CN105334199A - Detector for on-line detection of aflatoxin B1 in corn and detection method of detector - Google Patents
Detector for on-line detection of aflatoxin B1 in corn and detection method of detector Download PDFInfo
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- CN105334199A CN105334199A CN201510797987.9A CN201510797987A CN105334199A CN 105334199 A CN105334199 A CN 105334199A CN 201510797987 A CN201510797987 A CN 201510797987A CN 105334199 A CN105334199 A CN 105334199A
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- corn
- fluorescent tube
- afb
- ccd
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Abstract
The invention discloses a detector for on-line detection of aflatoxin B1 in corn and a detection method of the detector. The detector comprises a CCD, lamp tubes, a conveying belt and an equipment outer casing, wherein the conveying belt penetrates through the equipment outer casing; the equipment outer casing above the conveying belt is a sealing outer casing; the CCD and the lamp tubes are mounted at the top end of the inside of the equipment outer casing; the CCD is located in the center of the top of the equipment outer casing; the lamp tubes are uniformly distributed around the CCD at an equal interval. Known corn samples which are in different concentration and contain aflatoxin B1 are detected by the detector, and the positive correlation relationship between the concentration of aflatoxin B1 and fluorescence values is obtained. Digital image signals of to-be-detected corn are measured, and the concentration of aflatoxin B1 in the current corn is calculated according to the positive correlation relationship between the concentration of aflatoxin B1 and the fluorescence values. Aflatoxin B1 on surfaces of different pieces of corn is directly and continuously detected, the detection cost is reduced greatly, and the detection speed is increased.
Description
Technical field
The present invention relates to microorganism detection field, specifically AFB in a kind of on-line checkingi corn
1detector and detection method.
Background technology
AFB
1be the secondary metabolite that Aspergillus flavus and aspergillus parasiticus bacterium etc. produce at occurring in nature, its toxicity is large, and carcinogenicity is strong, and physicochemical property is relatively stable, is one of most extensive chemical carcinogen found at present.AFB
1shift between biosome by food chain, not only affect production and the processing of various agricultural product, affect output and the benefit of agricultural product, and serious have impact on the healthy of people.
Detect AFB
1method mainly contain thin-layered chromatography, high performance liquid chromatography, euzymelinked immunosorbent assay (ELISA) etc.But thin-layered chromatography complex operation, consuming time, the impact that is subject to each factor in analytic process, the accuracy of method is very poor; High performance liquid chromatography can detect multiple toxin simultaneously, and sensitivity is higher, but requires that sample purity is high, expensive equipment, is difficult to popularize; There is the shortcoming such as false positive, false negative in euzymelinked immunosorbent assay (ELISA).
Utilize AFB
1the characteristic of natural fluoresence can be sent under the irradiation of ultraviolet light, excite the fluorescence of generation to detect to it.It is fast that this detection method has speed, and can the feature of accurate quantitative analysis.Fluorescence detector is used to detect the AFB of ultramicron
1, be widely used for the highly sensitive detection method as aflatoxin.But, adopt Fluorometric assay usually to need and the method coupling such as high performance liquid chromatography, pre-treatment isolation technics.Immune affinity column method, often as the earlier stage processing method of the method, this adds increased testing cost and experimental procedure.
Summary of the invention
The object of the present invention is to provide a kind of reduce testing cost, improve detection speed on-line checkingi corn in AFB
1detector and detection method, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
AFB in a kind of on-line checkingi corn
1detector, comprise CCD, fluorescent tube, transport tape and device housings; Transport tape runs through device housings, and the device housings above transport tape is can; CCD, fluorescent tube are arranged on the top of device housings inside, and CCD is positioned at device housings top center, fluorescent tube comprises the first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the 4th fluorescent tube, first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the size of the 4th fluorescent tube, model are identical, and are distributed in the surrounding of CCD uniformly and equidistantly.
As the further scheme of the present invention: CCD is also connected with computer.
As the further scheme of the present invention: fluorescent tube adopts wavelength to be the uviol lamp of 365nm.
AFB in a kind of on-line checkingi corn
1detection method, comprise the following steps:
1) by known variable concentrations containing AFB
1corn sample, by detector, utilize under different parameters CCD gather AFB
1data image signal, then carry out data analysis, draw AFB
1the positive correlation of concentration and fluorescent value; Different parameters is analog gain respectively: 2 ~ 8; Time shutter: 100 ~ 450; G/G+B:20% ~ 60%;
2) be placed on transport tape by corn to be measured, the data image signal of CCD continuous acquisition corn to be measured also transfers on computer, passes through AFB
1the positive correlation of concentration and fluorescent value calculates the concentration of the aflatoxin B1 of current corn.
Compared with prior art, the invention has the beneficial effects as follows:
AFB in on-line checkingi corn in the present invention
1detector belong to fluorescence detection, it is the AFB of direct-detection sample surfaces
1, and do not need to carry out sample the steps such as pre-treatment, greatly reduce testing cost, improve detection speed, the present invention simultaneously continuously can detect the AFB in different corn
1concentration, and do not affect other samples.
Accompanying drawing explanation
Fig. 1 is AFB in on-line checkingi corn
1the structural representation of detector;
In figure: 1-first fluorescent tube, 2-second fluorescent tube, 3-CCD, 4-the 3rd fluorescent tube, 5-the 4th fluorescent tube, 6-transport tape, 7-device housings.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
Refer to Fig. 1, in the embodiment of the present invention, AFB in a kind of on-line checkingi corn
1detector, comprise CCD3, fluorescent tube, transport tape 6 and device housings 7.Transport tape 6 runs through device housings 7, and the device housings 7 above transport tape 6 is can.CCD3, fluorescent tube are arranged on the top of device housings 7 inside, and CCD3 is positioned at device housings 7 top center, fluorescent tube comprises the first fluorescent tube 1, second fluorescent tube 2, the 3rd fluorescent tube 4, the 4th fluorescent tube 5, first fluorescent tube 1, second fluorescent tube 2, the 3rd fluorescent tube 4, the 4th fluorescent tube 5 size, model are identical, and are distributed in the surrounding of CCD3 uniformly and equidistantly.Fluorescent tube adopts wavelength to be the uviol lamp of 365nm.CCD3 is also connected with computer.
Above-mentioned detector is used for AFB in on-line checkingi corn
1detection method, concrete steps are as described below:
1. transport tape 6 drives corn to be measured average rate from device housings 7 to flow through, and has AFB in corn
1part can send blue-green fluorescent under ultra violet lamp, the power (light intensity) of fluorescence has reacted AFB
1the number of concentration, light intensity and concentration correlation.
2. by the corn sample of variable concentrations, gather relevant data image signal by CCD3, computer reads fluorescent value in analysing digital image signal and and AFB
1concentration sets up linear relationship curve.
3. on high speed high definition CCD3 continuous acquisition transport tape 6 corn data image signal and transfer on computer, pass through AFB
1the positive correlation of concentration and fluorescent value calculates the AFB of current corn
1concentration and compare with pre-set threshold value to identify whether current corn exceeds standard.
4. the concentration data of current corn is communicatively real-time transmitted to host computer, and the information that will exceed standard is transferred to digital switch quantity form the sample processing device that exceeds standard.
CCD in the present invention, (Charge-coupledDevice, charge coupled cell), can be called ccd image sensor, also be image controller.CCD is a kind of semiconductor devices, optical image can be converted into digital signal.The small photoactive substance of the upper implantation of CCD is called pixel (Pixel).The pixel count that one piece of CCD comprises is more, and its screen resolution provided is also higher.The effect of CCD is just as film, but it is that light signal is converted to charge signal.CCD there is the photodiode of many marshallings, can light be responded to, and light signal is transformed into electric signal, convert data image signal to through external sampling amplification and analog to digital conversion circuit.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this instructions is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of instructions is only for clarity sake, those skilled in the art should by instructions integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (4)
1. AFB in an on-line checkingi corn
1detector, it is characterized in that, comprise CCD, fluorescent tube, transport tape and device housings; Transport tape runs through device housings, and the device housings above transport tape is can; CCD, fluorescent tube are arranged on the top of device housings inside, and CCD is positioned at device housings top center, fluorescent tube comprises the first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the 4th fluorescent tube, first fluorescent tube, the second fluorescent tube, the 3rd fluorescent tube, the size of the 4th fluorescent tube, model are identical, and are distributed in the surrounding of CCD uniformly and equidistantly.
2. AFB in on-line checkingi corn according to claim 1
1detector, it is characterized in that, CCD is also connected with computer.
3. AFB in on-line checkingi corn according to claim 1
1detector, it is characterized in that, fluorescent tube adopt wavelength be the uviol lamp of 365nm.
4. one kind as arbitrary in claim 1-3 as described in detector on-line checkingi corn in AFB
1detection method, it is characterized in that, comprise the following steps:
1) by known variable concentrations containing AFB
1corn sample, by detector, utilize under different parameters CCD gather AFB
1data image signal, then carry out data analysis, draw AFB
1the positive correlation of concentration and fluorescent value; Different parameters is analog gain respectively: 2 ~ 8; Time shutter: 100 ~ 450; G/G+B:20% ~ 60%;
2) be placed on transport tape by corn to be measured, the data image signal of CCD continuous acquisition corn to be measured also transfers on computer, passes through AFB
1the positive correlation of concentration and fluorescent value calculates the AFB of current corn
1concentration.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510797987.9A CN105334199A (en) | 2015-11-19 | 2015-11-19 | Detector for on-line detection of aflatoxin B1 in corn and detection method of detector |
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| CN201510797987.9A CN105334199A (en) | 2015-11-19 | 2015-11-19 | Detector for on-line detection of aflatoxin B1 in corn and detection method of detector |
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| CN105334199A true CN105334199A (en) | 2016-02-17 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112362607A (en) * | 2018-11-29 | 2021-02-12 | 平顶山市畜产品质量安全监测中心 | Detection cabinet for aflatoxin in corn feed stored in feedlot |
| CN113418896A (en) * | 2021-05-18 | 2021-09-21 | 中国农业大学 | Method for on-line high-throughput detection of aflatoxin in corn grains |
| CN115769067A (en) * | 2021-06-11 | 2023-03-07 | 东洋坚果食品株式会社 | Pistachio nut sorting device |
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| CN101180537A (en) * | 2005-05-20 | 2008-05-14 | 格林威治大学 | Device for detection and measurement of a target compound such as a food toxin |
| CN101393208A (en) * | 2008-10-07 | 2009-03-25 | 江苏省原子医学研究所 | Light induced chemiluminescent immunoassay kit of aflatoxin B1 and detecting method thereof |
| CN202351177U (en) * | 2011-11-25 | 2012-07-25 | 遵义师范学院 | Aflatoxin detector based on ultraviolet LED light source |
| CN103234945A (en) * | 2013-03-29 | 2013-08-07 | 合肥美亚光电技术股份有限公司 | Aflatoxin detecting and sorting apparatus |
| CN205139016U (en) * | 2015-11-19 | 2016-04-06 | 无锡百奥森科技有限公司 | Aflatoxin B1's detector in on -line measuring maize |
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2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101180537A (en) * | 2005-05-20 | 2008-05-14 | 格林威治大学 | Device for detection and measurement of a target compound such as a food toxin |
| CN101393208A (en) * | 2008-10-07 | 2009-03-25 | 江苏省原子医学研究所 | Light induced chemiluminescent immunoassay kit of aflatoxin B1 and detecting method thereof |
| CN202351177U (en) * | 2011-11-25 | 2012-07-25 | 遵义师范学院 | Aflatoxin detector based on ultraviolet LED light source |
| CN103234945A (en) * | 2013-03-29 | 2013-08-07 | 合肥美亚光电技术股份有限公司 | Aflatoxin detecting and sorting apparatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112362607A (en) * | 2018-11-29 | 2021-02-12 | 平顶山市畜产品质量安全监测中心 | Detection cabinet for aflatoxin in corn feed stored in feedlot |
| CN113418896A (en) * | 2021-05-18 | 2021-09-21 | 中国农业大学 | Method for on-line high-throughput detection of aflatoxin in corn grains |
| CN115769067A (en) * | 2021-06-11 | 2023-03-07 | 东洋坚果食品株式会社 | Pistachio nut sorting device |
| CN115769067B (en) * | 2021-06-11 | 2024-03-12 | 东洋坚果食品株式会社 | Pistachio sorting device |
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Address after: Building 214070 West Liyuan Development Zone in Jiangsu province Wuxi City No. 599 Building 1 Room 305 Applicant after: 100 Olson (Jiangsu) Food Safety Technology Co. Ltd. Address before: Building 214070 West Liyuan Development Zone in Jiangsu province Wuxi City No. 599 Building 1 Room 305 Applicant before: Wuxi Baiaosen Technology Co., Ltd. |
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Application publication date: 20160217 |
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