CN205483726U - Experiment mouse is kidney section mould roughly - Google Patents
Experiment mouse is kidney section mould roughly Download PDFInfo
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- CN205483726U CN205483726U CN201620114144.4U CN201620114144U CN205483726U CN 205483726 U CN205483726 U CN 205483726U CN 201620114144 U CN201620114144 U CN 201620114144U CN 205483726 U CN205483726 U CN 205483726U
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Abstract
The utility model relates to an experiment mouse is kidney section mould roughly. Experiment mouse is kidney section mould roughly be the cuboid shape, the recess that a size according to the mouse kidney, shape were seted up is seted up on its surface, the shape of recess is described or oblong shape for the young silkworm beans, following sword groove can be opened in the surface of mould: along being on a parallel with the length direction of recess has seted up and has run through the multichannel sword groove of recess, perhaps along the perpendicular to the length direction of recess has seted up multichannel sword groove, just is in solitary sword groove is together seted up to the vertical direction of the half -way point in multichannel sword groove. The utility model discloses the mouse of being applied to separation among the medical experiment tissue of three kinds of section directions at nephridial tissue coronal section, sagittal face and the horizontal plane is roughly drawn materials and prepares the meticulous section of nephridial tissue, make the pathologic sampling of nephridial tissue more simple and convenient, rapid, complete, has reduced the form that the nephridial tissue caused because of extrusion and cutting at the in -process of drawing materials and the change of structure.
Description
Technical field
This utility model relates to a kind of experiment mould, particularly relates to a kind of experiment mice substantially kidney segment mould.
Background technology
Along with the development of advancing by leaps and bounds of medical biotechnology science, in order to obtain more new knowledge about the aspect such as biology, medical science, to use animal to carry out the scope of scientific research more and more extensive.The importance of laboratory animal is more and more recognized by people, advanced country's development to Laboratory Animal Science, gives the attention of height, almost can mention in the same breath with development atomic energy science, and its significance is well imagined.Laboratory Animal Science is the important component part of modern science and technology, is basis and the condition of life sciences, is to weigh a country or the important symbol of R&D institution's scientific research level.In biomedical sector, laboratory animal act as safety test, effect test, the role of code test.
Mice is one of laboratory animal of the most often selecting of biomedical sector.The length of its gene-code chain is very nearly the same with the mankind, has 2,900,000,000 pairs of nucleotide of 2,500,000,000 pairs of nucleotide, the slightly less than mankind.The human gene of 80% is identical with mice, and the human gene of 99% is closely similar with mice, and the disease that a lot of mankind are difficult to cure can find similar character with mice, is more easy to discovery by experiment and cures the disease gene.The breeding method testing special mouse inbred lines has formed fixing flow process through a century exploration, and the mice cultivated is almost complete absence of physiologically individual variation.Artificial propagation abundant amount, can meet many experiment needs statistical analysis.The advantages such as mice has that germline is pure, is mammal, the bodily form is little, artificial propagation is fed easily, low price become one of laboratory animal of the most often selecting in nephrology field equally.People can be by ligation, and the means such as excision, gene knockout make model and the researchs to nephrotoxicity of the various medicine such as acute and chronic renal failure, diabetic nephropathy, obstructive nephropathy, hypertensive nephropathy, tumor of kidney, blood vessel embolism to mice.People can also realize kidney three-dimensional reconstruction by the picture of computer tangent plane each on kidney of mouse and study the impact that kidney is caused by contusion of kidney.Drawing materials and cutting into slices of nephridial tissue is the basis of tissue general structure and Ultrastructural observation.
Fresh nephridial tissue is relatively soft, in tissue, contained liquid is more, in irregular shape it is difficult to accomplish the thin slice cutting out 1mm uniform, neat, complete in the case of not crushing tissue, and the wafer cutting die of existing mouse brain and Kidney Size, structure, shape is different is not used to renal tissue section.Therefore, it is necessary to be developed for the wafer cutting die of the substantially kidney of experiment mice.
Summary of the invention
The purpose of this utility model is to provide a kind of experiment mice substantially kidney segment mould, for preparing the fine section of the tissue sampling of mice in vitro in medical experiment substantially nephridial tissue coronalplane, sagittal plane and three kinds of slice directions of horizontal plane.
Experiment mice described in the utility model substantially kidney segment mould is rectangular shape, the groove that a size according to mouse kidney is offered on its surface, shape is offered；Described groove be shaped as small broad bean shape or oblong；Following cutter groove can be opened in the surface of described mould: offer the multiple tracks cutter groove running through described groove along the length direction being parallel to described groove；Or offer multiple tracks cutter groove along the length direction being perpendicular to described groove, and offer the most individually cutter groove in the vertical direction of the intermediate point of described multiple tracks cutter groove.
According to first detailed description of the invention of experiment mice described in the utility model substantially kidney segment mould, described mould is rectangular shape, the groove that a size according to mouse kidney is offered on its surface, shape is offered；Described groove be shaped as small broad bean shape；The surface of described mould offers the multiple tracks cutter groove running through described groove along the length direction being parallel to described groove.
Preferably, the spacing between the adjacent cutter groove of described multiple tracks cutter groove is 1 mm so that slice thickness is accurate to 1 mm.
Preferably, the quantity of described cutter groove is 8 roads.
Preferably, described groove is opened in the central authorities on surface of described mould.
According to second detailed description of the invention of experiment mice described in the utility model substantially kidney segment mould, described mould is rectangular shape, the groove that a size according to mouse kidney is offered on its surface, shape is offered；Described groove be shaped as small broad bean shape；The surface along perpendicular of described mould offers multiple tracks cutter groove in the length direction of described groove, and offers the most individually cutter groove in the vertical direction of the intermediate point of described multiple tracks cutter groove.
Preferably, the spacing between the adjacent cutter groove of described multiple tracks cutter groove is 1 mm so that slice thickness is accurate to 1 mm.
Preferably, the quantity of described cutter groove is 16 roads.
Preferably, described groove is opened in the central authorities on surface of described mould.
The 3rd detailed description of the invention according to experiment mice described in the utility model substantially kidney segment mould, described mould is rectangular shape, the groove that a size according to mouse kidney is offered on its surface, shape is offered；Described groove be shaped as oblong；The surface along perpendicular of described mould offers multiple tracks cutter groove in the length direction of described groove, and offers the most individually cutter groove in the vertical direction of the intermediate point of described multiple tracks cutter groove.
Preferably, the spacing between the adjacent cutter groove of described multiple tracks cutter groove is 1 mm so that slice thickness is accurate to 1 mm.
Preferably, the quantity of described cutter groove is 16 roads.
Preferably, described groove is opened in the central authorities on surface of described mould.
This utility model is drawn materials a set of three kinds of moulds of mode and angle design according to professional's the most frequently used mice substantially nephridial tissue totally, and it is mainly used in mice in vitro in medical experiment substantially nephridial tissue coronalplane, sagittal plane and the tissue sampling of three kinds of slice directions of horizontal plane and preparation nephridial tissue is finely cut into slices.Experiment mice described in the utility model substantially kidney segment mould makes softness, in irregular shape, fresh mice holonephros tissue cut out uniform 1mm thin slice, it is easy to observe the pathological change of the every aspects such as kidney of mouse tumor, cyst, blood vessel embolism and contusion of kidney, the pathologic sampling making nephridial tissue is easier, rapid, complete, decreases form and the change of structure that nephridial tissue causes during drawing materials because of extruding and cutting.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of first detailed description of the invention of experiment mice described in the utility model substantially kidney segment mould.
Fig. 2 is the schematic diagram of second detailed description of the invention of experiment mice described in the utility model substantially kidney segment mould.
Fig. 3 is the schematic diagram of the 3rd detailed description of the invention of experiment mice described in the utility model substantially kidney segment mould.
Detailed description of the invention
In conjunction with specific embodiments this utility model is described in detail below according to accompanying drawing.
Term is explained:
Sagittal plane: refer to fore-and-aft direction, is divided into left and right two-part longitudinal section by human body, and this tangent plane is vertical with ground level.Sagittal plane through human body center is median sagittal plane, and human body is divided into two parts that left and right is equal by this face.
Coronalplane: refer to left and right directions, two-part longitudinal section before and after being divided into by human body, this tangent plane is mutually perpendicular to sagittal plane and horizontal plane.
Horizontal plane: also referred to as cross section, is plane that human body be divided into upper and lower two parallel with ground level, and this plane is mutually perpendicular to coronalplane and sagittal plane.
Embodiment 1: experiment mice described in the utility model substantially kidney segment mould (sagittal plane mould)
Experiment mice described in the utility model substantially kidney segment mould, the groove 2 that as it is shown in figure 1, described mould 1 is rectangular shape, the central authorities on its surface offer a size according to mouse kidney, shape is offered.This groove 2 be shaped as small broad bean shape so that the Ren Mus coronalplane put into is upwards.The surface of this mould 1 offers the 8 road cutter grooves 3 running through described groove along the length direction being parallel to described groove.Spacing between the adjacent cutter groove of these cutter grooves is 1 mm so that slice thickness is accurate to 1 mm.
Embodiment 2: experiment mice described in the utility model substantially kidney segment mould (horizontal plane and sagittal plane wafer cutting die)
Experiment mice described in the utility model substantially kidney segment mould, the groove 2 that as in figure 2 it is shown, described mould 1 is rectangular shape, the central authorities on its surface offer a size according to mouse kidney, shape is offered.This groove 2 be shaped as small broad bean shape so that the Ren Mus coronalplane put into is upwards.The surface along perpendicular of this mould 1 offers 16 road cutter grooves 3 in the length direction of groove 2.Spacing between the adjacent cutter groove of these cutter grooves is 1 mm so that slice thickness is accurate to 1 mm.And, the vertical direction at the intermediate point of these cutter grooves 3 offers the most individually cutter groove 4.
Embodiment 3: experiment mice described in the utility model substantially kidney segment mould (horizontal plane and coronalplane wafer cutting die)
Experiment mice described in the utility model substantially kidney segment mould, the groove 2 that as it is shown on figure 3, described mould 1 is rectangular shape, the central authorities on its surface offer a size according to mouse kidney, shape is offered.This groove 2 be shaped as oblong so that the Ren Mus sagittal plane put into is upwards.The surface along perpendicular of this mould 1 offers 16 road cutter grooves 3 in the length direction of groove 2.Spacing between the adjacent cutter groove of these cutter grooves is 1 mm so that slice thickness is accurate to 1 mm.And, the vertical direction at the intermediate point of these cutter grooves 3 offers the most individually cutter groove 4.
When specifically making, mould is that import rustless steel makes, precision optical machinery processing and high polish, stand scouring, heat, cool down, the use condition such as autoclaving, indeformable, ruggedness, favorable repeatability.The degree of accuracy of this mould is high, and slice thickness is accurate to 1mm, and common razors slice can be used to cut into slices, it is possible to use special tissue section knife section, consumptive material is simple and easy to get.
The shape of mould and size are the maximum settings through 20 ill Ren Mus wax-patterns, and design up big and down small in groove both can have been fixed the kidney of enlargement and the most properly fix normal Ren Mus.Mode and the professional that is of microtome angle that tissue is put are provided.Groove between the edge of a knife is streamlined excessively to the next edge of a knife, and each edge of a knife and groove are all measured through fine, polishing grinding, takes out the damage to nephridial tissue in organizational process after reducing section as far as possible.
In a specific embodiment, mould is rectangular-shaped, and gross dimensions is long 4.5cm × wide 2.7cm × high 2.2cm.Size according to Ren Mus, shape offer placement Ren Mus groove (abbreviation groove).Groove is opened in the face center portion in cuboid length and width face (4.5cm × 2.7cm face), the major axis of longer axis parallel mould.A mould (embodiment 1), the groove shapes of B mould (embodiment 2) are " small broad bean shape " (Ren Mus coronalplane is upwards), and the groove shapes of C mould (embodiment 3) is oblong (Ren Mus sagittal plane is upwards).A mould offers the cutter groove that 9 roads that be parallel to length direction, that run through described groove insert for tissue section knifes in the middle part of described body surface, and it is made for Mouse Kidney sagittal plane 0.1cm tissue slice.B mould in described body surface 1/3 offer cutter groove (be called for short multitool groove) that be perpendicular to length direction, that run through described groove Gong 16 road and insert for tissue section knife and on the vertical line of multitool groove intermediate point, offered the cutter groove (being called for short hilted broadsword groove) inserted for tissue section knife together.What this mould multitool groove direction made is mice horizontal plane 0.1cm tissue slice, and what hilted broadsword groove direction made is the segmentation of Mouse Kidney sagittal plane.The mode of the operated on groove of C mould is identical with B mould.What multitool groove direction made is Mouse Kidney horizontal plane 0.1cm tissue slice, and hilted broadsword groove direction is the segmentation of mice coronalplane.Three kinds of mould all of cutter groove depth, width are uniform sizes, and width is 0.33cm, and the degree of depth is 0.6cm, and multitool groove each cutter groove interval width is 0.1cm.Can use the section of common razors slice or special tissue section knife by each enter knife-edge part slowly put into, carry out the section of all angles.
Experiment mice kidney segment mold use described in the utility model illustrates:
Prepare before section: with the 75% whole mould of alcohol wipe before section, cleaning surface contaminants and bloodstain, with the pollutant in spicule cleaning edge gap, through autoclaving sterilization.Prepare section disposable blade, and disinfect in alcohol.
During section: mould is placed on slab, groove is upwards, kidney of mouse is put into by the shape of groove, choose tissue to put and slice direction, determine position of need to cutting into slices, it is perpendicular to microtome knife groove, microtome knife (using the sharpest razor or special tissue slice blade) is slowly inserted along edge of a knife groove in first cutter groove of identification, ready second blade is inserted second adjacent cutter groove.Take out first blade and can take first tissue slice out of.The a piece of blade stayed in second cutter groove is stable for ensureing piece of tissue structure and position, continues to be inserted simultaneously into two blades afterwards and can obtain second tissue slice in adjacent section groove.Repeat above operation, until the section work of desired area is fully completed.Rapidly tissue is put into embedded box carry out mark and tissue is fixed process.
After section: tap water cleans mould, the tissue remained in cleaning groove and bloodstain, dry for standby in 37 DEG C of baking boxs.
Claims (3)
1. an experiment mice substantially kidney segment mould, it is characterised in that: described mould is rectangular shape, and its surface offers one
The groove that the individual size according to mouse kidney, shape are offered；Described groove be shaped as small broad bean shape or oblong；Described mould
Following cutter groove can be opened in the surface of tool: offer the multiple tracks running through described groove along the length direction being parallel to described groove
Cutter groove.
Experiment mice the most according to claim 1 substantially kidney segment mould, it is characterised in that: described multiple tracks cutter groove adjacent
Spacing between cutter groove is 1 mm.
Experiment mice the most according to claim 1 substantially kidney segment mould, it is characterised in that: the quantity of described cutter groove is 8
Road.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620114144.4U CN205483726U (en) | 2016-02-05 | 2016-02-05 | Experiment mouse is kidney section mould roughly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620114144.4U CN205483726U (en) | 2016-02-05 | 2016-02-05 | Experiment mouse is kidney section mould roughly |
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| Publication Number | Publication Date |
|---|---|
| CN205483726U true CN205483726U (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620114144.4U Expired - Fee Related CN205483726U (en) | 2016-02-05 | 2016-02-05 | Experiment mouse is kidney section mould roughly |
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| Country | Link |
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| CN (1) | CN205483726U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353127A (en) * | 2016-10-28 | 2017-01-25 | 广东省中医院 | Synchronous slicing device for tissues |
| CN107727463A (en) * | 2017-09-18 | 2018-02-23 | 南方医科大学珠江医院 | The method that the preparation of super thick histotomy and cellular level reproduce tissue three-dimensional morphosis |
-
2016
- 2016-02-05 CN CN201620114144.4U patent/CN205483726U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353127A (en) * | 2016-10-28 | 2017-01-25 | 广东省中医院 | Synchronous slicing device for tissues |
| CN107727463A (en) * | 2017-09-18 | 2018-02-23 | 南方医科大学珠江医院 | The method that the preparation of super thick histotomy and cellular level reproduce tissue three-dimensional morphosis |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20190205 |
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| CF01 | Termination of patent right due to non-payment of annual fee |