CN205658253U - Cooling cooling chamber is preserved to biomaterial low temperature - Google Patents
Cooling cooling chamber is preserved to biomaterial low temperature Download PDFInfo
- Publication number
- CN205658253U CN205658253U CN201620512786.XU CN201620512786U CN205658253U CN 205658253 U CN205658253 U CN 205658253U CN 201620512786 U CN201620512786 U CN 201620512786U CN 205658253 U CN205658253 U CN 205658253U
- Authority
- CN
- China
- Prior art keywords
- inner cavity
- liquid nitrogen
- biomaterial
- rectangular inner
- cooling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model relates to the field of medical equipment, cooling cooling chamber is preserved to biomaterial low temperature especially. Inhomogeneous to the injection of existing equipment liquid nitrogen, the different positions of the cooling chamber inner chamber difference in temperature is big, the distribution of temperature is unbalanced, and cooling rate is difficult to the adjustment, and cause the cell damage, reduce biomaterial survival rate scheduling problem, the utility model provides a cooling cooling chamber is preserved to biomaterial low temperature, including the box of taking the rectangle inner chamber, open the sealed case lid of depositing the mouth with forming a complete set that has with rectangle inner chamber intercommunication on the box wall, set up ring pipe and the reflecting curtain of belt wheel around distribution liquid nitrogen jet orifice in the cooling chamber inner chamber, let the liquid nitrogen spray in the cooling chamber inner chamber evenly, it is balanced to make cooling chamber inner chamber distribution of temperature, and can be according to different biomaterial's characteristic cooling rate adjustment cooling rate, make different biomaterial according to the cooling of its characteristic cooling rate extremely 80 DEG C, make the biomaterial degree of safety cross low temperature preserve in the most important low temperature process.
Description
Technical field
This utility model relates to medical instruments field, particularly biomaterial cryopreservation cooling down room.
Background technology
The preservation of biomaterial such as bone marrow, stem cell, skin, embryo or even organ has been achieved for bigger progress, shadow
Ring the principal element of tissue and organ transplantation success rate in addition to implantation technique, in addition it is also necessary to the biological donor that vitality is strong, improve
The survival rate of biomaterial cryopreservation is significant for operation transplantation.Owing to low temperature can suppress intracellular new old generation
Thank, reduce tissue and the damage of cell, but low temperature can produce dehydrating phenomena, change the concentration parameter of salt and other metabolite
Deng, so biological cell can be caused damage in temperature-fall period, modern age, the research of cryobiology showed, low temperature injury is main
Occurring at 0 DEG C～-80 DEG C of this section of warm areas is " dangerous warm area ", and when biomaterial arrives-80 DEG C of temperature below, low temperature injury is just
It is greatly reduced.No matter the cryopreservation of which kind of biomaterial is required for experiencing three processes, i.e. temperature-fall period, low temperature storage process
And rewarming resuscitation process, wherein temperature-fall period is exactly that from room temperature, biomaterial is down to-80 DEG C with certain rate of temperature fall, often
Planting biomaterial and all there is its feature velocity of suitably lowering the temperature, if meeting this cooling feature velocity, then biological cell is in fall
Damage minimum during temperature；Low temperature storage process is exactly the biomaterial by reaching-80 DEG C of temperature below, is placed into lower
Ambient storage, in theory, the temperature of low temperature storage is the lowest more good, but typically stores under liquid nitrogen temperature,
Disclosure satisfy that the requirement of low temperature storage；Rewarming process is exactly that rewarming recovery is to use in addition by the biomaterial of low temperature storage, multiple
Needing to prevent the generation of recrystallization during temperature, the formation of recrystallization can produce major injury to biological cell, so rewarming rate needs
Strictly to be controlled.By the physics that each process is occurred, chemical change and biological cell may be made in practice
The various factors becoming damage is controlled by, and just can be greatly improved the survival rate of biomaterial, in three mistakes of aforementioned cryopreservation
Cheng Zhong, temperature-fall period is maximum to the survival rate of biological cell, by biomaterial by its feature rate of temperature fall the most safely
Spend " dangerous warm area ", be the key of cryopreservation.Liquid in the inner chamber of existing biomaterial cryopreservation cooling down equipment
Nitrogen injection is uneven, and the temperature difference causing cooling chamber inner chamber diverse location is big, and inner chamber temperature distributing disproportionation weighs, and rate of temperature fall is difficult simultaneously
To adjust, these factors all can cause cell injury, reduces the survival rate of biomaterial.
Utility model content
The technical assignment of the technical problems to be solved in the utility model and proposition is to overcome existing biomaterial cryopreservation
Cooling down equipment inner chamber temperature distributing disproportionation is even and rate of temperature fall is difficult to the technological deficiencies such as regulation, it is provided that biomaterial low temperature is protected
Deposit cooling down room, in described cooling chamber inner chamber liquid nitrogen spray uniformly, the equilibrium of cooling chamber inner chamber Temperature Distribution, it is possible to according to not
Adjust rate of temperature fall with the feature rate of temperature fall of biomaterial, make different biomaterial be cooled to-80 by its feature rate of temperature fall
DEG C, degree of safety crosses the most important chilling process of cryopreservation.
This utility model adopts the following technical scheme that: biomaterial cryopreservation cooling down room, including in band rectangle
The casing in chamber, described box body wall has connect with rectangular inner cavity deposit mouth, deposit mouth and be provided with supporting sealed platform cover, described
Rectangular inner cavity sets liquid nitrogen spraying device, it is characterized in that described liquid nitrogen spraying device is the ring pipe connecing liquid nitrogen supply source, described ring
Shape pipe is located at rectangular inner cavity minor face side and connects computer control jet pressures, and ring pipe is offered cincture along its length
The liquid nitrogen spray hole of distribution, described liquid nitrogen spray hole sprays liquid nitrogen, the rectangle of far-end towards the rectangular inner cavity minor face side of far-end
Inner chamber minor face side is provided with reflecting curtain.This utility model is upper around the liquid nitrogen spray hole being distributed by arranging ring pipe and its,
Liquid nitrogen can uniformly be sprayed ringwise in rectangular inner cavity, it is achieved the low temperature environment of rectangular inner cavity equilibrium, it is possible to pass through
Computer controls the jet pressures of input liquid nitrogen, and then controls liquid nitrogen spray hole ejection liquid nitrogen speed, regulates rate of temperature fall, meets
The feature rate of temperature fall of different biomaterials needs；The effect of reflecting curtain is that the liquid nitrogen avoiding ejection is in rectangular inner cavity side office
Portion gathers, and after the liquid nitrogen of ejection encounters reflecting curtain, buffering is distributed in the middle part of rectangular inner cavity, and liquid nitrogen spreads after buffering, makes square
Rate of temperature fall uniformity in shape inner chamber.
As improving further and supplementing technique scheme, this utility model uses following technical measures: described
Reflecting curtain forms one and dehisces towards ring pipe.Reflecting curtain forms one and dehisces, more preferable, favorably to the buffering effect of liquid nitrogen
In keeping uniform consistent low temperature environment in rectangular inner cavity.
The described angle dehisced is 120 °.The angle dehisced is 120 ° can fully buffer the liquid nitrogen spraying to reflecting curtain,
Making to keep uniform low temperature environment in rectangular inner cavity, angle is excessive does not has the buffering effect to liquid nitrogen, and the too small meeting of angle makes liquid
Nitrogen is collected in dehiscing and cannot spread.
Grate opening is had on described reflecting curtain.The effect of grate opening is to allow liquid nitrogen can enter into reflection through grate opening
The intracavity space at baffle plate rear, allows liquid nitrogen fully spread in cooling chamber inner chamber, it is to avoid become dead angle at this, keeps inner chamber consistent
Low temperature environment.Further, grate opening can make the grating pattern of shutter.
It is provided with between the rectangular inner cavity wall of described ring pipe and adjacent side by motor-driven centrifugal fan, described centrifugal wind
Fan is dried to the rectangular inner cavity wall of far-end from rear, liquid nitrogen spray hole, and motor connects computer and controls rotating speed.Centrifugal fan leads to
Cross motor to drive, dry to the rectangular inner cavity wall of far-end from rear, liquid nitrogen spray hole, blow wind action and ring pipe is sprayed
Liquid nitrogen is fully atomized, and makes rectangular inner cavity cooling uniformly；Motor is connected with computer, can be by changing rotational speed regulation centrifugal fan
Airflow effects.
Described rectangular inner cavity is provided with ring pipe just to and the electrical heating ring of matching form, described ring pipe is positioned at electricity and adds
Between hot ring and centrifugal fan, and electrical heating ring connects computer and controls to heat up.When rate of temperature fall reaches to set speed, because
Also may proceed to spray liquid nitrogen in inertia ring pipe, now open electrical heating ring and carry out auxiliary heating, the liquid nitrogen persistently sprayed is encountered
Electrical heating ring, cushioning balance heat, make the rate of temperature fall of rectangular inner cavity be maintained at the feature rate of temperature fall of setting, will not be because of ring
Shape pipe continues ejection liquid nitrogen and persistently increases.
Being provided with multiple resistance thermometer in described rectangular inner cavity, described resistance thermometer is distributed along rectangular inner cavity long side direction
And be connected with computer.Resistance temperature is calculated as multiple and is all connected with computer, and effect is multiple by measuring in rectangular inner cavity
The low temperature of position, calculates the mean temperature of rectangular inner cavity with computer, to control the jet pressures of liquid nitrogen in OK range, and then
The rate of temperature fall of regulation rectangular inner cavity.
The parallel distance of 15cm～20cm is kept between the rectangular inner cavity wall of described ring pipe and adjacent side.Ring pipe and neighbour
Keep suitable parallel distance between the rectangular inner cavity wall of nearside, reserve installing space, it is possible to for subsequent installation miscellaneous part
Reserve suitable space, such as centrifugal fan etc..
The aperture in described liquid nitrogen spray hole is 1mm.The aperture of spray-hole is excessive or too small all can cause liquid nitrogen spouting velocity
Improper, make liquid nitrogen distribution uniform not, make the bad control of rectangular inner cavity rate of temperature fall, use 1mm aperture injection liquid nitrogen, liquid nitrogen
Can be more evenly distributed in rectangular inner cavity, be beneficial to regulate the rate of temperature fall of control rectangle inner chamber.
Described box body wall is three-decker, including outer wall and the corrosion resistant plate of inwall and be filled in thermal insulation between the two
Layer.The inner and outer wall of casing all uses 304 medical stainless steel plates, convenient cleaning to keep cleaning；Middle heat insulation layer can be adopted
Making with polystyrene material or other expanded material with good thermo-insulation properties, effect is adiabatic, to keep cabinets cavity
Low temperature environment.
This utility model, by arranging ring pipe and on it around the liquid nitrogen spray hole of distribution, enables liquid nitrogen to exist ringwise
Rectangular inner cavity uniformly sprays, it is achieved the uniform low temperature environment of rectangular inner cavity, it is possible to control input liquid nitrogen by computer
Jet pressures, and then control liquid nitrogen spray hole ejection liquid nitrogen speed, regulate rate of temperature fall, meet the feature fall of different biomaterial
Temperature speed needs；The effect of reflecting curtain is that the liquid nitrogen avoiding ejection touches at rectangular inner cavity side local cluster, the liquid nitrogen of ejection
After reflecting curtain, buffering is distributed in the middle part of rectangular inner cavity, and liquid nitrogen spreads after buffering, makes the rate of temperature fall in rectangular inner cavity
Uniformity.
Accompanying drawing explanation
Fig. 1: this utility model schematic appearance.
Fig. 2: this utility model sectional view.
Fig. 3: this utility model ring pipe schematic diagram.
Fig. 4: this utility model reflecting curtain schematic diagram.
In figure: 1. casing, 2. rectangular inner cavity, 3. case lid, 4. ring pipe, 5. liquid nitrogen spray hole, 6. reflecting curtain, 7.
Mouth, 8. grate opening, 9. centrifugal fan, 10. electrical heating ring, 11. resistance thermometers, 12. corrosion resistant plates, 13. heat insulation layers, 14. lifes
Thing material.
Detailed description of the invention
This utility model is described further by explanation and detailed description of the invention below in conjunction with the accompanying drawings.
As shown in figures 1-4, biomaterial cryopreservation cooling down room, including the casing 1 of band rectangular inner cavity 2, described case
Body 1 wall is that three-decker, outer wall and inwall are corrosion resistant plate 12, between the two filled polystyrene material or other have good
The heat insulation layer 13 that the expanded material of good heat-insulating property is made；Have on described casing 1 wall and deposit mouth with rectangular inner cavity 2 connects,
Depositing mouth and be provided with supporting sealed platform cover 3, set liquid nitrogen spraying device in described rectangular inner cavity 2, described liquid nitrogen spraying device is for connecing
The ring pipe 4 of liquid nitrogen supply source, described ring pipe 4 is located at rectangular inner cavity 2 minor face side and connects computer control jet pressures, ring
Parallel distance L between rectangular inner cavity 2 wall of shape pipe 4 and adjacent side is 18cm, and ring pipe 4 is offered cincture along its length
The liquid nitrogen spray hole 5 of distribution, the aperture in liquid nitrogen spray hole 5 is 1mm, and described liquid nitrogen spray hole 5 is short towards the rectangular inner cavity 2 of far-end
Side, limit ejection liquid nitrogen, the rectangular inner cavity 2 minor face side of far-end is provided with reflecting curtain 6, and described reflecting curtain 6 forms one and dehisces
7 towards ring pipe 4, dehisce 7 angle be 120 °, described reflecting curtain 6 has grate opening 8；Described ring pipe 4 and adjacent side
Rectangular inner cavity 2 wall between be provided with by motor-driven centrifugal fan 9, described centrifugal fan 9 from rear, liquid nitrogen spray hole 5 to far
The rectangular inner cavity 2 wall blowing of end, and motor connection computer control rotating speed；It is the most right with ring pipe 4 to be provided with in described rectangular inner cavity 2
And the electrical heating ring 10 of matching form, described ring pipe 4 is between electrical heating ring 10 and centrifugal fan 9, and electrical heating ring 10
Connecting computer to control to heat up, be provided with five resistance thermometers 11 in rectangular inner cavity 2, five resistance thermometers 11 are along rectangular inner cavity
2 long side direction distributions are also connected with computer.
During use, inputting liquid nitrogen to ring pipe in advance, cooling chamber inner chamber hydrojet nitrogen reduces temperature, then opens case lid, will
Biomaterial 14 is put in rectangular inner cavity, covers case lid and seals, continues hydrojet nitrogen, and liquid nitrogen uniformly sprays from the liquid nitrogen spray hole of cincture
Go out, it is achieved the uniform low temperature environment of rectangular inner cavity, the jet pressures of input liquid nitrogen can be controlled by computer, and then control liquid
Nitrogen spray-hole ejection liquid nitrogen speed, regulates rate of temperature fall, meets the feature rate of temperature fall needs of different biomaterial；It is centrifuged simultaneously
Fan rotates, and dries to the rectangular inner cavity wall of far-end from rear, liquid nitrogen spray hole, is fully atomized by the liquid nitrogen of ejection, in making rectangle
Chamber cooling is uniformly；After the liquid nitrogen of ejection encounters reflecting curtain, buffering is distributed in the middle part of rectangular inner cavity, and liquid nitrogen spreads after buffering,
Make the rate of temperature fall uniformity in rectangular inner cavity.Along with the temperature of rectangular inner cavity reduces, when rate of temperature fall reaches to set speed
Time, to open electrical heating ring and carry out auxiliary heating, the liquid nitrogen persistently sprayed encounters electrical heating ring, and cushioning balance heat, in making rectangle
The rate of temperature fall in chamber is maintained at the feature rate of temperature fall of setting, persistently will not increase because ring pipe continues ejection liquid nitrogen.
Claims (10)
1. biomaterial cryopreservation cooling down room, including the casing (1) of band rectangular inner cavity (2), described casing (1) wall is opened
Having and deposit mouth with rectangular inner cavity (2) connects, deposit mouth and be provided with supporting sealed platform cover (3), described rectangular inner cavity sets liquid in (2)
Nitrogen sprayer unit, is characterized in that described liquid nitrogen spraying device is the ring pipe (4) connecing liquid nitrogen supply source, and described ring pipe (4) is located at
Rectangular inner cavity (2) minor face side also connects computer control jet pressures, ring pipe (4) is offered along its length around dividing
The liquid nitrogen spray hole (5) of cloth, described liquid nitrogen spray hole (5) sprays liquid nitrogen, far-end towards rectangular inner cavity (2) the minor face side of far-end
Rectangular inner cavity (2) minor face side be provided with reflecting curtain (6).
Biomaterial cryopreservation cooling down room the most according to claim 1, is characterized in that described reflecting curtain (6) shape
Become one dehisce (7) towards ring pipe (4).
Biomaterial cryopreservation cooling down room the most according to claim 2, it is characterized in that described in dehisce the angle of (7)
It it is 120 °.
Biomaterial cryopreservation cooling down room the most according to claim 2, is characterized in that on described reflecting curtain (6)
Have grate opening (8).
Biomaterial cryopreservation cooling down room the most according to claim 1, is characterized in that described ring pipe (4) and neighbour
Being provided with by motor-driven centrifugal fan (9) between rectangular inner cavity (2) wall of nearside, described centrifugal fan (9) is from liquid nitrogen spray
Dry to rectangular inner cavity (2) wall of far-end in hole (5) rear, and motor connects computer and controls rotating speed.
Biomaterial cryopreservation cooling down room the most according to claim 5, is characterized in that in described rectangular inner cavity (2)
Be provided with ring pipe (4) just to and the electrical heating ring (10) of matching form, described ring pipe (4) be positioned at electrical heating ring (10) and from
Between heart-wind syndrome fan (9), and electrical heating ring (10) connects computer and controls to heat up.
Biomaterial cryopreservation cooling down room the most according to claim 1, is characterized in that in described rectangular inner cavity (2)
Being provided with multiple resistance thermometer (11), described resistance thermometer (11) is along the distribution of rectangular inner cavity (2) long side direction and computer
It is connected.
8., according to the biomaterial cryopreservation cooling down room one of claim 1 to 7 Suo Shu, it is characterized in that described ring pipe
(4) parallel distance of 15cm～20cm and is kept between rectangular inner cavity (2) wall of adjacent side.
9., according to the biomaterial cryopreservation cooling down room one of claim 1 to 7 Suo Shu, it is characterized in that described liquid nitrogen sprays
The aperture of perforation (5) is 1mm.
10., according to the biomaterial cryopreservation cooling down room one of claim 1 to 7 Suo Shu, it is characterized in that described casing
(1) wall is three-decker, including the corrosion resistant plate (12) of outer wall and inwall and be filled in heat insulation layer (13) between the two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620512786.XU CN205658253U (en) | 2016-05-31 | 2016-05-31 | Cooling cooling chamber is preserved to biomaterial low temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620512786.XU CN205658253U (en) | 2016-05-31 | 2016-05-31 | Cooling cooling chamber is preserved to biomaterial low temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205658253U true CN205658253U (en) | 2016-10-26 |
Family
ID=57157799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620512786.XU Expired - Fee Related CN205658253U (en) | 2016-05-31 | 2016-05-31 | Cooling cooling chamber is preserved to biomaterial low temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205658253U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110193335A (en) * | 2019-05-27 | 2019-09-03 | 衢州市沃德仪器有限公司 | A kind of high/low temperature reacting furnace |
| CN110683214A (en) * | 2019-09-03 | 2020-01-14 | 威尚生物技术（合肥）有限公司 | Reagent that can lower temperature keeps box |
| CN111203191A (en) * | 2020-01-16 | 2020-05-29 | 中南林业科技大学 | Preparation method of graphene oxide-doped cellulose adsorption material |
| CN113970941A (en) * | 2020-07-22 | 2022-01-25 | 青岛海尔生物医疗科技有限公司 | Program cooling instrument and cooling method |
-
2016
- 2016-05-31 CN CN201620512786.XU patent/CN205658253U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110193335A (en) * | 2019-05-27 | 2019-09-03 | 衢州市沃德仪器有限公司 | A kind of high/low temperature reacting furnace |
| CN110683214A (en) * | 2019-09-03 | 2020-01-14 | 威尚生物技术（合肥）有限公司 | Reagent that can lower temperature keeps box |
| CN111203191A (en) * | 2020-01-16 | 2020-05-29 | 中南林业科技大学 | Preparation method of graphene oxide-doped cellulose adsorption material |
| CN113970941A (en) * | 2020-07-22 | 2022-01-25 | 青岛海尔生物医疗科技有限公司 | Program cooling instrument and cooling method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205658253U (en) | Cooling cooling chamber is preserved to biomaterial low temperature | |
| JP5174521B2 (en) | Frozen bread dough thawing device | |
| CN102788474B (en) | Fruit and vegetable box applicable to air cooling refrigerator and repaid cooling and moisturizing method of fruit and vegetable box | |
| ES2833441T3 (en) | Thawing or tempering facility for frozen food products | |
| CN204421462U (en) | A kind of refrigerator | |
| CN205694459U (en) | A kind of edible mushroom cultivating device | |
| CN207193288U (en) | A kind of electric heating incubator | |
| KR101790519B1 (en) | Humidification apparatus for dough conditioner fermenter | |
| CN208402677U (en) | A kind of phytoclimate case having the soil moisture and air themperature division control type | |
| CN109416211A (en) | The cooling freezer of multiple pipe | |
| KR101334962B1 (en) | Heating device for ripening doughnut dough material | |
| CN206776397U (en) | A kind of new spatial plant sugar-free tissue culture device | |
| CN108657641A (en) | A kind of food insulation box with moisture-keeping functions | |
| CN211831791U (en) | Seed germination device | |
| CN207744653U (en) | A kind of novel proofing box | |
| CN208554236U (en) | High-low temperature test chamber with heat insulation layer | |
| CN106359171A (en) | Temperature adjustable egg hatching device | |
| CN209660068U (en) | A kind of transport device of flower forcing peony | |
| CN218042814U (en) | Temperature control device for peach tree greenhouse cultivation | |
| CN208165698U (en) | A kind of food insulation box with moisture-keeping functions | |
| WO2021023918A1 (en) | An aeroponic farming system and a method | |
| CN219762095U (en) | Auxiliary assembly for cultivation box | |
| CN105674684B (en) | A kind of control method of refrigerator temperature | |
| KR200426734Y1 (en) | An apparatus for drying agricultural and marine products | |
| CN218195920U (en) | Concrete curing box test block rack |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161026 Termination date: 20190531 |