CN114264605A - Hand-held type crop gas pocket micro-phenotype parameter detection device - Google Patents
Hand-held type crop gas pocket micro-phenotype parameter detection device Download PDFInfo
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Abstract
The invention discloses a hand-held type crop air hole micro-phenotype parameter detection device. The device realizes nondestructive acquisition of leaf air hole phenotype information and digital integrated data analysis and storage with high portability, high flux and high precision. The invention utilizes the spring fixing mechanism to clamp the area of the blade to be collected, thus realizing the nondestructive detection of the living crop blade; the purpose of collecting clear pore microscopic images is achieved by matching a high-precision microscope with a front surface light source; and (3) extracting related property parameters of the blade gas hole device and the pore from the gas hole image by combining an image processing technology. The method can effectively help stomata researchers to quickly and accurately acquire stomata related characters, and the acquisition of the stomata characters is not the bottleneck of stomata research.
Description
Technical Field
The invention belongs to the field of agricultural automation, and particularly relates to a handheld type crop air hole micro-phenotype parameter detection device.
Background
The stomata are apertures surrounded by specialized epidermal cells, directly influence the strength of plant transpiration and photosynthesis, promote the research of crop stomata, contribute to the observation of crop growth conditions and the analysis of morphology, and are very important for the research of plant stress resistance. The stomata phenotype parameter detection is to acquire stomata property parameters by collecting and analyzing stomata microscopic images, mainly comprises the detection of a stomata device and the measurement of the related properties of stomata pores, for the stomata device, the density and the spatial distribution of the stomata device are relatively concerned, for the stomata pores, the parameters of the length, the width, the opening, the area and the like of the pores are important bases for analyzing the activities of different varieties of stomata, and the parameters are important indexes for evaluating the drought resistance of plants.
In order to realize the measurement of the stomata characters of crops, the traditional stomata research mainly adopts a method of observing by a desktop microscope and a scanning electron microscope, so that the efficiency is low, the time and the labor are consumed, and the destructiveness to individual plants is high due to destructive detection. In recent years, automation and image processing techniques have been widely used, and a batch of mobile electron microscopes are available, such as a computer connected by wire or wirelessly for obtaining microscopic images. At present, microscopic image acquisition equipment on the market all needs to organize the film-making or observe potted plant, and degree of automation is low, wastes time and energy, and volume weight is great, and the appearance design is complicated, inconvenient open air carrying use, and the space consumption rate is high.
The invention designs a handheld crop air hole micro-phenotype parameter detection device, realizes high portability, high flux, high precision and nondestructive measurement of leaf air hole characters, and injects new vitality for research of crop leaf air holes.
The invention content is as follows:
technical problem to be solved
Although acquiring the stomata image is not difficult, the image quality and the acquisition efficiency both meet the requirements, on one hand, a microscopic image needs to be acquired on an uneven living body leaf surface, the image cannot be too fuzzy, and on the other hand, the acquisition efficiency of the image needs to be improved so as to ensure the real-time performance and the value retention performance of stomata data. The invention aims to solve the technical problems that the manual operation steps are reduced on the premise of finishing the acquisition work, the high-portability and high-flux pore microscopic image acquisition is realized, and the phenotypic parameter information of the leaf pore is extracted according to the image.
(II) technical scheme
The invention provides a handheld crop air hole micro-phenotype parameter detection device in order to solve the technical scheme adopted by the technical problem.
A hand-held type crop gas hole micro phenotype parameter detection device for realize that high portability, high flux and high accuracy blade gas hole phenotype information do not harm and obtain and data analysis storage, its characterized in that: the handheld crop air hole micro-phenotype parameter detection device is designed by adopting a flat cuboid machine body, and is convenient for an operator to hold by a palm; the machine body is internally provided with an image acquisition system and an intelligent analysis system, the outer side of the back of the machine body is provided with a blade clamping mechanism and an image acquisition button which are fixedly connected with the machine body and used for fixing and acquiring crop blades to be detected, and one side of the front of the machine body is provided with a display system which is used for displaying the acquired images and measurement results and providing a human-computer interaction interface, so that an operator can conveniently process and analyze the acquired data; the image acquisition system comprises a macroscopic imaging unit, a microscopic imaging unit and a corresponding light source, and is used for respectively acquiring the appearance data of the plant and the high-resolution image of the leaf stomata from a macroscopic scale and a microscopic scale for subsequent image processing and analysis; the intelligent analysis system obtains the stomata phenotype parameters by using an image processing and analyzing means based on the stomata microscopic image obtained by the image acquisition system, so as to realize digital integrated data analysis and storage; the blade clamping mechanism and the machine body are integrally designed, the blade clamping mechanism comprises a blade fixing mechanism and a manual control mechanism, and the blade part required to be collected is flexibly clamped through manual control of an operator.
Preferably, the blade fixing mechanism comprises a fixing arm connected with the manual control mechanism and a glass plate adhered to the back surface of the machine body; a microscopic imaging unit positioned in the machine body is arranged below the glass plate; the fixing arm of the blade fixing mechanism and the glass plate on the surface of the machine body jointly complete the clamping and fixing operation of the blade; the thickness of the glass plate ensures a suitable imaging distance.
Preferably, the manual control mechanism is located on the right side of the back of the machine body, and the image acquisition button is located on the upper right side of the back of the machine body and used for performing acquisition operation.
Preferably, the microscopic imaging unit is positioned at the lower left of the back of the body and is used for acquiring a high-resolution pore microscopic image.
Preferably, the macro imaging unit is positioned at the upper left of the back surface of the machine body and is used for acquiring a macro image of the plant.
Preferably, the display system has a real-time display function, the first viewing angle is microscopic imaging, and the second viewing angle is macroscopic imaging.
Preferably, the intelligent analysis system has an automatic focusing function and is used for acquiring a clear microscopic image of the plant.
Preferably, the imaging light source is positioned above the collecting leaf surface and used for front lighting.
Preferably, the measured pore phenotype parameters include pore density, number of open pores, number of closed pores, pore opening ratio, pore major axis length, pore minor axis length, pore opening, pore average opening, pore area, and pore total area.
(III) advantageous effects
The invention provides a handheld crop stomata micro-phenotype parameter detection device based on technical accumulation and research and development of an inventor in the field for years, and the handheld crop stomata micro-phenotype parameter detection device is used for realizing high portability, high flux and high precision nondestructive acquisition of leaf stomata phenotype information and digital integrated data analysis and storage.
Compared with the prior art, the method has at least the following technical advantages: (1) the design of a flat cuboid machine body is adopted, and the machine body and a clamping part are integrally designed, so that the portable use of operators is facilitated to the maximum extent, plant leaf tissues are not damaged, a leaf collecting area can be fixed, the problem of insufficient space is not considered, the problems of complex treatment of the leaf tissues and low flux collection of a desk microscope required by conventional stomata detection are avoided, and a precondition is provided for high flux collection of stomata images; (2) a high-precision microscope and a front surface light source are matched with each other to obtain a complete and clear pore microscopic image, and a precondition is provided for high-precision acquisition of pore properties; (3) the macro imaging unit and the micro imaging unit are integrated, the defect that the size of the traditional detection equipment is too large is avoided, the software and hardware cost of the equipment is reduced, and therefore the beneficial conditions of equipment popularization are achieved.
Drawings
FIG. 1 is a schematic view of the back side of a hand-held type device for detecting micro-phenotypic parameters of crop stomata according to the present invention
FIG. 2 is a schematic front view of a hand-held device for detecting micro-phenotypic parameters of crop stomata according to the present invention.
Detailed Description
The invention provides a hand-held type crop air hole micro-phenotype parameter detection device, which aims to solve the technical problem.
A hand-held type crop gas hole micro phenotype parameter detection device for realize that high portability, high flux and high accuracy blade gas hole phenotype information do not harm and obtain and data analysis storage, its characterized in that: the handheld crop air hole micro-phenotype parameter detection device is designed by adopting a flat cuboid machine body, and is convenient for an operator to hold by a palm; the machine body is internally provided with an image acquisition system and an intelligent analysis system, the outer side of the back of the machine body is provided with a blade clamping mechanism and an image acquisition button which are fixedly connected with the machine body and used for fixing and acquiring crop blades to be detected, and one side of the front of the machine body is provided with a display system which is used for displaying the acquired images and measurement results and providing a human-computer interaction interface, so that an operator can conveniently process and analyze the acquired data; the image acquisition system comprises a macroscopic imaging unit, a microscopic imaging unit and a corresponding light source, and is used for respectively acquiring the appearance data of the plant and the high-resolution image of the leaf stomata from a macroscopic scale and a microscopic scale for subsequent image processing and analysis; the intelligent analysis system obtains the stomata phenotype parameters by using an image processing and analyzing means based on the stomata microscopic image obtained by the image acquisition system, so as to realize digital integrated data analysis and storage; the blade clamping mechanism and the machine body are integrally designed, the blade clamping mechanism comprises a blade fixing mechanism and a manual control mechanism, and the blade part required to be collected is flexibly clamped through manual control of an operator.
Preferably, the blade fixing mechanism comprises a fixing arm connected with the manual control mechanism and a glass plate adhered to the back surface of the machine body; a microscopic imaging unit positioned in the machine body is arranged below the glass plate; the fixing arm of the blade fixing mechanism and the glass plate on the surface of the machine body jointly complete the clamping and fixing operation of the blade; the thickness of the glass plate ensures a suitable imaging distance.
Preferably, the manual control mechanism is located on the right side of the back of the machine body, and the image acquisition button is located on the upper right side of the back of the machine body and used for performing acquisition operation.
Preferably, the microscopic imaging unit is positioned at the lower left of the back of the body and is used for acquiring a high-resolution pore microscopic image.
Preferably, the macro imaging unit is positioned at the upper left of the back surface of the machine body and is used for acquiring a macro image of the plant.
Preferably, the display system has a real-time display function, the first viewing angle is microscopic imaging, and the second viewing angle is macroscopic imaging.
Preferably, the intelligent analysis system has an automatic focusing function and is used for acquiring a clear microscopic image of the plant.
Preferably, the imaging light source is positioned above the collecting leaf surface and used for front lighting.
Preferably, the measured pore phenotype parameters include pore density, number of open pores, number of closed pores, pore opening ratio, pore major axis length, pore minor axis length, pore opening, pore average opening, pore area, and pore total area.
The working flow of the device is as follows;
the starting equipment clicks account registration, and after corresponding information is filled in, the system automatically creates a data storage folder to which the account information belongs; after the account number login is completed, clicking a microscopic measurement image acquisition window, clamping living plant leaves by using a leaf clamping mechanism, clicking automatic focusing, and after the pore microscopic image is focused, clicking a photographing button at the upper right of the device to finish pore image acquisition; a serial number input window pops up on the human-computer interface, and serial number clicking confirmation is input to create an image serial number; clicking the pore image, and clicking the image for analysis, wherein the detection result can be displayed on the right side of the image; and finally, the measurement result is stored in an Excel table, so that the user can conveniently manage and count the experimental data.
The specific embodiments described in this application are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
Claims (9)
1. A hand-held type crop gas hole micro phenotype parameter detection device for realize that high portability, high flux and high accuracy blade gas hole phenotype information do not harm and obtain and data analysis storage, its characterized in that: the handheld crop air hole micro-phenotype parameter detection device is designed by adopting a flat cuboid machine body, and is convenient for an operator to hold by a palm; the machine body is internally provided with an image acquisition system and an intelligent analysis system, the outer side of the back of the machine body is provided with a blade clamping mechanism and an image acquisition button which are fixedly connected with the machine body and used for fixing and acquiring crop blades to be detected, and one side of the front of the machine body is provided with a display system which is used for displaying the acquired images and measurement results and providing a human-computer interaction interface, so that an operator can conveniently process and analyze the acquired data; the image acquisition system comprises a macroscopic imaging unit, a microscopic imaging unit and a corresponding light source, and is used for respectively acquiring the appearance data of the plant and the high-resolution image of the leaf stomata from a macroscopic scale and a microscopic scale for subsequent image processing and analysis; the intelligent analysis system obtains the stomata phenotype parameters by using an image processing and analyzing means based on the stomata microscopic image obtained by the image acquisition system, so as to realize digital integrated data analysis and storage; the blade clamping mechanism and the machine body are integrally designed, the blade clamping mechanism comprises a blade fixing mechanism and a manual control mechanism, and the blade part required to be collected is flexibly clamped through manual control of an operator.
2. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the blade fixing mechanism comprises a fixing arm connected with the manual control mechanism and a glass plate adhered to the surface of the back of the machine body; a microscopic imaging unit positioned in the machine body is arranged below the glass plate; the fixing arm of the blade fixing mechanism and the glass plate on the surface of the machine body jointly complete the clamping and fixing operation of the blade; the thickness of the glass plate ensures a suitable imaging distance.
3. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the manual control mechanism is located on the right side of the back of the machine body, and the image acquisition button is located on the upper right side of the back of the machine body and used for acquisition operation.
4. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the microscopic imaging unit is positioned at the lower left of the back of the machine body and is used for acquiring a high-resolution pore microscopic image.
5. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the macro imaging unit is positioned at the upper left of the back of the machine body and is used for acquiring a macro image of the plant.
6. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the display system has a real-time display function, the first visual angle is microscopic imaging, and the second visual angle is macroscopic imaging.
7. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the intelligent analysis system has an automatic focusing function and is used for acquiring clear microscopic images of plants.
8. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the imaging light source is located above the collecting leaf surface and used for polishing the front side.
9. The hand-held crop stomata micro-phenotype parameter detection device according to claim 1, characterized in that: the measured pore phenotype parameters include pore density, number of open pores, number of closed pores, pore opening ratio, pore major axis length, pore minor axis length, pore opening, pore average opening, pore area, and pore total area.
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- 2021-12-20 CN CN202111593350.XA patent/CN114264605A/en active Pending
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