller (7);
The data I that D, microcontroller (7) utilization are detected
0With I, calculate each wavelength and detect the transmitance T=I/I of light fresh leaves
0, utilize the multiple linear system of equations to calculate the content Chl of plant pigment in the blade then, the result is shown by display screen.
7. the method that detects by the system of the described portable Non-Destructive Testing of claim 7 field plant pigment is characterized in that: described multiple linear system of equations:
-Log(T1)＝k
λ1C
Chl+h
λ1C
x+S
-Log(T2)＝k
λ2C
Chl+h
λ2C
x+S
-Log(T3)＝k
λ3C
Chl+h
λ3C
x+S
H wherein
λ 1, h
λ 2, h
λ 3Be the attenuation coefficient of leaf water, h
λ 1Value be 0.7 * 10
-8～1.3 * 10
-8, h
λ 2Value be 0.9 * 10
-8～1.6 * 10
-8, h
λ 3Value be 3 * 10
-8～8 * 10
-8k
λ 1, k
λ 2, k
λ 3Be the chlorophyll attenuation coefficient of blade, k
λ 1Value be 0.07～0.13, k
λ 2Value be 0.004～0.009, k
λ 3Value be 0.003～0.01; S is the scattering error item.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100092851A CN1293379C (en) | 2004-06-30 | 2004-06-30 | System and method for portable non-destructive detecting plant pigment in fields |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100092851A CN1293379C (en) | 2004-06-30 | 2004-06-30 | System and method for portable non-destructive detecting plant pigment in fields |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1715873A CN1715873A (en) | 2006-01-04 |
| CN1293379C true CN1293379C (en) | 2007-01-03 |
Family
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|---|---|---|---|
| CNB2004100092851A Expired - Fee Related CN1293379C (en) | 2004-06-30 | 2004-06-30 | System and method for portable non-destructive detecting plant pigment in fields |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100429519C (en) * | 2006-11-01 | 2008-10-29 | 中国农业大学 | Method for detecting plant electric signal in green house and special equipment thereof |
| US20150130936A1 (en) | 2013-11-08 | 2015-05-14 | Dow Agrosciences Llc | Crop monitoring system |
| CN104634744B (en) * | 2014-12-19 | 2017-12-01 | 云南省烟草公司昆明市公司 | A kind of Maturity of Tobacco Leaf assay method based on reflectance spectrum |
| CN108287214A (en) * | 2017-12-07 | 2018-07-17 | 广西壮族自治区林业科学研究院 | A kind of clipping Chlorophyll meter measures the auxiliary device of coniferous species chlorophyll content |
| CN109142219B (en) * | 2018-08-06 | 2020-05-19 | 中国科学院长春光学精密机械与物理研究所 | Spectrophotometric multi-wavelength detection device and detection method thereof |
| CN113125313A (en) * | 2021-04-01 | 2021-07-16 | 江苏大学 | Portable fluorescent detection device for fog drop deposition of blades |
| CN114264605A (en) * | 2021-12-20 | 2022-04-01 | 武汉谷丰光电科技有限公司 | Hand-held type crop gas pocket micro-phenotype parameter detection device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4295042A (en) * | 1978-09-18 | 1981-10-13 | Fuji Photo Film Co. Ltd. | Method of and device for measuring chlorophyll of living leaves |
| JPS6093946A (en) * | 1983-10-28 | 1985-05-25 | Minolta Camera Co Ltd | Small chlorophyl meter |
| JPS6449941A (en) * | 1987-08-20 | 1989-02-27 | Minolta Camera Kk | Chlorophyl meter |
| US5424840A (en) * | 1992-07-21 | 1995-06-13 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University | In situ chlorophyl absorption meter |
-
2004
- 2004-06-30 CN CNB2004100092851A patent/CN1293379C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4295042A (en) * | 1978-09-18 | 1981-10-13 | Fuji Photo Film Co. Ltd. | Method of and device for measuring chlorophyll of living leaves |
| JPS6093946A (en) * | 1983-10-28 | 1985-05-25 | Minolta Camera Co Ltd | Small chlorophyl meter |
| JPS6449941A (en) * | 1987-08-20 | 1989-02-27 | Minolta Camera Kk | Chlorophyl meter |
| US5424840A (en) * | 1992-07-21 | 1995-06-13 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University | In situ chlorophyl absorption meter |
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| Publication number | Publication date |
|---|---|
| CN1715873A (en) | 2006-01-04 |
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