CN205656122U - Centreless optical fiber sensor of survey streptavidin concentration - Google Patents
Centreless optical fiber sensor of survey streptavidin concentration Download PDFInfo
- Publication number
- CN205656122U CN205656122U CN201620421638.7U CN201620421638U CN205656122U CN 205656122 U CN205656122 U CN 205656122U CN 201620421638 U CN201620421638 U CN 201620421638U CN 205656122 U CN205656122 U CN 205656122U
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- CN
- China
- Prior art keywords
- streptavidin
- fibre
- concentration
- coreless fiber
- optical fibre
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model relates to a centreless optical fiber sensor of survey streptavidin concentration will import optic fibre, no core fibre and output optical fibre in proper order with constituting optical fiber sensor after the optical fiber splicer melting connection, follow smooth behind the sensor that input optic fibre, no core fibre, output optical fibre constitute that broadband light source sent, can detect the transmission interference spectrum on the spectrum appearance, the streptavidin solution of different concentration can change transmission interference spectrum attenuation peak intensity level on being used in the centreless fiber cladding that plates the biotin membrane, through the survey of measuring attenuation peak intensity level realization to streptavidin concentration. This centreless optical fiber sensor has simple structure, the measurement is convenient, sensitivity is high, ann congruent advantage.
Description
Technical field
This utility model relates to Streptavidin concentration determination techniques, optical fiber sensing technology, utilizes and is plating life
On the coreless fiber covering of thing element film, the solution of streptavidin of variable concentrations can make transmission interference spectrum attenuation peak strong
The method that angle value changes, has simple in construction, observes the advantages such as convenient, highly sensitive, safe, it
Belong to fiber-optic biosensor field.
Background technology
Having the strongest specific adsorption ability between Streptavidin and biotin, affinity constant can reach
1015M, is therefore often used as the material of immunology detection, miscellaneous to DNA molecular to the mensuration of Streptavidin
Hand over and prepare single strand dna and have critically important meaning.In common immunology detection technology, immunity
Fluorescent technique needs fluorescent label sample, and instrument and equipment is the most expensive；Radioactivity in Placenta function
Isotope is big to person damage；In Enzyme-linked Immunosorbent Assay technology, background noise is big, brings the most tired to detection
Difficult.These some shortcomings greatly limit Streptavidin application in immunology.
Utility model content
The purpose of this utility model is contemplated to overcome the problem of above-mentioned generation, proposes one mensuration strepto-affine
The coreless fiber sensor of element concentration.Native system simple in construction, reasonable in design, highly sensitive, measurement convenience,
Off the beaten track detection method, application prospect is broad.
For achieving the above object, the technical solution adopted in the utility model is: a kind of mensuration Streptavidin concentration
Coreless fiber sensor, be made up of input optical fibre, coreless fiber and output optical fibre；This utility model utilizes chain
Mould Avidin and the strong feature of biotin specific adsorption, modify with silane coupler on coreless fiber covering
Method plates biotin film, when then the solution of streptavidin of variable concentrations acts on biotin film, leniently
It is delivered to spectrogrph with light source light out through the sensor of input optical fibre-coreless fiber-output optical fibre detect
Transmission interference attenuation peak, interferes attenuation peak intensity level just can reach to measure Streptavidin concentration by detection
Purpose.
The cladding diameter of input optical fibre described in the utility model and output optical fibre is 125 μm, and core diameter is
8μm。
The cladding diameter of coreless fiber described in the utility model is 125 μm, and utilizes silane coupler at covering
In modification, concentration is the biotin film of 5%, and it has specific adsorption binding ability with Streptavidin to be measured.
The transmission interference spectrum that the spectral region of spectrogrph described in the utility model comprises Fibre Optical Sensor generation declines
Subtracting peak intensity value, spectral resolution is 0.01dB.
This utility model have the feature that advantage is: 1. all appts material is the most universal, simple in construction, system
Make conveniently, with low cost.2. Streptavidin is without fluorescent label, straight to the mensuration of Streptavidin
Connect in real time.3. experimentation is without reference to hazardous chemical, safety easily operation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model
Fig. 2 is the schematic diagram that in this utility model, biotin film is fixed on coreless fiber covering
Fig. 3 is this utility model experimental result picture
Detailed description of the invention
Below in conjunction with the accompanying drawings enforcement step of the present utility model is illustrated:
As it is shown in figure 1, it is the structural representation of a kind of coreless fiber sensor measuring Streptavidin concentration
Figure, the light sent from wideband light source (1) is through input optical fibre (2), coreless fiber (3), output optical fibre (4)
After the sensor constituted, spectrogrph (5) can detect transmission interference spectrum；The strepto-parent that will have configured
Acting on successively by concentration order from low to high with cellulose solution has used silane coupler method of modifying fixing raw
On the coreless fiber covering (3) of thing element, the attenuation peak of transmission interference spectrum can be detected from spectrogrph (5)
Intensity level, just can measure the dense of solution of streptavidin by the attenuation peak intensity level of detection transmission interference spectrum
Degree.
As in figure 2 it is shown, it is biotin film in a kind of coreless fiber sensor measuring Streptavidin concentration
It is fixed on the schematic diagram of coreless fiber covering, first with silane coupler (6) at the covering table of coreless fiber
Face is modified the biotin film (7) that upper concentration is 5% and is detected the sensing membrane layer of Streptavidin the most, when difference is dense
When the solution of streptavidin (8) of degree acts on biotin film (7), due to Streptavidin and biotin
The feature of specific adsorption binding ability, can make the attenuation peak intensity level of transmission spectrum change, and different is saturating
Penetrate the concentration of the corresponding Streptavidin of spectrum attenuation peak intensity level, such that it is able to reach the purpose measured.
As it is shown on figure 3, it is the experimental result picture obtained at laboratory with Fig. 1 structure and parameter, use chain
As a example by the concentration of mould Avidin solution is 0M, 0.01M, 0.05M, 0.10M, it is seen that interference spectrum is decayed
Peak intensity value there occurs movement, therefore just can measure strepto-by detection interference spectrum attenuation peak intensity level affine
The concentration of cellulose solution.
Those skilled in the art know clearly, according to method of the present utility model, can enter optical fiber parameter
Row resets, and the components and parts in structure can be in optimized selection, and protection domain of the present utility model is not
It is confined to above example.
Claims (4)
1. the coreless fiber sensor measuring Streptavidin concentration, it is characterised in that by input optical fibre (2),
Coreless fiber (3) and output optical fibre (4) constitute Fibre Optical Sensor after connecting with optical fiber splicer is melted successively；
The light sent from wideband light source (1) is through input optical fibre (2), coreless fiber (3), output optical fibre (4) structure
After the sensor become, spectrogrph (5) can detect transmission interference spectrum；The strepto-of variable concentrations is affine
Cellulose solution acts on to have plated and can change transmission interference spectrum decay on coreless fiber (3) covering of biotin film
Peak intensity value, realizes the mensuration to Streptavidin concentration by detection attenuation peak intensity level.
A kind of coreless fiber sensor measuring Streptavidin concentration the most according to claim 1, its feature
Being: the cladding diameter of described input optical fibre (2) and output optical fibre (4) is 125 μm, core diameter is 8 μm.
A kind of coreless fiber sensor measuring Streptavidin concentration the most according to claim 1, its feature
It is: the cladding diameter of described coreless fiber (3) is 125 μm, and utilizes silane coupler on covering is modified
Concentration is the biotin film of 5%, and it has specific adsorption binding ability with Streptavidin to be measured.
A kind of coreless fiber sensor measuring Streptavidin concentration the most according to claim 1, its feature
It is: the spectral region of described spectrogrph (5) comprises the transmission interference spectrum attenuation peak intensity that Fibre Optical Sensor produces
Value, spectral resolution is 0.01dB.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620421638.7U CN205656122U (en) | 2016-05-06 | 2016-05-06 | Centreless optical fiber sensor of survey streptavidin concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620421638.7U CN205656122U (en) | 2016-05-06 | 2016-05-06 | Centreless optical fiber sensor of survey streptavidin concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205656122U true CN205656122U (en) | 2016-10-19 |
Family
ID=57402174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620421638.7U Expired - Fee Related CN205656122U (en) | 2016-05-06 | 2016-05-06 | Centreless optical fiber sensor of survey streptavidin concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205656122U (en) |
-
2016
- 2016-05-06 CN CN201620421638.7U patent/CN205656122U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161019 Termination date: 20170506 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |