CN211235540U - Optical fiber long-range surface plasma resonance biosensor and sensing system - Google Patents
Optical fiber long-range surface plasma resonance biosensor and sensing system Download PDFInfo
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- CN211235540U CN211235540U CN201922122474.4U CN201922122474U CN211235540U CN 211235540 U CN211235540 U CN 211235540U CN 201922122474 U CN201922122474 U CN 201922122474U CN 211235540 U CN211235540 U CN 211235540U
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Abstract
The utility model provides an optic fibre long distance surface plasmon resonance biosensor and sensing system, the sensor is including getting rid of the side of coating and throwing the big core footpath optic fibre of grinding, has plated the matching layer on the throwing mill face of big core footpath optic fibre side throwing grinding, matching layer surface metallization membrane, the metal film passes through coupling agent fixed antibody, the antibody combines with the antigen. The utility model provides an optic fibre long distance surface plasmon resonance biosensor that dopamine was modified is based on long distance surface plasmon resonance, compares in conventional surface plasmon resonance, and the penetration depth of long distance surface plasmon in the determinand is darker, and the propagation distance between metal level and determinand interface is longer. Therefore, the sensitivity and the detection precision of the sensor are higher.
Description
Technical Field
The utility model relates to a biological solution of low concentration detects technical field, in particular to optic fibre long-range surface plasmon resonance biosensor and sensing system.
Background
In the existing detection technology for low-concentration biological solutions such as nucleic acid, protein and the like, the electrochemical method has low sensitivity and poor stability; the enzyme linked immunosorbent assay has high requirements on the activity of the enzyme and the performance of instruments, so the cost is high, and the change of the external environment temperature probably influences the activity of the enzyme; the fluorescence method can affect the activity of the detected object, and the detection time is longer; the commercialized surface plasmon resonance instrument has high sensitivity, good stability and capability of realizing rapid detection, but is expensive, large in volume and not suitable for remote measurement. Therefore, the optical fiber surface plasmon resonance sensor has attracted more and more attention due to its advantages of high sensitivity, simple manufacture, small volume, anti-electromagnetic interference, easy multiplexing to form distributed sensing, and suitability for remote measurement.
The detection method for a typical low concentration biological solution is antigen-antibody immunobinding, and the concentration of the biological solution used for detection analysis is generally in the order of μ g/mL. However, with the rapid development of biomass detection technology, the sensitivity and detection accuracy level that can be achieved by the conventional optical fiber surface plasmon resonance sensor at present cannot well meet the detection requirements, but the sensor is required to have better sensing performance.
SUMMERY OF THE UTILITY MODEL
The technical task of the utility model is not enough to above prior art, provides an optic fibre long-range surface plasmon resonance biosensor and sensing system, has solved among the prior art optic fibre surface plasmon resonance sensor sensitivity not high, half peak width broad, detects the technical problem that the precision is low, has realized the measurement to the high sensitivity and the high detection precision of low concentration biological solution.
The utility model provides a technical scheme that its technical problem adopted is:
an optical fiber long-range surface plasmon resonance biosensor comprises a large-core-diameter optical fiber, wherein a coating layer is removed, the side edge of the large-core-diameter optical fiber is polished, a polishing surface of the large-core-diameter optical fiber, which is polished, is plated with a matching layer, the surface of the matching layer is plated with a metal film, the metal film fixes an antibody through a coupling agent, and the antibody is combined with an antigen.
Furthermore, the matching layer is a Tb (III) fluorine-containing organic complex film which is compact and flat, has the thickness of 100-200 nm and the refractive index of 1.35.
Furthermore, the metal film is a compact and flat gold film with the thickness of 30-40 nm.
Furthermore, the coupling agent is a compact and flat dopamine film with the thickness of 10-20 nm.
Furthermore, the polishing depth of the side edge of the large-core optical fiber is 52-54 mu m, the length of a polishing area is 10-12 mm, and the total length of the optical fiber is 5-10 cm.
Further, the large-core optical fiber has a core refractive index of 1.4678, a cladding refractive index of 1.4612, a core diameter of 105 μm, and a cladding diameter of 125 μm.
An optical fiber long-range surface plasma resonance biological sensing system comprises a wide-spectrum light source, a multimode optical fiber, a spectrometer and an optical fiber sensing unit based on the sensor, wherein the input end of the optical fiber sensing unit is connected with the wide-spectrum light source through the multimode optical fiber, and the output end of the optical fiber sensing unit is connected with the spectrometer through the multimode optical fiber.
Furthermore, the spectrometer is a spectrometer with a detection wavelength range of 250 nm-1180 nm and an optical resolution of 0.82 nm.
Furthermore, the core refractive index of the multimode fiber is 1.468, the cladding refractive index is 1.461, the core diameter is 62.5 μm, the cladding diameter is 125 μm, and the length is 200 mm.
Furthermore, the wide-spectrum light source is a continuous-spectrum deuterium halogen lamp light source without mutation, and the wavelength of the light source continuously changes within the range of 400nm to 1000 nm.
Compared with the prior art, the utility model beneficial effect be:
the utility model uses dopamine as antibody coupling agent, and the good biocompatibility and strong hydrolysis resistance of the dopamine greatly improve the fixation efficiency of the antibody, thereby improving the detection performance of the sensor; the full width of the resonance valley half maximum of the sensor is reduced by utilizing the deep penetration depth of the long-range surface plasma in the object to be measured and the long propagation distance between the object to be measured and the interface of the metal film, the detection precision and the sensitivity of the sensor are improved, the loss of plasma waves in the sensor is greatly reduced by adding the matching layer, and the sensing has higher sensitivity and precision. The utility model discloses compare and have higher detection precision and sensitivity in ordinary optic fibre surface plasma resonance sensor, and can realize remote dynamic real-time supervision, its compact structure can wide application in fields such as chemistry, biology, medical treatment, food safety.
Drawings
Fig. 1 is a schematic structural diagram of a sensor according to the present invention;
fig. 2 is a schematic structural diagram of the sensing system of the present invention;
FIG. 3 is a structural formula of Tb (III) fluorine-containing organic complex;
FIG. 4 shows the transmission spectra obtained by the sensor of the present invention when detecting antigen solutions of different concentrations;
FIG. 5 is a dynamic graph showing the shift amount of the resonance wavelength corresponding to each concentration of the antigen solution and the concentration of the corresponding antigen;
reference numerals:
1-a wide spectrum light source, 2-a multimode optical fiber, 3-an optical fiber sensing unit and 4-a spectrometer;
31-side polishing of a large-core optical fiber, 32-matching layer, 33-metal film, 34-dopamine film, 35-antibody and 36-antigen.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.
Examples
As shown in fig. 1, the optical fiber long-range surface plasmon resonance biosensor of the present invention comprises a large core diameter optical fiber 31 polished by a side edge without a coating layer, a matching layer 32 plated on a polished surface polished by the side edge of the large core diameter optical fiber, a metal film 33 plated on the surface of the matching layer, an antibody 35 fixed on the metal film through a coupling agent 34, and the antibody combined with an antigen 36; the matching layer is a Tb (III) fluorine-containing organic complex film which is compact and flat, has the thickness of 100-200 nm and the refractive index of 1.35; the metal film is a compact and flat gold film with the thickness of 30-40 nm; the coupling agent is a compact and flat dopamine film with the thickness of 10-20 nm.
As shown in FIG. 2, optic fibre long distance surface plasma resonance biological sensing system, including wide spectrum light source 1, multimode optic fibre 2, based on the optic fibre sensing unit 3 of above-mentioned sensor, spectrum appearance 4, the wide spectrum light source is connected through multimode optic fibre to optic fibre sensing unit input, and the output passes through multimode optic fibre and is connected with the spectrum appearance.
The refractive index of a fiber core of the multimode fiber is 1.468, the refractive index of a cladding is 1.461, the diameter of the fiber core is 62.5 mu m, the diameter of the cladding is 125 mu m, the length of the multimode fiber is 200mm, and the wide-spectrum light source is a continuous-spectrum deuterium halide lamp light source without mutation, wherein the wavelength of the continuous-spectrum deuterium halide lamp light source continuously changes within the range of 400 nm-1000 nm.
The preparation method of the optical fiber long-range surface plasmon resonance biosensor comprises the following steps:
(1) preparation of side-polished large-core optical fiber
Removing the large-core-diameter optical fiber with the coating layer, wherein the fiber core refractive index is 1.4678, the cladding refractive index is 1.4612, the fiber core diameter is 105 micrometers, the cladding diameter is 125 micrometers, the total length of the optical fiber is 10cm, the large-core-diameter optical fiber is polished on the side edge, the rotation and the propulsion of a polishing grinding wheel are controlled by a motor and a computer, the cladding on one side of the optical fiber is gradually polished, the polishing depth of the optical fiber is observed in real time by a microscope, the length of a polishing area is 10mm, and the polishing depth is 53 micrometers;
when the side edge of the large-core optical fiber is polished, two ends of the optical fiber are respectively connected with the light source and the spectrometer, so that the loss condition of the optical power in the output spectrum can be monitored in real time, and the polishing can be stopped when the required polishing depth is obtained through microscope observation;
(2) plating matching layer
Placing the side-polished large-core optical fiber in a pulling and coating machine, coating a matching layer 32 on the surface of a polishing and polishing area of the optical fiber, setting the pulling speed of the pulling and coating machine to be 20mm/min, wherein the matching layer is a compact and flat Tb (III) fluorine-containing organic complex film with the thickness of 100nm and the refractive index of 1.35, and the structural formula of the Tb (III) fluorine-containing organic complex is shown in figure 3;
(3) gold plating film
Placing the optical fiber in a vacuum ion beam sputtering instrument, and sputtering a gold film 33 on the surface of the optical fiber, wherein the current of the vacuum ion beam sputtering instrument is 7mA, the time is 2 minutes, and the thickness of the gold film is 30 nm;
(4) modified dopamine
And (3) placing the optical fiber plated with the gold film in a dopamine solution of 2mg/mL for soaking for 30min, and growing a dopamine film with the thickness of 12nm on the surface of the gold film through the self-polymerization effect of dopamine.
(4) Immobilized antibodies
The antibody is mouse anti-human immunoglobulin, the optical fiber modified with dopamine is placed in 100 mug/mL mouse anti-human immunoglobulin solution for overnight incubation, and a layer of antibody is fixed on the surface of the optical fiber.
(5) Detection of antigens
The antigen is human immunoglobulin solution with the concentration of 1 mug/mL, 2 mug/mL, 5 mug/mL, 10 mug/mL, 15 mug/mL, 20 mug/mL, 25 mug/mL, 30 mug/mL, 35 mug/mL and 40 mug/mL in sequence, the optical fiber fixed with the antibody is placed in the human immunoglobulin solution with different concentrations, the molecular mass of the sensor surface is changed along with the continuous combination of the antigen and the antibody, further, the condition of surface plasma resonance is changed, and the resonance valley in the transmission spectrum of the sensing unit is moved. The resonance wavelength shift amount corresponding to each concentration of antigen solution was fitted to the corresponding antigen concentration, and the tangential slope of the curve at a concentration of 1. mu.g/mL was determined as the sensor sensitivity SnCalculated Sn0.96nm/(μ g/mL), and the spectrometer resolution Δ λ is 0.12nm divided by the sensor sensitivity SnThe sensor detection limit LOD can be obtained, and the specific result can be expressed as:
LOD＝Δλ/Sn
the utility model discloses the sensor of above-mentioned preparation is detecting the transmission spectrum that different concentration antigen solution obtained as shown in figure 4, and the resonance wavelength shift amount that the antigen solution of each concentration corresponds and the dynamic curve that corresponds antigen concentration are shown in figure 5, and the inset is the curve tangent slope of curve when first concentration.
The LOD was 0.125 μ g/mL by substituting the above data. From this the utility model discloses a detection to low concentration biological solution high sensitivity and low detection limit. In addition, each resonance valley in the sensor transmission spectrum has a narrow full width at half maximum, so that the sensor detection accuracy is high.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.
Claims (10)
1. An optical fiber long-range surface plasmon resonance biosensor is characterized in that: the method comprises the steps of removing a side edge polishing large-core-diameter optical fiber of a coating layer, plating a matching layer on a polishing surface of the side edge polishing of the large-core-diameter optical fiber, plating a metal film on the surface of the matching layer, fixing an antibody through a coupling agent by the metal film, and combining the antibody with an antigen.
2. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the matching layer is a Tb (III) fluorine-containing organic complex film with the thickness of 100-200 nm and the refractive index of 1.35.
3. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the metal film is a gold film with the thickness of 30-40 nm.
4. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the coupling agent is a dopamine film with the thickness of 10-20 nm.
5. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the matching layer is a Tb (III) fluorine-containing organic complex film with the thickness of 100nm and the refractive index of 1.35; the thickness of the metal film is 30 nm; the coupling agent is a dopamine film with the thickness of 12 nm.
6. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the large-core optical fiber has a core refractive index of 1.4678, a cladding refractive index of 1.4612, a core diameter of 105 μm and a cladding diameter of 125 μm.
7. The optical fiber long-range surface plasmon resonance biosensor according to claim 1, wherein: the polishing depth of the side edge of the large-core-diameter optical fiber is 52-54 mu m, the length of a polishing area is 10-12 mm, and the total length of the optical fiber is 5-10 cm.
8. An optical fiber long-range surface plasma resonance biosensing system is characterized in that: the sensor comprises a wide-spectrum light source, a multimode optical fiber, a spectrometer and an optical fiber sensing unit based on the sensor of claim 1, wherein the input end of the optical fiber sensing unit is connected with the wide-spectrum light source through the multimode optical fiber, and the output end of the optical fiber sensing unit is connected with the spectrometer through the multimode optical fiber.
9. The optical fiber long-range surface plasmon resonance biosensing system of claim 8, wherein: the spectrometer is used for detecting the wavelength range of 250 nm-1180 nm and the optical resolution of 0.82 nm.
10. The optical fiber long-range surface plasmon resonance biosensing system of claim 8, wherein: the wide-spectrum light source is a continuous-spectrum deuterium halide lamp light source without mutation, and the wavelength of the light source continuously changes within the range of 400nm to 1000 nm.
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Cited By (4)
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| CN111947697A (en) * | 2020-08-24 | 2020-11-17 | 重庆理工大学 | Novel fiber Bragg grating hydrogen sensor and manufacturing method |
| CN112461794A (en) * | 2020-11-13 | 2021-03-09 | 天津大学 | Long-range SPR sensor and preparation method thereof |
| CN112730339A (en) * | 2020-12-24 | 2021-04-30 | 东北大学 | Based on Ti3C2High-sensitivity LRSPR optical fiber sensor and manufacturing method thereof |
| CN112834463A (en) * | 2020-12-31 | 2021-05-25 | 桂林电子科技大学 | Double-side polished plastic optical fiber enhanced SPR sensor and preparation method thereof |
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2019
- 2019-12-02 CN CN201922122474.4U patent/CN211235540U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111947697A (en) * | 2020-08-24 | 2020-11-17 | 重庆理工大学 | Novel fiber Bragg grating hydrogen sensor and manufacturing method |
| CN112461794A (en) * | 2020-11-13 | 2021-03-09 | 天津大学 | Long-range SPR sensor and preparation method thereof |
| CN112461794B (en) * | 2020-11-13 | 2022-09-16 | 天津大学 | Long-range SPR sensor and preparation method thereof |
| CN112730339A (en) * | 2020-12-24 | 2021-04-30 | 东北大学 | Based on Ti3C2High-sensitivity LRSPR optical fiber sensor and manufacturing method thereof |
| CN112730339B (en) * | 2020-12-24 | 2021-12-14 | 东北大学 | Based on Ti3C2High-sensitivity LRSPR optical fiber sensor and manufacturing method thereof |
| CN112834463A (en) * | 2020-12-31 | 2021-05-25 | 桂林电子科技大学 | Double-side polished plastic optical fiber enhanced SPR sensor and preparation method thereof |
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