CN205133576U - Mobile chamber for biological fluid experiments - Google Patents

Mobile chamber for biological fluid experiments Download PDF

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Publication number
CN205133576U
CN205133576U CN201520769563.7U CN201520769563U CN205133576U CN 205133576 U CN205133576 U CN 205133576U CN 201520769563 U CN201520769563 U CN 201520769563U CN 205133576 U CN205133576 U CN 205133576U
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CN
China
Prior art keywords
bottom sheet
fluid
turnover
tubule
flow cavity
Prior art date
Application number
CN201520769563.7U
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Chinese (zh)
Inventor
徐紫宸
王贵学
徐文峰
廖晓玲
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重庆大学
重庆科技学院
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Application filed by 重庆大学, 重庆科技学院 filed Critical 重庆大学
Priority to CN201520769563.7U priority Critical patent/CN205133576U/en
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Publication of CN205133576U publication Critical patent/CN205133576U/en

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Abstract

The utility model provides a mobile chamber for biological fluid experiments, last piece that the chamber includes that the level personally submits the rectangle and piece down flow to and set gradually the horizontal plane by last piece to piece down and be rectangle and long wide measure -alike last film trap seal ring, cell slide glass, flow chamber seal ring and glass mounting, on its slice and lower piece pass through bolted connection, the beneficial effects of the utility model are that: the utility model discloses a mobile chamber for biological fluid experiments simple structure, convenient to use, and simultaneously, the utility model discloses the practicality is stronger, can realize the change of mobile cavity size through the mobile chamber seal ring's of simple adjustment thickness to reach nimble changeable effect, adapt to various different kinds of experiment requirements, especially, in the import and the export of the mobile cavity of business turn over, the utility model discloses special design what buffering uniform flow structure, the fluid business turn over is all through buffering uniform flows at different levels in the experiment for the experimental data is more accurate, and the experiment effect is better.

Description

A kind of biofluid experiment flow cavity

Technical field

The utility model relates to a kind of biomedicine experiment device, particularly a kind of biofluid experiment flow cavity.

Background technology

Biofluid experiment flow cavity is exactly can by the experimental part of the flow chamber of microscopic examination.In biomedical engineering experimental study, for the various kinds of cell such as endotheliocyte, blood cell of blood vessel, under hydrokinetic effect, the experimental study of its cell proliferation, adhesion etc. has been carried out in a deep going way; But due to the singularity of angioarchitecture, add that the hydromeehanics of research is different with the special emphasis of blood vessel, above-mentioned experiment all needs design personalized laboratory apparatus; And in the market because the personalization studied is strong for this reason, almost there is no general blood vessel bionic cell flow laboratory apparatus; Most of research institution is all according to respective experiment needs, the laboratory apparatus of designed, designed; Biofluid experiment flow cavity is the core part of experiment porch in fluid experiment instrument; It is to Success in Experiment whether judgement, experimental design, the direct judgement of Rational Parameters, and the direct acquisition of experimental cell image and experimental data all serves vital effect; And, varying in size for experiment porch, the liquid circulation amount needed for Fluid Mechanics experiment and the requirement of flow velocity, the flow chamber of the flow cavity of needs varies in size, therefore needs a kind of biofluid experiment of specialized designs flow cavity, meets requirement of experiment; And for custom-designed a kind of cell fluid mechanics, to test special cell flow laboratory apparatus supporting, and make each experimentation cost higher, custom-designed flow cavity and necessary instrument do not have versatility, and service efficiency is low; In a word, biofluid experiment flow cavity of the prior art also exists poor universality, the low inferior problem of service efficiency.

Utility model content

In view of this, the purpose of this utility model is to provide a kind of biofluid experiment flow cavity.

Realize above-mentioned purpose, the technical solution adopted in the utility model is: a kind of biofluid experiment flow cavity, described flow cavity comprise horizontal plane rectangular upper slice and bottom sheet, and set gradually horizontal plane by upper slice to bottom sheet and be rectangle and measure-alike upper film trap seal washer, cell slide glass, flow cavity seal washer and the glass mounting of length and width, on it slice and bottom sheet be bolted, described upper slice be provided with an opening, upper film trap and upper slice bolt hole, on it, an opening is through is arranged at a medium position, upper film trap was along upper slice edge of opening arranged outside one week, notch is downward, its edge length and width size and upper film trap seal washer size suitable, upper slice bolt hole is arranged at outside film trap, upper film trap seal washer arranges one week along upper slice groove edge, described cell slide glass is loaded with cell side and arranges downwards, described flow cavity seal washer arranges one week along cell slide glass edge, and its central space is flow chamber, described glass mounting is fixedly installed in bottom sheet, and glass mounting minor face two ends are symmetrically arranged with up/down perforation and the mounting fluid be communicated with flowing cavity passes in and out groove, and this mounting fluid turnover groove and glass mounting minor face be arranged in parallel, described bottom sheet is provided with top boss, bottom sheet opening, two bottom sheet fluid turnover grooves, two advection passages, two turnover even flowing chute, four fluid tubules and bottom sheet bolt hole, top boss horizontal plane is rectangular is arranged at bottom sheet end face medium position, the length and width size of top boss is identical with glass mounting size, bottom sheet opening is arranged at top boss medium position, the whole bottom sheet of its up/down perforation is also corresponding with upper slice aperture position, two bottom sheet fluid turnover grooves are symmetricly set in boss minor face two ends, top respectively, itself and mounting fluid pass in and out the wide consistent size of flute length and correspondingly to arrange, two turnover even flowing chute to be arranged at respectively outside two bottom sheet fluids turnover grooves and all to pass in and out groove with two bottom sheet fluids and be arranged in parallel, two turnover even flowing chute are all communicated with two bottom sheet fluid turnover grooves respectively by two advection passages, bottom two turnover even flowing chute, two ends are respectively provided with one group of tubule hole, described four fluid tubules are arranged in bottom sheet, each fluid tubule one end connects one group of tubule hole connection turnover even flowing chute by corresponding, the other end is communicated to outside bottom sheet respectively, and the fluid tubule the other end two being communicated with different turnover even flowing chute passes through upwards to arch upward in the soft pipe connection of rainbow shape, two other fluid tubule the other end is fluid inlet tube mouth, and one is fluid outlet spout.

Further, the fluid tubule that every two one end of described four fluid tubules are communicated with different turnover even flowing chute is one group, and two groups of fluid tubule the other ends are communicated to the symmetrical both sides of bottom sheet respectively;

Further, described tubule hole of often organizing comprises a tubule macropore and a tubule aperture, and tubule macropore is arranged near turnover even flowing chute medium position, and tubule aperture is arranged near turnover even flowing chute end position;

Further, described bottom sheet fluid turnover groove and advection passage length consistent size;

Further, described turnover uniform flow groove depth 3mm ~ 5mm, wide 2mm ~ 3mm, fluid turnover groove depth 3mm ~ 5mm, wide 2mm ~ 3mm, the dark 1mm of advection passage;

Further, the thickness of described flow cavity seal washer is 0.01mm ~ 2mm;

Further, four angles of described upper film trap are provided with bight and reserve groove;

Further, described upper slice and the length and width consistent size of bottom sheet, length and width ratio is between 1.4:1 ~ 1.6:1; Upper slice thickness is 6mm ~ 10mm, and bottom sheet thickness is 5mm ~ 6mm;

Further, the symmetric line of described top boss overlaps with bottom sheet symmetric line, and length and width direction is consistent, and its thickness is 1mm ~ 5mm, with each limit of bottom sheet at a distance of 5mm ~ 10mm;

Further, the symmetric line of described upper slice opening overlaps with top boss symmetric line, and length and width direction is consistent, upper slice long limit of opening and the long back gauge 5mm ~ 8mm with side roof part boss, upper slice minor face of opening and the minor face distance 10mm ~ 25mm with side roof part boss.

The beneficial effects of the utility model are: biofluid experiment flow cavity structure of the present utility model is simple, easy to use, it is by upper and lower, and a series of simple part combination arranged between upper and lower, defines biofluid experiment flow cavity structure quickly and efficiently, meanwhile, the utility model practicality is comparatively strong, by the thickness of simple adjustment flow cavity seal washer, can realize the change of flow chamber size, thus reach flexible and changeable effect, adapt to various dissimilar requirement of experiment, especially, in import and the outlet of turnover flow chamber, what buffering flow configuration of the utility model specialized designs, first, Experimental Flowing Object of the present utility model non-immediate directly imports and exports flow cavity by fluid tubule, the fluid tubule being responsible for importing and exporting Experimental Flowing Object is all communicated with turnover even flowing chute by tubule hole group, turnover even flowing chute is again by advection channel connection bottom sheet fluid turnover groove, finally by passing in and out with mounting fluid, groove is corresponding to be arranged thus communication of fluid chamber bottom sheet fluid turnover groove, whole Experimental Flowing Object turnover is all by aforementioned buffering uniform flow at different levels, make experimental data more accurate, experiment effect is better, secondly, the utility model is also by the design of following details, further increase the effect of buffering uniform flow, namely mounting fluid turnover groove and glass mounting minor face be arranged in parallel, two bottom sheet fluid turnover grooves are symmetricly set in boss minor face two ends, top respectively, itself and mounting fluid pass in and out the wide consistent size of flute length and correspondingly to arrange, and two turnover even flowing chute are arranged at two bottom sheet fluids respectively and pass in and out and also all pass in and out groove with two bottom sheet fluids outside grooves and be arranged in parallel, in a word, the utility model is simple relative to the flow cavity structure of prior art, and highly versatile, can adapt to the needs of various different experiments type, and experimental precision and effect better.

Accompanying drawing explanation

Below in conjunction with the drawings and specific embodiments, the utility model is further described:

Fig. 1 is the utility model flow cavity cross-sectional view;

Fig. 2 is the utility model upper slice upward view;

Fig. 3 is A-A cross-sectional view in Fig. 2;

Fig. 4 is the utility model bottom sheet upward view;

Fig. 5 is the utility model bottom sheet vertical view;

Fig. 6 is B-B cross-sectional view in Fig. 5;

In figure: 1. bottom sheet; 2. bottom sheet opening; 3. bottom sheet bolt hole; 4. fluid tubule; 5. top boss; 6. glass mounting; 7. go up a bolt hole; 8. fluid turnover groove; 9. pass in and out even flowing chute; 10. tubule aperture; 11. tubule macropores; 12. advection passages; 13. upper slice; Film trap on 14.; 15. upper slice openings; Groove is reserved in 16. bights; 17. flow cavity seal washers; Film trap seal washer on 18.; 19. microscope camera lenses; 20. cell slide glasss; 21. flow chamber.

Embodiment

As shown in Figures 1 to 6, a kind of biofluid experiment flow cavity, described flow cavity comprise horizontal plane rectangular upper slice 13 and bottom sheet 1, and 13 set gradually horizontal plane to bottom sheet 1 by upper slice and be rectangle and measure-alike upper film trap 14 seal washer 18, cell slide glass 20, flow cavity seal washer 17 and the glass mounting 6 of length and width, on it slices 13 and bottom sheet 1 be bolted, described upper slice 13 be provided with an opening 15, upper film trap 14 and upper slice bolt hole 7, on it, an opening 15 is through is arranged at 13 medium position, upper film trap 14 was along upper slice opening 15 edge arranged outside one week, notch is downward, its edge length and width size and upper film trap 14 seal washer 18 size suitable, upper slice bolt hole 7 is arranged at outside film trap 14, upper film trap 14 seal washer 18 arranges one week along upper film trap 14 edge, described cell slide glass 20 is loaded with cell side and arranges downwards, described flow cavity seal washer 17 arranges one week along cell slide glass 20 edge, and its central space is flow chamber 21, described glass mounting 6 is fixedly installed in bottom sheet 1, and glass mounting 6 minor face two ends are symmetrically arranged with up/down perforation and the mounting fluid be communicated with flowing cavity passes in and out groove 8, and this mounting fluid turnover groove 8 be arranged in parallel with glass mounting 6 minor face, described bottom sheet 1 is provided with top boss 5, bottom sheet opening 2, two bottom sheet fluid turnover grooves 8, two advection passages 12, two turnover even flowing chute 9, four fluid tubules 4 and bottom sheet bolt hole 3, top boss 5 horizontal plane is rectangular is arranged at bottom sheet 1 end face medium position, the length and width size of top boss 5 is identical with glass mounting 6 size, bottom sheet opening 2 is arranged at top boss 5 medium position, the whole bottom sheet of its up/down perforation 1 is also corresponding with upper slice opening 15 position, two bottom sheet fluid turnover grooves 8 are symmetricly set in top boss 5 minor face two ends respectively, itself and mounting fluid pass in and out groove 8 length and width consistent size and correspondingly to arrange, two turnover even flowing chute 9 to be arranged at respectively outside two bottom sheet fluids turnover grooves 8 and all to pass in and out groove 8 with two bottom sheet fluids and be arranged in parallel, two turnover even flowing chute 9 are all communicated with two bottom sheet fluid turnover grooves 8 respectively by two advection passages 12, bottom two turnover even flowing chute 9, two ends are respectively provided with one group of tubule hole, described four fluid tubules 4 are arranged in bottom sheet 1, each fluid tubule 4 one end connects one group of tubule hole connection turnover even flowing chute 9 by corresponding, the other end is communicated to outside bottom sheet 1 respectively, and fluid tubule 4 the other end two being communicated with different turnover even flowing chute 9 passes through upwards to arch upward in the soft pipe connection of rainbow shape, two other fluid tubule 4 the other end is fluid inlet tube mouth, and one is fluid outlet spout.Biofluid experiment flow cavity structure of the present utility model is simple, easy to use, and it is by upper and lower, and a series of simple part combination arranged between upper and lower, defines biofluid experiment flow cavity structure quickly and efficiently, meanwhile, the utility model practicality is comparatively strong, by the thickness of simple adjustment flow cavity seal washer 17, can realize the change of flow chamber 21 size, thus reach flexible and changeable effect, adapt to various dissimilar requirement of experiment, especially, in import and the outlet of turnover flow chamber 21, what buffering flow configuration of the utility model specialized designs, first, Experimental Flowing Object of the present utility model non-immediate directly imports and exports flow cavity by fluid tubule 4, the fluid tubule 4 being responsible for importing and exporting Experimental Flowing Object is all communicated with turnover even flowing chute 9 by tubule hole group, turnover even flowing chute 9 is communicated with bottom sheet fluid turnover groove 8 by advection passage 12 again, finally by passing in and out with mounting fluid, groove 8 is corresponding to be arranged thus communication of fluid chamber bottom sheet fluid turnover groove 8, whole Experimental Flowing Object turnover is all by aforementioned buffering uniform flow at different levels, make experimental data more accurate, experiment effect is better, secondly, the utility model is also by the design of following details, further increase the effect of buffering uniform flow, namely mounting fluid turnover groove 8 be arranged in parallel with glass mounting 6 minor face, two bottom sheet fluid turnover grooves 8 are symmetricly set in top boss 5 minor face two ends respectively, itself and mounting fluid pass in and out groove 8 length and width consistent size and correspondingly to arrange, and two turnover even flowing chute 9 are arranged at two bottom sheet fluids respectively and pass in and out and also all pass in and out groove 8 with two bottom sheet fluids outside grooves 8 and be arranged in parallel, in a word, the utility model is simple relative to the flow cavity structure of prior art, and highly versatile, can adapt to the needs of various different experiments type, and experimental precision and effect better.

In order to optimize biofluid experiment flow cavity further, the fluid tubule 4 that every two one end of described four fluid tubules 4 are communicated with different turnover even flowing chute 9 is one group, and two groups of fluid tubule 4 the other ends are communicated to the symmetrical both sides of bottom sheet 1 respectively; Fluid tubule 4 is set like this, biofluid is tested easier for installation; Often organize tubule hole and comprise a tubule macropore 11 and a tubule aperture 10, tubule macropore 11 is arranged near turnover even flowing chute 9 medium position, and tubule aperture 10 is arranged near turnover even flowing chute 9 end position; Often organize tubule hole divide sized by arrange, and be placed in rational position, more effectively realize fluid uniform flow effect; Bottom sheet fluid turnover groove 8 is consistent with advection passage 12 length dimension, be provided with the fluid be beneficial in turnover even flowing chute 9 like this when being left to bottom sheet fluid turnover groove 8 by advection passage 12, fluid velocity localized variation can not affected because bottom sheet fluid turnover groove 8 change with advection passage 12 length dimension again; Turnover even flowing chute 9 dark 3mm ~ 5mm, wide 2mm ~ 3mm, fluid turnover groove 8 dark 3mm ~ 5mm, wide 2mm ~ 3mm, the dark 1mm of advection passage 12, arranges uniform flow effect better like this; The thickness of flow cavity seal washer 17 is 0.01mm ~ 2mm, and this thickness can according to the flow cavity seal washer 17 needing selection different of fluid experiment, and the thickness of flow cavity seal washer 17 is thicker, and flow chamber 21 is larger, otherwise less; Four angles of upper film trap 14 are provided with bight and reserve groove 16, arrange more favourable laboratory flow chamber like this and install; Upper slice 13 and the length and width consistent size of bottom sheet 1, length and width ratio is between 1.4:1 ~ 1.6:1; Upper slice 13 thickness are 6mm ~ 10mm, and bottom sheet 1 thickness is 5mm ~ 6mm, upper slice 13 and bottom sheet 1 select rational size and ratio, make flow cavity entirety form brief and practical more; The symmetric line of top boss 5 overlaps with bottom sheet 1 symmetric line, and length and width direction is consistent, and its thickness is 1mm ~ 5mm, with each limit of bottom sheet 1 at a distance of 5mm ~ 10mm; The symmetric line of upper slice opening 15 overlaps with top boss 5 symmetric line, and length and width direction is consistent, long limit and the long back gauge 5mm ~ 8mm with side roof part boss 5 of upper slice opening 15, minor face and the minor face distance 10mm ~ 25mm with side roof part boss 5 of upper slice opening 15, because the position of top boss 5 and upper slice opening 15 is selected and size Selection, whole flow cavity is designed more reasonable.

Slide glass in biofluid experiment flow cavity loads the cell that will observe, fluid tubule 4 fluid inlet tube mouth introduces fluid, is full of whole flow cavity, draws from fluid outlet spout, after form persistent loop in flow cavity, microscope camera lens 19 just can be used from flow cavity top view.

What finally illustrate is, above embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify to the technical solution of the utility model or equivalent replacement, and not departing from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.

Claims (10)

1. a biofluid experiment flow cavity, it is characterized in that: described flow cavity comprise horizontal plane rectangular upper slice and bottom sheet, and set gradually horizontal plane by upper slice to bottom sheet and be rectangle and measure-alike upper film trap seal washer, cell slide glass, flow cavity seal washer and the glass mounting of length and width, on it slice and bottom sheet be bolted, described upper slice be provided with an opening, upper film trap and upper slice bolt hole, on it, an opening is through is arranged at a medium position, upper film trap was along upper slice edge of opening arranged outside one week, notch is downward, its edge length and width size and upper film trap seal washer size suitable, upper slice bolt hole is arranged at outside film trap, upper film trap seal washer arranges one week along upper slice groove edge, described cell slide glass is loaded with cell side and arranges downwards, described flow cavity seal washer arranges one week along cell slide glass edge, and its central space is flow chamber, described glass mounting is fixedly installed in bottom sheet, and glass mounting minor face two ends are symmetrically arranged with up/down perforation and the mounting fluid be communicated with flowing cavity passes in and out groove, and this mounting fluid turnover groove and glass mounting minor face be arranged in parallel, described bottom sheet is provided with top boss, bottom sheet opening, two bottom sheet fluid turnover grooves, two advection passages, two turnover even flowing chute, four fluid tubules and bottom sheet bolt hole, top boss horizontal plane is rectangular is arranged at bottom sheet end face medium position, the length and width size of top boss is identical with glass mounting size, bottom sheet opening is arranged at top boss medium position, the whole bottom sheet of its up/down perforation is also corresponding with upper slice aperture position, two bottom sheet fluid turnover grooves are symmetricly set in boss minor face two ends, top respectively, itself and mounting fluid pass in and out the wide consistent size of flute length and correspondingly to arrange, two turnover even flowing chute to be arranged at respectively outside two bottom sheet fluids turnover grooves and all to pass in and out groove with two bottom sheet fluids and be arranged in parallel, two turnover even flowing chute are all communicated with two bottom sheet fluid turnover grooves respectively by two advection passages, bottom two turnover even flowing chute, two ends are respectively provided with one group of tubule hole, described four fluid tubules are arranged in bottom sheet, each fluid tubule one end connects one group of tubule hole connection turnover even flowing chute by corresponding, the other end is communicated to outside bottom sheet respectively, and the fluid tubule the other end two being communicated with different turnover even flowing chute passes through upwards to arch upward in the soft pipe connection of rainbow shape, two other fluid tubule the other end is fluid inlet tube mouth, and one is fluid outlet spout.
2. biofluid experiment flow cavity according to claim 1, is characterized in that: the fluid tubule that every two one end of described four fluid tubules are communicated with different turnover even flowing chute is one group, and two groups of fluid tubule the other ends are communicated to the symmetrical both sides of bottom sheet respectively.
3. the biofluid experiment flow cavity according to any one of claim 1 or 2, it is characterized in that: described tubule hole of often organizing comprises a tubule macropore and a tubule aperture, tubule macropore is arranged near turnover even flowing chute medium position, and tubule aperture is arranged near turnover even flowing chute end position.
4. biofluid experiment flow cavity according to claim 1, is characterized in that: described bottom sheet fluid turnover groove and advection passage length consistent size.
5. the biofluid experiment flow cavity according to any one of claim 1 or 4, is characterized in that: described turnover uniform flow groove depth 3mm ~ 5mm, wide 2mm ~ 3mm, fluid turnover groove depth 3mm ~ 5mm, wide 2mm ~ 3mm, the dark 1mm of advection passage.
6. biofluid experiment flow cavity according to claim 1, is characterized in that: the thickness of described flow cavity seal washer is 0.01mm ~ 2mm.
7. biofluid experiment flow cavity according to claim 1, is characterized in that: four angles of described upper film trap are provided with bight and reserve groove.
8. biofluid experiment flow cavity according to claim 1, is characterized in that: described upper slice and the length and width consistent size of bottom sheet, length and width ratio is between 1.4:1 ~ 1.6:1; Upper slice thickness is 6mm ~ 10mm, and bottom sheet thickness is 5mm ~ 6mm.
9. biofluid experiment flow cavity according to claim 1, is characterized in that: the symmetric line of described top boss overlaps with bottom sheet symmetric line, and length and width direction is consistent, and its thickness is 1mm ~ 5mm, with each limit of bottom sheet at a distance of 5mm ~ 10mm.
10. biofluid experiment flow cavity according to claim 1, it is characterized in that: the symmetric line of described upper slice opening overlaps with top boss symmetric line, and length and width direction is consistent, long limit and the long back gauge 5mm ~ 8mm with side roof part boss of upper slice opening, upper slice minor face of opening and the minor face distance 10mm ~ 25mm with side roof part boss.
CN201520769563.7U 2015-09-30 2015-09-30 Mobile chamber for biological fluid experiments CN205133576U (en)

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CN201520769563.7U CN205133576U (en) 2015-09-30 2015-09-30 Mobile chamber for biological fluid experiments

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Application Number Priority Date Filing Date Title
CN201520769563.7U CN205133576U (en) 2015-09-30 2015-09-30 Mobile chamber for biological fluid experiments

Publications (1)

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CN205133576U true CN205133576U (en) 2016-04-06

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Country Status (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154315A (en) * 2015-09-30 2015-12-16 重庆大学 Flowing chamber for biofluid experiment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154315A (en) * 2015-09-30 2015-12-16 重庆大学 Flowing chamber for biofluid experiment
CN105154315B (en) * 2015-09-30 2017-12-01 重庆大学 A kind of biofluid experiment flow cavity

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AV01 Patent right actively abandoned
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Granted publication date: 20160406

Effective date of abandoning: 20171201