CN205105573U - Device is sprouted to aseptic stock form method - Google Patents

Device is sprouted to aseptic stock form method Download PDF

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Publication number
CN205105573U
CN205105573U CN201520861990.8U CN201520861990U CN205105573U CN 205105573 U CN205105573 U CN 205105573U CN 201520861990 U CN201520861990 U CN 201520861990U CN 205105573 U CN205105573 U CN 205105573U
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CN
China
Prior art keywords
aseptic
coil paper
sprouting
clamping plate
seed
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Expired - Fee Related
Application number
CN201520861990.8U
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Chinese (zh)
Inventor
沈亚欧
杨云
龙芸
张严玲
张晓玲
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Sichuan Agricultural University
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Sichuan Agricultural University
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Priority to CN201520861990.8U priority Critical patent/CN205105573U/en
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Publication of CN205105573U publication Critical patent/CN205105573U/en
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  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The utility model discloses a device is sprouted to aseptic stock form method, an aseptic stock form for bearing the weight of the seed is equipped with, the grounding case comprises a case body, case lid and at least one splint, the box is non -light tight hollow structure, hollow structure forms and sprouts the chamber, the case lid is that detachable connects on the opening of box and seals sprouting the chamber, at least one bleeder vent has been seted up on the case lid, be provided with aseptic ventilated membrane in the bleeder vent, splint set up in sprouting the intracavity, the centre gripping seam that splint were seted up the bottom entry that supplies aseptic stock form and were fixed, when sprouting the seed, the aseptic stock form that is equipped with the seed is sealed in sprouting the chamber under the effect of case lid, makes its and external fungus class completely cut off, has improved the sprouting rate, simultaneously aseptic ventilated membrane makes it can carry out normal respiration, and translucent construction's box makes it can receive even illumination, consequently uses the utility model discloses when sprouting the seed, can completely cut off external fungus class, sprouting rate height, sprouting time in unison, and easy operation.

Description

Aseptic coil paper method sprouts device
Technical field
The utility model relates to a kind of tissue cultures seedling device, particularly relates to a kind of device of aseptic coil paper method germination seed.
Background technology
At present, regarding assay room seed germination technology mainly contains following three kinds: common coil paper method, sprouting box sprout method and vermiculite sprouting method.
Common coil paper method: one one, seed after sterilization is involved in middle and upper part in filter paper, after wrapping, the bottom of filter paper is put into the Plastic Drum that water is housed, lean against barrel edge, then directly places under illumination and cultivates, with these four days afterwards visible filter paper interbed extract greenery out; The shortcoming of this kind of method is: be directly exposed to external environment easy bacteria-developing, and illumination is bad, but inner bucket water easily evaporates under high light, and the seed between paper easily drops.
Sprout box sprouting to send out: in stainless sprouting box, spread multi-layer filter paper in advance, high-temperature sterilization, in aseptic operating platform, the seed that sterilizing is good is put into after oven dry and sprout box, pour suitably many sterile waters into, cover lid is sprouted visible young shoot two days later and is sprouted, again by sprout after seed put into prepare in advance sprouting MS medium, illumination grow; The shortcoming of this kind of method is: complicated operation, and in sprouting box, the water yield is not easily held, and will affect the priority of seed germination time, makes seed germination time disunity, repeatedly be proceeded in germination medium by the seed moving sprouting.
Vermiculite sprouts method: enter between vermiculite by the seed disinfected, watering every day to put has sprouting in three days under light illumination afterwards as seen; The shortcoming of this kind of method is: be directly exposed to external environment easy bacteria-developing, and evaporation needs water every day too soon, and humidity is unstable and wayward, makes seed germination rate lower and sprout time also disunity.
Separately, seed is in germination process, if be directly exposed in external environment, grow various bacterium (being mainly fungus and bacterium) by the seed making to sprout, seed germination rate can be caused to decline, the seedling simultaneously just sprouted is because long bacterium can make its oligotrophy, and the various harms such as sick generation murder by poisoning, further, the seedling after long bacterium can not use as tissue cultures, wastes human and material resources greatly.
Therefore, need badly a kind ofly can completely cut off extraneous mushroom, germination rate is high, sprout time is unified, simple to operate and better can contact the sprouting device of illumination, to solve the shortcoming existing for above-mentioned prior art.
Utility model content
The purpose of this utility model is to provide a kind of can completely cut off extraneous mushroom, germination rate is high, sprout time is unified, simple to operate and better can contact the sprouting device of illumination.
For achieving the above object, the utility model provides a kind of aseptic coil paper method and sprouts device, seed-bearing aseptic coil paper is filled for carrying, wherein, described aseptic coil paper method is sprouted device and is comprised casing, case lid and at least one clamping plate, described casing is the hollow structure of printing opacity, described hollow structure is formed sprouts chamber, described case lid is on dismountable opening being connected to described casing and seals described sprouting chamber, described case lid offers at least one air-vent, aseptic ventilated membrane is provided with in described air-vent, described clamping plate are arranged in described sprouting chamber, the lower end that described clamping plate offer for aseptic coil paper is inserted and the clamping fixed seam.
Preferably, the bottom of described clamping plate also offers limbers, described limbers with described clamp to stitch be communicated with.
Preferably, described clamping plate being arranged in described sprouting chamber equally.
Preferably, described clamping plate are equally parallel is arranged in described sprouting chamber.
Preferably, the width of described clamping seam is 6mm, and the spacing of adjacent described clamping plate is 25mm.
Preferably, described aseptic coil paper method is sprouted device and is also comprised base, and the bottom of described clamping plate is all fixed on described base.
Preferably, described base and described clamping plate are integral structure.
Preferably, described casing and described case lid are removably connecting of screw-threaded engagement.
Preferably, described casing is barrel-like structure.
Preferably, the light transmittance of described casing is 95%.
Compared with prior art, sprout device due to aseptic coil paper method of the present utility model and there are casing, case lid and clamping plate, casing is the hollow structure of printing opacity, hollow structure forms the sprouting chamber for seed germination, case lid is in being detachably connected with casing, thus sprouting chamber is sealed, clamping plate offer and insert and the clamping fixed seam for aseptic coil paper; When using the utility model to sprout seed, sprout in chamber because clamping plate are arranged at, therefore filling seed-bearing aseptic coil paper is fixed in sprouting chamber by inserting the clamping seam of clamping plate, and sprout the space just defining sealing chamber is placed on the effect of casing at case lid under, thus make seed be in the sprouting chamber of sealing in germination process, effectively make the seed of sprouting and extraneous mushroom completely cut off, improve the germination rate of seed greatly; The aseptic ventilated membrane arranged in the air-vent simultaneously case lid offered, ensure that in Seed Germination and can carry out normal respiration, and this breathing also cannot bring mushroom under the filtration of aseptic ventilated membrane, further ensure that the sprouting chamber residing for seed germination is in gnotobasis; Separately, the casing of translucent construction, ensure that in Seed Germination and can be subject to uniform illumination, makes seed to contact illumination; In summary, aseptic coil paper method of the present utility model sprout device provide aseptic a, ambient stable and the sprouting chamber meeting seed germination environment for seed germination, gnotobasis makes seed germination rate obtain greatly to improve, sealing, the aseptic and sprouting chamber of uniform illumination makes the environment position stable state of all seed germinations, therefore makes seed germination time unification; Therefore, use the aseptic coil paper method of the utility model to sprout device when sprouting seed, can completely cut off extraneous mushroom, germination rate is high, sprout time is unified, simple to operate and better can contact illumination, there is stronger practicality, applicablely extensively to promote the use of.
Accompanying drawing explanation
Fig. 1 is the structural representation that the aseptic coil paper method of the utility model sprouts device.
Fig. 2 is the decomposing schematic representation of Fig. 1.
Fig. 3 is another angle decomposition schematic diagram of Fig. 1.
Fig. 4 is the sectional structure schematic diagram along A-A line in Fig. 1.
Fig. 5 is the sectional structure schematic diagram along B-B line in Fig. 1.
Embodiment
With reference now to accompanying drawing, describe embodiment of the present utility model, element numbers similar in accompanying drawing represents similar element.
As Figure 1-Figure 5, aseptic coil paper method of the present utility model sprouts device 100, seed-bearing aseptic coil paper is filled for carrying, this aseptic coil paper method is sprouted device 100 and is comprised casing 10, case lid 20 and at least one clamping plate 30, the hollow structure of described casing 10 in printing opacity, the casing 10 of translucent construction, ensure that in Seed Germination and can be subject to uniform illumination, seed is made to contact illumination, described hollow structure forms the sprouting chamber 11 for seed germination growth, case lid 20 is on dismountable opening being connected to casing 10 and seals sprouting chamber 11, case lid 20 offers at least one air-vent 21, aseptic ventilated membrane 40 is provided with in air-vent 21, the aseptic ventilated membrane 40 arranged in the air-vent 21 that case lid 20 is offered, ensure that in Seed Germination and can carry out normal respiration, and this breathing also cannot bring mushroom under the filtration of aseptic ventilated membrane 40, clamping plate 30 are arranged to be sprouted in chamber 11, the lower end that clamping plate 30 offer for aseptic coil paper is inserted and the clamping fixed seam 31, the lower end of aseptic coil paper is inserted in clamping seam 31 and is fixed, thus make the upper end of aseptic coil paper just protrude out clamping plate 30, to place seed in the gap of aseptic coil paper, when using the utility model to sprout seed, sprout in chamber 11 because clamping plate 30 are arranged at, therefore filling seed-bearing aseptic coil paper is fixed in sprouting chamber 11 by inserting the clamping seam 31 of clamping plate 30, and sprout the space just defining sealing chamber 11 is placed on the effect of casing 10 at case lid 20 under, thus make seed be in the sprouting chamber 11 of sealing in germination process, effectively make the seed of sprouting and extraneous mushroom completely cut off, improve the germination rate of seed greatly, aseptic ventilated membrane 40 simultaneously on case lid 20, further ensure that sprouting chamber 11 while seed can carry out eupnea is also in gnotobasis, the casing 10 of translucent construction ensure that in Seed Germination and can be subject to uniform illumination, therefore use the aseptic coil paper method of the utility model to sprout device 100 pairs of seeds when sprouting, can completely cut off extraneous mushroom, germination rate is high, sprout time is unified, simple to operate and better can contact illumination, there is stronger practicality, applicablely extensively to promote the use of.Below continue composition graphs 1-Fig. 5 (wherein, in Fig. 1 A-A analyse and observe direction and B-B to cut open direction be along orthogonal two cross sections of casing length direction) to sprout device 100 to aseptic coil paper method of the present utility model and be described in further detail:
It should be noted that, six clamping plate 30 are provided with in Fig. 1-embodiment illustrated in fig. 5, certain described clamping plate 30 also can be set to two, three, four, five, seven, eight etc., under the prerequisite of the technical scheme that the volume cost those skilled in the art of described clamping plate 30 provide in the application, according to the demand of actual conditions, can make one's options and determine without the need to any creative work, no longer describe in detail at this.
Below continue shown in composition graphs 1-Fig. 5, the step utilizing aseptic coil paper method sprouting device of the present utility model 100 pairs of seeds to sprout is briefly explained: aseptic filter paper is rolled into web-like along long limit by (1) before use, it is made to form aseptic coil paper, the method making aseptic coil paper of aseptic filter paper belongs to technology well-known to those skilled in the art, repeats no more; (2) inserted the lower end of aseptic coil paper in the clamping seam 31 of clamping plate 30, the upper end of aseptic coil paper then protrudes out clamping plate 30, and aseptic coil paper is fixed on clamping plate 30 by clamping seam 31; (3) case lid 20 is connected on casing 10, then puts into the sterilizing of high-temperature sterilization pot, after sterilizing in constant temperature oven dry for standby; (4) coil paper method that is aseptic for the utility model of oven dry being sprouted device 100 places on aseptic operating platform, takes off case lid 20 and takes out the good seed of Aseptic sterilisation, sandwiching in the gap of aseptic coil paper with aseptic nipper by one one, seed; (5) in casing 10, appropriate sterile water is poured into; (6) finally again covered on casing 10 by case lid 20, the culturing room put under illumination cultivates, and treats that within three to five days, namely observing young shoot by the casing 10 of translucent construction grows; (7) after completing the sprouting of seed, take out the aseptic coil paper that growth has young shoot, the aseptic coil paper method of cleaning the utility model sprouts device 100, waits for next time and reuses, and makes the utility model aseptic coil paper method sprout device 100 reusable.It should be noted that, the method of operating of above-mentioned steps and sequencing, the technical scheme that those skilled in the art provide according to the application, under the demand of actual conditions, adjustment and the change in multiple applicable practical service environment and place can be carried out, above-mentioned provided concrete steps are only as using aseptic coil paper method of the present utility model to sprout the preference of the operation that device 100 pairs of seeds are sprouted, the interest field of the utility model can not be limited with this, equivalent variations done according to this, still belongs to the scope that the utility model is contained.
As Figure 2-Figure 5, preferably, the bottom of described clamping plate 30 also offers limbers 32, and described limbers 32 is communicated with described clamping seam 31; Stitch 31 due to described limbers 32 with the clamping of fixing aseptic coil paper to be connected, thus make after pour water in casing 10, water is made to enter into clamping seam 31 by limbers 32 on the one hand, thus can absorb by aseptic coil paper for seed germination used, further improve seed germination rate, on the other hand, the water level that the clamping of each clamping plate 30 is stitched in 31 is all positioned at sustained height, thus each clamping plate the water tariff collection that can draw of the aseptic coil paper fixed consistent, further ensure that the unification of seed germination time.
Continue shown in composition graphs 2-Fig. 5, preferably, described aseptic coil paper method is sprouted device 100 and is also comprised base 33, and the bottom of described clamping plate 30 is all fixed on described base 33; By described base 33, whole clamping plate 30 are carried out unifying to fix, when making clamping plate 30 to be positioned in the sprouting chamber 11 of casing 10, more convenient to operate and quick.
Continue shown in composition graphs 2-Fig. 5, preferably, described base 33 and described clamping plate 30 are in integral structure.
As shown in figs 2-4, preferably, described clamping plate 30 equally be arranged in described sprouting chamber 11; Equally spaced clamping plate 30 are set, more utilize and clamping plate 30 are positioned in the sprouting chamber 11 of casing 10, and utilize operation and observe.
Continue shown in composition graphs 2-Fig. 4, preferably, described clamping plate 30 are equally parallel to be arranged in described sprouting chamber 11.
Continue shown in composition graphs 2-Fig. 4, preferably, the width of described clamping seam 31 is 6mm, and the spacing of adjacent described clamping plate 30 is 25mm.
Shown in composition graphs 2-Fig. 5, preferably, described casing 10 and described case lid 20 removably connecting in screw-threaded engagement; Particularly, the opening part outer wall of described casing 10 offers external screw thread 12, the opening part inwall of described case lid 20 offers the internal thread 22 engaged with described external screw thread 12, by the engagement fit of external screw thread 12 with internal thread 22, realize removably connecting of casing 10 and case lid 20, structure is simple, practicality makes, and easy to operate.
Shown in composition graphs 1-Fig. 5, preferably, described casing 10 is in barrel-like structure.
Preferably, the light transmittance of described casing 10 is 95%; Particularly, to be that the PC of high temperature resistant material is made form described casing.
Continue shown in composition graphs 1-Fig. 5, sprout device 100 due to aseptic coil paper method of the present utility model and there are casing 10, case lid 20 and clamping plate 30, the hollow structure of casing 10 in printing opacity, hollow structure forms the sprouting chamber 11 for seed germination, case lid 20 is in being detachably connected with casing 10, thus sprouting chamber 11 is sealed, clamping plate 30 offer and insert and the clamping fixed seam 31 for aseptic coil paper; When using the utility model to sprout seed, sprout in chamber 11 because clamping plate 30 are arranged at, therefore filling seed-bearing aseptic coil paper is fixed in sprouting chamber 11 by inserting the clamping seam 31 of clamping plate 30, and sprout the space just defining sealing chamber 11 is placed on the effect of casing 10 at case lid 20 under, thus make seed be in the sprouting chamber 11 of sealing in germination process, effectively make the seed of sprouting and extraneous mushroom completely cut off, improve the germination rate of seed greatly; The aseptic ventilated membrane 40 arranged in the air-vent 21 simultaneously case lid 20 offered, ensure that in Seed Germination and can carry out normal respiration, and this breathing also cannot bring mushroom under the filtration of aseptic ventilated membrane 40, further ensure that the sprouting chamber 11 residing for seed germination is in gnotobasis; Separately, the casing 10 of translucent construction, ensure that in Seed Germination and can be subject to uniform illumination, makes seed to contact illumination; In summary, aseptic coil paper method of the present utility model sprout device 100 provide aseptic a, ambient stable and the sprouting chamber 11 meeting seed germination environment for seed germination, gnotobasis makes seed germination rate obtain greatly to improve, sealing, the aseptic and sprouting chamber of uniform illumination makes the environment position stable state of all seed germinations, therefore makes seed germination time unification; Therefore, use the aseptic coil paper method of the utility model to sprout device 100 pairs of seeds when sprouting, can completely cut off extraneous mushroom, germination rate is high, sprout time is unified, simple to operate and better can contact illumination, there is stronger practicality, applicablely extensively to promote the use of.
Separately, the principle utilizing aseptic coil paper germination seed involved by the utility model, is well known to those of ordinary skill in the art, is no longer described in detail at this.
Above disclosedly be only preferred embodiment of the present utility model, certainly can not limit the interest field of the utility model with this, therefore according to the equivalent variations that the utility model claim is done, still belong to the scope that the utility model is contained.

Claims (10)

1. an aseptic coil paper method sprouts device, seed-bearing aseptic coil paper is filled for carrying, it is characterized in that: described aseptic coil paper method is sprouted device and comprised casing, case lid and at least one clamping plate, described casing is the hollow structure of printing opacity, described hollow structure is formed sprouts chamber, described case lid is on dismountable opening being connected to described casing and seals described sprouting chamber, described case lid offers at least one air-vent, aseptic ventilated membrane is provided with in described air-vent, described clamping plate are arranged in described sprouting chamber, the lower end that described clamping plate offer for aseptic coil paper is inserted and the clamping fixed seam.
2. aseptic coil paper method as claimed in claim 1 sprouts device, it is characterized in that: the bottom of described clamping plate also offers limbers, described limbers with described clamp to stitch be communicated with.
3. aseptic coil paper method as claimed in claim 1 sprouts device, it is characterized in that: described clamping plate being arranged in described sprouting chamber equally.
4. aseptic coil paper method as claimed in claim 3 sprouts device, it is characterized in that: described clamping plate are equally parallel to be arranged in described sprouting chamber.
5. aseptic coil paper method as claimed in claim 3 sprouts device, it is characterized in that: the width of described clamping seam is 6mm, and the spacing of adjacent described clamping plate is 25mm.
6. aseptic coil paper method as claimed in claim 1 sprouts device, and it is characterized in that: also comprise base, the bottom of described clamping plate is all fixed on described base.
7. aseptic coil paper method as claimed in claim 6 sprouts device, it is characterized in that: described base and described clamping plate are integral structure.
8. aseptic coil paper method as claimed in claim 1 sprouts device, it is characterized in that: described casing and described case lid are removably connecting of screw-threaded engagement.
9. aseptic coil paper method as claimed in claim 1 sprouts device, it is characterized in that: described casing is barrel-like structure.
10. aseptic coil paper method as claimed in claim 1 sprouts device, it is characterized in that: the light transmittance of described casing is 95%.
CN201520861990.8U 2015-10-30 2015-10-30 Device is sprouted to aseptic stock form method Expired - Fee Related CN205105573U (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105917797A (en) * 2016-07-01 2016-09-07 北京农学院 Device for seed roll-paper sprouting test
CN109220760A (en) * 2018-10-24 2019-01-18 杭州师范大学 Holoparasite observation on Growth device
CN109258235A (en) * 2018-10-24 2019-01-25 杭州师范大学 Partial parasite inoculated and cultured device and application method
CN109392685A (en) * 2018-10-24 2019-03-01 杭州师范大学 Multi-functional parasitic plant culture apparatus and application method
CN109430029A (en) * 2018-10-24 2019-03-08 杭州师范大学 Holoparasite sprouts incubator and operating method

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105917797A (en) * 2016-07-01 2016-09-07 北京农学院 Device for seed roll-paper sprouting test
CN105917797B (en) * 2016-07-01 2019-01-08 北京农学院 A kind of device for seed roll paper germination test
CN109220760A (en) * 2018-10-24 2019-01-18 杭州师范大学 Holoparasite observation on Growth device
CN109258235A (en) * 2018-10-24 2019-01-25 杭州师范大学 Partial parasite inoculated and cultured device and application method
CN109392685A (en) * 2018-10-24 2019-03-01 杭州师范大学 Multi-functional parasitic plant culture apparatus and application method
CN109430029A (en) * 2018-10-24 2019-03-08 杭州师范大学 Holoparasite sprouts incubator and operating method
CN109392685B (en) * 2018-10-24 2024-05-14 杭州师范大学 Multifunctional parasitic plant culture device and use method
CN109258235B (en) * 2018-10-24 2024-05-14 杭州师范大学 Semi-parasitic plant inoculation culture device and use method
CN109220760B (en) * 2018-10-24 2024-05-14 杭州师范大学 Full parasitic plant growth observation device
CN109430029B (en) * 2018-10-24 2024-05-14 杭州师范大学 Total parasitic plant germination incubator and operation method

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Granted publication date: 20160330

Termination date: 20161030