CN205590709U - Bionical enzymatic hydrolysis device - Google Patents

Abstract

The utility model discloses a bionic enzymolysis device, which comprises a tank body with an interlayer, an interface through the interlayer on the upper part of the tank body, and a three-way valve on the interface, which is respectively connected to a steam pipe and a water outlet pipe. There are steam valves and water stop valves respectively on the steam pipe and the water outlet pipe; in the middle of the tank body, there are glass observation windows, lighting systems, and temperature measuring devices through the tank body interlayer; The lower part has a pH value measuring device and a temperature measuring device inside the tank; there is a stirring device at the top of the tank, and the stirring end of the stirring device extends into the tank; there is an acid adding device at the top of the tank. Liquid port, lye adding port, enzyme liquid port, water adding port, exhaust port, and feeding port, each of which has a An isolation valve; there is a discharge port at the bottom of the tank body, and an isolation valve is provided at the connection between the discharge port and the tank body; there is an interface through the interlayer at the bottom end of the tank body, and the interface has a three-way valve, The water inlet pipe and the condensed water drain pipe are connected respectively, and the water inlet pipe and the condensed water drain pipe are respectively provided with water stop valves. The device can adapt to the bionic enzymolysis method to extract bioactive peptide components, and can complete the bionic enzymolysis extraction of materials exclusively, faster and more accurately.

Description

A biomimetic enzymolysis device

technical field

The invention relates to the field of biomedical equipment, in particular to a bionic enzymolysis device.

Background technique

In most pharmaceutical companies, the enzymatic hydrolysis process adopts enzymatic hydrolysis tanks with stirring paddles and insulation interlayers provided by unified pharmaceutical equipment factories, and artificially controls different enzyme types and enzymatic hydrolysis processes. Biomimetic enzymatic hydrolysis technology is an enzymatic hydrolysis method created by us according to the digestion and absorption process of human body after taking protein. It is a continuous double-enzyme hydrolysis of pepsin and trypsin under certain conditions. The manipulation of tank enzymatic hydrolysis is not strong, and it is not easy to achieve stable control. Therefore, according to the technical characteristics of biomimetic enzymolysis, we designed a special enzymolysis device for biomimetic enzymolysis technology.

Contents of the invention

The purpose of the utility model is to provide a special enzymolysis device for bionic enzymolysis technology.

To achieve the above object, the technical solution of the utility model is:

A biomimetic enzymolysis device, comprising a tank body with an interlayer, an interface through the interlayer on the upper part of the tank body, the interface has a three-way valve, and is respectively connected to a steam pipe and a water outlet pipe, and the steam pipe and the water outlet pipe There are steam valves and water stop valves on the top; in the middle of the tank, there are glass observation windows, lighting systems, and temperature measuring devices for the interlayer of the tank; in the lower part of the tank, there are tanks Internal pH value measuring device and temperature measuring device; there is a stirring device at the top of the tank body, and the stirring end of the stirring device extends into the tank body; One liquid port, one enzyme liquid port, one water feed port, one exhaust port, and one feed port, and each of the acid liquid feed port, alkali liquid feed port, enzyme liquid feed port, water feed port, and exhaust port is equipped with an isolation valve; There is a discharge port at the bottom of the tank body, and there is an isolation valve at the connection between the discharge port and the tank body; there is an interface through the interlayer at the bottom end of the tank body, and the interface has a three-way valve, respectively connected to the water inlet pipe and the condensed water drain pipe, the water inlet pipe and the condensed water drain pipe are respectively provided with water stop valves.

The device can adapt to the bionic enzymolysis method to extract bioactive peptide components, and can complete the bionic enzymolysis extraction of materials exclusively, faster and more accurately.

The technical effect of the utility model is: the device is a special enzymolysis device for biomimetic enzymolysis technology, which is highly targeted, can control the technical characteristics of biomimetic enzymolysis, and can complete the biomimetic biomimesis of materials faster and more accurately. Enzymolysis extraction; the device pre-calculates the amount of various materials added before enzymolysis, and puts them in the storage hopper, and adds them on time during the enzymolysis process. The operation is simple and the labor intensity is small; the device can dynamically detect the enzymolysis on-line The pH value and temperature of the enzymatic hydrolysis solution in the process can be supplemented with acid or alkali solution at any time, and the temperature can be compensated to make the enzymatic hydrolysis process highly controllable.

Description of drawings

Figure 1. Schematic diagram of the biomimetic enzymatic hydrolysis device.

detailed description

Below in conjunction with accompanying drawing 1 further illustrate the specific embodiment of the present utility model.

Referring to Figure 1, calculate the amount of enzymatic hydrolysis materials and the amount of pepsin and trypsin required, prepare pepsin into an aqueous suspension, and put it in an enzyme material tank; prepare acid and lye, and place them separately In the acid material tank and the alkali material tank; add water from the water inlet to the enzymolysis tank to the required amount; start the stirring paddle; add medicinal material powder from the feeding port, close the feeding port; open the steam valve, feed steam from the steam pipe, and detect The temperature in the tank is raised to 80°C, start timing, and after 15 minutes, close the steam valve; open the water valve, pass in cooling water from the water inlet pipe, and discharge water from the outlet pipe, check the temperature in the tank to 40°C, close the water valve; start the interlayer electric Heating, when the interlayer temperature measurement device measures the temperature to 40°C, start the heat preservation; open the isolation valve, add acid solution from the acid solution port, and the pH measuring device detects the pH value, and close the isolation valve when it reaches 2.0; open the isolation valve, add the acid solution from the enzyme solution Add pepsin suspension to the port, close the isolation valve; time the enzymolysis for 1 hour, monitor the temperature and pH value at any time, and open the isolation valve every time the pH value exceeds 2.2, add acid solution from the acid solution port, and the pH measuring device detects the pH value , close the isolation valve when it reaches 2.0, and at the same time, prepare trypsin into an aqueous suspension and add it to the enzyme feeder; open the isolation valve, add lye from the lye adding port, and the pH measuring device detects the pH value, and close the isolation valve when it reaches 8.0 ;Open the isolation valve, add trypsin suspension from the enzyme liquid port, close the isolation valve; time the enzymolysis for 3 hours, monitor the temperature and pH value at any time, and open the isolation valve every time the pH value is lower than 7.8. Add lye, the pH measuring device detects the pH value, and closes the isolation valve when it reaches 8.0; opens the isolation valve, adds acid solution from the acid solution port, and the pH measuring device detects the pH value, and closes the isolation valve when it reaches 7.0; Drain the interlayer water from the drain pipe; open the steam valve, feed steam from the steam pipe, heat up the temperature in the detection tank to 80°C, start timing, and close the steam valve after 15 minutes; open the isolation valve, discharge the enzymatic solution from the discharge port to high speed Centrifuge in a centrifuge; the supernatant proceeds to the next step of ultrafiltration, nanofiltration (or electrodialysis), and column system purification.

This patent is not limited to the above embodiments. The content not described in detail in this specification belongs to the prior art known to those skilled in the art.

Claims (1)

1. A biomimetic enzymolysis device, comprising a tank body with an interlayer, on the upper part of the tank body there is an interface through the interlayer, and the interface has a three-way valve, which is respectively connected to the steam pipe (1) and the water outlet pipe (2) , there are steam valves (a) and water stop valves (b) on the steam pipe (1) and water outlet pipe (2) respectively; in the middle of the tank body there is a glass observation window (11) through the inside of the tank body, lighting System (10), a temperature measuring device (12) through the interlayer of the tank body; at the lower part of the tank body, there are a pH value measuring device (13) and a temperature measuring device (14) inside the tank body; There is a stirring device (9) on the top, and the stirring end of the stirring device (9) extends into the tank; on the top of the tank, there are acid liquid inlet (3), alkali liquid inlet (4), and Enzyme liquid port (5), water inlet (6), exhaust port (7), feed port (8), one for acid liquid port (3), lye liquid port (4), enzyme liquid port (5), the water inlet (6), and the exhaust port (7) each have an isolation valve (c), (d), (e), (f), (g); at the bottom of the tank there is a Outlet (17), there is an isolation valve (j) at the connection between the outlet (17) and the tank; at the bottom of the tank, there is an interface through the interlayer, and the interface has a three-way valve, respectively connected to the inlet The water pipe (15) and the condensed water drain pipe (16), the water inlet pipe (15) and the condensed water drain pipe (16) are respectively provided with water stop valves (h) and (i).