CN205676481U - Tissue Culture Dish - Google Patents
Tissue Culture Dish Download PDFInfo
- Publication number
- CN205676481U CN205676481U CN201620544718.1U CN201620544718U CN205676481U CN 205676481 U CN205676481 U CN 205676481U CN 201620544718 U CN201620544718 U CN 201620544718U CN 205676481 U CN205676481 U CN 205676481U
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- set bucket
- culture dish
- hole
- bucket
- cell
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Abstract
The utility model provides a kind of Tissue Culture Dish, it is characterized in that, including: culture dish hole, cultivate cell to be separated, set bucket, is placed in culture dish hole when extracting cell, the cell extraction region of delineation target location, set bucket has three types, respectively: first set bucket, the second set bucket, the 3rd set bucket；There is the first thief hole, the concyclic heart with first set bucket in first set bucket；In second set bucket, there is the second thief hole, deviate with the center of circle of the second set bucket；3rd set bucket has the 3rd thief hole, is positioned at the bucket mural margin of the 3rd set bucket.Tissue Culture Dish of the present utility model, owing to having the set bucket of three types, thief hole position in every kind of set bucket is different, covering the purpose cell of diverse location in culture dish hole, thus improves the purpose of sampling.
Description
Technical field
The utility model relates to a kind of Tissue Culture Dish, belongs to Bioexperiment clothing arts.
Background technology
Cell transfecting is by exogenous molecules such as DNA, and RNA etc. imports the technology of eukaryotic.With molecular biology and thin
The development of born of the same parents' biological study, transfection has become as research and the conventional tool of control gene of eucaryote cell function.Transfection
After 72 hours, transfectional cell is passed on, cultivated by the Selective agar medium containing certain antibiotics.Fail after cultivating through a period of time
The cell of Successful transfection foreign gene is killed, and shows individual cells in culture dish, continues to cultivate visible individual cells division numerous
Grow the single resistant colonies of formation, now can select monoclonal by two kinds of methods:
1. filter paper enzyme: with the dipped pancreatin of 5x5mm filter paper of sterilization, filter paper is attached to 10-15 on single cell colonies
Second, the filter paper that taking-up is stained with cell is put in 24 orifice plates continuation pressurization cultivation.Cell proceeds to after covering with in 24 orifice plates
25cm2In blake bottle, after covering with, proceed to 75cm again2Blake bottle is cultivated.
2. limiting dilution assay: do continuous print 10 times dilution (10-2-10-10) after cell dissociation is got off, by each dilution
The cell of degree is added drop-wise in 96 orifice plates cultivate, and after 7-10 days, selects the hole of single clonal growth to clone again.
Transfection efficiency is low, cannot filter out the stable cell line expressing rotaring redyeing gene is to be frequently encountered by cell transfecting
Main difficult technical.And above two screening technique requires height to experimental technique, and controllability is low.It is as small in method 1 positions
Cell colony and be moved out being very big challenge.Method 2, screening efficiency is low, often occurs acellular in culture hole or has
Multiple clones, and if fail during antibiotic-screening all to kill non-transfected cells, then the method screening success rate greatly
Reduce；If other transfection efficiency is low, above-mentioned contradiction is more prominent, is difficult to filter out the monoclonal cell of transfection.
Utility model content
For the problems referred to above, the purpose of this utility model is to provide newly-designed Tissue Culture Dish to improve screening effect
Rate, improves experiment success rate, shortens experimental period.
The utility model adopts the technical scheme that
A kind of Tissue Culture Dish, it is characterised in that include: culture dish hole, cultivates cell to be separated, overlaps bucket, is extracting
Being placed in during cell in culture dish hole, the cell extraction region of delineation target location, set bucket has three types, respectively: first
Set bucket, the second set bucket, the 3rd set bucket；There is the first thief hole, the concyclic heart with first set bucket in first set bucket；Tool in second set bucket
There is the second thief hole, deviate with the center of circle of the second set bucket；3rd set bucket has the 3rd thief hole, is positioned at the bucket wall of the 3rd set bucket
Edge.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, first set bucket, second
Set bucket and the 3rd set bucket are respectively provided with tweezers gripping piece, are arranged on the edge on set bucket top, for tweezers gripping.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, first set bucket, second
The height of the sidewall of set bucket and the 3rd set bucket is above the height in culture dish hole.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, the end in culture dish hole
There is on face the positioning line that the straight line by concentric circles with through the center of circle is constituted.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, first set bucket, second
The side-wall outer side of set bucket and the 3rd set bucket groove has longitudinal fluting.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, culture dish hole interior
Wall has longitudinal projection, matches with longitudinal fluting.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, the first thief hole,
It at the lower openings of the second thief hole and the 3rd thief hole, is respectively provided with downward projection of edge.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, the end in culture dish hole
Mask has the straight line through the center of circle, and the straight line through the center of circle is corresponding with longitudinal projection.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein projection is distributed in training
Support the bottom in ware hole.
Further, Tissue Culture Dish of the present utility model, can also have a feature in that wherein, first set bucket, second
The area of set bucket and the 3rd set bucket is the 1/8 of the area of culture dish hole bottom surface.
The beneficial effect of utility model
The utility model improves screening efficiency by a kind of newly-designed cell culture container, improves experiment success rate,
Shorten experimental period.If transfection efficiency is low, can be chosen by this device before drug screening after transfection and transfect successfully shared by cell
The cell in the higher region of ratio, raising transfects successfully cell proportion, and (such as transfection efficiency 30%, after this device Preliminary screening
Transfect successfully cell proportion and bring up to 50-80%) after be remarkably improved experiment success rate with antibiotic-screening again.Also can use
This device replaces filter paper enzyme to enter row filter: can be accurately positioned single cell colonies by culture dish bottom surface scale, covers corresponding
Can be accurate, safe after cover plate in cell extraction window by cell extraction out.
Tissue Culture Dish of the present utility model, owing to having the set bucket of three types, the thief hole position in every kind of set bucket
Difference, covering the purpose cell of diverse location in culture dish hole, thus improves the purpose of sampling, and then improves screening
The efficiency of transfectional cell.
Brief description
Fig. 1 is the structural representation in single culture dish hole；
Fig. 2 is the top view in single culture dish hole；
Fig. 3 is the structural representation of first set bucket；
Fig. 4 is the top view of first set bucket；
Fig. 5 is the structural representation of the second set bucket；
Fig. 6 is the top view of the second set bucket；
Fig. 7 is the structural representation of the 3rd set bucket；
Fig. 8 is the top view of the 3rd set bucket；
Fig. 9 is the structural representation in culture dish hole in embodiment two；
Figure 10 is the structural representation of first set bucket in embodiment two；
Figure 11 is the structural representation of the 3rd set bucket in embodiment three.
Detailed description of the invention
Below in conjunction with accompanying drawing, detailed description of the invention of the present utility model is described.
<embodiment one>
Fig. 1 is the structural representation in the single hole of culture dish, and culture dish has plurality of specifications and size, from the number of culture hole
Dividing has 6 holes, 12 holes, 24 holes, 48 holes, 96 hole equal-specifications.Fig. 1 only shows one of them culture dish hole.Such as Fig. 1 and Fig. 2 institute
Show that there is multiple concentric circles 13 and the straight line 12 through the concentric circles center of circle, concentric circles 13 He in the bottom in single culture dish hole 10
Straight line 12 is collectively forming positioning line.As in figure 2 it is shown, concentric circles and cross hairs will be divided into multiple region bottom culture dish, it is assumed that
The area of culture dish hole bottom surface is S, it is assumed that the radius of three concentric circles is respectively a, b, c, then meet following relationship: c=2a,Radius arrange, the area that can make region A is 1/8S, and the area of region B is 1/8S, region C's
Area is 1/16S.Therefore can be by arranging the radius of different concentric circles so that the trizonal area of A, B, C becomes one
Individual specific value so that estimate that the cell quantity in other regions identical with these three region shape is easier.
As shown in Figures 3 to 8, present embodiments provide for three kinds of set buckets, be placed in above-mentioned culture dish hole 10, with individually
Obtain the cell of specific region.
As shown in Figure 3 and Figure 4, there is the first thief hole 21 in the middle of the bottom surface of first set bucket 20, the circle of the first thief hole 21
The heart overlaps with the center of circle of first set bucket 20.The lower openings of the first thief hole 21 is on the bottom surface of first set bucket 20.First set bucket
The external diameter of 20 matches with the internal diameter in culture dish hole 10 so that first set bucket 20 just can be put in culture dish hole 10.When need
During the cell of the center of circle near zone in culture dish hole 10 to be obtained, clamp tweezers gripping piece 22 with tweezers and first set bucket 20 is put into
In culture dish hole 10, the cell in corresponding region therewith is enclosed by the first thief hole 21, forms an independent space, now to
First thief hole 21 adds pancreatin, digests and remove the cell in this region.The bottom surface of first set bucket 20 is transparent coloring
Region, for example blue or purple.Perforate on bottom surface for first thief hole 21 is easily found under the microscope.
As depicted in figs. 1 and 2, the sidewall in culture dish hole has longitudinal projection 11, the sidewall of first set bucket has vertical
To groove 23.Longitudinal projection 11 matches with longitudinal fluting 23.Same, the sidewall of the second set bucket and the 3rd set bucket all has
Longitudinal fluting.Prevent from relatively rotating therebetween after Tissue Culture Dish put into by set bucket, make the position of thief hole fix.
Further, the longitudinal projection 11 on the sidewall in culture dish hole is corresponding with the straight line 12 on the bottom surface of culture dish hole, can
Conveniently position to arrange digital calibration on straight line 12.For example in the range of purpose cell is positioned at place, B region in Fig. 2, B district
Numeral numbering on the straight line of both sides, territory located the position in B region, arranges the number identical with straight line 12 in longitudinal projection 11
Word is numbered.Operating personnel are when operating culture dish and set bucket, and its visual angle tilts under normal circumstances, it is seen that the location comparison side of sidewall
Just.Therefore, when culture dish hole put into by set bucket, operating personnel see that thief hole is carried out by the digital calibration of longitudinal projection 11 position
Pre-determined bit, more under the microscope further by the region at thief hole alignment purpose cell place, further speed up the speed of positioning.
As shown in Figure 5 and Figure 6, the bottom surface of the second set bucket 30, the position in the middle of deviation has the second thief hole 31.Second takes
The center of circle of center of circle deviation the second set bucket 30 in sample hole 31.The sample position correspondence making the second thief hole 31 is positioned at the bottom of culture dish hole
Cell in the range of the circular external concentric circle in face.When at the five-pointed star of the E position that purpose cell is in Fig. 2, can use
Cell herein is sampled by the second set bucket 30.During sampling, clamp tweezers gripping piece 32 with tweezers and the second set bucket 30 is placed in
The top in culture dish hole 10, and rotate the second set bucket 30 and make E position be positioned at the opening range of the second thief hole 31, then put down
Second set bucket 30, carries out follow-up taking cell manipulation.The bottom surface of the second set bucket 30 is transparent painted areas, for example blue or
Purple.Perforate on bottom surface for second thief hole 31 is easily found under the microscope.
As shown in Figure 7 and Figure 8, the edge of the 3rd set bucket 40 has the 3rd thief hole 41, and the 3rd thief hole 41 is fan ring
V notch v, fan annular breach is in addition to contacting with the hole wall of the 3rd set bucket 40, and remaining each limit is respectively provided with raised set
Bucket wall, the 3rd thief hole 41 coordinates with the hole wall in culture dish hole, forms cell sampling region D as shown in Figure 8.When purpose cell
It is positioned at the edge in culture dish hole when the hole wall position in culture dish hole, use the 3rd set bucket 40 to be sampled.During sampling, use tweezer
Sub-folder is lived tweezers gripping piece 42 and second set bucket 30 is placed in the top in culture dish hole 10, and rotates the 3rd set bucket 40 and make purpose cell
It is positioned at the opening range of the 3rd thief hole 41, then puts down the 3rd set bucket 40, carry out follow-up taking cell manipulation.Same,
The bottom surface of the 3rd set bucket 40 is transparent painted areas, for example blue or purple.Make the 3rd thief hole 41 under the microscope
It is easily found.
The perforated area of the first thief hole the 21st, the second thief hole 31 and the 3rd thief hole 41 is equal, is at the bottom of culture dish hole
The 1/8 of the area S in face.
The method using Tissue Culture Dish of the present utility model to carry out cell extraction introduced below.
Step one, determine regional location bottom culture dish hole for the purpose cell；
If the center that step 2 purpose cell is bottom culture dish hole, then select first set bucket, if purpose cell position
In away from culture dish hole bottom centre position, and during the sidewall locations in not up to culture dish hole, select the second set bucket, if purpose
During the position of the sidewall that cell is located close to culture dish hole, select the 3rd set bucket；
Set bucket selected in step 2 is placed in the top in culture dish hole by the nutrient solution in step 3, sucking-off culture dish hole,
Rotary sleeve bucket, until thief hole is positioned at the top of purpose cell compartment, puts down set bucket, makes thief hole delineation region to be sampled；
Step 4, in thief hole, add cell dissociation buffer, the cell in bore region to be sampled is digested get off after, will be thin
Born of the same parents take out, and complete sampling.
<embodiment two>
Essentially identical with embodiment one of culture dish in the present embodiment, difference is: culture dish hole and set
The length of the projection on bucket and groove is different from embodiment one.Present embodiment illustrates as a example by first set bucket, other
Set chimb arranges consistent with first set bucket with projection.
As shown in Figure 9 and Figure 10, the longitudinal projection 51 on culture dish hole 50 is not completely through hole wall, say, that
The hole wall top of culture hole 50 does not has bulge-structure.Longitudinal groove 61 up/down perforation bucket wall on first set bucket 60, or only
Being distributed in the latter half of set bucket, such setting makes when overlapping the top that culture dish 50 put into by bucket 60, due to the weight of the two
Folded part does not has bulge-structure, and the two can relatively rotate, and it is right to remain able to after set bucket 60 is put into the top in culture dish hole
The position of thief hole positions.Meanwhile, after the top in culture dish hole is put in the bottom of set bucket 60, culture dish hole can also be played
Stably overlap bucket effect, make set bucket 60 be sampled hole positioning process more stable.When culture dish put into completely by first set bucket 60
After in 50, projection 51 and groove 61 partly overlap, and prevent from relatively rotating between set bucket and culture dish hole.
Based on the difference on said structure, the cell sampling method of present embodiment is also different from embodiment one.Specifically
As follows:
The utility model also provides a kind of method of cell sampling, it is characterised in that comprise the steps:
Step one, determine regional location bottom culture dish hole for the purpose cell；
If the center that step 2 purpose cell is bottom culture dish hole, then select first set bucket, if purpose cell position
In away from culture dish hole bottom centre position, and during the sidewall locations in not up to culture dish hole, select the second set bucket, if purpose
During the position of the sidewall that cell is located close to culture dish hole, select the 3rd set bucket；
Culture dish hole is put in the lower end of the set bucket selected in step 2 by the nutrient solution in step 3, sucking-off culture dish hole,
Rotary sleeve bucket so that thief hole is positioned at the top of purpose cell compartment, puts down set bucket, makes thief hole delineation region to be sampled；
Step 4, in thief hole, add cell dissociation buffer, the cell in bore region to be sampled is digested get off after, will be thin
Born of the same parents take out, and complete sampling.
<embodiment three>
Culture dish in present embodiment and set bucket can use embodiment one or the structure implementing in two.
Present embodiment is with the difference of the first two embodiment, the first thief hole, the second thief hole and the 3rd sampling
Having the edge of a circle projection at the lower ending opening in hole, as a example by the 3rd set bucket 40, as shown in figure 11, the 3rd thief hole 41 is downward
Extend the edge 43 of projection.The edge action of the projection of three thief holes is to play a supportive role, and prevents from overlapping the bottom damage of bucket
Cell beyond thief hole.This circle projection can be separated choosing cell compartment and other regions simultaneously.Prevent cell dissociation buffer
Or cell spills.
<embodiment four>
It in present embodiment, is with the difference of above-mentioned embodiment, cancel tweezers gripping piece, instead increase set
The Sidewall Height of bucket, makes the sidewall of set bucket exceed culture dish hole one segment distance, and tweezers so more convenient to use grip.
Claims (10)
1. a Tissue Culture Dish, it is characterised in that include:
Culture dish hole, cultivates cell to be separated,
Set bucket, is placed in described culture dish hole when extracting cell, the cell extraction region of delineation target location,
Described set bucket has three types, respectively:
First set bucket, the second set bucket, the 3rd set bucket；
There is the first thief hole, the concyclic heart with described first set bucket in first set bucket；
In second set bucket, there is the second thief hole, deviate with the center of circle of described second set bucket；
3rd set bucket has the 3rd thief hole, is positioned at the bucket mural margin of the 3rd set bucket.
2. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, described first set bucket, described second set bucket and described 3rd set bucket are respectively provided with tweezers gripping piece, are arranged on set bucket
The edge in portion, for tweezers gripping.
3. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, the height of the sidewall of described first set bucket, described second set bucket and described 3rd set bucket is above described training
Support the height in ware hole.
4. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, the bottom surface in described culture dish hole has the positioning line that the straight line by concentric circles with through the center of circle is constituted.
5. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, the side-wall outer side of described first set bucket, described second set bucket and described 3rd set bucket groove has longitudinal fluting.
6. Tissue Culture Dish as claimed in claim 5, it is characterised in that:
Wherein, the inwall in described culture dish hole has longitudinal projection, matches with described longitudinal fluting.
7. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, it at the lower openings of the first thief hole, the second thief hole and the 3rd thief hole, is respectively provided with downward projection of limit
Edge.
8. Tissue Culture Dish as claimed in claim 6, it is characterised in that:
Wherein, the bottom surface in described culture dish hole has a straight line through the center of circle, the described straight line through the center of circle with described longitudinal
Projection is corresponding.
9. Tissue Culture Dish as claimed in claim 6, it is characterised in that:
Wherein, described projection is distributed in the bottom in described culture dish hole.
10. Tissue Culture Dish as claimed in claim 1, it is characterised in that:
Wherein, the area of described first set bucket, described second set bucket and described 3rd set bucket is the area of culture dish hole bottom surface
1/8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620544718.1U CN205676481U (en) | 2016-06-06 | 2016-06-06 | Tissue Culture Dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620544718.1U CN205676481U (en) | 2016-06-06 | 2016-06-06 | Tissue Culture Dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205676481U true CN205676481U (en) | 2016-11-09 |
Family
ID=57434854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620544718.1U Withdrawn - After Issue CN205676481U (en) | 2016-06-06 | 2016-06-06 | Tissue Culture Dish |
Country Status (1)
| Country | Link |
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| CN (1) | CN205676481U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838605A (en) * | 2016-06-06 | 2016-08-10 | 唐明 | Cell culture dish and cell sampling method thereof |
-
2016
- 2016-06-06 CN CN201620544718.1U patent/CN205676481U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838605A (en) * | 2016-06-06 | 2016-08-10 | 唐明 | Cell culture dish and cell sampling method thereof |
| CN105838605B (en) * | 2016-06-06 | 2018-05-11 | 唐明 | Tissue Culture Dish and its cell sampling method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20161109 Effective date of abandoning: 20180511 |
|
| AV01 | Patent right actively abandoned |