CN205398640U - Detecting system is united to single biological sample multi -parameter - Google Patents
Detecting system is united to single biological sample multi -parameter Download PDFInfo
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- CN205398640U CN205398640U CN201620153260.7U CN201620153260U CN205398640U CN 205398640 U CN205398640 U CN 205398640U CN 201620153260 U CN201620153260 U CN 201620153260U CN 205398640 U CN205398640 U CN 205398640U
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Abstract
The utility model discloses a detecting system is united to single biological sample multi -parameter, characterized by by the sample select separately the unit, catch detecting element, control by temperature change unit and data analysis unit constitute, waiting to examine biological sample and at first selecting separately the unit through the sample and obtain each respectively and detect appearance, each detects appearance and gets into and catch detecting element, utilize catch among the detecting element respectively catch the pipeline accomplish respectively to each detect catching of in appearance purpose composition, and/or pour into the correlated response that the purpose composition was accomplished to relevant reagent into in catching the pipeline, obtain to detect data. The utility model discloses can be simultaneously to same sample, even the multi -parameter integration of carrying out cell, gene and protein level of same kind cell jointly detect.
Description
Technical field
This utility model relates to a kind of clinical medicine specimen multi objective combined detection system, the more specifically cell of a kind of single creature sample, albumen and gene multiparameter joint-detection device.
Background technology
The examination of a lot of diseases and diagnosis clinically, including neonate defect, lacks a kind of not only special but also sensitive index mostly, there is the risk failed to pinpoint a disease in diagnosis with mistaken diagnosis, adopt the joint-detection of multiple index, the deficiency of single index can be made up to a certain extent, improve diagnosis efficiency.
Along with developing rapidly of flow technique, micro electro mechanical system (MEMS) technology, molecular biology and immunology and detection technique thereof, the visible components such as cell in biological specimen can be carried out initial gross separation by physical method by scientific research personnel, and is caught by immunolabelling technique and detect cell therein and albumen.By the procedural temperature cycles accurately controlled, it is achieved gene amplification, greatly improve the sensitivity of detection.But, the separation of sample, labelling, cell detection, Protein Detection and gene test are substantially separately performed by existing experimental technique, take that the method not only amount of samples that multiple index detects one by one is big, time-consuming, overall expenses is high, and cannot realize that single sample is included single celled multi objective and detect, significantly limit the raising of diagnosis efficiency, it is impossible to meet the demand of precisely medical treatment.
Utility model content
This utility model is the deficiency for avoiding above-mentioned prior art to exist, the cell of a kind of single creature sample, albumen and gene multiparameter joint-detection device are provided, to realize the multiparameter joint-detection with a specimen or the wherein multi objective of cell lineage, gene and albumen aspect, it is beneficial to improve disease, including examination and the diagnosis efficiency of birth defect.
This utility model adopts the following technical scheme that for solving technical problem
The feature of this utility model single creature sample multiparameter combined detection system is: described detection system is made up of sample separation unit, Acquisition Detection unit, temperature conditioning unit and data analysis unit；Biological specimen to be checked first passes around described sample separation unit and obtains each detection sample respectively, described each detection sample enters Acquisition Detection unit, the pipeline of respectively catching in described Acquisition Detection unit is utilized to be respectively completed the catching of purpose composition in each detection sample, and/or pipeline injects related reagent complete the correlated response of purpose composition described catching, it is thus achieved that detection data；Utilize described temperature control system to control the reaction temperature of described correlated response, described data analysis unit described detection data are analyzed obtaining experimental data；The correlated response of described purpose composition refers to the correlated response that the character by purpose composition and detection method are arranged.
The feature of this utility model single creature sample multiparameter combined detection system lies also in:
Pipeline of respectively catching in described Acquisition Detection unit is first catch pipeline, second catch pipeline, the 3rd catch pipeline, the 4th catch pipeline, the 5th catch pipeline and the 6th and catch pipeline respectively；
Described sample separation unit has sample pool, and biological specimen to be checked is infused in described sample pool；
Described sample pool one tunnel directly communicates with first passage node, and another Lu Jingzhi pipe filter communicates with second channel node；Described first passage node is configured respectively catches the detection sample stop valve and first of pipeline porch in correspondence and catches pipeline, second catches pipeline and the 3rd and catch pipeline and communicate；Described second channel node is configured respectively catches the detection sample stop valve and the 4th of pipeline porch in correspondence and catches pipeline, the 5th catches pipeline and the 6th and catch pipeline and communicate；
Each reagent and reagent stop valve are set, are the first reagent communicated with third channel node by the first reagent stop valve respectively, and communicated with fourth lane node the second reagent by the second reagent stop valve；
Being respectively provided with waist valve in the described outlet respectively catching pipeline, each waist valve communicates with waste liquid pool through sewer pipe, arranges Miniature liquid pump in described sewer pipe, and by described Miniature liquid pump, for sample and reagent, the directed flow in pipeline provides negative pressure driving force.
The feature of this utility model single creature sample multiparameter combined detection system lies also in:
Sample stop valve is detected on described first passage node one tunnel first and the first entrance catching pipeline communicates, sample stop valve is detected on another road second and the second entrance catching pipeline communicates, and another Lu Jing tri-detects sample stop valve and the 3rd entrance catching pipeline communicates；
Described second channel node one tunnel the 4th detection sample stop valve and the 4th entrance catching pipeline communicate, another Lu Jing five detects sample stop valve and the 5th entrance catching pipeline communicates, and another Lu Jing six detects sample stop valve and the 6th entrance catching pipeline communicates；
Described third channel node is to communicate through the first reagent stop valve and the 3rd entrance catching pipeline, and fourth lane node is to communicate through the second reagent stop valve and the 4th entrance catching pipeline.
The feature of this utility model single creature sample multiparameter combined detection system lies also in: described in catch pipeline and refer to that being coated with specific antibody on inner-walls of duct in advance catches pipeline for the cell catching in sample respectively, albumen or free nucleic acid；Described pipeline of catching can also include grappling in advance in vias inner walls and has oligonucleotide probe to catch pipeline for what catch the acid of sample middle reaches freestones.
The feature of this utility model single creature sample multiparameter combined detection system lies also in: respectively catch the detection sample stop valve of pipeline porch, reagent stop valve by turning off, and turn off the waist valve in the exit respectively catching pipeline, make pipeline to form airtight reaction chamber respectively catching, utilize temperature conditioning unit that each reaction chamber carries out independent temperature to control, respectively constitute cell detection district, Protein Detection district and gene test district.
The feature of this utility model single creature sample multiparameter combined detection system lies also in: arrange temperature control console, and be arranged in parallel each road temperature control groove on the top table top of described temperature control console；Described pipeline of catching is pipe fitting, described pipeline one_to_one corresponding of catching is inlaid in described temperature control groove, utilize described temperature control groove to control to be formed the temperature of reaction chamber in catching pipeline, at described temperature control console and catch and be covered with heat-conducting glue between pipeline, be used for bonding temperature control console and catching pipeline.
Compared with the prior art, this utility model technique effect is embodied in:
1, this utility model system adopts liquid stream control, antigen antibody reaction, nucleic acid hybridization technique to separate, catch special component in biological specimen, realize specific cells, albumen and gene are carried out multiparameter joint-detection, and catch pipeline because of detection applications difference tube chamber endoperidium antibody or anchor probe, removable, it is possible to carry out different combinations according to the difference of various disease diagnosis marker；
2, this utility model utilizes tube chamber endoperidium to have the pipeline of specific antibody and nucleic probe, catch specific components in the biological sample by liquid flow control technology initial gross separation, integrated with temperature control system, every is caught pipeline and implements accurate temperature circulation and thermostatic control, gene amplification, gene recombination, integrated enzyme reaction and enzymatic reaction etc. are carried out to catching component according to character different choice, it is achieved detect while the multiple parameter of cell lineage, gene and albumen aspect catching original position in pipeline；
3, in this utility model, tube chamber endoperidium has the difference of specific antibody and nucleic probe to catch pipeline, cell in sample, albumen, free nucleic acid can be caught respectively simultaneously, reaction chamber is formed by Valve controlling, under the control of integrated temperature control system, carry out gene amplification, gene recombination, integrated enzyme reaction and enzymatic reaction etc., and then components multiple in single creature sample can be carried out gene and the detection of albumen aspect；
4, it is embedded for catching pipeline in this utility model, it is possible to displacement, and according to the difference of testing goal and method, can carry out that antibody is coated, probe grappling etc. processes in advance to catching inner cavity of pipe, composition various disease examination and diagnosis combined complete；
5, this utility model is to arrange stop valve to constitute reaction chamber, its simple in construction at the two ends catching pipeline, it is easy to implement.
6, this utility model carries out independent temperature control by temperature control system to respectively catching pipeline, makes testing result more accurate.
Accompanying drawing explanation
Fig. 1 is this utility model system structure schematic diagram；
Fig. 2 is temperature control console section structure schematic diagram in this utility model；
Fig. 3 is temperature control console facade structures schematic diagram in this utility model；
Number in the figure: 11 sample pools, 12 straight-through branch roads, 13 pipe filters, 15 waste liquid pools, 14 Miniature liquid pumps, 21 first catch pipeline, 22a the first reagent, 22b the second reagent, 22 second catch pipeline, and 23 the 3rd catch pipeline, 24 the 4th catch pipeline, and 25 the 5th catch pipeline, and 26 the 6th catch pipeline, A first passage node, B second channel node, C third channel node, D fourth lane node, 31 temperature control console, 32 heat-conducting glues.
Detailed description of the invention
Referring to Fig. 1, Fig. 2 and Fig. 3, in the present embodiment, single creature sample multiparameter combined detection system is made up of sample separation unit, Acquisition Detection unit, temperature conditioning unit and data analysis unit；Biological specimen to be checked first passes around sample separation unit and obtains each detection sample respectively, each detection sample enters Acquisition Detection unit, the pipeline of respectively catching in Acquisition Detection unit is utilized to be respectively completed the catching of purpose composition in each detection sample, and/or injection related reagent completes the correlated response of purpose composition in catching pipeline, it is thus achieved that detection data；Utilize temperature control system to control the reaction temperature of correlated response, data analysis unit detection data are analyzed obtaining experimental data；The correlated response of purpose composition refers to the correlated response that the character by purpose composition and detection method are arranged.
In being embodied as, corresponding structure arranges and includes:
Pipeline of respectively catching in Acquisition Detection unit is first catch pipeline 21, second catch pipeline the 22, the 3rd and catch pipeline the 23, the 4th and catch pipeline the 24, the 5th and catch pipeline 25 and the 6th and catch pipeline 26 respectively；Sample separation unit has sample pool 11, and biological specimen to be checked is infused in sample pool 11.
As it is shown in figure 1, sample pool 11 is leaded up to straight-through branch road 12 and directly communicated with first passage node A, another Lu Jingzhi pipe filter 13 communicates with second channel node B；First passage node A is configured respectively to be caught the detection sample stop valve and first of pipeline porch in correspondence and catches pipeline 21, second catches pipeline 22 and the 3rd and catch pipeline 23 and communicate；Second channel node B is configured respectively to be caught the detection sample stop valve and the 4th of pipeline porch in correspondence and catches pipeline the 24, the 5th and catch pipeline 25 and the 6th and catch pipeline 26 and communicate.
Shown in Fig. 1, each reagent and reagent stop valve are set, are by the first reagent stop valve and third channel node C the first reagent 22a communicated respectively, and communicated with fourth lane node D the second reagent 22b by the second reagent stop valve；Being respectively provided with waist valve in the outlet respectively catching pipeline, each waist valve communicates with waste liquid pool 15 through sewer pipe, arranges Miniature liquid pump 14 in sewer pipe, and by Miniature liquid pump 14, for sample and reagent, the directed flow in pipeline provides negative pressure driving force.
In the present embodiment, pipeline is caught for six tunnels, sample stop valve is detected on first passage node A mono-tunnel first and the first entrance catching pipeline 21 communicates, sample stop valve is detected on another road second and the second entrance catching pipeline 22 communicates, and another Lu Jing tri-detects sample stop valve and the 3rd entrance catching pipeline 23 communicates；Second channel node B mono-tunnel the 4th detection sample stop valve and the 4th entrance catching pipeline 24 communicate, another Lu Jing five detects sample stop valve and the 5th entrance catching pipeline 25 communicates, and another Lu Jing six detects sample stop valve and the 6th entrance catching pipeline 26 communicates；Third channel node C catches the entrance of pipeline 23 and the 4th entrance catching pipeline 24 communicates through the first reagent stop valve and the 3rd, and fourth lane node D catches the entrance of pipeline 23 and the 4th entrance catching pipeline 24 communicates through the second reagent stop valve and the 3rd.
Catch pipeline and refer to that being coated with specific antibody on inner-walls of duct in advance catches pipeline for the cell catching in sample respectively, albumen or free nucleic acid；Catch pipeline to include grappling in advance in vias inner walls and have oligonucleotide probe to catch pipeline for what catch the acid of sample middle reaches freestones.
In detection process, the detection sample stop valve of pipeline porch, reagent stop valve is respectively caught by turning off, and turn off the waist valve in the exit respectively catching pipeline, make pipeline to form airtight reaction chamber respectively catching, utilize temperature conditioning unit that each reaction chamber carries out independent temperature to control, respectively constitute cell detection district, Protein Detection district and gene test district.
As shown in Figures 2 and 3, arranging temperature control console 31 in the present embodiment, be arranged in parallel each road temperature control groove on the top table top of temperature control console 31；Catching pipeline is pipe fitting, catch pipeline one_to_one corresponding and be inlaid in temperature control groove, utilize temperature control groove to control to be formed the temperature of reaction chamber in catching pipeline, at temperature control console and catch and be covered with heat-conducting glue 32 between pipeline, be used for bonding temperature control console and catching pipeline.
During this utility model is specifically applied, the cell suspension etc. that biological specimen to be checked and specimen include blood, urine, ascites pleural fluid, cerebrospinal fluid, sputum, exfoliative cyte, amniotic fluid, piece of tissue are made, specimen containing cell component respectively enters through straight-through branch road 12 and first catches pipeline 21, second catches pipeline 22 and the 3rd and catch pipeline 23, and wherein specific cells is caught by specific antibody and is adsorbed on the tube wall catching pipeline；Specimen is after a pipe filter 13, the visible components such as cell therein are filtered, the compositions such as free nucleic acid, albumen, polypeptide enter follow-up the 4th and catch pipeline the 24, the 5th and catch pipeline 25 and the 6th and catch pipeline 26, utilize the probe of coated antibody or grappling in advance that specific molecular is caught；In specimen specific cells or specific molecular captured after, according to requirement not, pipeline is injected separately into mix reagent or single agents carries out correlated response respectively catching；Nucleic acid amplification, nucleic acid hybridization, integrated enzyme reaction, enzymatic reaction carry out in different catching in pipeline simultaneously, carry out independent scan, opto-electronic conversion and analysis by the optical signalling that differential responses are produced and obtain testing result.
Difference according to testing goal and method, is coated specific antibody or grappling specific probe in advance catching inner-walls of duct.According to examination and the difference that diagnoses the illness, these prefabricated can be caught pipeline composition diagnosis suit, for circulating tumor cell detection in blood and in Identification of The Origin, sputum in tumor cell, ascites pleural fluid in tumor cell, detachment of cervix cell, cerebrospinal fluid tumor cell, mother's peripheral blood fetal nucleated red blood and amniocyte for special pathogen, albumen, free detection of nucleic acids etc. in birth defect diagnosis and various body fluid.
In being embodied as, configuration optical analyser realizing application of sample, sort, catch, react, detect, analyze, send the overall process reported and automatically control.
Claims (6)
1. a single creature sample multiparameter combined detection system, is characterized in that: described detection system is made up of sample separation unit, Acquisition Detection unit, temperature conditioning unit and data analysis unit；Biological specimen to be checked first passes around described sample separation unit and obtains each detection sample respectively, described each detection sample enters Acquisition Detection unit, the pipeline of respectively catching in described Acquisition Detection unit is utilized to be respectively completed the catching of purpose composition in each detection sample, and/or pipeline injects related reagent complete the correlated response of purpose composition described catching, it is thus achieved that detection data；Utilize described temperature control system to control the reaction temperature of described correlated response, described data analysis unit described detection data are analyzed obtaining experimental data；The correlated response of described purpose composition refers to the correlated response that the character by purpose composition and detection method are arranged.
2. single creature sample multiparameter combined detection system according to claim 1, is characterized in that:
Pipeline of respectively catching in described Acquisition Detection unit is first catch pipeline (21), second catch pipeline (22), the 3rd catch pipeline (23), the 4th catch pipeline (24), the 5th catch pipeline (25) and the 6th and catch pipeline (26) respectively；
Described sample separation unit has sample pool (11), and biological specimen to be checked is infused in described sample pool (11)；
Described sample pool (11) tunnel directly communicates with first passage node (A), and another Lu Jingzhi pipe filter (13) communicates with second channel node (B)；Described first passage node (A) is configured respectively catches the detection sample stop valve and first of pipeline porch in correspondence and catches pipeline (21), second catches pipeline (22) and the 3rd and catch pipeline (23) and communicate；Described second channel node (B) is configured respectively catches the detection sample stop valve and the 4th of pipeline porch in correspondence and catches pipeline (24), the 5th catches pipeline (25) and the 6th and catch pipeline (26) and communicate；
Each reagent and reagent stop valve are set, it is the first reagent (22a) communicated with third channel node (C) by the first reagent stop valve respectively, and is communicated with fourth lane node (D) the second reagent (22b) by the second reagent stop valve；
It is respectively provided with waist valve in the described outlet respectively catching pipeline, each waist valve communicates with waste liquid pool (15) through sewer pipe, arranging Miniature liquid pump (14) in described sewer pipe, by described Miniature liquid pump (14), for sample and reagent, the directed flow in pipeline provides negative pressure driving force.
3. single creature sample multiparameter combined detection system according to claim 2, is characterized in that:
The first detection sample stop valve and first in described first passage node (A) tunnel is caught the entrance of pipeline (21) and is communicated, the entrance that pipeline (22) caught by another road the second detection sample stop valve and second communicates, and another Lu Jing tri-detects sample stop valve and the 3rd entrance catching pipeline (23) communicates；
Described second channel node (B) tunnel the 4th detection sample stop valve and the 4th entrance catching pipeline (24) communicate, another Lu Jing five detects sample stop valve and the 5th entrance catching pipeline (25) communicates, and another Lu Jing six detects sample stop valve and the 6th entrance catching pipeline (26) communicates；
Described third channel node (C) is to communicate through the first reagent stop valve and the 3rd entrance catching pipeline (23), and fourth lane node (D) is to communicate through the second reagent stop valve and the 4th entrance catching pipeline (24).
4. single creature sample multiparameter combined detection system according to claim 1, is characterized in that: described in catch pipeline and refer to that being coated with specific antibody on inner-walls of duct in advance catches pipeline for the cell catching in sample respectively, albumen or free nucleic acid；Described pipeline of catching is also included within vias inner walls grappling in advance and has oligonucleotide probe and catches pipeline for what catch the freestone acid of sample middle reaches.
5. single creature sample multiparameter combined detection system according to claim 2, it is characterized in that: respectively catch the detection sample stop valve of pipeline porch, reagent stop valve by turning off, and turn off the waist valve in the exit respectively catching pipeline, make pipeline to form airtight reaction chamber respectively catching, utilize temperature conditioning unit that each reaction chamber carries out independent temperature to control, respectively constitute cell detection district, Protein Detection district and gene test district.
6. single creature sample multiparameter combined detection system according to claim 1, is characterized in that: arrange temperature control console (31), and be arranged in parallel each road temperature control groove on the top table top of described temperature control console (31)；Described pipeline of catching is pipe fitting, described pipeline one_to_one corresponding of catching is inlaid in described temperature control groove, described temperature control groove is utilized to control to be formed the temperature of the reaction chamber in catching pipeline, at described temperature control console and catch and be covered with heat-conducting glue (32) between pipeline, it is used for bonding temperature control console and catching pipeline.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620153260.7U CN205398640U (en) | 2016-02-29 | 2016-02-29 | Detecting system is united to single biological sample multi -parameter |
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| CN201620153260.7U CN205398640U (en) | 2016-02-29 | 2016-02-29 | Detecting system is united to single biological sample multi -parameter |
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| C14 | Grant of patent or utility model | ||
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| AV01 | Patent right actively abandoned |
Granted publication date: 20160727 Effective date of abandoning: 20171031 |
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| AV01 | Patent right actively abandoned |