CN205133609U - Real -time fluorescence quantitative PCR's dual wavelength fluorescence detection device - Google Patents
Real -time fluorescence quantitative PCR's dual wavelength fluorescence detection device Download PDFInfo
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- CN205133609U CN205133609U CN201520814415.2U CN201520814415U CN205133609U CN 205133609 U CN205133609 U CN 205133609U CN 201520814415 U CN201520814415 U CN 201520814415U CN 205133609 U CN205133609 U CN 205133609U
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Abstract
The utility model relates to a real -time fluorescence quantitative PCR's dual wavelength fluorescence detection device, arouse module, glimmering optical receiver module, refraction module including fixing base, light source, it is excited module, glimmering optical receiver module, is realized the fluorescence detection device of dual wavelength when the refraction module is formed by the light source to be equipped with two sets in the fixing base. The light source arouses module, glimmering optical receiver module and refraction module all to become cylindric to insert respectively in the corresponding round hole of fixing base. The utility model discloses a two access structure, the fluorescence signal that can two kinds of wavelength of simultaneous measurement has improved the work efficiency of instrument, has enlarged the range of application of instrument, the binary channels is partd, mutual noninterference, simple structure, handling ease and assembly to the cost is reduced, whole compact structure, it is small, saved the space of instrument, adopt the drum structure for the light path alignment is good, and the testing result degree of accuracy is high.
Description
Technical field
The utility model relates to the detection technique of a kind of biology and medical science, particularly a kind of double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR.
Background technology
Round pcr is the DNA replication dna technology in bio-science application, achieves the significantly amplification of DNA, and achieves the quantitative analysis of DNA profiling based on the real-time fluorescence quantitative PCR instrument of this technology, is all significant to molecular biology and medical research etc.Fluorescence detection device is one of core of real-time fluorescence quantitative PCR instrument.The external detection of the real-time fluorescence of PCR analysis all realizes automatization, and unattended operation eliminates personal errors, improves accuracy, but physical construction and light path complexity, expensive; And domestic product structure is relatively simple, but the property indices such as accuracy, efficiency lags behind international product, and also lacking competitiveness in market, finally makes PCR instrument popularity rate low, greatly have impact on carrying out of this field scientific research and practice.
The real-time PCR of current main flow is the detection of Single wavelength fluorescence, and can only survey a kind of fluorescence of wavelength, its detection efficiency is low, and greatly limit the use range of instrument.And existing fluorescence detection device mainly square structure, the error caused due to mismachining tolerance, assembling error and when using, makes it be difficult to ensure the right alignment of light path cause very large impact to detected result.
Summary of the invention
The utility model for above-mentioned problems of the prior art, propose a kind of fast, accurately, the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR that precision is high.
For solving the problems of the technologies described above, the utility model is achieved through the following technical solutions:
A kind of double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR, comprise permanent seat 1, light source activation module, fluorescence reception module, refractive power module, described permanent seat is realized the fluorescence detection device of dual wavelength while forming by light source activation module, fluorescence reception module, refractive power module built with two covers.
Described light source activation module, fluorescence reception module and refractive power module all become cylindric, and insert respectively in the corresponding circular hole of permanent seat.
Described light source activation module comprises LED light source, collimating lens, exciter filter, dichroscope, light source circular cylindrical shell, described LED light source is placed in light source circular cylindrical shell upper grooves, LED circuit board is equipped with in light source circular cylindrical shell upper end, collimating lens, exciter filter, dichroscope is provided with successively below LED light source, wherein, distance between described collimating lens and exciter filter is takeed on by the hole in light source circular cylindrical shell, back-up ring, holding screw are fixed, and described dichroscope is attached in the groove of light source circular cylindrical shell with 45 °.
Described fluorescence reception module comprises photodiode, receives collimating lens, launches spectral filter, receives circular cylindrical shell, described photodiode is placed in the upper grooves receiving circular cylindrical shell, receive circular cylindrical shell upper end and circuit card is housed, be provided with successively below photodiode and receive collimating lens, launch spectral filter
Described photodiode and receive between collimating lens, the distance received between collimating lens and transmitting spectral filter is takeed on by hole respectively, back-up ring, holding screw are fixed.
Described refractive power module comprises the circular cylindrical shell being provided with light hole, and described circular cylindrical shell internal fixtion has condenser lens and prism.
Feedback diode is equipped with in LED light source side in described light source activation module.
Compared to prior art, the utility model has following beneficial effect:
1. channel structure, can measure the fluorescent signal of two kinds of wavelength simultaneously, improve the working efficiency of instrument, expand the range of application of instrument;
2. two channels separately, does not interfere with each other;
3. structure is simple, is convenient to processing and assembling, and reduces cost;
4. total is compact, and volume is little, saves the space of instrument;
5. cylindrical structure, make light path coaxial good, detected result accuracy is high;
Certainly, implement arbitrary product of the present utility model might not need to reach above-described all advantages simultaneously.
Accompanying drawing explanation
Fig. 1 is construction profile schematic diagram of the present utility model;
Fig. 2 is main sectional view of the present utility model;
Fig. 3 is refractive power module sectional view of the present utility model.
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present utility model is described further:
As shown in Figure 1 to Figure 3, a kind of double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR mainly comprises permanent seat 1, first and second light source activation module 2,5, first and second fluorescence reception module 3,4 and first and second refractive power module 6,7.
First and second light source activation module 2,5 is equal modules, and first and second fluorescence reception module 3,4 is equal modules, and first and second refractive power module 6,7 is equal modules.
Permanent seat 1 is realized the fluorescence detection device of dual wavelength while forming by light source activation module, fluorescence reception module, refractive power module built with two covers.
Two light source activation modules, two fluorescence reception modules and two refractive power modules all become cylindric, and insert respectively in the corresponding circular hole of permanent seat 1, and make light path coaxial good, detected result accuracy is high.
Specifically, first light source activation module 2 comprises the first LED light source 10, first collimating lens 12, first exciter filter 13, first dichroscope 14, first light source circular cylindrical shell 8, first LED light source 10 is placed in the first light source circular cylindrical shell 8 upper grooves, first light source circular cylindrical shell 8 upper end is equipped with below the first LED circuit board 9, first LED light source 10 and is provided with the first collimating lens 12, first exciter filter 13, first dichroscope 14 successively.
Equally, secondary light source excitation module 5 comprises the second LED light source 27, second collimating lens 29, second exciter filter 30, second dichroscope 31, secondary light source circular cylindrical shell 25, second LED light source 27 is placed in secondary light source circular cylindrical shell 25 upper grooves, secondary light source circular cylindrical shell 25 upper end is equipped with below the second LED circuit board 26, second LED light source 27 and is provided with the second collimating lens 29, second exciter filter 30, second dichroscope 31 successively.
First fluorescence reception module 3 comprises the first photodiode 17, first and receives collimating lens 18, first transmitting spectral filter 19, first reception circular cylindrical shell 15, first photodiode 17 is placed in the upper grooves of the first reception circular cylindrical shell 15, first receives circular cylindrical shell 15 upper end is equipped with first circuit board 16, is provided with the first reception collimating lens 18, first successively and launches spectral filter 19 below the first photodiode 17.
Equally, second fluorescence reception module 4 comprises the second photodiode 22, second and receives collimating lens 23, second transmitting spectral filter 24, second reception circular cylindrical shell 20, second photodiode 22 is placed in the upper grooves of the second reception circular cylindrical shell 20, second receives circular cylindrical shell 20 upper end is equipped with second circuit board 21, is provided with the second reception collimating lens 23, second successively and launches spectral filter 24 below the second photodiode 22.
First refractive power module 6 comprises the first circular cylindrical shell 32, first circular cylindrical shell 32 internal fixtion being provided with light hole the first condenser lens 33 and the first prism 34.
Equally, the second refractive power module 7 comprises the second circular cylindrical shell 35, second circular cylindrical shell 35 internal fixtion being provided with light hole the second condenser lens 36 and the second prism 37.
In light source activation module, the light that LED light source is launched, incide on dichroscope after collimating lens collimation, light incides on the prism in refractive power module after dichroic mirror reflects, light is directive condenser lens after prism total reflection, incident light edge through focusing on is injected in test sample through described light hole in direction straight up, fluorophor in the test sample fluorescence produced that is excited is transmitted on prism via described light hole directive condenser lens, fluorescence is injected into dichroscope after prism total reflection, transmission fluorescence directive through dichroscope launches spectral filter, through launching the fluorescence of filter sheet, received by photodiode after collimating lens.The fluorescent signal received, through microprocessor processes, according to the threshold value automatic decision yin and yang attribute result arranged.
The light that light source described in the utility model is launched is injected on dichroscope with 45 °, and light impinges perpendicularly on prism after dichroic mirror reflects.
Further, first and second light source activation module 2, in 5, the side of first and second LED light source 10,27 is corresponding is equipped with first and second feedback diode 11,28, and feedback diode is used for detecting the power of light, thus the light intensity controlling light source keeps constant.
In light source activation module of the present utility model, the top of light source circular cylindrical shell mills out groove, ensures the installation of LED and feedback diode.LED light source, distance between collimating lens and exciter filter are takeed on by hole, back-up ring adds holding screw and ensures.Dichroscope 45 ° is attached in light source circular cylindrical shell groove.
In fluorescence reception module of the present utility model, the top receiving circular cylindrical shell mills out groove, ensures the installation of photodiode.Photodiode, distance between collimating lens and transmitting spectral filter are takeed on by hole, back-up ring adds holding screw and ensures.
Claims (6)
1. the double wave length fluorescent proofing unit of a real-time fluorescence quantitative PCR, comprise permanent seat (1), light source activation module, fluorescence reception module, refractive power module, it is characterized in that: described permanent seat (1) is realized the fluorescence detection device of dual wavelength while forming by light source activation module, fluorescence reception module, refractive power module built with two covers.
2. the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR according to claim 1, is characterized in that: described light source activation module, fluorescence reception module and refractive power module all become cylindric, and insert respectively in the corresponding circular hole of permanent seat (1).
3. the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR according to claim 1, it is characterized in that: described light source activation module comprises LED light source, collimating lens, exciter filter, dichroscope, light source circular cylindrical shell, described LED light source is placed in light source circular cylindrical shell upper grooves, LED circuit board is equipped with in light source circular cylindrical shell upper end, collimating lens is provided with successively below LED light source, exciter filter, dichroscope, wherein, distance between described collimating lens and exciter filter is takeed on by the hole in light source circular cylindrical shell, back-up ring, holding screw is fixed, described dichroscope is attached in the groove of light source circular cylindrical shell with 45 °.
4. the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR according to claim 1, it is characterized in that: described fluorescence reception module comprises photodiode, receive collimating lens, launch spectral filter, receive circular cylindrical shell, described photodiode is placed in the upper grooves receiving circular cylindrical shell, receive circular cylindrical shell upper end and circuit card is housed, reception collimating lens is provided with successively below photodiode, launch spectral filter, between described photodiode and reception collimating lens, the distance received between collimating lens and transmitting spectral filter is takeed on by hole respectively, back-up ring, holding screw is fixed.
5. the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR according to claim 1, is characterized in that: described refractive power module comprises the circular cylindrical shell being provided with light hole, and described circular cylindrical shell internal fixtion has condenser lens and prism.
6. the double wave length fluorescent proofing unit of real-time fluorescence quantitative PCR according to claim 3, is characterized in that: feedback diode is equipped with in the LED light source side in described light source activation module.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520814415.2U CN205133609U (en) | 2015-10-21 | 2015-10-21 | Real -time fluorescence quantitative PCR's dual wavelength fluorescence detection device |
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| CN201520814415.2U CN205133609U (en) | 2015-10-21 | 2015-10-21 | Real -time fluorescence quantitative PCR's dual wavelength fluorescence detection device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389644A (en) * | 2017-08-11 | 2017-11-24 | 长春理工大学 | A kind of rapid fluorescence proportioning device |
| CN107421935A (en) * | 2017-08-11 | 2017-12-01 | 长春理工大学 | A kind of fluorescence micro RNA detection means and detection method |
| CN112649411A (en) * | 2020-12-25 | 2021-04-13 | 中国科学院苏州生物医学工程技术研究所 | Multi-wave long-time resolution fluorescence measuring device |
-
2015
- 2015-10-21 CN CN201520814415.2U patent/CN205133609U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389644A (en) * | 2017-08-11 | 2017-11-24 | 长春理工大学 | A kind of rapid fluorescence proportioning device |
| CN107421935A (en) * | 2017-08-11 | 2017-12-01 | 长春理工大学 | A kind of fluorescence micro RNA detection means and detection method |
| CN112649411A (en) * | 2020-12-25 | 2021-04-13 | 中国科学院苏州生物医学工程技术研究所 | Multi-wave long-time resolution fluorescence measuring device |
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