CN205590709U

CN205590709U - Bionical enzymatic hydrolysis device

Info

Publication number: CN205590709U
Application number: CN201620048585.9U
Authority: CN
Inventors: 代龙; 刘国飞
Original assignee: Anhui Biological Peptide Industry Research Institute
Current assignee: Anhui Biological Peptide Industry Research Institute
Priority date: 2016-01-19
Filing date: 2016-01-19
Publication date: 2016-09-21
Anticipated expiration: 2026-01-19

Abstract

The utility model discloses a bionical enzymatic hydrolysis device, including a jar body, the jar is personally experienced sth. Part of the body and is taken the intermediate layer there is the interbedded interface of expert on the upper portion of the jar body, and the interface has the three -way valve, inserts steam pipe and outlet pipe respectively, there are steam valve and stagnant water valve on steam pipe and the outlet pipe respectively, there is a glass observation window, lighting system, the logical jar of interbedded temperature measuring device of the body who leads to the internal portion of jar at the middle part of the jar body, the lower part of the jar body has a pH value of leading to the internal portion of jar to survey a device, temperature measuring device, there is agitating unit on jar body top, agitating unit's stirring end stretches into the jar is internal, the top of jar body have with the acidizing fluid mouth, add alkali lye mouth, enzyme -added liquid mouth, filler, gas vent, charge door each one, add the acidizing fluid mouth, respectively take an isolating valve with alkali lye mouth, enzyme -added liquid mouth, filler, gas vent, there is a discharge gate bottom of the jar body, an isolating valve is located to have with tank connection by the discharge gate, there is the interbedded interface of expert the bottom of the jar body, and the interface has the three -way valve, inserts inlet tube and comdenstion water drain pipe respectively, there is the stagnant water valve on inlet tube and the comdenstion water drain pipe respectively. Biological activity peptide class composition is drawed to method that the device can adapt to bionical enzymolysis, can be exclusive, sooner, the bionical enzymolysis of more accurate completion material draws.

Description

A kind of bionic enzymatic device

Technical field

The present invention relates to biological medicine apparatus field, be related specifically to a kind of bionic enzymatic device.

Background technology

In most of pharmaceutical manufacturers, enzymolysis process is all band stirring paddle, the enzymatic vessel of heat preservation sandwich layer using unified medical equipment factory to provide, and for different enzyme classes, enzymolysis process, carries out Artificial Control.Bionic enzymatic technology is the process digested and assimilated after we take protide according to human body and the enzyme solution founded, for pepsin, trypsin the most double enzyme enzymolysis under certain condition, this enzymolysis process is handling the strongest with common enzymatic vessel enzymolysis, it is not easy to reach stability contorting.Still for the technical characterstic of bionic enzymatic, design the enzymolysis device of bionic enzymatic technology-specific.

Summary of the invention

The purpose of this utility model is to provide the enzymolysis device of a kind of bionic enzymatic technology-specific.

For achieving the above object, the technical solution of the utility model is:

A kind of bionic enzymatic device, including tank body, tank body sandwich, has the interface of logical interlayer on the top of described tank body, and interface has three-way valve, is respectively connected to steam pipe and outlet pipe, and described steam pipe and outlet pipe have steam valve and water check valve respectively；The glass window of logical tank interior, illuminator, the temperature measuring equipment of logical tank body interlayer is had at the middle part of described tank body；The pH value determinator of logical tank interior, temperature measuring equipment is had in the bottom of described tank body；Having agitating device on described tank body top, the stirring end of described agitating device stretches in described tank body；Have acid liquid mouth on the top of described tank body, add alkali liquor mouth, enzyme-added liquid mouth, filler, air vent, charge door each, described acid liquid mouth, add alkali liquor mouth, enzyme-added liquid mouth, filler, air vent respectively carry an isolating valve；There are a discharging opening, described discharging opening and tank body junction to have an isolating valve in the bottom of described tank body；Have the interface of logical interlayer in the bottom of described tank body, interface has three-way valve, is respectively connected to water inlet pipe and condensate draining pipe, and described water inlet pipe and condensate draining pipe have water check valve respectively.

This device adapts to the method for bionic enzymatic and extracts biological activity peptide constituents, can extract by bionic enzymatic exclusive, that complete material faster, more accurately.

The special enzymolysis device having the technical effect that this device is bionic enzymatic technology of the present utility model, with strong points, it is possible to control the technical characterstic of bionic enzymatic targetedly, the bionic enzymatic that can complete material faster, more accurately extracts；This device is the addition of precalculated various materials before enzymolysis, is placed in storage hopper, adds in enzymolysis process on time, and simple to operate, labor intensity is little；This device can the pH value of dynamic on-line monitoring enzymolysis process enzymolysis solution, temperature, supplemental acid or alkali liquor, compensation temperature, make enzymolysis process controllability strong at any time.

Accompanying drawing explanation

Fig. 1. bionic enzymatic apparatus structure schematic diagram.

Detailed description of the invention

1 further illustrate detailed description of the invention of the present utility model below in conjunction with the accompanying drawings.

See Fig. 1, calculate amount and required pepsin, the tryptic amount of enzymolysis material, pepsin is configured to aqueous suspension, puts in enzyme batch can；Configure acid solution and alkali liquor, put respectively in acid batch can and alkali batch can；Add water to from filler in enzymatic vessel, to required amount；Start stirring slurry；Add medical material powder from charge door, close charge door；Open steam valve, be passed through steam from steam pipe, be warming up to 80 DEG C in detection tank, start timing, after 15 minutes, steam off valve；Opening water valve, be passed through cooling water from water inlet pipe, in outlet pipe water outlet, detection tank, temperature is to 40 DEG C, closes water valve；Starting interlayer electrical heating, interlayer temperature measuring equipment measures temperature and reaches 40 DEG C, starts insulation；Opening isolating valve, add acid solution from acid liquid mouth, pH determinator detection pH value, to closing isolating valve when 2.0；Open isolating valve, add pepsin suspension from enzyme-added liquid mouth, close isolating valve；Timing enzymolysis 1 hour, monitors temperature, pH value at any time, and pH value, often more than 2.2, is opened isolating valve, added acid solution from acid liquid mouth, and trypsin, to closing isolating valve when 2.0, is configured to aqueous suspension simultaneously and adds in enzyme glassware by pH determinator detection pH value；Open isolating valve, from add alkali liquor mouth add alkali liquor, pH determinator detection pH value, to when 8.0 close isolating valve；Open isolating valve, add trypsin suspension from enzyme-added liquid mouth, close isolating valve；Timing enzymolysis 3 hours, monitors temperature, pH value at any time, and pH value, often less than 7.8, is opened isolating valve, added alkali liquor from adding alkali liquor mouth, and pH determinator detection pH value, to closing isolating valve when 8.0；Opening isolating valve, add acid solution from acid liquid mouth, pH determinator detection pH value, to closing isolating valve when 7.0；Open water valve, discharge intermediate water from drain pipe；Open steam valve, be passed through steam from steam pipe, be warming up to 80 DEG C in detection tank, start timing, after 15 minutes, steam off valve；Open isolating valve, discharge enzymolysis solution from discharging opening to centrifugal high speed centrifuge；Clear liquid continues next step ultrafiltration, nanofiltration (or electrodialysis), column system purification.

This patent is not limited to above-mentioned embodiment.The content that this specification is not described in detail belongs to prior art known to professional and technical personnel in the field.

Claims

1. a bionic enzymatic device, including tank body, tank body sandwich, the interface of logical interlayer is had on the top of described tank body, interface has three-way valve, is respectively connected to steam pipe (1) and outlet pipe (2), has steam valve (a) and water check valve (b) on steam pipe (1) and outlet pipe (2) respectively；The glass window (11) of logical tank interior, illuminator (10), the temperature measuring equipment (12) of logical tank body interlayer is had at the middle part of described tank body；The pH value determinator (13) of logical tank interior, temperature measuring equipment (14) is had in the bottom of described tank body；There are agitating device (9), the stirring end of described agitating device (9) to stretch in described tank body on described tank body top；There is acid liquid mouth (3) on the top of described tank body, add alkali liquor mouth (4), enzyme-added liquid mouth (5), filler (6), air vent (7), charge door (8) each, described acid liquid mouth (3), add alkali liquor mouth (4), enzyme-added liquid mouth (5), filler (6), air vent (7) respectively band one isolating valve (c), (d), (e), (f), (g)；There are a discharging opening (17), described discharging opening (17) and tank body junction to have an isolating valve (j) in the bottom of described tank body；Have the interface of logical interlayer in the bottom of described tank body, interface has three-way valve, is respectively connected on water inlet pipe (15) and condensate draining pipe (16), described water inlet pipe (15) and condensate draining pipe (16) to have respectively water check valve (h), (i).