CN205538417U - Microorganism diluting device - Google Patents
Microorganism diluting device Download PDFInfo
- Publication number
- CN205538417U CN205538417U CN201620062454.6U CN201620062454U CN205538417U CN 205538417 U CN205538417 U CN 205538417U CN 201620062454 U CN201620062454 U CN 201620062454U CN 205538417 U CN205538417 U CN 205538417U
- Authority
- CN
- China
- Prior art keywords
- check valve
- syringe
- microorganism
- flexible pipe
- inlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a microorganism diluting device, includes the syringe, and the syringe lower extreme is equipped with inlet and liquid outlet, is connected with first hose and second hose on inlet and the liquid outlet respectively, and first check valve one end is connected with the straw with first hose connection, the other end, and second check valve one end is connected with the calandria with second hose connection, the other end, the installation opposite direction of first check valve installation direction and second check valve. The utility model provides a pair of microorganism diluting device through setting up double -barrelled syringe, utilizes the mixed and discharge dilution of aseptic formula suction of first check valve and second check valve completion liquid, has reduced the use amount of aseptic test tube during the microorganism dilution is operated, has saved 23 operating time.
Description
Technical field
This utility model relates to a kind of air mix facilities, and especially a kind of microorganism air mix facilities, it is dilute that it is applicable to microorganism
Release operation, belong to microbiological analysis detection technique field.
Background technology
In industries such as food, medicine, health, generally require and the microorganism in sample is diluted operation, routine operation
Process is: 1), takes sterile petri dish 9 and overlaps, indicates 10 with mark respectively-4、10-5、10-6(dilution factor) each 3 sets.Separately take 6 to fill
The test tube of 9ml sterilized water, indicates 10 successively-1、10-2、10-3、10-4、10-5、10-6.2), draw 1ml with 1ml aseptic straw to cultivate
Liquid joins and indicates 10-1In the test tube of printed words, this is 10 times of dilutions.3), test tube or is put vibration on agitator in palm, make
Bacterium solution fully mixes.Separately take a 1ml aseptic straw and insert 10-1The test tube of printed words is drawn 1ml in 10-2In test tube, this is
100 times of dilutions.4), test tube or is put vibration on agitator in palm, make bacterium solution fully mix.Separately take an aseptic suction of 1ml
Pipe inserts 10-2The test tube of printed words is drawn 1ml in 10-3In test tube, this is 1000 times of dilutions ... the rest may be inferred for remaining.5),
10 are drawn respectively with the aseptic straw of 3 1ml-4、10-5、10-6The dilution each 1ml of bacteria suspension, puts in corresponding sterile petri dish.
The defect of the method is, needs to prepare the substantial amounts of test tube filling 9ml sterilized water and aseptic suction pipe, needs to use in operating process
Oral cavity is inhaled or is blown and carries out, and operates the most troublesome, time-consuming.
It addition, CN 104266895 A(bacteria suspension continuous multi-stage automatic dilution device and real-time counting method) also public
Having opened a kind of apparatus and method, but the defect of this device and method is, apparatus structure assembly complicated, each is connected by pipeline,
It uses " radiation mode carries out sterilizing ", and cannot be carried out the high temperature sterilize of full device, thus may at each assembly or
Produce dead angle, cooperating microorganisms in pipeline, thus affect dilution or the accuracy of counting.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of microorganism air mix facilities, it is possible to reduce aseptic straw
Use, make operating process simplify and ensure diluted concentration precision.
For solving above-mentioned technical problem, this utility model be the technical scheme is that
A kind of microorganism air mix facilities, including syringe, syringe lower end is provided with inlet and liquid outlet, inlet and
The first flexible pipe and the second flexible pipe, first check valve one end and the first hose connection, the other end and suction it is connected on liquid outlet
Pipe connects, and second check valve one end is hose connection with second, the other end is connected with comb；Described first check valve only allows liquid
Entering syringe, the second check valve only allows liquid to discharge from syringe.
Described syringe is provided with scale.
Dish (12) laid by the supporting test tube that is provided with in described comb (9) lower section, and including base (13), bearing is passed through in rotating shaft (14)
Being vertically installed on base (13), rotating shaft (14) is arranged over support (15), and support (15) places circle at least provided with six
(16).
This utility model one microorganism air mix facilities, has following technical effect that
1) the aseptic formula, utilizing the first check valve and the second check valve to complete liquid sucks mixing continuously and discharges dilution.
Simplify microorganism dilution operating procedure, decrease the usage amount of aseptic straw, save the operating time of 2/3, improve work
Make efficiency；
2), utilize syringe, make in operating process device without and oral contact, improve the safety of operator；
3), due to whole dilution operation in all be use same double syringe so that compared with traditional method use some
The error that suction pipe operation is brought greatly reduces, and improves the accuracy of measurement.
4), this apparatus structure simple, the sterilizing of full device can be carried out, stopped sample and the most thoroughly carried due to utensil sterilizing
The measurement error come.
Accompanying drawing explanation
The utility model is described in further detail with embodiment below in conjunction with the accompanying drawings:
Fig. 1-2 is schematic diagram of the present utility model.
In figure: syringe 1, inlet 2, liquid outlet 3, the first flexible pipe 4, the second flexible pipe 5, the first check valve 6, inhale
Pipe 7, the second check valve 8, comb 9, triangular flask 10, test tube 11.
Detailed description of the invention
As it is shown in figure 1, a kind of microorganism air mix facilities, including syringe 1, syringe 1 lower end is provided with inlet 2 and goes out
The first flexible pipe 4 and the second flexible pipe 5, first check valve 6 one end and first it is connected on liquid mouth 3, inlet 2 and liquid outlet 3
Flexible pipe 4 connects, the other end is connected with suction pipe 7, and second check valve 8 one end is connected with the second flexible pipe 5, the other end is connected with comb 9；
6, described first check valve allows liquid to enter syringe 1, and the second 8, check valve allows liquid to discharge from syringe 1.
Described syringe 1 is provided with scale.Thus can conveniently observe injection rate.
Below described comb 9, dish 12 laid by the supporting test tube that is provided with, and including base 13, rotating shaft 14 is vertically-mounted by bearing
On base 13, rotating shaft 14 is arranged over support 15, and support 15 places circle 16 at least provided with six.When experiment, enter in advance
Line flag, is then directly placed on test tube by test tube and lays in dish 12, often injected successively, Rotatable bracket 15, makes the nothing of sky
Bacterium test tube 11 is the most right with comb 9, injects next time.
Operation principle and process:
1), first, the push handle on syringe 1 is pulled, by microbial bacteria suspension to be diluted for 1ml from aseptic inlet 2
Suck.
2), then, be sequentially connected with on inlet 2 in the way of sterile working first flexible pipe the 4, first check valve 6 and
Suction pipe 7, is sequentially connected with the second flexible pipe the 5, second check valve 8 and comb 9 on liquid outlet 3.
3), secondly, suction pipe 7 is put under the sterilized water liquid level of triangular flask 10, pulls the push handle on syringe 1,
First check valve 6 is opened, and the second check valve 8 closes, and sterilized water is drawn in injection tube from triangular flask 10, to injection tube
In liquid level reach stopping action when of 10ml, make sterile liquid mix homogeneously with microbial bacteria suspension；Then put at comb 9
Putting sterile test tube 11, the push handle on pushing syringe 1, the first check valve 6 closes, and the second check valve 8 is opened, and 9ml bacteria suspension is from comb 9
It is drained in test tube 11, thus obtains the 10 of 9ml-1Microbial cells diluent, syringe 1 also leaves the 10 of 1ml-1
Bacteria suspension.
4), repeat 3) step, i.e. available different dilution factors are (such as: 10-2、10-3、10-4...) microbial bacteria hang
Liquid.
The amount of the amount of the microbial bacteria suspension to be diluted during aforesaid operations and the sterilized water of addition can be according to reality
Situation is adjusted (as the amount of microbial bacteria suspension to be diluted is adjusted to 0.5ml, sucking from triangular flask 10 every time
The sterilized water of 4.5ml is in injection tube), as long as ensureing that bacteria suspension becomes 10-nDilution again.
Claims (3)
1. a microorganism air mix facilities, it is characterised in that: include that syringe (1), syringe (1) lower end are provided with inlet (2)
Be connected to the first flexible pipe (4) and the second flexible pipe (5) on liquid outlet (3), inlet (2) and liquid outlet (3), first is unidirectional
Valve (6) one end is connected with the first flexible pipe (4), the other end is connected with suction pipe (7), the second check valve (8) one end and the second flexible pipe (5)
Connect, the other end is connected with comb (9)；Described first check valve (6) only allows liquid to enter syringe (1), the second check valve
(8) liquid is only allowed to discharge from syringe (1).
A kind of microorganism air mix facilities the most according to claim 1, it is characterised in that: described syringe (1) is provided with quarter
Degree.
A kind of microorganism air mix facilities the most according to claim 1, it is characterised in that: described comb (9) lower section is supporting to be set
Being equipped with test tube and lay dish (12), including base (13), rotating shaft (14) is vertically installed on base (13) by bearing, rotating shaft (14)
It is arranged over support (15), support (15) places circle (16) at least provided with six.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620062454.6U CN205538417U (en) | 2016-01-22 | 2016-01-22 | Microorganism diluting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620062454.6U CN205538417U (en) | 2016-01-22 | 2016-01-22 | Microorganism diluting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205538417U true CN205538417U (en) | 2016-08-31 |
Family
ID=56768377
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620062454.6U Expired - Fee Related CN205538417U (en) | 2016-01-22 | 2016-01-22 | Microorganism diluting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205538417U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111992130A (en) * | 2020-08-11 | 2020-11-27 | 铜仁市第二人民医院 | Liquid adding and diluting device for medical clinical laboratory |
-
2016
- 2016-01-22 CN CN201620062454.6U patent/CN205538417U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111992130A (en) * | 2020-08-11 | 2020-11-27 | 铜仁市第二人民医院 | Liquid adding and diluting device for medical clinical laboratory |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160831 Termination date: 20170122 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |