CN205263041U - Opposition high performance liquid chromatography post washs and regenerating unit - Google Patents
Opposition high performance liquid chromatography post washs and regenerating unit Download PDFInfo
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- CN205263041U CN205263041U CN201521029780.9U CN201521029780U CN205263041U CN 205263041 U CN205263041 U CN 205263041U CN 201521029780 U CN201521029780 U CN 201521029780U CN 205263041 U CN205263041 U CN 205263041U
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Abstract
The utility model discloses an opposition high performance liquid chromatography post washs and regenerating unit, including the post case that is used for the washing with regeneration opposition high performance liquid chromatography post, the flowing phase storage bottle that is used for splendid attire flowing phase for control flow looks mix proportion the blender, be used for discharging rinse with regeneration opposition high performance liquid chromatography post after the discharge pipe and the control panel of waste liquid, blender and opposition high performance liquid chromatography post fixed mounting are connected with the blender through first pipeline in post incasement, the one end of opposition high performance liquid chromatography post, the other end of opposition high performance liquid chromatography post is connected with the discharge pipe, flowing phase storage bottle is connected with the blender through the second pipeline, control panel sets up the surface at the post case for the temperature, flowing phase mixing ratio, the flowing phase velocity of flow and the during operation that set up the post case are long. Through rinsing the chromatographic column, need not go on on the high performance liquid chromatography appearance, can bigger degree ground accelerate the detection achievement process of high performance liquid chromatography appearance.
Description
Technical field
The utility model relates to technical field of chromatographic analysis, more particularly, relates to a kind of Reversed Phase High Performance cleaning and regeneration device.
Background technology
Up to now, reverse-phase chromatography is most popular technology in high performance liquid chromatography, and it can universal reason be that it can be applicable to most non-polar compounds, much can dissociate and the analysis of ionic compound. Fixing of using in reverse-phase chromatography is naturally occurring hydrophobic compound mutually mostly, therefore, analyte because of they from fixing mutually between interactional different and separated coming, meanwhile, in polarity table, the approaching compound of hydrophobicity also shows similar chromatographic behavior.
Conventionally, in sample substrate, can contain the irrelevant material of some and target analytes. In use, salt, ester class, fatty compound, organic acid, hydrophobin and other biological component are that a part may interactional material occur with chromatographic column. Compared with target analytes, the reserve capability of these materials may be stronger, the compound that reserve capability is weak, as salt, conventionally in dead volume, be rushed out chromatographic column, these interference beyong contemplation can be detected by the detector, show as chromatographic peak bifurcated, baseline fluctuation or even negative peak. If the reserve capability of sample substrate composition in chromatographic column is very strong, and the eluting power of mobile phase does not always all have by force to being enough to these compounds to rush out, so after sample introduction repeatedly, these matrix components that are adsorbed in chromatographic column will be assembled, when abundant, they will fixingly show character mutually as new, and the interaction of analyte and these pollutants can affect separation mechanism, may cause retention time fluctuation and peak to trail. If pollutant is more, post is pressed and may be risen to very high level, even exceedes post and presses limit, also may cause chromatographic column to cave in and form dead volume, and this depends on the position of stopping up formation.
Therefore, chromatographic column after using a period of time needs it clean and regenerate, and to recover the detection effect of its best, this is a comparatively very long process, and need on high performance liquid chromatograph, carry out. This will slow down the process of testing.
Utility model content
The technical problems to be solved in the utility model is, for the above-mentioned defect of prior art, provides a kind of Reversed Phase High Performance cleaning and regeneration device.
The utility model solves the technical scheme that its technical problem adopts: a kind of Reversed Phase High Performance cleaning and regeneration device is provided, comprise for the post case of cleaning and regeneration Reversed Phase High Performance, for the mobile phase storage bottle of splendid attire mobile phase, for controlling the blender of mobile phase mixing match, for discharging discharge pipe and the control panel of the waste liquid after cleaning and regeneration Reversed Phase High Performance; Described blender and Reversed Phase High Performance are fixedly mounted in post case, and one end of Reversed Phase High Performance is connected with blender by the first pipeline; The other end of Reversed Phase High Performance is connected with described discharge pipe; Described mobile phase storage bottle is connected with blender by second pipe; Described control panel is arranged on the outer surface of post case, for temperature, mobile phase mixed proportion, flow rate of mobile phase and the work duration of post case are set.
Reversed Phase High Performance cleaning and regeneration device described in the utility model, wherein, this Reversed Phase High Performance cleaning and regeneration device also comprises the waste liquid bottle for collecting described waste liquid, waste liquid bottle is connected with the liquid outlet of discharge pipe.
Reversed Phase High Performance cleaning and regeneration device described in the utility model, wherein, the quantity of described mobile phase storage bottle is four, described second pipe is tetrad mobile phase capillary, this tetrad mobile phase capillary is made up of four independent capillaries, and every capillary connects a mobile phase storage bottle.
Reversed Phase High Performance cleaning and regeneration device described in the utility model, wherein, described blender comprises quaternary pump and mixing chamber.
Implement Reversed Phase High Performance cleaning and regeneration device of the present utility model, there is following beneficial effect:
(1) by chromatographic column is cleaned, need on high performance liquid chromatograph, not carry out, can accelerate to a greater degree the testing process of high performance liquid chromatograph;
(2) in addition, this chromatographic column cleaning and regeneration device can reduce because cleaning and regeneration produces impact on the high performance liquid chromatograph stability of a system;
(3) Reversed Phase High Performance cleaning and regeneration device of the present utility model is adapted to dissimilar chromatographic column, as regeneration of the regeneration of the cleaning of the residual protein of the cleaning of silica gel bonded chromatographic post, reversed-phase bonded silica post, high molecular polymer chromatographic column, performance liquid chromatographic column taking zirconia as matrix etc., quality assurance is provided while carrying out Detection task for testing staff, and is conducive to raise the efficiency and cost saving.
Brief description of the drawings
Below in conjunction with drawings and Examples, the utility model is described in further detail, in accompanying drawing:
Fig. 1 is the structural representation of the Reversed Phase High Performance cleaning and regeneration device of the utility model preferred embodiment;
Wherein, 1, post case; 2, mobile phase storage bottle; 3, blender; 4, discharge pipe; 5, waste liquid bottle; 6, control panel; 7, the first pipeline; 8, second pipe; A, Reversed Phase High Performance.
Detailed description of the invention
Below, by reference to the accompanying drawings and detailed description of the invention, the utility model is described further:
As shown in Figure 1, a kind of Reversed Phase High Performance cleaning and regeneration device, comprise for the post case 1 of cleaning and regeneration Reversed Phase High Performance, for the mobile phase storage bottle 2 of splendid attire mobile phase, for controlling the blender 3 of mobile phase mixing match, for discharging the discharge pipe 4 of the waste liquid after cleaning and regeneration Reversed Phase High Performance, for collecting waste liquid bottle 5 and the control panel 6 of described waste liquid; Described blender 3 and Reversed Phase High Performance A are fixedly mounted in post case 1, and one end of Reversed Phase High Performance A is connected with blender 3 by the first pipeline 7; The other end of Reversed Phase High Performance A is connected with described discharge pipe 4; Described mobile phase storage bottle 2 is connected with blender 3 by second pipe 8; Waste liquid bottle 5 is connected with the liquid outlet of discharge pipe 4. Described control panel 6 is arranged on the outer surface of post case 1, for temperature, mobile phase mixed proportion, flow rate of mobile phase and the work duration of post case are set.
Wherein, the utility model preferred embodiments is that the quantity of described mobile phase storage bottle is four, certainly, the quantity of mobile phase storage bottle is not limited only to and four, also it can be two, three etc., described second pipe 8 is tetrad mobile phase capillary, and this tetrad mobile phase capillary is made up of four independent capillaries, and every capillary connects a mobile phase storage bottle 2. Described blender 3 comprises quaternary pump and mixing chamber, by quaternary pump, the mobile phase suction in mobile phase storage bottle 2 is mixed to mixing chamber, mixed mobile phase is delivered in chromatographic column and is cleaned by the first pipeline.
The course of work of Reversed Phase High Performance cleaning and regeneration device of the present utility model:
Chromatographic column is arranged in post case, pass through control panel, control post case temperature, mobile phase ratio and flow velocity, work duration etc., open quaternary pump, needed mobile phase is extracted and mixed, enter chromatographic column by the first pipeline, chromatographic column is carried out to cleaning and regeneration repeatedly, finally enter waste liquid bottle by discharge pipe.
To one skilled in the art, can be according to technical scheme described above and design, make other various corresponding changes and deformation, and within these all changes and deformation all should belong to the protection domain of the utility model claim.
Claims (4)
1. a Reversed Phase High Performance cleaning and regeneration device, it is characterized in that, comprise for the post case of cleaning and regeneration Reversed Phase High Performance, for the mobile phase storage bottle of splendid attire mobile phase, for controlling the blender of mobile phase mixing match, for discharging discharge pipe and the control panel of the waste liquid after cleaning and regeneration Reversed Phase High Performance; Described blender and Reversed Phase High Performance are fixedly mounted in post case, and one end of Reversed Phase High Performance is connected with blender by the first pipeline; The other end of Reversed Phase High Performance is connected with described discharge pipe; Described mobile phase storage bottle is connected with blender by second pipe; Described control panel is arranged on the outer surface of post case, for temperature, mobile phase mixed proportion, flow rate of mobile phase and the work duration of post case are set.
2. Reversed Phase High Performance cleaning and regeneration device according to claim 1, it is characterized in that, this Reversed Phase High Performance cleaning and regeneration device also comprises the waste liquid bottle for collecting described waste liquid, and waste liquid bottle is connected with the liquid outlet of discharge pipe.
3. Reversed Phase High Performance cleaning and regeneration device according to claim 1, it is characterized in that, the quantity of described mobile phase storage bottle is four, described second pipe is tetrad mobile phase capillary, this tetrad mobile phase capillary is made up of four independent capillaries, and every capillary connects a mobile phase storage bottle.
4. Reversed Phase High Performance cleaning and regeneration device according to claim 3, is characterized in that, described blender comprises quaternary pump and mixing chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521029780.9U CN205263041U (en) | 2015-12-11 | 2015-12-11 | Opposition high performance liquid chromatography post washs and regenerating unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521029780.9U CN205263041U (en) | 2015-12-11 | 2015-12-11 | Opposition high performance liquid chromatography post washs and regenerating unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205263041U true CN205263041U (en) | 2016-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521029780.9U Active CN205263041U (en) | 2015-12-11 | 2015-12-11 | Opposition high performance liquid chromatography post washs and regenerating unit |
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| CN (1) | CN205263041U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108254478A (en) * | 2018-01-19 | 2018-07-06 | 吉尔生化（上海）有限公司 | A kind of easy refitting regeneration method for preparing chromatographic column |
-
2015
- 2015-12-11 CN CN201521029780.9U patent/CN205263041U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108254478A (en) * | 2018-01-19 | 2018-07-06 | 吉尔生化（上海）有限公司 | A kind of easy refitting regeneration method for preparing chromatographic column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Opposition high performance liquid chromatography post washs and regenerating unit Effective date of registration: 20190606 Granted publication date: 20160525 Pledgee: Panyu Branch of Guangzhou Bank Co., Ltd. Pledgor: Guangzhou Jingcheng Inspection Technology Co.,Ltd. Registration number: 2019440000207 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200622 Granted publication date: 20160525 Pledgee: Panyu Branch of Guangzhou Bank Co.,Ltd. Pledgor: GUANGZHOU JINGCHENG TEST TECHNOLOGY Co.,Ltd. Registration number: 2019440000207 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A cleaning and regeneration device for reversed phase high performance liquid chromatography column Effective date of registration: 20200806 Granted publication date: 20160525 Pledgee: Panyu Branch of Guangzhou Bank Co.,Ltd. Pledgor: GUANGZHOU JINGCHENG TEST TECHNOLOGY Co.,Ltd. Registration number: Y2020440000228 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210118 Granted publication date: 20160525 Pledgee: Panyu Branch of Guangzhou Bank Co.,Ltd. Pledgor: GUANGZHOU JINGCHENG TEST TECHNOLOGY Co.,Ltd. Registration number: Y2020440000228 |
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| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Room 201, No. 6, Yiheng West Road, Dongsha village, Donghuan street, Panyu District, Guangzhou, Guangdong 511402 Patentee after: State inspection and testing holding group Jingcheng Testing Co.,Ltd. Address before: Room 201, No. 6, Yiheng West Road, Dongsha village, Donghuan street, Panyu District, Guangzhou, Guangdong 519175 Patentee before: GUANGZHOU JINGCHENG TEST TECHNOLOGY CO.,LTD. |