CN210065764U - Novel stem cell extraction device - Google Patents
Novel stem cell extraction device Download PDFInfo
- Publication number
- CN210065764U CN210065764U CN201920686096.XU CN201920686096U CN210065764U CN 210065764 U CN210065764 U CN 210065764U CN 201920686096 U CN201920686096 U CN 201920686096U CN 210065764 U CN210065764 U CN 210065764U
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- stem cell
- extraction device
- fixed
- cell extraction
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Abstract
The utility model discloses a novel stem cell draws device, including base and sliding frame, the upper surface of base is opened there is the rectangle spout, and the joint has the film in the rectangle spout, the upper surface of film is fixed with epidermis body and coverslip respectively, the top of sliding frame is close to the edge and is provided with the mount of L shape structure, and the top of mount is provided with fixed bucket, the circumference inner wall sliding connection of fixed bucket has the piston dish, and the lower surface middle part joint of piston dish has the straw, the top of piston dish is fixed with the piston rod, the circumference outer wall of fixed bucket is close to the bottom and pegs graft and has the intermediate tube, and is close to the top joint on the mount and have the syringe, the upper surface of base is close to the edge and is fixed with the mount pad. The utility model discloses can will treat the stem cell of drawing to separate from the cell crowd when the straw descends to the non-slip groove of separation shovel inner wall can increase the frictional force on and stem cell surface can be easily with great cell centre gripping.
Description
Technical Field
The utility model relates to a cell extraction technical field especially relates to a novel stem cell draws device.
Background
Epidermal stem cells, which are cells having the potential to produce at least one more highly differentiated daughter cell, play an important role in the protection against microbial invasion, ultraviolet radiation, and water loss, regulation of body temperature, and are also one of the components of the immune system.
The skin is a tissue with strong regeneration capacity, the epidermis on the outer layer of the skin continuously renews itself for life, stem cells on the base part of the skin continuously proliferate and differentiate to replace the terminally differentiated cells on the outer layer, so that the tissue structure is renewed, the death and the abscission of the cells on the outer layer and the division of the stem cells on the base layer maintain a certain balance, which is the basic requirement for maintaining the normal tissue structure and the intracellular environment stability. Therefore, understanding how epidermal stem cells proliferate and differentiate and their regulation and control mechanisms are of great significance for promoting complete repair of damaged skin functions and structures.
Most of the existing stem cell extraction devices use a pipette to aspirate epidermal cells under a microscope, but this method has certain requirements on the stability of the manipulation of an operator, so that a stem cell extraction device with good stability is required to obtain epidermal cells to improve the precision of aspiration.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a novel stem cell extraction device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a novel stem cell extraction device, includes base and sliding frame, the upper surface of base is opened there is the rectangle spout, and the joint has the film in the rectangle spout, the upper surface of film is fixed with epidermis body and cover glass respectively, the top of sliding frame is close to the mount that the edge was provided with L shape structure, and the top of mount is provided with fixed bucket, the circumference inner wall sliding connection of fixed bucket has the piston dish, and the lower surface middle part joint of piston dish has the straw, the top of piston dish is fixed with the piston rod, the circumference outer wall of fixed bucket is close to the bottom and pegs graft and has the intervalve, and is close to the top joint on the mount and have the syringe, the upper surface of base is close to the edge and is fixed with the mount pad, and the equal joint in both sides of mount pad has the internal thread pipe, two rotate between the internal thread pipe and.
Preferably, a groove with a trapezoidal cross section is reserved on the lower surface of the base, and rubber anti-slip blocks are clamped on the lower surface of the base close to the four corners.
Preferably, the outer wall of the bottom plate is clamped in the rectangular sliding groove, and the bottom plate and the cover glass are the same in size.
Preferably, the circumference outer wall of straw is provided with two shelves poles near the top, and the circumference outer wall of straw is provided with the through-hole of equidistance distribution near the top that shelves the pole.
Preferably, the circumferential outer wall of the suction pipe is provided with two symmetrical separation shovels close to the bottom end, and the middle parts of the two opposite sides of the separation shovels are provided with anti-skid grooves distributed at equal intervals.
Preferably, the end part, close to the screw rod, of the outer wall of the sliding frame is provided with a movable sleeve ring, and the end part of the screw rod is provided with a raised head matched with the movable sleeve ring.
Preferably, the inner diameter of the middle tube is matched with the outer diameter of the head of the injector, the inner wall of the sliding frame is provided with a fixed support, and the middle of the fixed support is clamped with a guide ring matched with the suction tube.
Compared with the prior art, the utility model provides a novel stem cell extraction device possesses following beneficial effect:
1. through the separation shovel that sets up near both sides in the straw bottom, can separate the stem cell who treats to draw from the cell crowd when the straw descends to the non-slip groove of separation shovel inner wall can increase and can be easily with the surperficial frictional force of stem cell centre gripping of great cell.
2. Through the sliding frame of sliding connection on the base surface that sets up and the lead screw of rotating connection in the loop inside living, can slowly make the sliding frame remove about through the other end that rotates the lead screw to let the bottom of straw just can be to the cell that will draw directly over.
3. Through the through-hole of shelves pole and straw top that sets up to can be with the cell that the straw was inhaled to transfer to fixed bucket in through-hole, the shelves pole can prevent to have the low excessively that the straw that the through-hole section was opened descends and block the opening of middle pipe moreover.
The part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, the utility model discloses simple structure, convenient operation and adjust convenient accuracy height.
Drawings
FIG. 1 is a schematic cross-sectional view of a novel stem cell extraction device according to the present invention;
fig. 2 is a schematic view of a three-dimensional structure of a separation shovel of the novel stem cell extraction device provided by the utility model;
fig. 3 is an enlarged schematic structural view of a position a of the novel stem cell extraction device provided by the present invention.
In the figure: 1-base, 2-rectangular chute, 3-cover glass, 4-separation shovel, 5-bottom sheet, 6-fixed support, 7-sliding frame, 8-suction pipe, 9-fixed barrel, 10-piston rod, 11-piston disc, 12-through hole, 13-stop rod, 14-middle tube, 15-injector, 16-fixed frame, 17-movable sleeve ring, 18-lead screw, 19-internal threaded tube, 20-mounting seat and 21-anti-slip groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1-3, a novel stem cell extraction device comprises a base 1 and a sliding frame 7, wherein a rectangular sliding groove 2 is formed on the upper surface of the base 1, a bottom sheet 5 is clamped in the rectangular sliding groove 2, the upper surface of the bottom sheet 5 is respectively fixed with a surface skin body and a cover glass 3, the top end of the sliding frame 7 close to the edge is provided with an L-shaped fixing frame 16, and the top end of the fixed mount 16 is provided with a fixed barrel 9, the circumferential inner wall of the fixed barrel 9 is connected with a piston disc 11 in a sliding way, a suction pipe 8 is clamped in the middle of the lower surface of the piston disc 11, a piston rod 10 is fixed at the top end of the piston disc 11, a middle pipe 14 is inserted in the circumferential outer wall of the fixed barrel 9 close to the bottom end, an injector 15 is clamped on the fixed frame 16 near the top end, a mounting seat 20 is fixed on the upper surface of the base 1 near the edge, and the both sides of mount pad 20 all the joint have internal thread pipe 19, rotate between two internal thread pipes 19 and be connected with same root lead screw 18.
The utility model discloses in, base 1's lower surface is reserved has the recess that the cross-section is trapezium structure, and base 1's lower surface is close to the equal joint of four corners and has the rubber non slipping spur.
Wherein, the outer wall of the bottom plate 5 is clamped in the rectangular sliding groove 2, and the bottom plate 5 and the cover glass 3 have the same size.
Wherein, the circumference outer wall of the suction pipe 8 is provided with two blocking rods 13 near the top end, and the circumference outer wall of the suction pipe 8 is provided with through holes 12 distributed equidistantly above the blocking rods 13, thereby the cells sucked up by the suction pipe 8 can be transferred to the fixed barrel 9 through the through holes 12, and the blocking rods 13 prevent the opening of the middle pipe 14 from being blocked by the over-low descending of the suction pipe provided with the section of the through holes 12.
Wherein, the circumference outer wall of straw 8 is provided with two symmetrical separation shovels 4 near the bottom, and the middle part of one side that two separation shovels 4 are relative all opens the antiskid groove 21 that the equidistance distributes.
Wherein, the end part of the outer wall of the sliding frame 7 close to the screw rod 18 is provided with a movable sleeve ring 17, and the end part of the screw rod 18 is provided with a raised head matched with the movable sleeve ring 17.
Wherein, the inner diameter of the middle tube 14 is matched with the outer diameter of the head of the injector 15, the inner wall of the sliding frame 7 is provided with a fixed bracket 6, and the middle of the fixed bracket 6 is clamped with a guide ring matched with the suction tube 8.
The working principle is as follows: when the device is used, firstly, the cell epidermis to be sucked is placed between the cover glass 3 and the bottom sheet 5, then the cell sample clamping the bottom sheet 5 is clamped in the rectangular sliding groove 2 above the base 1, then the knob disc at the end part of the screw rod 18 is slowly rotated to slowly push the sliding frame 7 to be right above the cover glass 3, then according to the observation of a microscope, when the suction pipe 8 moves to be right above the stem cells to be extracted, the piston rod 10 is slowly pushed downwards, at the moment, the suction pipe 8 is slowly close to the vicinity of the stem cells to be extracted until the two separation shovels 4 separate the stem cells from cell groups, then the injector 15 is pulled, negative pressure is formed inside the fixing barrel 9, the stem cells can be sucked into the suction pipe, and if the stem cells are required to be continuously sucked, the stem cells can be temporarily stored in the fixing barrel 9 through the through hole 12.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (7)
1. A novel stem cell extraction device comprises a base (1) and a sliding frame (7), and is characterized in that a rectangular sliding groove (2) is formed in the upper surface of the base (1), a bottom sheet (5) is connected in the rectangular sliding groove (2) in a clamping manner, a skin body and a cover glass (3) are respectively fixed on the upper surface of the bottom sheet (5), a fixing frame (16) with an L-shaped structure is arranged at the position, close to the edge, of the top end of the sliding frame (7), a fixing barrel (9) is arranged at the top end of the fixing frame (16), a piston disc (11) is connected to the inner wall of the circumference of the fixing barrel (9) in a sliding manner, a suction pipe (8) is connected to the middle of the lower surface of the piston disc (11) in a clamping manner, a piston rod (10) is fixed at the top end of the piston disc (11), a middle pipe (14) is connected to the outer wall of the circumference of, the upper surface of base (1) is close to the edge and is fixed with mount pad (20), and the equal joint in both sides of mount pad (20) has internal thread pipe (19), two it is connected with same lead screw (18) to rotate between internal thread pipe (19).
2. The novel stem cell extraction device as claimed in claim 1, wherein a groove with a trapezoidal cross section is reserved on the lower surface of the base (1), and rubber anti-slip blocks are clamped on the lower surface of the base (1) near four corners.
3. The novel stem cell extraction device as claimed in claim 1, wherein the outer wall of the bottom sheet (5) is clamped in the rectangular sliding groove (2), and the bottom sheet (5) and the cover glass (3) have the same size.
4. The novel stem cell extraction device as claimed in claim 1, wherein two blocking rods (13) are arranged on the circumferential outer wall of the suction pipe (8) near the top end, and through holes (12) are arranged on the circumferential outer wall of the suction pipe (8) near the blocking rods (13) and are distributed equidistantly.
5. The novel stem cell extraction device as claimed in claim 1, wherein the circumferential outer wall of the suction pipe (8) is provided with two symmetrical separation shovels (4) near the bottom end, and the middle parts of the opposite sides of the two separation shovels (4) are provided with anti-slip grooves (21) distributed at equal intervals.
6. The novel stem cell extraction device as claimed in claim 1, wherein the end of the outer wall of the sliding frame (7) close to the screw rod (18) is provided with a movable sleeve ring (17), and the end of the screw rod (18) is provided with a convex head matched with the movable sleeve ring (17).
7. The novel stem cell extraction device as claimed in claim 1, wherein the inner diameter of the middle tube (14) is matched with the outer diameter of the head of the injector (15), the inner wall of the sliding frame (7) is provided with a fixed support (6), and the middle of the fixed support (6) is clamped with a guide ring matched with the suction tube (8).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201920686096.XU CN210065764U (en) | 2019-05-14 | 2019-05-14 | Novel stem cell extraction device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201920686096.XU CN210065764U (en) | 2019-05-14 | 2019-05-14 | Novel stem cell extraction device |
Publications (1)
Publication Number | Publication Date |
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CN210065764U true CN210065764U (en) | 2020-02-14 |
Family
ID=69451983
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201920686096.XU Expired - Fee Related CN210065764U (en) | 2019-05-14 | 2019-05-14 | Novel stem cell extraction device |
Country Status (1)
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CN (1) | CN210065764U (en) |
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2019
- 2019-05-14 CN CN201920686096.XU patent/CN210065764U/en not_active Expired - Fee Related
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200214 Termination date: 20210514 |
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CF01 | Termination of patent right due to non-payment of annual fee |