CN205258454U - Gas bath capillary type nucleic acid device that amplifys that declines - Google Patents
Gas bath capillary type nucleic acid device that amplifys that declines Download PDFInfo
- Publication number
- CN205258454U CN205258454U CN201521103099.4U CN201521103099U CN205258454U CN 205258454 U CN205258454 U CN 205258454U CN 201521103099 U CN201521103099 U CN 201521103099U CN 205258454 U CN205258454 U CN 205258454U
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- module
- acid amplification
- capillary
- channel module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a gas bath capillary type nucleic acid device that amplifys that declines, which comprises an outer shell, the shell right -hand member sets up the human -computer interaction panel, open on the shell right side has a module to put into the mouth, and the module is put into the mouth and has been placed a plurality of nucleic acid increase passageway module, and a mouthful thermal baffles of the marginal pin joint in bottom is put into to the module, the left end that nucleic acid amplifyd the passageway module is provided with an air current power supply, and vertical the opening of right flank that nucleic acid amplifyd the passageway module has nucleic acid increase passageway, nucleic acid increase passageway module includes upper cover and bottom plate, and the bottom plate middle position is provided with the heat source, and the bottom plate right -hand member is provided with the capillary support, has vertically placed a capillary on the capillary support, the bottom plate is provided with air current processing module lieing in on the position between heat source and the capillary. The utility model discloses a gas bath capillary type nucleic acid device that amplifys that declines, it is simple relatively, the cost is lower, but high efficiency ground filter nucleic acid increase procedure, guarantee the inside temperature homogeneity of device, satisfy nucleic acid increase demand.
Description
Technical field
The utility model relates to technical field of molecular biology, particularly relates to a kind of gas bath capillary typeMiniature nucleic acid amplifier.
Background technology
At present, PCR (PolymeraseChainReaction, PCR) is that one existsThe technology of certain DNA fragmentation of massive duplication in short time. This technology generally comprises sex change, anneals and prolongsStretch three basic steps, along with constantly looping of these three basic steps, specific DNA fragments willCan be Exponential growth, in the short time, can obtain 1,000,000 times to the amount of reactant. Because round pcrHave high specificity, highly sensitive feature, therefore, round pcr has been widely used in turning at presentGene prod detection, viral detection, drug screening, inspection and quarantine, agricultural pest prevent, legal medical expertThe fields such as material evidence, archaeological research and biological anti-terrorism.
For nucleic acid, it is the large biological molecule compound being aggregated into by much nucleotides, for lifeOne of base substance. Nucleic acid is extensively present in all animal and plant cells, microbial body, organismIn nucleic acid normal with protein bound formation nucleoprotein. Different nucleic acid, its chemical composition, nucleotidesPut in order etc. different. According to chemical composition difference, nucleic acid can be divided into ribonucleic acid (being called for short RNA)And DNA (being called for short DNA). Current, conventional nucleic acid amplifier be substantially all towardsTraditional nucleic acid augmentative instrument that laboratory is used, is undertaken by the multiple reaction tubes to same chamber interiorEntirety temperature control, constantly circulation is anti-by sex change, annealing and three steps of extension to impel inner each response sampleShould, and then complete polymerase chain reaction PCR.
According to mode of heating difference, it is gentle that existing nucleic acid amplification system can be divided into alternating temperature metal derby formulaBath formula. Existing alternating temperature metal derby formula and gas bath type system are all that to control reflection by overall temperature control manyThe temperature of individual sample, in use cannot carry out optimization screening to the amplification program of nucleic acid, in systemThe uniformity of the temperature of portion cannot be guaranteed. Therefore, will cause wasting in amplification processThe more time goes to carry out between each reaction tube, easily to occur in amplification program screening and same once amplificationThe result of big-difference.
Therefore,, at present in the urgent need to developing a kind of nucleic acid amplifier, it is relatively simple, costLow, can support the amplification program of nucleic acid is screened, the uniformity of assurance device internal temperature,Guarantee the expanding effect uniformity of multiple samples, meet the demand of nucleic acid amplification.
Utility model content
In view of this, the purpose of this utility model is to provide the miniature nucleic acid amplification of a kind of gas bath capillary typeDevice, it is relatively simple, cost is lower, can sieve the amplification program of nucleic acid quickly and efficientlyChoosing, the uniformity of assurance device internal temperature, is of great practical significance.
For this reason, the utility model provides a kind of gas bath capillary type miniature nucleic acid amplifier, comprisesShell, the right-hand member of described shell is provided with man-machine interaction panel;
The right side of described shell has a module and puts into mouth, and described module is put into mouth and is placed with multipleNucleic acid amplification channel module, the bottom margin that described module is put into mouth is articulated with a thermal baffle;
The left end of described nucleic acid amplification channel module is provided with an air flow power source, described nucleic acid amplificationThe right flank of channel module longitudinally has a nucleic acid amplification passage;
Described nucleic acid amplification channel module comprises upper cover and base plate, and described upper cover is fixedly connected with base plate;
The middle position of described base plate is provided with thermal source, and the right-hand member of described base plate is provided with capillary tube holder,On described capillary tube holder, be longitudinally placed with a capillary;
Described base plate is provided with air-flow processing module being positioned on thermal source and intercapillary position.
Wherein, described air flow power source is axial flow blower.
Wherein, described thermal source is that iron irons the heating wire that aluminium is made.
Wherein, described air-flow processing module is foam copper.
Wherein, described air-flow processing module is the foam copper that porosity is greater than 85%.
Wherein, the longitudinal section of each described nucleic acid amplification channel module is that depth-width ratio is the rectangle of 2:1;
Front end the right of described shell is provided with a total air outlet, described nucleic acid amplification channel moduleRight flank edge has air outlet, and described air outlet is connected with described total air outlet.
Wherein, the both sides, front and back of described man-machine interaction panel are respectively arranged with usb data mouth and device is openedClose 3
Described capillary tube holder is also being provided with temperature sensor near described position capillaceous.
Wherein, described temperature sensor is thermocouple or thermistor.
Wherein, described nucleic acid amplification channel module is to adopt polyimides or polytetrafluoroethylene (PTFE) to makeModule; The inwall of described nucleic acid amplification channel module posts insulation thermal isolation film.
Wherein, described insulation thermal isolation film be adopt that ceramic fibre or nanometer micropore heat-barrier material make absolutelyEdge thermal isolation film.
The technical scheme being provided from above the utility model, compared with prior art, this practicalityNovelly provide a kind of gas bath capillary type miniature nucleic acid amplifier, it is relatively simple, cost is lower,Can screen the amplification program of nucleic acid quickly and efficiently, the uniformity of assurance device internal temperature,The demand that meets nucleic acid amplification, is of great practical significance.
Brief description of the drawings
The entirety of the miniature nucleic acid amplifier of a kind of gas bath capillary type that Fig. 1 provides for the utility modelStructural representation;
Fig. 2 is the structural representation of the nucleic acid amplification passage in Fig. 1;
Fig. 3 is the inner member structural representation of nucleic acid amplification passage shown in Fig. 2;
Fig. 4 obtains for the miniature nucleic acid amplifier of a kind of gas bath capillary type that utilizes the utility model and provideA kind of embodiment of amplified production electrophoresis comparison diagram obtaining of taking pictures;
In figure, 1 is shell, and 2 is man-machine interaction panel, and 3 is device switch, and 4 is usb data mouth,5 is thermal baffle, and 6 is nucleic acid amplification channel module, and 7 is axial flow blower, and 8 is air outlet, and 9 areBase plate, 10 is heating wire, and 11 is foam copper, and 12 is thermocouple, and 13 is capillary tube holder, 14 areCapillary, 20 is total air outlet, 21 put into mouth for module, 60 is upper cover, 61 for nucleic acid amplification logicalRoad.
Detailed description of the invention
In order to make those skilled in the art person understand better the utility model scheme, below in conjunction with accompanying drawingThe utility model is described in further detail with embodiment.
Referring to Fig. 1, the utility model provides a kind of gas bath capillary type miniature nucleic acid amplifier,Comprise the shell 1 of bottom hollow, the right-hand member upper angled of described shell 1 is provided with man-machine interaction panel 2;
The both sides, front and back of described man-machine interaction panel 2 are respectively arranged with usb data mouth 4 and device switch3。
Front end the right of described shell 1 is provided with a total air outlet 20, the right side opening of described shell 1Have a module to put into mouth 21, described module is put into mouth 21 and is placed with four nucleic acid amplification passage mouldsPiece 6, the bottom margin that described module is put into mouth 21 is articulated with a thermal baffle 5.
Referring to Fig. 2, the longitudinal section of each described nucleic acid amplification channel module 6 is rectangle, described nucleic acidAmplification channel module 6 comprises upper cover 60 and base plate 9, and described upper cover 60 is fixedly connected with base plate 9.
The left end of described nucleic acid amplification channel module 6 is provided with an axial flow blower 7, and described nucleic acid expandsThe right flank that increases channel module 6 longitudinally has a nucleic acid amplification passage 61, described nucleic acid amplification passageThe right flank edge of module 6 has air outlet 8, and described air outlet 8 is connected with described total air outlet 20Logical.
In the utility model, in specific implementation, it should be noted that described nucleic acid amplification passage mouldPiece 6 is preferably the heat-resisting channel module of insulation, is used to nucleic acid amplification that the reacting environment of sealing is provided.
In the utility model, described nucleic acid amplification channel module 6 be preferably longitudinal section be rectangle,The module of hollow, the height of described nucleic acid amplification channel module 6 and width are than being 2:1. Longitudinal section isThe design of rectangle, contributes to reduce the aerodynamic energy loss of this channel module inside, ensures entirety simultaneouslyThe stability of structure and compactedness.
For the utility model, in specific implementation, the sex change phase temperature during due to nucleic acid amplification is higher,Therefore, nucleic acid amplification channel module 6 of the present utility model need to have stronger heat resistance. Concrete realNow go up, described nucleic acid amplification channel module 6 is preferably resistance to height such as adopting polyimides, polytetrafluoroethylene (PTFE)The module that temperature plastics are made.
For the utility model, in specific implementation, the inwall of described nucleic acid amplification channel module 6 postsInsulation thermal isolation film. Described insulation thermal isolation film is close to the inwall of nucleic acid amplification channel module 6, is mainlyWhile occurring to flow for reducing the hot-air of nucleic acid amplification channel module inside in the loss of wall heat,Therefore, need lower thermal conductivity factor. Described insulation thermal isolation film is preferably and adopts ceramic fibre or nanometerThe insulation thermal isolation film that micropore heat-barrier material is made.
Referring to Fig. 3, the middle position of described base plate 9 is also provided with heating wire 10 in the lump, described base plate9 right-hand member is provided with capillary tube holder 13, is longitudinally placed with a capillary on described capillary tube holder 13Pipe 14, described capillary tube holder 13 is also provided with T-shaped heat in the position near described capillary 14Galvanic couple 12;
Described base plate 9 is also provided with foam on the position between heating wire 10 and capillary 14Copper 11.
In the utility model, described nucleic acid amplifier also comprises operation control module, with man-machine friendshipPanel 2 is connected mutually, for the operational parameter control of inputting by man-machine interaction panel 2 according to user(comprising proliferation time, amplification temperature and circulation), the power of axial flow blower 7 and resistance wire is carried outControl, to realize user's demand.
In the utility model, in specific implementation, described operation control module is preferably Single-chip ControllingDevice, for the gentle flowing power source of the thermal source to nucleic acid amplification channel module 6 inside (as heating wire) (asAxial flow blower 7) carry out power control.
In the utility model, in specific implementation, it is 30ul's that described capillary 14 is preferably volumeQuartz capillary. Described capillary 14 is nucleic acid samples, buffer solution and the templates etc. for holding reactionThe container of material, for the amplification of reaction nucleic acid samples provides place, its main feature is that thermal conductivity is good.Described capillary tube holder 13 is for placing capillary 14, and in use, described capillary tube holder 13 is insertedEnter to nucleic acid amplification passage 61, thereby ensure that capillary is positioned at the fixed bit of nucleic acid amplification passage 61Put.
In the utility model, described thermocouple 12, as a kind of temperature sensor, is for feeding back coreThe element of acid amplification channel module 6 internal temperatures, is placed near of capillary 14. In specific implementation,It can also be the thermistor that volume is little.
For the utility model, it should be noted that, in order to carry out nucleic acid amplification, first, described axleStream motor 7 is blown into outside cold air in described nucleic acid amplification channel module 6, cold air processWhen heating wire 10, heating wire 10 heats cold air, and heat convection occurs, and makes cold airBecome high temperature air, then, high temperature air flows through foam copper 11, due to foam copper 11 with hot-airIn convective exchange process, its internal temperature reaches unanimity, and therefore can effectively reduce Air Temperature Difference, improvesThe temperature homogeneity of the air flowing out from foam copper 11, makes flow stability and the thermal dispersion of hot-airProperty can improve. There is convection current in the hot-air then, flowing out from foam copper 11 and capillary 14Heat exchange. Therefore, for the utility model, can be by controlling heating wire 10 and aerofoil fan 7Power, changes the hot air temperature of whole nucleic acid amplification channel module inside, and then changes capillaryThe temperature of the 14 inner response samples of placing, thus response sample is completed to sex change, annealing and extension threeIndividual basic step, finally impels polymerase chain reaction PCR to complete.
In specific implementation, the nucleic acid amplifier providing for the utility model, by outside cold airThe parts that are blown in described nucleic acid amplification channel module 6 are not limited to the axial flow blower 7 shown in Fig. 3,Can also be other air flow power source, as long as described in can effectively outside cold air being blown intoIn nucleic acid amplification channel module 6.
In the utility model, in specific implementation, that described heating wire 10 is preferably is common, price is lowHonest and clean iron irons aluminium heating wire, ensures low production cost and the maintenanceability of the utility model device.
In specific implementation, the nucleic acid amplifier providing for the utility model, adds cold airHeat is not limited to the heating wire 10 shown in Fig. 3, can also be other thermals source that can heat, as long as canEffectively cold air is heated.
In the utility model, it should be noted that, foam copper 11 is as a kind of air-flow processing module,Its effect is that improvement adds flow behavior and the heat transfer characteristic of hot-air in the time that channel interior flows. ToolBody is realized upper, and described foam copper 11 can replace with any one and the indulging of nucleic acid amplification channel module 6The air-flow processing module that the high thermal conductivity coefficient material that area of section is equal forms;
Described air-flow processing module can be pumiceous texture body, is preferably the bubble that porosity is greater than 85%Foam copper. When air is when the foam copper 11, because the thermal resistance of foam copper 11 is far smaller than with airSurface heat exchanging thermal resistance, foam copper 11 with hot-air generation Convective Heat Transfer in its internal temperature be tending towardsUnanimously, therefore, can effectively reduce the temperature difference of air, improve the temperature that flows out air from foam copper 11Uniformity. In addition, foam copper can also reduce in the heat-resisting nucleic acid amplification channel module 6 of whole insulationThe temperature of wall, reduce in the time of Air Flow and the exchange heat of wall, reduces whole diabatic processUnnecessary heat waste.
For the utility model, in practical operation, the sample of nucleic acid of experiment use is placed in to independently coreIn acid amplification channel module 6, the thermal baffle 5 that closes, by human-computer interaction interface 2 control increase intoOK. Shown in Fig. 1, one has the miniature gas bath type nucleic acid amplifier of four-way, therefore, and Ke YiOnce in experiment, move four kinds of different amplification programs, reduce whole experimentation required time.
In specific implementation, according to user's needs, the quantity of described nucleic acid amplification channel module 6 is not limitIn four, can also be any number of.
Below in conjunction with concrete implement from, the gas bath capillary type Micro Core that the utility model provides is describedActual effect of acid amplification device.
Utilize the four-way nucleic acid amplifier shown in Fig. 1, to bacillus subtilis, DNA increases,By pcr amplification product electrophoresis (100V, 30min) on 1.5% Ago-Gel, after electrophoresisPcr amplification product uses ethidium bromide staining to observe under uviol lamp.
Whole implementing procedure comprises the following steps:
The first step: nucleic acid extraction. The bacillus subtilis suspended liquid that is 103cfu/mL from concentration, get1600 μ L inject respectively the standard centrifuge tube of 800mL to 2 1.5mL; Utilize cell crushing instrumentCarry out clasmatosis and extract nucleic acid.
Second step: nucleic acid amplification. First, according to bacillus subtilis rrnE gene order design primerP1 and P2, extension increasing sequence length is 494bp. Then, by the DNA profiling liquid 10 μ L of purifying,The mixing dNTP4 μ L of 1pmol/ μ L, the each 1 μ L of the primer P1 of 200pmol and P2,10 × Buffer3 μ L, Taq polymerase 1 μ L also adds aseptic deionized water and is supplemented to 25 μ L and is placed in capillary 14. By hairTubule 14 is placed in capillary tube holder 13, and capillary tube holder is inserted independently in nucleic acid amplification passage 61.
Then, the thermal baffle 5 that closes, arranges the controlled condition of PCR, tool at man-machine interaction panel 2Body is: 94 DEG C of sex change 30s, and 56 DEG C of annealing 30s, 72 DEG C are extended 30s, move 35 circulations.
The 3rd step: PCR product detects. By the amplified production in the present embodiment at 1.5% fine jadeElectrophoresis on lipolysaccharide gel, it is 100V that electrophoretic voltage is set, the time is 30min. PCR after electrophoresisAmplified production uses the ethidium bromide (EB) of 0.5 μ g/mL to dye, and under uviol lamp, observes.
As shown in Figure 4, bp represents the base number of DNA fragmentation, and 1bp refers to a base-pair, and M isDNA molecular amount mark (Marker), C1 is for being Standard PC R instrument blank, C2 is Standard PC RInstrument amplification, C3 is the blank of independent nucleic acid amplification channel module in the utility model, C4For the product of the utility model independence nucleic acid amplification channel module. As shown in Figure 4, blankGroup does not all occur that the relative shinny band of 500bp left and right has appearred in band, C2 with C4. ThusIn visible the present embodiment, independent nucleic acid amplification channel module, can complete bacillus subtilis DNA'sAmplification.
It should be noted that, agarose electrophoresis is for expanding effect is verified, amplification is finishedProduct is placed in Ago-Gel, voltage 100V electrophoresis time 30min. After electrophoresis finishes by agarSugar gel is placed in ultraviolet transilluminator quartz glass platform and detects. Occur relatively shinny in 500bp left and rightBand, known the utility model can increase to bacillus subtilis DNA, can meetAmplification requirement.
Compared with existing nucleic acid amplifier, based on technique scheme, the utility model providesThe miniature nucleic acid amplifier of gas bath capillary type has the following advantages:
1, reaction fast. The hollow that the longitudinal section of installing inner nucleic acid amplification channel module and being is rectangleModule, hot-air kinetic energy rejection in flow process is little, convection effects in the time carrying out heat exchange with capillaryStronger, therefore, in amplification procedure, warming and cooling rate is fast.
2, simple in structure, cost is low. The miniature nucleic acid amplification of gas bath capillary type that the utility model providesDevice does not use centrifugal mixer blower fan, but the longitudinal section area of employing and nucleic acid amplification channel module 6Equal foam copper carrys out even air themperature, has greatly dwindled the volume of nucleic acid amplification channel module 6,Reduce manufacturing cost.
3, support amplification program screening. In the utility model device, each nucleic acid amplification passage canControl by man-machine interaction panel, realize independent operating, because its volume is little, compact conformation canAccording to using different demand integrations, therefore can move different amplification programs at different passages and screen.
In sum, compared with prior art, a kind of gas bath capillary type that the utility model providesMiniature nucleic acid amplifier, it is relatively simple, cost is lower, the expansion to nucleic acid quickly and efficientlyIncreasing program is screened, and the uniformity of assurance device internal temperature meets the demand of nucleic acid amplification, toolThere is great production practices meaning.
The above is only preferred embodiment of the present utility model, it should be pointed out that for this technology and leadsThe those of ordinary skill in territory, not departing under the prerequisite of the utility model principle, can also makeSome improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (10)
1. the miniature nucleic acid amplifier of gas bath capillary type, is characterized in that, comprises shell (1), and the right-hand member of described shell (1) is provided with man-machine interaction panel (2);
The right side of described shell (1) has a module and puts into mouthful (21), described module is put into mouthful (21) and is placed with multiple nucleic acid amplification channel modules (6), and the bottom margin that described module is put into mouthful (21) is articulated with a thermal baffle (5);
The left end of described nucleic acid amplification channel module (6) is provided with an air flow power source, and the right flank of described nucleic acid amplification channel module (6) longitudinally has a nucleic acid amplification passage (61);
Described nucleic acid amplification channel module (6) comprises upper cover (60) and base plate (9), and described upper cover (60) is fixedly connected with base plate (9);
The middle position of described base plate (9) is provided with thermal source, the right-hand member of described base plate (9) is provided with capillary tube holder (13), is longitudinally placed with a capillary (14) on described capillary tube holder (13);
Described base plate (9) is provided with air-flow processing module on the position being positioned between thermal source and capillary (14).
2. device as claimed in claim 1, is characterized in that, described air flow power source is axial flow blower.
3. device as claimed in claim 1, is characterized in that, described thermal source is that iron irons the heating wire that aluminium is made.
4. device as claimed in claim 1, is characterized in that, described air-flow processing module is foam copper.
5. device as claimed in claim 4, is characterized in that, described air-flow processing module is the foam copper that porosity is greater than 85%.
6. device as claimed in claim 1, is characterized in that, the longitudinal section of each described nucleic acid amplification channel module (6) is that depth-width ratio is the rectangle of 2:1;
Front end the right of described shell (1) is provided with a total air outlet (20), the right flank edge of described nucleic acid amplification channel module (6) has air outlet (8), and described air outlet (8) is connected with described total air outlet (20).
7. the device as described in any one in claim 1 to 6, is characterized in that, the both sides, front and back of described man-machine interaction panel (2) are respectively arranged with usb data mouth (4) and device switch (3);
Described capillary tube holder (13) is also provided with temperature sensor in the position near described capillary (14).
8. device as claimed in claim 7, is characterized in that, described temperature sensor is thermocouple or thermistor.
9. device as claimed in claim 1, is characterized in that, the module of described nucleic acid amplification channel module (6) for adopting polyimides or polytetrafluoroethylene (PTFE) to make; The inwall of described nucleic acid amplification channel module (6) posts insulation thermal isolation film.
10. device as claimed in claim 9, is characterized in that, described insulation thermal isolation film is the insulation thermal isolation film that adopts ceramic fibre or nanometer micropore heat-barrier material to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521103099.4U CN205258454U (en) | 2015-12-24 | 2015-12-24 | Gas bath capillary type nucleic acid device that amplifys that declines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521103099.4U CN205258454U (en) | 2015-12-24 | 2015-12-24 | Gas bath capillary type nucleic acid device that amplifys that declines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205258454U true CN205258454U (en) | 2016-05-25 |
Family
ID=55999992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521103099.4U Active CN205258454U (en) | 2015-12-24 | 2015-12-24 | Gas bath capillary type nucleic acid device that amplifys that declines |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205258454U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385568A (en) * | 2015-12-24 | 2016-03-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Gas bath capillary tube type micro nucleic acid amplification device |
| CN106467891A (en) * | 2016-09-22 | 2017-03-01 | 苏州百源基因技术有限公司 | A kind of gas supply device being applied to instrument for extracting nucleic acid and instrument for extracting nucleic acid |
-
2015
- 2015-12-24 CN CN201521103099.4U patent/CN205258454U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385568A (en) * | 2015-12-24 | 2016-03-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Gas bath capillary tube type micro nucleic acid amplification device |
| CN105385568B (en) * | 2015-12-24 | 2018-01-16 | 中国人民解放军军事医学科学院卫生装备研究所 | A kind of miniature nucleic acid amplifier of gas bath capillary type |
| CN106467891A (en) * | 2016-09-22 | 2017-03-01 | 苏州百源基因技术有限公司 | A kind of gas supply device being applied to instrument for extracting nucleic acid and instrument for extracting nucleic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wong et al. | A rapid and low-cost PCR thermal cycler for low resource settings | |
| Oda et al. | Infrared-mediated thermocycling for ultrafast polymerase chain reaction amplification of DNA | |
| Qiu et al. | Instrument-free point-of-care molecular diagnosis of H1N1 based on microfluidic convective PCR | |
| CN205258454U (en) | Gas bath capillary type nucleic acid device that amplifys that declines | |
| CN105779285B (en) | Box type testing device and through type detection equipment | |
| CN103103118A (en) | Nucleic acid amplification and detection reaction tube | |
| CN108636471A (en) | A kind of nucleic acid amplifier and its application | |
| CN107051598A (en) | PCR micro-fluidic chips and its preparation and application method and PCR equipment | |
| CN107129933A (en) | One kind is based on electrically driven (operated) digital microcurrent-controlled pcr chip device | |
| Podwin et al. | Study of the behavior of Euglena viridis, Euglena gracilis and Lepadella patella cultured in all-glass microaquarium | |
| CN105385568B (en) | A kind of miniature nucleic acid amplifier of gas bath capillary type | |
| CN206232730U (en) | The module thermal balance device of PCR gene amplification instrument | |
| CN205241688U (en) | A simple and easy incubator of water bath formula for single culture dish | |
| CN105087517B (en) | A method of recombination multienzyme complex and the seamless clone of external homologous recombination | |
| CN210656874U (en) | Fluorescent quantitative pcr instrument | |
| CN210923478U (en) | Real-time fluorescent quantitative PCR instrument for on-site rapid detection | |
| WO2023197588A1 (en) | Rapid photoheating pcr apparatus using small-size micro-tube as container and method therefor | |
| WO2018028447A1 (en) | Apparatus, method, and system for performing chemical reaction | |
| CN107190002B (en) | Portable pathogenic bacteria nucleic acid-extracting apparatus and detection method | |
| CN103966333A (en) | Biogen high-throughput qualitative detection method for multiple microdroplet polymerase chain reactions | |
| Fernandes et al. | Caught in-between: system for in-flow inactivation of enzymes as an intermediary step in “plug-and-play” microfluidic platforms | |
| CN103282496B (en) | Method for setting temperature of polymerase chain reaction and instrument thereof | |
| CN207365591U (en) | A kind of Biochemistry Experiment heating drying oven | |
| CN204478473U (en) | Direct-reading spectrometer vacuum chamber constant temperature heating device | |
| CN207845625U (en) | A kind of big reaction volume card form PCR amplification device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |