CN205139016U - Aflatoxin B1's detector in on -line measuring maize - Google Patents

Aflatoxin B1's detector in on -line measuring maize Download PDF

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Publication number
CN205139016U
CN205139016U CN201520922962.2U CN201520922962U CN205139016U CN 205139016 U CN205139016 U CN 205139016U CN 201520922962 U CN201520922962 U CN 201520922962U CN 205139016 U CN205139016 U CN 205139016U
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China
Prior art keywords
fluorescent tube
aflatoxin
ccd
detector
maize
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CN201520922962.2U
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Chinese (zh)
Inventor
赵春城
许正建
陈正行
李永富
丁霜霜
李俊丽
罗小虎
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100 Olson Jiangsu Food Safety Technology Co Ltd
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WUXI BAIAOSEN TECHNOLOGY Co Ltd
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Priority to CN201520922962.2U priority Critical patent/CN205139016U/en
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Abstract

The utility model discloses an aflatoxin B1's detector in on -line measuring maize, this detector include CCD, fluorescent tube, transmission band and equipment shell, the transmission band runs through the equipment shell, and the equipment shell above the transmission band is the can, CCD, fluorescent tube are installed on the inside top of equipment shell, and CCD is located equipment shell top center, and the even equally spaced distribution of fluorescent tube is around CCD. The maize sample that contains aflatoxin B1 of different concentration that will be known, through the detector, the positive correlation that reachs aflatoxin B1 concentration and fluorescence value concerns. The aflatoxin B1's of maize concentration before the digital image signal and the positive correlation relation through aflatoxin B1 concentration and fluorescence value that record the maize that awaits measuring calculate. The utility model discloses the direct aflatoxin B1 who detects different maizes surface in succession, greatly reduced the detection cost, improve detection speed.

Description

AFB in a kind of on-line checkingi corn 1detector
Technical field
The utility model relates to microorganism detection field, specifically AFB in a kind of on-line checkingi corn 1detector.
Background technology
AFB 1be the secondary metabolite that Aspergillus flavus and aspergillus parasiticus bacterium etc. produce at occurring in nature, its toxicity is large, and carcinogenicity is strong, and physicochemical property is relatively stable, is one of most extensive chemical carcinogen found at present.Aflatoxin B1 shifts between biosome by food chain, not only affects production and the processing of various agricultural product, affects output and the benefit of agricultural product, and serious have impact on the healthy of people.
Detect AFB 1method mainly contain thin-layered chromatography, high performance liquid chromatography, euzymelinked immunosorbent assay (ELISA) etc.But thin-layered chromatography complex operation, consuming time, the impact that is subject to each factor in analytic process, the accuracy of method is very poor; High performance liquid chromatography can detect multiple toxin simultaneously, and sensitivity is higher, but requires that sample purity is high, expensive equipment, is difficult to popularize; There is the shortcoming such as false positive, false negative in euzymelinked immunosorbent assay (ELISA).
Utilize AFB 1the characteristic of natural fluoresence can be sent under the irradiation of ultraviolet light, excite the fluorescence of generation to detect to it.It is fast that this detection method has speed, and can the feature of accurate quantitative analysis.Fluorescence detector is used to detect the AFB of ultramicron 1, be widely used for the highly sensitive detection method as aflatoxin.But, adopt Fluorometric assay usually to need and the method coupling such as high performance liquid chromatography, pre-treatment isolation technics.Immune affinity column method, often as the earlier stage processing method of the method, this adds increased testing cost and experimental procedure.
Summary of the invention
The purpose of this utility model is that providing a kind of reduces AFB in the on-line checkingi corn of testing cost, raising detection speed 1detector, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the utility model provides following technical scheme:
AFB in a kind of on-line checkingi corn 1detector, comprise CCD, fluorescent tube, transport tape and device housings; Transport tape runs through device housings, and the device housings above transport tape is can; CCD, fluorescent tube are arranged on the top of device housings inside, and CCD is positioned at device housings top center, fluorescent tube comprises the first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the 4th fluorescent tube, first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the size of the 4th fluorescent tube, model are identical, and are distributed in the surrounding of CCD uniformly and equidistantly.
As further program of the utility model: CCD is also connected with computer.
As further program of the utility model: fluorescent tube adopts wavelength to be the uviol lamp of 365nm.
Compared with prior art, the beneficial effects of the utility model are:
AFB in on-line checkingi corn in the utility model 1detector belong to fluorescence detection, it is the AFB of direct-detection sample surfaces 1, and do not need to carry out sample the steps such as pre-treatment, greatly reduce testing cost, improve detection speed, the utility model continuously can detect the AFB in different corn simultaneously 1concentration, and do not affect other samples.
Accompanying drawing explanation
Fig. 1 is AFB in on-line checkingi corn 1the structural representation of detector;
In figure: 1-first fluorescent tube, 2-second fluorescent tube, 3-CCD, 4-the 3rd fluorescent tube, 5-the 4th fluorescent tube, 6-transport tape, 7-device housings.
Embodiment
Below in conjunction with the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Embodiment 1
Refer to Fig. 1, in the utility model embodiment, AFB in a kind of on-line checkingi corn 1detector, comprise CCD3, fluorescent tube, transport tape 6 and device housings 7.Transport tape 6 runs through device housings 7, and the device housings 7 above transport tape 6 is can.CCD3, fluorescent tube are arranged on the top of device housings 7 inside, and CCD3 is positioned at device housings 7 top center, fluorescent tube comprises the first fluorescent tube 1, second fluorescent tube 2, the 3rd fluorescent tube 4, the 4th fluorescent tube 5, first fluorescent tube 1, second fluorescent tube 2, the 3rd fluorescent tube 4, the 4th fluorescent tube 5 size, model are identical, and are distributed in the surrounding of CCD3 uniformly and equidistantly.Fluorescent tube adopts wavelength to be the uviol lamp of 365nm.CCD3 is also connected with computer.
Above-mentioned detector is used for AFB in on-line checkingi corn 1detection method, concrete steps are as described below:
1. transport tape 6 drives corn to be measured average rate from device housings 7 to flow through, and has AFB in corn 1part can send blue-green fluorescent under ultra violet lamp, the power (light intensity) of fluorescence has reacted the number of aflatoxin B1 concentration, light intensity and concentration correlation.
2. by the corn sample of variable concentrations, gather relevant data image signal by CCD3, computer reads fluorescent value in analysing digital image signal and and AFB 1concentration sets up linear relationship curve.
3. on high speed high definition CCD3 continuous acquisition transport tape 6 corn data image signal and transfer on computer, pass through AFB 1the positive correlation of concentration and fluorescent value calculates the AFB of current corn 1concentration and compare with pre-set threshold value to identify whether current corn exceeds standard.
4. the concentration data of current corn is communicatively real-time transmitted to host computer, and the information that will exceed standard is transferred to digital switch quantity form the sample processing device that exceeds standard.
CCD in the utility model, (Charge-coupledDevice, charge coupled cell), can be called ccd image sensor, also be image controller.CCD is a kind of semiconductor devices, optical image can be converted into digital signal.The small photoactive substance of the upper implantation of CCD is called pixel (Pixel).The pixel count that one piece of CCD comprises is more, and its screen resolution provided is also higher.The effect of CCD is just as film, but it is that light signal is converted to charge signal.CCD there is the photodiode of many marshallings, can light be responded to, and light signal is transformed into electric signal, convert data image signal to through external sampling amplification and analog to digital conversion circuit.
To those skilled in the art, obvious the utility model is not limited to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present utility model or essential characteristic, can realize the utility model in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the utility model.
In addition, be to be understood that, although this instructions is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of instructions is only for clarity sake, those skilled in the art should by instructions integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.

Claims (3)

1. AFB in an on-line checkingi corn 1detector, it is characterized in that, comprise CCD, fluorescent tube, transport tape and device housings; Transport tape runs through device housings, and the device housings above transport tape is can; CCD, fluorescent tube are arranged on the top of device housings inside, and CCD is positioned at device housings top center, fluorescent tube comprises the first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the 4th fluorescent tube, first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the size of the 4th fluorescent tube, model are identical, and are distributed in the surrounding of CCD uniformly and equidistantly.
2. AFB in on-line checkingi corn according to claim 1 1detector, it is characterized in that, CCD is also connected with computer.
3. AFB in on-line checkingi corn according to claim 1 1detector, it is characterized in that, fluorescent tube adopt wavelength be the uviol lamp of 365nm.
CN201520922962.2U 2015-11-19 2015-11-19 Aflatoxin B1's detector in on -line measuring maize Active CN205139016U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520922962.2U CN205139016U (en) 2015-11-19 2015-11-19 Aflatoxin B1's detector in on -line measuring maize

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Application Number Priority Date Filing Date Title
CN201520922962.2U CN205139016U (en) 2015-11-19 2015-11-19 Aflatoxin B1's detector in on -line measuring maize

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CN205139016U true CN205139016U (en) 2016-04-06

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105334199A (en) * 2015-11-19 2016-02-17 无锡百奥森科技有限公司 Detector for on-line detection of aflatoxin B1 in corn and detection method of detector

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105334199A (en) * 2015-11-19 2016-02-17 无锡百奥森科技有限公司 Detector for on-line detection of aflatoxin B1 in corn and detection method of detector

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Address after: Building 214070 West Liyuan Development Zone in Jiangsu province Wuxi City No. 599 Building 1 Room 305

Patentee after: 100 Olson (Jiangsu) Food Safety Technology Co. Ltd.

Address before: Building 214070 West Liyuan Development Zone in Jiangsu province Wuxi City No. 599 Building 1 Room 305

Patentee before: Wuxi Baiaosen Technology Co., Ltd.