CN103994978A - Label-free light reflection interference optical fiber biosensor - Google Patents
Label-free light reflection interference optical fiber biosensor Download PDFInfo
- Publication number
- CN103994978A CN103994978A CN201410247857.3A CN201410247857A CN103994978A CN 103994978 A CN103994978 A CN 103994978A CN 201410247857 A CN201410247857 A CN 201410247857A CN 103994978 A CN103994978 A CN 103994978A
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- light
- layer
- detection
- interference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a label-free light reflection interference optical fiber biosensor which belongs to the technical field of biochemical equipment. The label-free light reflection interference optical fiber biosensor is characterized in that a full-wave band halogen tungsten lamp is used as a light source, and broadband light is coupled in an optical fiber through a lens collimator; a Y-shaped optical fiber coupler, a source optical fiber connected with a light source, a transmission optical fiber connected with an optical fiber probe and a detection optical fiber connected with a rear beam splitting system are adopted; the optical fiber probe is composed of a multi-mode optical fiber, and the far end of the optical fiber probe is provided with three layers, namely a high-refraction index interference layer, a chemical reagent modified layer and a biomolecule layer, and incident light is subjected to two reflection on the high-refraction index interface layer and the biomolecule layer at the end face; the rear beam splitting system comprises an incident slit, a concave mirror, an optical grating and an array receiver, and the detection on a to-be-tested signal is realized through a signal acquirer. The label-free light reflection interference optical fiber biosensor has the beneficial effects that a sample does not need to be marked and purified based on a light interference principle and a principle of interaction among biomolecule, and the stability and the accuracy of detection are ensured; no other reagents are required to be added during the detection; a dynamic process can be directly detected in real time and in place, and the detection on existence of a to-be-detected material can be simultaneously realized; the label-free light reflection interference optical fiber biosensor is suitable for measurement of a sample on the spot; the man-machine interaction is realized, and the operation is simple.
Description
Technical field
The invention belongs to biochemical equipment technical field, relate to the detecting instrument of the sample to be tested in the fields such as a kind of biology, medical science, agricultural, food.
Background technology
Optical fiber biosensor is using optical fiber as conductive medium and collect signal and detect, and can adapt to extreme environment, is not subject to electromagnetic interference (EMI), corrosion-resistant, signal transmission safety, and loss is low, has non-destruction and the feature such as easy to use and is subject to extensive concern.In optical fiber biosensor, most widely used is biologic sensor for fast travelling waves of optical fibre, when utilizing light wave to transmit in the mode of total reflection, this sensor produces evanescent wave at fibre-optical probe place in optical fiber, the biological substance that is marked with fluorescence molecule connecting on this evanescent wave actuated sensor detecting head surface, produce fluorescence signal, thereby realize the concentration determination that has that it's too late of test substance.But evanescent wave field energy is little, the fluorescence signal inspiring is faint; Detected sample needs fluorescence labeling, but fluorescence labeling poor stability; Evanescent wave not only can detecting sensor surface, can also be deep in test sample solution, and the stability of transducing signal is produced to huge interference.
Summary of the invention
The object of the invention is: a kind of non-marked light reflection interference optical fiber biosensor is provided, it can realize original position, quick, sensitive detection of biological interaction of molecules parameter, and biomolecule and little molecule have or not, antibody and other protein, DNA, micromolecular concentration etc.
Technical scheme of the present invention is:
By the analysis of interference curve being detected to the kinetic parameter of the having or not of biomolecule in sample to be tested, concentration and bio-molecular interaction.This optical fiber biosensor comprises light source, optically coupled system, fibre-optical probe, rear beam splitting system, sample load carrier and six part compositions of control system.
Adopt all band halogen tungsten lamp as light source, broadband is optically coupled into optical fiber by scioptics collimating apparatus;
" Y " type of employing fiber coupler, and the source optical fiber being connected with light source, the Transmission Fibers being connected with fibre-optical probe and the detection fiber being connected with rear beam splitting system;
Fibre-optical probe is made up of multimode optical fiber, three layers of far-end punishment: 1, high index of refraction interfering layer; 2, chemical reagent decorative layer, it has bio-compatibility; 3, bio-molecule layer.There are two secondary reflections by the high refractive index layer at end face and bio-molecule layer in incident light, the interference light reflecting to form for twice is beam splitting system after y-type optical fiber one end enters;
Rear beam splitting system is made up of entrance slit, concave mirror, grating and array receiver, the light that light source sends shines directly into fibre-optical probe surface after collimation, after being irradiated to after collimation converges, reflected light in beam-splitting optical system, realizes detection to measured signal by signal acquisition device;
The major function of sample load carrier is in experimentation, for placing sample to be tested, makes sample to be tested in homogeneous state by certain mechanism simultaneously;
The Main Function of embedded control system is each wave band interference light intensity information of beam-splitting optical system output after resolving, and obtains the required indices of test item, as detection thing to be checked has or not and combination/dissociation rate etc.
Man-machine interactive system is made up of display screen and printer, realizes man-machine interaction, and user can arrange key parameter, and the operation of being correlated with according to the prompting on screen; Observation test flow process, show state curve, by printer output examining report.
Fiber type is multimode optical fiber core, core diameter 400 μ m, and wave band is 200-1100nm, three ends are all standard bindiny mechanism.
The invention has the beneficial effects as follows:
1, utilize the interaction principle between interference of light principle and biomolecule, invent a kind of non-marked light reflection interference optical fiber biosensor, sample, without mark and purifying, has ensured the stability and the accuracy that detect.
2, while detection, without adding other reagent, directly add sample to detect, required sample size is little, processes simply, and response is fast, easily realizes robotization.
3, dynamic process can be detected directly, in real time, in situ, the detection of the having or not of material to be detected, concentration and bio-molecular interaction parameter can be realized simultaneously.
4, volume is little, lightweight, is easy to carry, and is applicable at the scene sample be measured.
5, adopt touch-screen to carry out man-machine interaction, operate simpler; Measurement result can be passed through printer real time print.
6, can realize unit and measure, without connecting PC.
Brief description of the drawings
Fig. 1 is unit construction principle block diagram;
Fig. 2 is light source coupling optical path figure;
Fig. 3 is system light path schematic diagram;
Fig. 4 is optical fiber probe far-end schematic diagram;
Fig. 5 is rear beam splitting system figure;
Fig. 6 is control system block diagram.
Embodiment
It is a kind of interaction principle utilizing between interference of light principle and biomolecule, the fibre-optical probe surface of being fixed on optical fiber sensing system with the part of biomolecule complementation to be measured is formed to biostrome, when adding in reaction tank after such biomolecule, the thickness of fibre-optical probe surface biological layer will produce change, in the time thering is the biostrome of visible ray incident optical probe of certain bandwidth, according to the simplified model of film interference and light reflection refraction law, incident ray is divided into two parts on biostrome surface, form Part I reflected light, enter the transmissive portion of biostrome in second generation of interfaces reflection of biostrome, form Part II reflected light, when light beam vertical incidence, two parts reflected light forms interference wave, detected by spectrometer, interact while generation, biostrome thickness increases, reflected light interference spectrum curve can entirety move to wavelength growing direction, in the time there is combination or dissociation process in interacting molecule, capital causes the drift of interference curve.
By the analysis of interference curve being detected to the kinetic parameter of the having or not of biomolecule in sample to be tested, concentration and bio-molecular interaction.This optical fiber biosensor comprises light source, optically coupled system, fibre-optical probe, rear beam splitting system, sample load carrier and six part compositions of control system.
Embodiment 1:
Below in conjunction with accompanying drawing, the present invention will be further described:
As shown in Figure 1, the present invention be non-marked light reflection interference optical fiber biosensor by light source assembly 100, optically coupled system 200, fibre-optical probe 300, rear beam-splitting optical system 400, embedded control system 500,
Sample load carrier 600, man-machine interactive system 700 and power supply 800 be totally 8 part compositions.
Wherein each optical module 100,200,300 and 400, is connected by standard bindiny mechanism.Entirety is without motion.The all band light that light source assembly 100 sends, enter fibre-optical probe 300 through incident optical 202, reflect at optical fiber 301 and 303 two end faces of chemical reagent decorative layer respectively, two bundle reflected light are after interfering, former road is returned, be divided into two at y-type optical fiber Nodes, wherein a part of interference light is through outgoing optical fiber 202, enter rear beam-splitting optical system 400, through grating 403 light splitting, each wave band interference light intensity is received by array CCD 405, light signal changes electric signal into, electric signal is processed through embedded control system 500, by the man-machine interactive system 700 output optical fibres 300 distal end face thickness information of popping one's head in.
Fig. 2 is light source coupling optical path figure, wherein, the 101st, light source, the 102nd, convex lens, the 201st, optical fiber A, cardinal principle is to utilize the focusing function of convex lens 102, the light that light source 101 is sent focuses on fibre core end face, improves light source utilization ratio, reduces direct-coupled assembly difficulty simultaneously.
Fig. 3 is system light path schematic diagram, wherein, the 200th, optically coupled system, 201 incidents are optical fiber, the 202nd, outgoing optical fiber, the 203rd, conduction optical fiber, wherein in optically coupled system 200, optical fiber used is multimode optical fiber, utilize optical detection means, the end face of incident optical 201 and outgoing optical fiber 202 is accurately aimed at the end face of conduction optical fiber 203, ensure the efficient coupling between incident optical 201, outgoing optical fiber 202 and conduction optical fiber 203, between each optical module, be connected and realize efficiency light coupling with other equipment by standard web member.As shown, the light approach incident optical 201 that light source 100 sends is coupled into conduction optical fiber 203, there are two secondary reflections in two rete places at fibre-optical probe 300 far-ends, two bundle reflected light are coupled at conduction optical fiber 203, and be two at switching node place portion, wherein part beam-splitting optical system 400 after outgoing optical fiber 202 enters, in rear beam-splitting optical system, 400 places complete opto-electronic conversion, gained electric signal, through follow-up system processing, is outwards exported interference information.
Fig. 4 is optical fiber probe far-end schematic diagram, and wherein, 401 is the optical fiber D that length is shorter, and its near-end is coupled to y-type optical fiber, and far-end is the biomolecule action end of fibre-optical probe.Biomolecule action end comprises 402 high index of refraction interfering layers; 403 chemical reagent decorative layers, it has bio-compatibility; 404 bio-molecule layer.The 405th, the determinand combining with bio-molecule layer.High index of refraction interfering layer is the first reflecting surface, adopts refractive index to be prepared from higher than the material of fiber core refractive index; Bio-molecule layer is the second reflecting surface, be one deck can with the biomolecule of sample to be tested specific binding.A is incident light, and b is the first reflected light, and c is the second reflected light.
Optical fiber probe prepare the fibre core end face pre-treatment that emphasis is optical fiber far-end, main five steps of processing procedure:
(1) optical fiber processing: in probe, optical fiber hand cut used becomes 40mm long, adopts optical fiber lens finishing method by end face polishing.
(2) preparation of high index of refraction interfering layer: this layer is high-index material, is prepared into little molecule rete by ion assisted physical vapor deposition coating machine, and thickness is nm magnitude.
(3) SiO
2the preparation of layer: adopt ion beam sputtering technology to prepare SiO
2layer, thickness is less than 1000nm.
(4) chemical reagent decorative layer: method 1 directly has the high molecular polymer of bio-compatibility in the finishing of high index of refraction interfering layer, can combine by specific adsorption with biomolecule.Method 2 adopts ion beam sputtering technology to prepare SiO on high index of refraction interfering layer surface
2layer, adopts suitable silane coupling agent at SiO
2finishing polar group, biomolecule combines by covalent bond with polar group.
(5) sessile antibody layer: this layer is treated detectable antigens and had high specific, realizes binding by the mode such as specific adsorption, covalent bonds and chemical reagent decorative layer.This antibody layer is sequence number 304 in Fig. 4.
(6) immobilized antigen layer: antigen, by specific adsorption, combines with the specific antibody in sessile antibody layer.
This antibody layer is sequence number 305 in Fig. 4.
Fig. 5 is rear beam splitting system, compound interference light is beam splitting system after incident crack 401 enters into, after concave mirror 402 collimations, be irradiated to grating surface, polychromatic light is after grating 403 light splitting, and the light of each wavelength, through concave mirror 404 shaping couplings, is received by the pixel of line array CCD 405 correspondences, realize the conversion of light signal to electric signal, and outwards export the interference light intensity information of each wavelength.
Fig. 6 is embedded control system, is program control system.Wherein 701 is display screens, the 702nd, and thermal printer adopts external power adapter power supply; Each wave band interference light intensity information of being inputted by rear beam-splitting optical system 400 completes matching in this system, and according to obtained intensity/wavelength curve, parse the required testing result of test item, the display screen 701 of testing result in man-machine exchange system 700 shows, coherent detection report is exported by built-in thermal printer 702, and print result can retain for a long time; Rear beam-splitting optical system is the correlation parameter that need to arrange in the time detecting, and as integral time etc., inputs by display screen 701.
Claims (2)
1. a non-marked light reflection interference optical fiber biosensor, is characterized in that:
Adopt all band halogen tungsten lamp as light source, broadband is optically coupled into optical fiber by scioptics collimating apparatus;
" Y " type of employing fiber coupler, and the source optical fiber being connected with light source, the Transmission Fibers being connected with fibre-optical probe and the detection fiber being connected with rear beam splitting system;
Fibre-optical probe is made up of multimode optical fiber, three layers of far-end punishment: (1), high index of refraction interfering layer; (2), chemical reagent decorative layer, it has bio-compatibility; (3), bio-molecule layer; There are two secondary reflections by the high refractive index layer at end face and bio-molecule layer in incident light, the interference light reflecting to form for twice is beam splitting system after y-type optical fiber one end enters;
Rear beam splitting system is made up of entrance slit, concave mirror, grating and array receiver, the light that light source sends shines directly into fibre-optical probe surface after collimation, after being irradiated to after collimation converges, reflected light in beam-splitting optical system, realizes detection to measured signal by signal acquisition device.
2. a kind of non-marked light reflection interference optical fiber biosensor as claimed in claim 1, is characterized in that: fiber type is multimode optical fiber core, core diameter 400 μ m, and wave band is 200-1100nm, three ends are all standard bindiny mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410247857.3A CN103994978A (en) | 2014-06-06 | 2014-06-06 | Label-free light reflection interference optical fiber biosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410247857.3A CN103994978A (en) | 2014-06-06 | 2014-06-06 | Label-free light reflection interference optical fiber biosensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103994978A true CN103994978A (en) | 2014-08-20 |
Family
ID=51309202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410247857.3A Pending CN103994978A (en) | 2014-06-06 | 2014-06-06 | Label-free light reflection interference optical fiber biosensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103994978A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105891155A (en) * | 2016-04-08 | 2016-08-24 | 山东大学 | Label-free optical fiber biosensing probe based on Fabry-Perot interference |
| WO2024052771A1 (en) * | 2022-09-08 | 2024-03-14 | Sartorius Bioanalytical Instruments, Inc. | Biolayer interferometry sensor with an optical fiber having a faceted cross-section and apparatus for using the same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2662247Y (en) * | 2003-04-25 | 2004-12-08 | 谭玉山 | Optical fibre array biochip based on transformation rule of white light reflection interference frequency spectrum |
| CN101769857A (en) * | 2010-01-06 | 2010-07-07 | 哈尔滨工程大学 | Plasma resonant type optical fiber biosensor based on annular core wave guide |
| CN101776595A (en) * | 2010-01-29 | 2010-07-14 | 武汉理工大学 | Manufacture method of optical fiber humidity sensor based on Fabry-Perot interference |
| CN203908938U (en) * | 2014-06-06 | 2014-10-29 | 长春理工大学 | Non-marked light reflection interference optical fiber biosensor |
-
2014
- 2014-06-06 CN CN201410247857.3A patent/CN103994978A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2662247Y (en) * | 2003-04-25 | 2004-12-08 | 谭玉山 | Optical fibre array biochip based on transformation rule of white light reflection interference frequency spectrum |
| CN101769857A (en) * | 2010-01-06 | 2010-07-07 | 哈尔滨工程大学 | Plasma resonant type optical fiber biosensor based on annular core wave guide |
| CN101776595A (en) * | 2010-01-29 | 2010-07-14 | 武汉理工大学 | Manufacture method of optical fiber humidity sensor based on Fabry-Perot interference |
| CN203908938U (en) * | 2014-06-06 | 2014-10-29 | 长春理工大学 | Non-marked light reflection interference optical fiber biosensor |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105891155A (en) * | 2016-04-08 | 2016-08-24 | 山东大学 | Label-free optical fiber biosensing probe based on Fabry-Perot interference |
| CN105891155B (en) * | 2016-04-08 | 2019-05-07 | 山东大学 | A kind of label-free fiber-optic biosensor probe based on enamel Fabry-Parot interferent |
| WO2024052771A1 (en) * | 2022-09-08 | 2024-03-14 | Sartorius Bioanalytical Instruments, Inc. | Biolayer interferometry sensor with an optical fiber having a faceted cross-section and apparatus for using the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106596474B (en) | Three-channel SPR sensor based on seven-core optical fiber | |
| CN108680505B (en) | Multichannel wavelength modulation type optical fiber SPR detection system | |
| JP3816072B2 (en) | Optical waveguide sensor and measuring device using the same | |
| CN106990086B (en) | Multi-channel evanescent wave all-fiber biosensor | |
| JP3152758U (en) | Surface plasma resonance measuring instrument | |
| CN103245641B (en) | Multi-channel planar waveguide evanescent wave biosensor | |
| CN110243795B (en) | Evanescent wave bicolor fluorescent all-fiber biosensor | |
| CN105973807A (en) | Optical fiber sensing probe for detecting gas and liquid | |
| CN101199413A (en) | Optical coherence tomography method and equipment therefor | |
| CN104155266A (en) | Multichannel parallel detection surface plasma resonance biosensor, and preparation and detection methods thereof | |
| US20230194425A1 (en) | Optical sensor of bio-molecules using interferometer | |
| CN211235540U (en) | Optical fiber long-range surface plasma resonance biosensor and sensing system | |
| CN103940796A (en) | Novel multi-angle and multi-mode quick switching circular optical illumination microscopic imaging system | |
| CN102095719A (en) | Optical fiber type sensor system based on surface plasma resonance and stimulated Raman scattering | |
| Peng et al. | Compact surface plasmon resonance imaging sensing system based on general optoelectronic components | |
| CN104458587A (en) | Multichannel unmarked biosensing optical fiber system | |
| CN208350613U (en) | A kind of multi-channel wavelength modulation type optical fiber detection system | |
| CN203908938U (en) | Non-marked light reflection interference optical fiber biosensor | |
| US7831126B2 (en) | Waveguide core and biosensor | |
| CN103994978A (en) | Label-free light reflection interference optical fiber biosensor | |
| CN104215607A (en) | Optical fiber cantilever beam sensor for food pathogenic bacteria and detection method | |
| CN1712929A (en) | MZ interference evanescent wave chemical and biological sensor and system with fibre-optical microstructure | |
| CN100567957C (en) | A kind of many optical fiber surface plasmon resonance probes with temperature correction | |
| CN101520410A (en) | Unmarked combined few-mode fiber evanescent field biosensor | |
| CN102636462B (en) | On-line purified multimode conduction surface plasma resonance spectrometer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140820 |