CN205347392U - Electronic unicellular control system of micro -nano level based on water pressure - Google Patents
Electronic unicellular control system of micro -nano level based on water pressure Download PDFInfo
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- CN205347392U CN205347392U CN201620020663.4U CN201620020663U CN205347392U CN 205347392 U CN205347392 U CN 205347392U CN 201620020663 U CN201620020663 U CN 201620020663U CN 205347392 U CN205347392 U CN 205347392U
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- linear moving
- micro
- clip holder
- probe
- moving table
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Abstract
The utility model discloses an electronic unicellular control system of micro -nano level based on water pressure. Linear moving platform support frame vertical fixation is on the workstation, and u -shaped frame fixes the bottom surface on the keysets, and linear moving platform installs in linear moving platform support frame side, and the keysets is fixed on linear moving platform's mobile station, be equipped with liquid container in the u -shaped frame, two thread turn of liquid container upper cover connect and connect the two -port valve respectively, and ware one end is held to visiting needle holder to the 3rd thread turn articulate of liquid container lower cover, and the other end is equipped with the probe, visit needle holder and hold the ware card in spy needle holder holds the spring clamp slot of ware anchor clamps to fix at another keysets lower extreme, its upper end is connected to on little behaviour's platform. This system's triaxial precision reaches~7nm, and stroke 20mm is little to the living cell damage, cell control is stable, controls hydraulic linear moving platform precision and is 0.25 mu m, it can be used to unicellular little behaviour's experiment and easily integrates the experimental system based on unicellular little behaviour.
Description
Technical field
This utility model relates to Manipulation of single cells system, especially relates to the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure.
Background technology
Along with the development of bio-physical method in recent years, occur in that a lot of novelty, efficient method, and these emerging systems generally require the submodule utilizing molding and are incorporated into dexterously in new system.In a lot of cell experiments, owing to cell size is less, real-time observation needs based on microscope and CCD.Want to observe unicellular change (such as pattern, fluorescence, transmembrane potential etc.) under various conditions, it is necessary first to will unicellular fix, and requirement can migratory cell as required.Therefore, cell operation system is the basis of a lot of unicellular experimental technique.Seldom there is the system of this type of molding in the market, especially as submodule, it is possible to the cell operation system being integrated into new system simply, easily is more rare.
Apply more method at present and mainly produce suction by hydraulic pressure/baric systerm formation negative pressure, be adsorbed on capillary micro pipette tip by unicellular.First, it is contemplated that a lot of unicellular experiments have the requirement of against shock, experimentation obviously can be produced impact by the use of air pump；Secondly, the precision of the suction of fixing cell also requires that high, and the excessive easy damaging cells of suction, suction is too small, and cell is easily escaped；Again, the small size of cell generally requires high power objective and observes, and the visual field of high power objective is generally only small, is difficult to cell realizes the extremely accurate other micromanipulation of micro/nano level.Accordingly, it would be desirable to the high accuracy cell operation system of a kind of pressure controllable precise based on hydraulic pressure.
Utility model content
This utility model purpose is in that to provide the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure.With high-precision linear mobile platform, hydraulic pressure is accurately controlled, and carry out accurate Three dimensions control with nanoscale micro-behaviour platform to unicellular.
For achieving the above object, the technical solution adopted in the utility model is:
This utility model includes probe, Probe clip holder fixture, Probe clip holder, the second keyset, micro-behaviour's platform, linear moving table, linear moving table bracing frame, liquid container, the first keyset, U-frame frame, three screw thread adaptors, two Rule two-port valves, syringe and three medical silicone tubes；
Linear moving table bracing frame is vertically fixed on workbench, and linear moving table is arranged on linear moving table bracing frame side, and the first keyset is fixed on the mobile platform of linear moving table；U-frame frame bottom surface is fixed on the first keyset；U-frame frame is built with liquid container, liquid container screw rod is fixed in U-frame frame, liquid container upper cover is equipped with first, second screw thread adaptor, first, second screw thread adaptor is connected with one end of respective medical silicone tube respectively, first, the other end of the second medical silicone tube is connected with respective Rule two-port valve, the other end of two Rule two-port valves can connect syringe, liquid container lower cover is equipped with the 3rd screw thread adaptor, 3rd screw thread switching is connected with one end of the 3rd medical silicone tube, the other end of the 3rd medical silicone tube is connected to one end of Probe clip holder, the other end of Probe clip holder is equipped with probe；Probe clip holder is stuck in the spring card slot of Probe clip holder fixture, and Probe clip holder fixture is fixed in the second keyset lower end screw, and the second keyset upper end is connected on micro-behaviour's platform.
Described Probe clip holder fixture model is Narishige Products HIK-3 Probe clip holder fixture.
Described Probe clip holder model is Narishige Products HI-7 Probe clip holder.
Described micro-behaviour's platform model is the three-dimensional micro-behaviour's platform of Sensapex.
Described linear moving table model is the M404 linear moving table of PhysicInstrument Products.
This utility model has the beneficial effect that
Hydraulic pressure, mainly for the Manipulation of single cells problem of level micro-nano in life science, is accurately controlled by this utility model with high-precision linear mobile platform, and carries out accurate Three dimensions control with nanoscale micro-behaviour platform to unicellular, has following advantage concurrently:
1) unicellular easy and simple to handle, spatial control precision is high and scope wide (X, Y, Z tri-axle precision up to ~ 7nm, span of control ~ 20mm), controls process active somatic cell damage is little；
2) cell controls stable, hydraulic pressure pressure controling precision high (precision is 0.25 μm).
3) this system can be widely applied to based in unicellular or the micrurgic complex experiment system of micron particle.
Accompanying drawing explanation
Fig. 1 is system structure schematic diagram of the present utility model.
Fig. 2 is the structure chart of this utility model hydraulic pressure path.
Fig. 3 is the structure chart of this utility model linear moving table bracing frame.
Fig. 4 is the structure chart of this utility model U-frame frame.
Fig. 5 is the structure chart of this utility model the second keyset.
In figure: 1, probe, 2, Probe clip holder fixture, 3, Probe clip holder, 4, the second keyset, 5, micro-behaviour's platform, 6, linear moving table, 7, linear moving table bracing frame, 8, liquid container, 9, the first keyset, 10, U-frame frame, 11, screw thread adaptor, 12, Rule two-port valve, 13, syringe.14, medical silicone tube.
Detailed description of the invention
Below in conjunction with drawings and Examples, the utility model is described in further detail.
As it is shown in figure 1, this utility model includes probe 1, Probe clip holder fixture 2, Probe clip holder 3, the second keyset 4, micro-behaviour's platform 5, linear moving table 6, linear moving table bracing frame 7, liquid container 8, the first keyset 9, U-frame frame 10, three screw thread adaptors 11, two Rule two-port valves 12, syringe 13 and three medical silicone tubes 14.
As shown in Figure 1 and Figure 2, linear moving table bracing frame 7 is vertically fixed on workbench, linear moving table 6 screw is arranged on linear moving table bracing frame 7 side (as shown in Figure 3), and the first keyset 9 is fixed on the mobile platform of linear moving table 6；U-frame frame 10 bottom surface is fixed on the first keyset 9 (as shown in Figure 4)；U-frame frame 10 is built with liquid container 8, liquid container 8 screw rod is fixed in U-frame frame 10, liquid container 8 upper cover is equipped with first, second screw thread adaptor 11, first, second screw thread adaptor 11 is connected with one end of respective medical silicone tube 14 respectively, first, the other end of the second medical silicone tube 14 is connected with respective Rule two-port valve 12, the other end of two Rule two-port valves 12 can connect syringe 13, liquid container 8 lower cover is equipped with the 3rd screw thread adaptor 11, 3rd screw thread adaptor 11 is connected with one end of the 3rd medical silicone tube 14, the other end of the 3rd medical silicone tube 14 is connected to one end of Probe clip holder 3, the other end of Probe clip holder 3 is equipped with probe 1；Probe clip holder 3 is stuck in the spring card slot of Probe clip holder fixture 2, and Probe clip holder fixture 2 is fixed in the second keyset 4 lower end screw, and the second keyset 4 upper end is connected on micro-behaviour's platform 5 (as shown in Figure 5).
Described Probe clip holder fixture 2 model is Narishige Products HIK-3 Probe clip holder fixture.
Described Probe clip holder 3 model is Narishige Products HI-7 Probe clip holder.
Described micro-behaviour's platform 5 model is the micro-behaviour's platform of Sensapex.
Described linear moving table 6 model is M404 linear moving table (the stroke 200m millimeter of PhysicInstrument (PI) Products, minimum step precision 0.25 micron, controller PhysikInstrument (PI) company C884.4D, could support up 4 tunnels, interface: TCP/IP, USB1, RS-232) M404 linear moving table controller C884 connects computer by USB interface, the control software design PIMICROMOVE provided by PI Corp. is controlled, and according to user's needs, can be integrated in other system with different programming language codings.
Operation principle of the present utility model is as follows:
Probe 1 needs bore to carry out opening process according to user, and will fill water in probe 1；Opening Rule two-port valve 12 makes liquid level in liquid container 8 connect with atmospheric pressure, the liquid container 8 filling water can move up and down with mobile of linear moving table 6, and then adjust the relative altitude of the water surface and probe 1 tip in liquid container 8, thereby through linear moving table 6 being moved the control to the most advanced and sophisticated pressure size produced of probe 1 of the accurate control realization of platform height.If liquid level is higher than probe 1 tip in liquid container 8, produce normal pressure, can by most advanced and sophisticated for probe 1 cell blowout；If liquid level is lower than probe 1 tip in liquid container 8, produce negative pressure, most advanced and sophisticated for probe 1 neighbouring cell can be drawn onto probe tip.
, take off fixing for linear moving table bracing frame 7 Probe clip holder 3 simultaneously by Rule two-port valve 12 to water filling in liquid container and raise on the table, utilizing the bubble in law of connected vessels emptying the 3rd medical silicone tube.Take out probe 1, Probe clip holder 3 is placed below liquid level position in liquid container 8 until Probe clip holder 3 tip has continuous drop uniformly to reserve, now empty the bubble in hydraulic path, it is possible to move the control realization accurate control to probe tip pressure of platform height by controlling linear moving table 6.
Need zero-pressure height is corrected before carrying out micrurgy: by mobile of programme-control linear moving table 6 height adjusting hydraulic container 8, observe the movement tendency of suspension cell near probe tip simultaneously, until near probe tip cell not only not close to but also not away from probe tip, the position that now linear moving table 6 moves platform is set to zero pressure height.
When testing, the three-dimensional micro-behaviour's platform 5 of Sensapex is fixed on microscope carrier, adjust the quasi-Jiao's spiral of microscope, target cell is made clearly to occur in the visual field, by controlling the three-dimensional mobile by most advanced and sophisticated for probe 1 centre position, the visual field adjusting focal plane of the three-dimensional micro-behaviour's platform 5 of Sensapex, after finding target cell, probe 1 tip is moved near cell, adjust mobile height of linear moving table 6, cell inspiration probe 1 is most advanced and sophisticated.Continue to adjust mobile height of linear moving table 6, the cell held is adjusted ideal form, experiment can be started.After experiment terminates, adjust mobile height of linear moving table 6, by most advanced and sophisticated for probe 1 cell release, select new target cell and repeatable experiment.
When needs are to when adding water in liquid container 8, two Rule two-port valves 12 are in open mode, connect the other end of one of them Rule two-port valve with syringe 13, can water filling wherein.
When probe 1 tip is blocked by foreign body, one of them Rule two-port valve 12 can cut out, connect another one Rule two-port valve 12 with syringe 13, the air in syringe 13 is pressed into, extra normal pressure is provided in hydraulic pressure system, helps to discharge the foreign body at probe 1 tip.
Experiment closes two Rule two-port valves 12 after all terminating, it is prevented that liquid evaporation and foreign body enter.
Above-mentioned detailed description of the invention is used for illustrating this utility model; rather than this utility model is limited; in spirit of the present utility model and scope of the claims, any amendment that this utility model is made and change, both fall within protection domain of the present utility model.
Claims (5)
1. the electronic unicellular control system of the micro/nano level based on hydraulic pressure, it is characterized in that: include probe (1), Probe clip holder fixture (2), Probe clip holder (3), second keyset (4), micro-behaviour's platform (5), linear moving table (6), linear moving table bracing frame (7), liquid container (8), the first keyset (9), U-frame frame (10), three screw thread adaptors, two Rule two-port valves, syringe (13) and three medical silicone tubes；Linear moving table bracing frame (7) is vertically fixed on workbench, linear moving table (6) is arranged on linear moving table bracing frame (7) side, and the first keyset (9) is fixed on the mobile platform of linear moving table (6)；U-frame frame (10) bottom surface is fixed on the first keyset (9)；U-frame frame (10) is built with liquid container (8), liquid container (8) screw rod is fixed in U-frame frame (10), liquid container (8) upper cover is equipped with first, second screw thread adaptor, first, second screw thread adaptor is connected with one end of respective medical silicone tube respectively, first, the other end of the second medical silicone tube is connected with respective Rule two-port valve, the other end of two Rule two-port valves can connect syringe (13), liquid container (8) lower cover is equipped with the 3rd screw thread adaptor, one end of 3rd screw thread adaptor and the 3rd medical silicone tube connects, the other end of the 3rd medical silicone tube is connected to one end of Probe clip holder (3), the other end of Probe clip holder (3) is equipped with probe (1)；Probe clip holder (3) is stuck in the spring card slot of Probe clip holder fixture (2), Probe clip holder fixture (2) is fixed in the second keyset lower end screw, and the second keyset upper end is connected on micro-behaviour's platform (5).
2. the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure according to claim 1, it is characterised in that: described Probe clip holder fixture (2) model is Narishige Products HIK-3 Probe clip holder fixture.
3. the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure according to claim 1, it is characterised in that: described Probe clip holder (3) model is Narishige Products HI-7 Probe clip holder.
4. the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure according to claim 1, it is characterised in that: described micro-behaviour's platform (5) model is the three-dimensional micro-behaviour's platform of Sensapex.
5. the electronic unicellular control system of a kind of micro/nano level based on hydraulic pressure according to claim 1, it is characterised in that: described linear moving table (6) model is the M404 linear moving table of PhysicInstrument Products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020663.4U CN205347392U (en) | 2016-01-11 | 2016-01-11 | Electronic unicellular control system of micro -nano level based on water pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020663.4U CN205347392U (en) | 2016-01-11 | 2016-01-11 | Electronic unicellular control system of micro -nano level based on water pressure |
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| CN205347392U true CN205347392U (en) | 2016-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620020663.4U Withdrawn - After Issue CN205347392U (en) | 2016-01-11 | 2016-01-11 | Electronic unicellular control system of micro -nano level based on water pressure |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505743A (en) * | 2016-01-11 | 2016-04-20 | 浙江大学 | Micro-nano-scale electric unicell control system based on water pressure |
-
2016
- 2016-01-11 CN CN201620020663.4U patent/CN205347392U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505743A (en) * | 2016-01-11 | 2016-04-20 | 浙江大学 | Micro-nano-scale electric unicell control system based on water pressure |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160629 Effective date of abandoning: 20170714 |