CN205368391U - Organ type brain piece culture apparatus - Google Patents
Organ type brain piece culture apparatus Download PDFInfo
- Publication number
- CN205368391U CN205368391U CN201520941933.0U CN201520941933U CN205368391U CN 205368391 U CN205368391 U CN 205368391U CN 201520941933 U CN201520941933 U CN 201520941933U CN 205368391 U CN205368391 U CN 205368391U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- incubator
- brain sheet
- culture apparatus
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an organ type brain piece culture apparatus, organ type brain piece culture apparatus includes incubator and culture dish, the culture dish sets up in the incubator, the bottom plate of culture dish can be dismantled with the lateral wall and be connected, the bottom plate of culture dish is the pellicle. Through the bottom plate of culture dish can be dismantled with the lateral wall and be connected, and will the bottom plate of culture dish sets up to the pellicle, can conveniently take off together bottom the culture dish cultivateing the tissue, avoids cultivateing the tissue and receives the damage.
Description
Technical field
This utility model relates to organ culture's technique with the apparatus field, particularly relates to a kind of organotypic brain sheet culture apparatus.
Background technology
In recent years, after brain sheet carries out the cultivation of long period, tissue is generally tight with the bottom adhesion of culture dish, and subsequent experimental generally requires transfer brain sheet, peel off by force very easily damage brain sheet so that incomplete.Existing transfer method is also only along the profile of brain sheet with stylet, is dismissed together by the thin film of surrounding, is likely to damage tissue while losing time.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of organotypic brain sheet culture apparatus, facilitates cultured tissue to take out in culture dish.
For solving above-mentioned technical problem, embodiment of the present utility model provides a kind of organotypic brain sheet culture apparatus, described organotypic brain sheet culture apparatus includes incubator and culture dish, described culture dish is arranged in described incubator, the base plate of described culture dish and sidewall removably connect, and the base plate of described culture dish is semipermeable membrane.
Preferably, the base plate of described culture dish can bonding or clamping with sidewall.
Preferably, the base plate of described culture dish is provided with outward extending separation protuberance.
Preferably, being provided with support protrusion bottom described incubator, described culture dish is arranged in described support protrusion.
Preferably, described support protrusion is correspondingly arranged along the axis of symmetry bottom incubator.
Preferably, described incubator includes incubator box body and incubator top cover.
Preferably, it is additionally provided with absorbent filter in described incubator.
Having the beneficial effect that of technique scheme of the present utility model:
In such scheme, by removably connecting at the base plate of described culture dish and sidewall, and the base plate of described culture dish is set to semipermeable membrane, it is possible to conveniently cultured tissue taken off together with bottom culture dish, it is to avoid cultured tissue is damaged.
Accompanying drawing explanation
Fig. 1 is the organotypic brain sheet culture apparatus structural perspective of this utility model embodiment.
Fig. 2 is the organotypic brain sheet culture apparatus culture dish structural perspective of this utility model embodiment.
［ main element symbol description ］
Incubator 1；
Culture dish 2；
The base plate 3 of culture dish；
The sidewall 4 of culture dish；
Separate protuberance 5；
Support protrusion 6；
Absorbent filter 7.
Detailed description of the invention
For making the technical problems to be solved in the utility model, technical scheme and advantage clearly, it is described in detail below in conjunction with the accompanying drawings and the specific embodiments.
As illustrated in fig. 1 and 2, a kind of organotypic brain sheet culture apparatus that embodiment of the present utility model provides, described organotypic brain sheet culture apparatus includes incubator 1 and culture dish 2, described culture dish 2 is arranged in described incubator 1, base plate 3 and the sidewall 4 of described culture dish 2 removably connect, and the base plate 3 of described culture dish 2 is semipermeable membrane.
The organotypic brain sheet culture apparatus of this utility model embodiment, by removably connecting at the base plate of described culture dish and sidewall, and the base plate of described culture dish is set to semipermeable membrane, it is possible to conveniently cultured tissue is taken off together with bottom culture dish, it is to avoid cultured tissue is damaged.
Preferably, the base plate 3 of described culture dish can bonding or clamping with sidewall 4.
Preferably, the base plate 3 of described culture dish is provided with outward extending separation protuberance 5.
Preferably, being provided with support protrusion 6 bottom described incubator 1, described culture dish 2 is arranged in described support protrusion 6.
Preferably, described support protrusion is correspondingly arranged along the axis of symmetry bottom incubator.
Preferably, described incubator includes incubator box body and incubator top cover.
Preferably, it is additionally provided with absorbent filter 7 in described incubator 1.
In use,
1. open outer package, after taking out an external member, open incubator top cover, press from both sides out absorbent filter with tweezers.
2. clamp culture dish with tweezers and mention, using pipette, extract culture fluid, instilling the bottom cultivating incubator, then culture dish is put on bottom incubator, rock incubator gently, make semipermeable membrane be cultured liquid wetting completely.
3. it is placed on absorbent filter by freshly prepd brain sheet to exhaust the section liquid of remnants, then brain sheet is transferred on the semipermeable membrane of culture dish.
4. cover incubator top cover, incubator is put in cell culture incubator.
5. it is cultured to required time, during the operation such as the tissue carrying out next step is fixed, dyeing, culture dish can be transferred on microscope slide or corresponding utensil.
6. the base plate of culture dish and slide surface are attached completely, press...withing one's finger and separate protuberance, another hands, near the side separating protuberance, pinches the sidewall of both sides culture dish, slowly pull-up rearwardly and upwardly, makes the sidewall of culture dish and the bottom of culture dish be gradually disengaged.
7. brain sheet and semipermeable membrane are taken off simultaneously, can carry out follow-up test.
The organotypic brain sheet culture apparatus of this utility model embodiment, the independent packaging one to one of culture dish and culture plate, both conveniently took under ensureing aseptic premise, saved again material.
Culture dish is designed as rectangle, and rectangle width, equal to or slightly less than the width of standard glass slide, is beneficial to after semipermeable membrane separates with culture dish and the attaching of microscope slide.
The sidewall wall of culture dish is separable with the semipermeable membrane of base plate, after brain sheet is cultivated, subsequent experimental need to be carried out, carry out tissue to fix, antibody incubation, before the operating process such as SABC, separable culture dish and semipermeable membrane, under the premise not damaging brain sheet, brain sheet is transferred on microscope slide or other experimental apparatus.
With an absorbent filter in each independent packaging, go forward freshly prepd brain sheet is transferred to culture dish film, first clean remnants section liquid with absorbent filter, transfer in culture dish.
The above is preferred implementation of the present utility model; it should be pointed out that, for those skilled in the art, under the premise without departing from principle described in the utility model; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (7)
1. an organotypic brain sheet culture apparatus, it is characterized in that, described organotypic brain sheet culture apparatus includes incubator and culture dish, and described culture dish is arranged in described incubator, the base plate of described culture dish and sidewall removably connect, and the base plate of described culture dish is semipermeable membrane.
2. organotypic brain sheet culture apparatus according to claim 1, it is characterised in that the base plate of described culture dish can bonding or clamping with sidewall.
3. the organotypic brain sheet culture apparatus according to claim 1 or 2 any one, it is characterised in that the base plate of described culture dish is provided with outward extending separation protuberance.
4. organotypic brain sheet culture apparatus according to claim 1, it is characterised in that being provided with support protrusion bottom described incubator, described culture dish is arranged in described support protrusion.
5. organotypic brain sheet culture apparatus according to claim 4, it is characterised in that described support protrusion is correspondingly arranged along the axis of symmetry bottom incubator.
6. the organotypic brain sheet culture apparatus according to claim 4 or 5 any one, it is characterised in that described incubator includes incubator box body and incubator top cover.
7. organotypic brain sheet culture apparatus according to claim 1, it is characterised in that be additionally provided with absorbent filter in described incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520941933.0U CN205368391U (en) | 2015-11-24 | 2015-11-24 | Organ type brain piece culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520941933.0U CN205368391U (en) | 2015-11-24 | 2015-11-24 | Organ type brain piece culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205368391U true CN205368391U (en) | 2016-07-06 |
Family
ID=56261554
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520941933.0U Active CN205368391U (en) | 2015-11-24 | 2015-11-24 | Organ type brain piece culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205368391U (en) |
-
2015
- 2015-11-24 CN CN201520941933.0U patent/CN205368391U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101828910B1 (en) | Cell release method | |
| WO2014017481A1 (en) | Method of regionally selective cell detachment and methods for culturing and subculturing cells using same | |
| Schneider et al. | Differentiating the stem cell pool of human hair follicle outer root sheath into functional melanocytes | |
| CN205368391U (en) | Organ type brain piece culture apparatus | |
| CN206945404U (en) | A kind of Dyeing pipe for being used for not attached cell immunostaining | |
| CN207456902U (en) | A set of cell climbing sheet batch dyeing apparatus | |
| CN212713579U (en) | Cell climbing device | |
| JPH01174373A (en) | Laboratory dish, laboratory dish system, cell culture apparatus using thereof and culturing method | |
| CN205473827U (en) | Cell slide's slide glass support | |
| CN203429184U (en) | Multi-adherent-cell coculture device | |
| CN208586298U (en) | It is a kind of for dye observation Tissue Culture Dish | |
| CN203754728U (en) | Cell culture plate for cell immunofluorescence and immunochemical research | |
| CN216786150U (en) | Cell slide culture plate | |
| CN208414458U (en) | A kind of immunofluorescent staining tissue culture plate | |
| CN205848267U (en) | A kind of culture bottle field planting holder | |
| CN108865737A (en) | One kind splitting formula quantitative visual out and holds criss-cross method surface sampling culture dish | |
| CN108841601A (en) | One kind splitting formula leakproof out and holds surface sampling culture dish | |
| CN108841660A (en) | One kind splitting formula quantitative anti-skid out and visually holds surface sampling culture dish | |
| CN108893279A (en) | One kind splitting formula anti-slip visible surface sampling culture dish out | |
| CN108865785A (en) | One kind splitting the anti-skidding hand-held surface sampling culture dish of formula out | |
| CN108893337A (en) | One kind splitting formula anti-slip visible out and holds criss-cross method surface sampling culture dish | |
| CN108865856A (en) | One kind splitting formula quantitative anti-skid out and holds criss-cross method surface sampling culture dish | |
| CN108893277A (en) | One kind splitting formula leakproof visible surface sampling culture dish out | |
| CN108841598A (en) | One kind splitting the quantitative easily pressure surface sampling culture dish of formula out | |
| CN108865714A (en) | One kind splitting formula leakproof out and visually holds criss-cross method surface sampling culture dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |