CN205088166U - Integrated form polypeptide solid -phase synthesis system - Google Patents
Integrated form polypeptide solid -phase synthesis system Download PDFInfo
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- CN205088166U CN205088166U CN201520876097.2U CN201520876097U CN205088166U CN 205088166 U CN205088166 U CN 205088166U CN 201520876097 U CN201520876097 U CN 201520876097U CN 205088166 U CN205088166 U CN 205088166U
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- phase synthesis
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- polypeptide solid
- conical flask
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 64
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 61
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 61
- 238000010532 solid phase synthesis reaction Methods 0.000 title claims abstract description 56
- 239000007788 liquid Substances 0.000 claims abstract description 72
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 64
- 239000002699 waste material Substances 0.000 claims abstract description 36
- 239000007789 gas Substances 0.000 claims abstract description 14
- 235000019353 potassium silicate Nutrition 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 51
- 239000011521 glass Substances 0.000 claims description 28
- 229910052757 nitrogen Inorganic materials 0.000 claims description 25
- 238000000227 grinding Methods 0.000 claims description 13
- 238000003786 synthesis reaction Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 8
- 238000005086 pumping Methods 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 239000007790 solid phase Substances 0.000 claims description 4
- 239000000376 reactant Substances 0.000 claims description 3
- 229910001873 dinitrogen Inorganic materials 0.000 abstract description 14
- 238000003756 stirring Methods 0.000 abstract description 9
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 13
- 150000001413 amino acids Chemical group 0.000 description 6
- 239000011347 resin Substances 0.000 description 5
- 229920005989 resin Polymers 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000007799 cork Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000010647 peptide synthesis reaction Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 2
- 238000010511 deprotection reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 125000006239 protecting group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102400000050 Oxytocin Human genes 0.000 description 1
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 description 1
- 101800000989 Oxytocin Proteins 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000012351 deprotecting agent Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 description 1
- 229960001723 oxytocin Drugs 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The utility model discloses an integrated form polypeptide solid -phase synthesis system, including polypeptide solid -phase synthesis pipe, erlenmeyer flask, nitrogen gas jar, vacuum pump and two three -way valves, the bottom of polypeptide solid -phase synthesis pipe is connected with the first interface of two three -way valves, the second interface of two three -way valves passes through the gas outlet of nitrogen gas hose connection nitrogen gas jar, is equipped with the air outlet valve on the gas outlet of nitrogen gas jar, the 3rd interface connection of two three -way valves has a liquid glass pipe, goes out liquid glass pipe the top seal downward and erlenmeyer flask and feeds through, the upper portion of erlenmeyer flask is equipped with connection for vacuum pump, and connection for vacuum pump is connected with the vacuum pump through the vacuum tube way. Two three -way valves make the polypeptide solid -phase synthesis pipe can select to communicate nitrogen gas jar or erlenmeyer flask, so only need operate two three -way valves and can select to carry on the nitrogen gas stirring or carry out the waste liquid and collect, need not not carry out plug and connection temporarily. The utility model discloses the functions such as nitrogen gas stirring, negative pressure absorption waste liquid that have integrateed are convenient for carry on the polypeptide solid -phase synthesis reaction.
Description
Technical Field
The utility model relates to a peptide synthesis technical field especially relates to a polypeptide solid phase synthesis system.
Background
Polypeptide synthesis studies have gone over a hundred years of bright history. In the 50 s of the 20 th century, organic chemists synthesized a large number of bioactive polypeptides including oxytocin, insulin and the like, and achieved a lot of results on the polypeptide synthesis method and amino acid protecting groups, which provided experimental and theoretical bases for the emergence of subsequent solid phase synthesis methods.
In 1963, Merrifield proposed a solid phase peptide synthesis method (SPPS) for the first time, and the method became the first choice for peptide synthesis due to its convenient and rapid synthesis, which brought a revolution on peptide organic synthesis and became an independent subject, namely Solid Phase Organic Synthesis (SPOS), and Merrifield consequently won the Nobel prize of chemistry in 1984.
The solid-phase synthesis of polypeptide includes connecting protected amino acid to solid resin, eliminating protecting group, reacting the next protected amino acid with the residue of the first amino acid to form peptide bond, eliminating the protecting group of the second amino acid, and connecting the next amino acid, so that the peptide chain is grown circularly. In solid phase synthesis of polypeptides, solid resins are the necessary reaction substrates.
In solid phase synthesis of polypeptides, it is necessary to control the temperature in a suitable range. The reaction proceeds faster with an appropriate increase in temperature, but the decomposition of the peptide is also accelerated with an increase in temperature. No polypeptide solid phase synthesis device capable of flexibly controlling the reaction temperature in the reaction process exists in the market.
The existing polypeptide solid-phase synthesis device mostly adopts a filter flask to contain waste liquid after reaction, the waste liquid is stored for a certain amount, the reaction is stopped, the filter flask is taken down independently, then the waste liquid is poured out, and most of the waste liquid is volatile and toxic organic solvent. This device is got up the one side and is convenient inadequately, and on the other hand causes easily revealing of chemical hazardous article, does not conform to laboratory safety regulation.
In the existing polypeptide solid phase synthesis device, a cork or a rubber plug is generally used for sealing a filter flask, a glass tube for introducing waste liquid into the filter flask needs to be inserted into and penetrate through the cork or the rubber plug, when the glass tube is inserted into the cork or the rubber plug, the operation needs to be very careful, certain operation skill is needed, and the phenomenon that the glass tube is broken and damaged easily occurs.
The existing polypeptide solid phase automatic synthesizer is expensive, and a polypeptide solid phase synthesis system which is convenient to use, simple in structure and convenient to popularize and apply is required in the market.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple structure, utilize integrated form polypeptide solid phase synthesis system of nitrogen gas stirring.
In order to achieve the purpose, the integrated polypeptide solid phase synthesis system of the utility model comprises a polypeptide solid phase synthesis pipe, a conical flask, a nitrogen tank, a vacuum pump and a two-position three-way valve which enables the polypeptide solid phase synthesis pipe to be selectively communicated with the nitrogen tank or the conical flask;
the top of the polypeptide solid phase synthesis tube is open, and the bottom of the polypeptide solid phase synthesis tube is provided with a sand core filter core for supporting reactants; the bottom end of the polypeptide solid phase synthesis pipe is connected with a first interface of the two-position three-way valve;
a second interface of the two-position three-way valve is connected with the gas outlet of the nitrogen tank through a nitrogen hose, and a gas outlet valve is arranged on the gas outlet of the nitrogen tank;
a third interface of the two-position three-way valve is fixedly connected with a liquid glass outlet pipe, and the liquid glass outlet pipe is downwards communicated with the top of the conical flask in a sealing way; the upper part of the conical flask is provided with a vacuumizing interface, and the vacuumizing interface is connected with a vacuum pump for vacuumizing the conical flask through a vacuumizing pipeline.
The polypeptide solid phase synthesis pipe is sleeved with a water jacket, the bottom of one side of the water jacket is connected with a water inlet pipe, and the top of the other side of the water jacket is connected with a water outlet pipe; the water inlet pipe is provided with a water pump for pumping water from a water source to the water jacket;
the top of the conical flask is provided with a grinding opening for feeding liquid, the grinding opening is in a conical shape with a large upper part and a small lower part, and the inner surface of the grinding opening is a frosted surface; the bottom end of the liquid outlet glass tube is integrally provided with a glass plug, the middle part of the glass plug is provided with a central hole which vertically penetrates through the glass plug, and the central hole is communicated with the liquid outlet glass tube; the outer surface of the glass plug is a frosted surface and is a conical surface matched with the grinding opening, and the glass plug is inserted in the grinding opening and is in sealing fit with the inner surface of the grinding opening;
a waste liquid cylinder is arranged on one side of the conical flask, a liquid outlet is formed in the bottom of the conical flask, a liquid outlet hose is connected with the liquid outlet, and the free end of the liquid outlet hose is communicated with the waste liquid cylinder; a clip or a liquid outlet valve is arranged on the liquid outlet hose; the top of the waste liquid tank is lower than the bottom of the conical flask.
The utility model discloses a two three-way valves make polypeptide solid phase synthesis pipe can select intercommunication nitrogen gas jar or erlenmeyer flask, only need operate two three-way valves like this and can select whether carry out the nitrogen gas stirring or carry out the waste liquid and collect, need not carry out plug and connection temporarily, very facilitate the use. The utility model discloses the top of well erlenmeyer flask is connected with the vacuum pump, can form the negative pressure in the erlenmeyer flask very conveniently to make the intraductal waste liquid of polypeptide solid phase synthesis penetrate the psammitolite filter core and get into the erlenmeyer flask under the effect of atmospheric pressure in, made things convenient for the collection of waste liquid. In a word, the utility model discloses integrated functions such as nitrogen gas stirring, negative pressure absorb waste liquid, very conveniently carry out polypeptide solid phase synthesis reaction.
The setting of gas outlet valve can control the break-make of nitrogen gas on the one hand, and on the other hand can control the intensity of the stirring of nitrogen gas as required thereby the degree of opening of gas outlet valve, and the staff can be with the intensity control of nitrogen gas stirring at the most suitable degree.
The temperature of a water source is controlled according to the synthesis requirement, and the polypeptide solid phase synthesis tube can be always controlled at the required temperature through a water jacket, so that a good temperature condition is provided for the synthesis reaction.
The erlenmeyer flask top is equipped with the mill mouth, and the third interface of two three-way valves is through going out liquid glass pipe and glass stopper connection erlenmeyer flask, and the surface of glass stopper and the internal surface of erlenmeyer flask are the toper frosting of mutual adaptation, therefore the plug-in connection of being convenient for very much, and the back of linking together can form sealed condition naturally through the cooperation of frosting between mill mouth and the glass stopper. The arrangement not only facilitates the connection, but also avoids the phenomenon that the glass tube is broken and damaged when the glass tube is inserted into the cork or the rubber plug.
The liquid outlet of the conical flask is connected with a liquid outlet hose, and the free end of the liquid outlet hose is communicated with the waste liquid cylinder. By adopting the arrangement, when more waste liquid is accumulated in the conical flask and needs to be cleaned, the conical flask is not required to be separated from other equipment, but only the clamp is required to be taken down or the liquid outlet valve is required to be opened, so that the waste liquid can flow into the waste liquid cylinder, the leakage of volatile and toxic chemical substances can be prevented, the contact between an operator and an experimental reagent is effectively reduced, and the use is further facilitated.
Finally, the utility model discloses simple structure, convenient to use is convenient for make and connect the use.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic diagram of a two-position three-way valve;
fig. 3 is a schematic structural view of a conical flask.
Detailed Description
As shown in FIG. 1, FIG. 2 and FIG. 3, the integrated polypeptide solid phase synthesis system of the present invention comprises a polypeptide solid phase synthesis tube 1, a conical flask 2, a nitrogen tank 3, a waste liquid tank 4, a vacuum pump 5 and a two-position three-way valve 6 for connecting the polypeptide solid phase synthesis tube 1 to the nitrogen tank 3 or the conical flask 2.
The top of the polypeptide solid phase synthesis tube 1 is open, and the bottom is provided with a sand core filter core 7 for supporting reactants; the bottom end of the polypeptide solid phase synthesis pipe 1 below the sand core filter element 7 is connected with a first connector 8 of the two-position three-way valve 6.
And a second connector 9 of the two-position three-way valve 6 is connected with a gas outlet 12 of the nitrogen tank 3 through a nitrogen hose 11, and a gas outlet valve 13 is arranged on the gas outlet 12 of the nitrogen tank 3.
A third interface 10 of the two-position three-way valve 6 is fixedly connected with a liquid glass outlet pipe 14, and the liquid glass outlet pipe 14 is downwards communicated with the top of the conical flask 2 in a sealing way; the upper part of the conical flask 2 is provided with a vacuum-pumping interface 15, and the vacuum-pumping interface 15 is connected with the vacuum pump 5 for vacuumizing the conical flask 2 through a vacuum-pumping pipeline 16.
A water jacket 17 is sleeved outside the polypeptide solid phase synthesis tube 1, the bottom of one side of the water jacket 17 is connected with a water inlet pipe 18, and the top of the other side of the water jacket 17 is connected with a water outlet pipe 19; the water inlet pipe 18 is provided with a water pump 20 for pumping water from a water source to the water jacket 17;
the liquid glass outlet pipe 14 is communicated with the top of the conical flask 2 in a sealing way through the following structures:
the top of the conical flask 2 is provided with a ground opening 21 for feeding liquid, the ground opening 21 is in a conical shape with a large upper part and a small lower part, and the inner surface of the ground opening is a frosted surface; a glass plug 22 is integrally arranged at the bottom end of the liquid glass outlet pipe 14, a central hole 23 which vertically penetrates through the glass plug 22 is formed in the middle of the glass plug 22, and the central hole 23 is communicated with the liquid glass outlet pipe 14; the outer surface of the glass plug 22 is a frosted surface and is a conical surface with a large upper part and a small lower part matched with the frosted opening 21, and the glass plug 22 is inserted in the frosted opening 21 and is in sealing fit with the inner surface of the frosted opening 21.
The waste liquid cylinder 4 is arranged on one side of the conical flask 2, the liquid outlet 24 is arranged at the bottom of the conical flask 2, the liquid outlet 24 is connected with a liquid outlet hose 25, and the free end of the liquid outlet hose 25 is introduced into the waste liquid cylinder 4; a clip or a liquid outlet valve 26 is arranged on the liquid outlet hose 25; the top of the waste liquid cylinder 4 is lower than the bottom of the conical flask 2, so that the waste liquid in the conical flask 2 can flow out smoothly.
Wherein,the polypeptide solid phase synthesis pipe 1, the two-position three-way valve 6, the nitrogen tank 3, the vacuum pump 5, the water pump 20, the sand core filter element 7 and the like are all conventional components,it is composed ofThe detailed structure is not described in detail。
When the circulating water bath pipeline is used, the water temperature of a water source is adjusted to be required, the water inlet pipe 18 is connected with the water outlet of the water source, and the water outlet pipe 19 is connected with the water inlet of the water source, so that a circulating water bath pipeline is formed. Of course, the water in the outlet pipe 19 may also flow into the sewer as waste water. The water pump 20 is turned on, and water in the water source is pumped to the bottom of one side of the water jacket 17, passes through the water jacket 17 and then flows out from the top of the other side of the water jacket 17. The temperature of the water source is controlled according to the synthesis requirement, the polypeptide solid phase synthesis tube 1 can be always controlled at the required temperature, and good temperature conditions are provided for the synthesis reaction.
Resin (solid state) is put into the polypeptide solid phase synthesis tube 1 from the top opening of the tube, and the resin is supported by the sand core filter element 7. Then, the solvent in which the activating agent or the deprotecting agent is dissolved is poured into the polypeptide solid-phase synthesis tube 1, thereby carrying out the synthesis reaction. And controlling the working state of the two-position three-way valve 6 to ensure that the first interface 8 is communicated with the second interface 9, and further ensuring that the polypeptide solid phase synthesis pipe 1 is communicated with the nitrogen tank 3. And opening an air outlet valve 13 at the air outlet 12 of the nitrogen tank 3 to ensure that nitrogen enters the polypeptide solid phase synthesis pipe 1 from the bottom end thereof and then upwards enters the solvent through the sand core filter element 7. The nitrogen is continuously introduced into the solvent, so that rising nitrogen bubbles are continuously generated in the solvent, and a sufficient stirring effect is achieved. The opening degree of the air outlet valve 13 is controlled, so that the stirring intensity of the nitrogen can be controlled.
And after the reaction, closing an air outlet valve 13 of the nitrogen tank 3, and adjusting the working state of the two-position three-way valve 6 to ensure that the first connector 8 is communicated with the third connector 10, so that the polypeptide solid-phase synthesis pipe 1 is communicated with the conical flask 2. Opening a vacuum pump 5 to vacuumize the conical flask 2; under the action of negative pressure, the reacted solvent waste liquid enters the conical flask 2 through the sand core filter element 7, the two-position three-way valve 6 and the grinding opening 21 and is stored at the bottom of the conical flask 2. The waste liquid can be taken off (the glass stopper 22 is extracted) and then the waste liquid is poured into the waste liquid cylinder 4 manually after the waste liquid is accumulated in the conical flask 2 more. Under the condition that the liquid outlet hose 25 is arranged, the waste liquid can naturally flow into the waste liquid cylinder 4 under the action of gravity only by taking down the clamp from the liquid outlet hose 25 or opening the liquid outlet valve 26, and the use is very convenient. After the waste liquid flows out, the clamp is clamped on the liquid outlet hose 25 or the liquid outlet valve 26 is closed.
In the solid phase synthesis reaction of polypeptide, an activating agent and a deprotection agent are required to be alternately used as a reaction solution to participate in the reaction. When the reaction solution is replaced, the gas outlet valve 13 of the nitrogen tank 3 is closed, the working state of the two-position three-way valve 6 is adjusted, the first connector 8 is communicated with the third connector 10, and the polypeptide solid phase synthesis tube 1 is communicated with the conical flask 2. Then starting a vacuum pump 5, pumping the waste liquid in the polypeptide solid phase synthesis tube 1 into the conical flask 2, then adding a washing liquid into the polypeptide solid phase synthesis tube 1 for washing, and sucking the washed washing liquid into the conical flask 2 under the negative pressure generated by the vacuum pump 5. After cleaning, the vacuum pump 5 is closed, and the two-position three-way valve 6 is adjusted again to communicate the polypeptide solid phase synthesis pipe 1 with the nitrogen tank 3; the reaction solution was added to the polypeptide solid-phase synthesis tube 1, and the reaction at the next stage was started. Change activator and deprotection agent reaction liquid in turn and react, until the reaction finishes, after polypeptide solid phase synthesis pipe 1 is taken out to the waste liquid, can take out the resin with reaction product (polypeptide) from polypeptide solid phase synthesis intraductal 1, accomplish the utility model discloses a whole functions.
The above embodiments are only used for illustrating but not limiting the technical solutions of the present invention, and although the present invention is described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: the present invention may be modified or substituted with equivalents without departing from the spirit and scope of the invention, which should be construed as being limited only by the claims.
Claims (2)
1. Integrated polypeptide solid phase synthesis system, its characterized in that: comprises a polypeptide solid phase synthesis pipe, a conical flask, a nitrogen tank, a vacuum pump and a two-position three-way valve which enables the polypeptide solid phase synthesis pipe to be selectively communicated with the nitrogen tank or the conical flask;
the top of the polypeptide solid phase synthesis tube is open, and the bottom of the polypeptide solid phase synthesis tube is provided with a sand core filter core for supporting reactants; the bottom end of the polypeptide solid phase synthesis pipe is connected with a first interface of the two-position three-way valve;
a second interface of the two-position three-way valve is connected with the gas outlet of the nitrogen tank through a nitrogen hose, and a gas outlet valve is arranged on the gas outlet of the nitrogen tank;
a third interface of the two-position three-way valve is fixedly connected with a liquid glass outlet pipe, and the liquid glass outlet pipe is downwards communicated with the top of the conical flask in a sealing way; the upper part of the conical flask is provided with a vacuumizing interface, and the vacuumizing interface is connected with a vacuum pump for vacuumizing the conical flask through a vacuumizing pipeline.
2. The integrated solid phase polypeptide synthesis system of claim 1, wherein:
the polypeptide solid phase synthesis pipe is sleeved with a water jacket, the bottom of one side of the water jacket is connected with a water inlet pipe, and the top of the other side of the water jacket is connected with a water outlet pipe; the water inlet pipe is provided with a water pump for pumping water from a water source to the water jacket;
the top of the conical flask is provided with a grinding opening for feeding liquid, the grinding opening is in a conical shape with a large upper part and a small lower part, and the inner surface of the grinding opening is a frosted surface; the bottom end of the liquid outlet glass tube is integrally provided with a glass plug, the middle part of the glass plug is provided with a central hole which vertically penetrates through the glass plug, and the central hole is communicated with the liquid outlet glass tube; the outer surface of the glass plug is a frosted surface and is a conical surface matched with the grinding opening, and the glass plug is inserted in the grinding opening and is in sealing fit with the inner surface of the grinding opening;
a waste liquid cylinder is arranged on one side of the conical flask, a liquid outlet is formed in the bottom of the conical flask, a liquid outlet hose is connected with the liquid outlet, and the free end of the liquid outlet hose is communicated with the waste liquid cylinder; a clip or a liquid outlet valve is arranged on the liquid outlet hose; the top of the waste liquid tank is lower than the bottom of the conical flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520876097.2U CN205088166U (en) | 2015-11-06 | 2015-11-06 | Integrated form polypeptide solid -phase synthesis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520876097.2U CN205088166U (en) | 2015-11-06 | 2015-11-06 | Integrated form polypeptide solid -phase synthesis system |
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| Publication Number | Publication Date |
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| CN205088166U true CN205088166U (en) | 2016-03-16 |
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| CN201520876097.2U Expired - Fee Related CN205088166U (en) | 2015-11-06 | 2015-11-06 | Integrated form polypeptide solid -phase synthesis system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986916A (en) * | 2017-03-20 | 2017-07-28 | 珠海百世瑞生命科技有限公司 | Full automatic high efficiency solid phase peptide synthesizer and application method |
| CN108676057A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Solid phase peptide synthssis device |
-
2015
- 2015-11-06 CN CN201520876097.2U patent/CN205088166U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986916A (en) * | 2017-03-20 | 2017-07-28 | 珠海百世瑞生命科技有限公司 | Full automatic high efficiency solid phase peptide synthesizer and application method |
| CN106986916B (en) * | 2017-03-20 | 2023-10-13 | 珠海百世瑞生命科技有限公司 | Full-automatic high-efficiency solid-phase polypeptide synthesizer and use method thereof |
| CN108676057A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Solid phase peptide synthssis device |
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