CN206232638U - Improve the Solid-phase synthesis peptides pipe of reactant utilization rate - Google Patents
Improve the Solid-phase synthesis peptides pipe of reactant utilization rate Download PDFInfo
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- CN206232638U CN206232638U CN201621317575.7U CN201621317575U CN206232638U CN 206232638 U CN206232638 U CN 206232638U CN 201621317575 U CN201621317575 U CN 201621317575U CN 206232638 U CN206232638 U CN 206232638U
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- pipe
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- spherical body
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Abstract
The utility model discloses a kind of Solid-phase synthesis peptides pipe for improving reactant utilization rate, including spherical body, spherical body top is connected with push pipe, and push pipe top is connected with breather pipe, and breather pipe is provided with two-port valve；Spherical tube wall top has connected up feed pipe, and feed pipe top is connected with charging spiral cover；Spherical body bottom is connected with straight length, and core filter core is provided with straight length；The bottom of straight length is connected with drain pipe, and drain pipe is provided with two-position three-way valve, and two-position three-way valve has top interface, bottom interface and sidepiece interface, and bottom interface is connected with discharging tube, and discharging tube is used to connect the waste fluid container for containing waste liquid.The utility model can improve the utilization rate of raw material, save reactant, improves the successful probability of reaction, do not influence to dip reactant using glass bar and detected.When there is nitrogen shakiness phenomenon, reactant will not be rushed out Solid-phase synthesis peptides pipe.
Description
Technical field
The utility model is related to peptide synthesis technology field, more particularly to Solid-phase synthesis peptides technology.
Background technology
The brilliant course that Peptide systhesis research has been passed by more than 100 years.The 1950's, organic chemists close
Into substantial amounts of biologically active polypeptide, including oxytocins, insulin etc., while in polypeptide synthesis method and amino acid protecting groups
Many achievements are also achieved above, and this provides experiment and theoretical foundation for the appearance of later solid phase synthesis process.
1963, Merrifield proposed solid-phase peptide synthesis (SPPS) first, once occur due to its synthesize
Prefered method that is convenient, rapid and turning into Peptide systhesis, brings the revolution in polypeptide organic synthesis, and become one
Therefore independent subject --- Solid-phase organic synthesis (SPOS), Merrifield has also won the Nobel chemistry Prize of 1984.
In Solid-phase synthesis peptides, Solid-phase synthesis peptides pipe is frequently used, resin and anti-is added in Solid-phase synthesis peptides pipe
Answer liquid（Reaction solution is the solvent for dissolving in activator or deprotection agent）Carry out synthetic reaction.
Solid-phase synthesis peptides are that protected amino acid is connected on solid resin, slough blocking group, then will be next
The residue of protected amino acid and first amino acid reacts and forms peptide bond, then sloughs second blocking group of amino acid,
Next amino acid is reconnected, so circulation goes down to make peptide chain to increase.In Solid-phase synthesis peptides, solid resin is to have to use for
Reaction substrate.
Because Solid-phase synthesis peptides are carried out step by step, therefore before next step reaction is carried out, it is necessary to detect
Once the reaction result of previous step, if previous step is reacted successfully, proceeds next step reaction；If previous step is reacted not
Success, then carry out next step reaction just completely nonsensical, it is necessary to restart reaction.Between two-step reaction, detection upper
, it is necessary to stretch into Solid-phase synthesis peptides pipe with glass bar during step reaction result, dip reactant and detected.
Existing Solid-phase synthesis peptides pipe is all to be provided with the opening for being put into or taking out reactant at top, glass during detection
Glass rod stretches into spherical body from open top straight down, and dips reactant.This mode, it is impossible to avoid nitrogen described below
The gas phenomenon that caused reactant sprays when unstable.If Solid-phase synthesis peptides pipe sidewall opening and for picking and placeing reaction
Thing, and the device for ventilating and stopping solids is set at the top of Solid-phase synthesis peptides pipe, reactant can be avoided to spray, but by
Cylindrical long tube is in traditional Solid-phase synthesis peptides pipe, therefore cannot stretch into polypeptide after glass bar is stretched into from sidewall opening consolidate
It is combined to the bottom of pipe, it is impossible to dip reactant, it is impossible to complete detection work.
Nitrogen shakiness phenomenon refer to：When carrying out synthetic reaction, in order to accelerate reaction, through the mode frequently with nitrogen gas stirring,
Accelerate the synthetic reaction of resin and reaction solution.It sometimes appear that the unstable phenomenon of nitrogen during nitrogen gas stirring, i.e., into polypeptide
The nitrogen pressure of synthesis in solid state pipe is unstable, causes reactant（Resin or reaction solution）By Solid-phase synthesis peptides pipe open top
The phenomenon of ejection, what influence was reacted is normally carried out.Even if nitrogen is unstable, reactant is not sprayed onto outside from composite tube,
Have partial reaction thing to be sprayed onto on the tube wall of composite tube, reactant utilization rate declines, and even results in test failure.
Utility model content
The purpose of this utility model is to provide one kind to prevent the nitrogen shakiness from causing reactant to spray, and can be carried again
The utilization rate of reactant high, and do not influence to dip the Solid-phase synthesis peptides of the raising reactant utilization rate of reactant during detection
Pipe.
To achieve the above object, the Solid-phase synthesis peptides pipe for improving reactant utilization rate of the present utility model includes bulb tube
Body, spherical body top is connected with push pipe, and push pipe top is connected with breather pipe, and breather pipe is provided with two-port valve, breather pipe socket
There is the rubber tube for connecting high-pressure air source；
Spherical tube wall top has connected up feed pipe, and feed pipe top is threaded with charging spiral cover；Bulb tube
Body bottom is connected with straight length, and core filter core is provided with straight length；The center line of feed pipe passes through the core filter core；
The bottom of straight length is connected with drain pipe, and drain pipe is provided with two-position three-way valve, and two-position three-way valve connects with top
Mouth, bottom interface and sidepiece interface, top interface are connected with drain pipe, and sidepiece interface is used to connect nitrogen air induction hose, bottom
Portion's interface is connected with discharging tube, and discharging tube is used to connect the waste fluid container for containing waste liquid.
Core plate is provided with the push pipe, the vent valve uses plug valve.
The ventilation bottom of the tube is connected with grinding port plug, and grinding port plug is adapted with push pipe and inserts push pipe top.
When reactant and partial reaction liquid to be rushed nitrogen shakiness phenomenon the madial wall to spherical body, stop nitrogen and stir
Mix, using syringe or similar devices, by breather pipe and push pipe to appropriate reaction solution is injected on core plate, then by rubber sleeve
It is connected on breather pipe, the other end of rubber tube is connected on air pump or on high pressure gas holder, so that top intraductal atmospheric pressure is raised,
And then the reaction solution on core plate is passed down through core plate into spherical body under the influence of air pressure.Due to anti-during this
Liquid is answered to flow slowly over core plate, therefore reaction solution can flow downward along the side wall perimeter of spherical body, so as to will be attached to
Reactant on spherical tube wall sweeps away, and falls again on core filter core, continues course of reaction.Which improves this practicality
The new utilization rate to raw material, has not only saved reactant, and improve the successful probability of reaction.
In a word, the utility model can improve the utilization rate of raw material, save reactant, improves the successful probability of reaction, together
When do not influence to dip reactant using glass bar and detected.When there is nitrogen shakiness phenomenon, reactant can by core plate and
Charging spiral cover is blocked, and will not be rushed out Solid-phase synthesis peptides pipe.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Specific embodiment
As shown in figure 1, the Solid-phase synthesis peptides pipe for improving reactant utilization rate of the present utility model includes spherical body 1,
The top of spherical body 1 is connected with push pipe 2, and the top of push pipe 2 is connected with breather pipe 3（Push pipe 2 is connected with breather pipe 3）, breather pipe 3
Two-port valve 4 is provided with, breather pipe 3 is socketed with for connecting high-pressure air source（Such as high pressure gas holder source of the gas）Rubber tube；High pressure gas
Source and rubber tube are prior art, not shown.The bottom of the breather pipe 3 is connected with grinding port plug 17, and grinding port plug 17 is mutually fitted with push pipe 2
With and insert the top of push pipe 2.
The side wall upper part of spherical body 1 has connected up feed pipe 5, and the top of feed pipe 5 is threaded with charging spiral cover 6；Ball
The bottom of shape body 1 is connected with straight length 7, straight length 7 and is provided with core filter core 8；The center line of feed pipe 5 is filtered by the core
Core 8；To ensure that glass bar can be extended at core filter core 8 by feed pipe 5, so as to dip reactant.
The bottom of straight length 7 is connected with drain pipe 9, and drain pipe 9 is provided with two-position three-way valve 10, and two-position three-way valve 10 has
Top interface 11, bottom interface 12 and sidepiece interface 13, top interface 11 are connected with drain pipe 9, and sidepiece interface 13 is used to connect
Nitrogen air induction hose 14 is connect to realize nitrogen gas stirring in being reacted in Solid-phase synthesis peptides, bottom interface 12 is connected with discharging tube, arranged
Liquid pipe is used to connect the waste fluid container for containing waste liquid（Such as conical flask or test tube container；When waste fluid container uses conical flask,
Discharging tube bottom is provided for the ground 16 being adapted in the bottleneck of conical flask）.
Core plate 15 is provided with the push pipe 2.Core plate 15 is made with core filter core 8 of same material, is existing dress
Put, can by gas and liquid, but can be to block solid to pass through.The vent valve uses plug valve.
Core plate 15 can play a part of drainage, make the reaction solution above it in the presence of malleation along the week of push pipe 2
While flow down, and then top perimeter along spherical body 1 flows downward, so as to will be attached to the reactant on the inwall of spherical body 1
Take back the top of core filter core 8.
During normal reaction, rubber tube is removed from push pipe 2, open vent valve, reactant is put into from feed pipe 5（Resin）
And reaction solution, charging spiral cover 6 is then screwed on, reactant is rushed out by feed pipe 5 when preventing nitrogen unstable.Reactant and reaction
Liquid is reacted on core filter core 8（The hole of core filter core 8 is very small, and reaction solution could be fast only in the presence of negative pressure
Speed is flowed down, and reaction solution can only very slowly pass through core filter core 8 under atmospheric pressure state）.
Nitrogen air induction hose 14 is set to connect external high pressure nitrogen tank, high pressure nitrogen tank is existing apparatus, not shown.In nitrogen
During gas agitating, the top interface 11 of two-position three-way valve 10 is connected with sidepiece interface 13, and nitrogen passes through the He of nitrogen air induction hose 14
Two-position three-way valve 10 enters straight length 7, by producing the stirring action of class pseudo boiling after core filter core 8 to reaction solution, promotes anti-
The carrying out answered.
Solid-phase synthesis peptides are carried out step by step, before next step reaction is carried out, feed pipe 5 are passed through with glass bar
Solid-phase synthesis peptides pipe is stretched into, reactant is dipped and is detected, it is anti-once under proceeding if previous step is reacted successfully
Should.When reaction carries out nitrogen gas stirring in carrying out, vent valve is opened, the gas in spherical body 1 is allowed while nitrogen is passed through
Diffused away from vent valve, it is ensured that nitrogen gas stirring is normally carried out, and kept in spherical body 1 in atmospheric pressure state.
When reactant and partial reaction liquid to be rushed nitrogen shakiness phenomenon the madial wall to spherical body 1, stop nitrogen and stir
Mix；Using syringe or similar devices, by breather pipe 3 and push pipe 2 to appropriate reaction solution is injected on core plate 15, then by rubber
Pipe sleeve is connected on the breather pipe 3 of vent valve, and the other end of rubber tube is connected on air pump or on high pressure gas holder, so that top
Air pressure is raised in pipe 2, and then it is spherical the reaction solution on core plate 15 is passed down through the entrance of core plate 15 under the influence of air pressure
Body 1.Because reaction solution flows slowly over core plate 15 during this, therefore reaction solution can be along the side wall week of spherical body 1
While flowing downward, swept away so as to will be attached to the reactant on the side wall of spherical body 1, fallen again on core filter core 8, continue anti-
Answer process.Which improves the utility model to the utilization rate of raw material, reactant is not only saved, and improve and react into
The probability of work(.After reactant is swept away, rubber tube can be removed from the breather pipe 3 of vent valve again.
Reaction solution turns into waste liquid, it is necessary to when discharging waste liquid after the completion of reaction, make the top interface 11 of two-position three-way valve 10 with
Bottom interface 12 is connected, and uses vavuum pump container to drain pipe 9 or below（Such as conical flask）Vacuumized, negative pressure by
Drain pipe is transferred at core filter core 8, under waste liquid is inhaled.
The utility model is no as prior art, by feed pipe 5（Mouthful）The positive top of spherical body 1 is arranged on, is
Because feed pipe 5 needs closing when reaction is carried out, and is needed by push pipe 2 when rinsing 1 side wall of spherical body.If feed pipe 5
The positive top of spherical body 1 of Solid-phase synthesis peptides pipe is arranged on, then means that push pipe 2 needs to be arranged on the side wall of spherical body 1, this
Sample, the liquid of spherical body 1 is flowed into from push pipe 2, cannot as be arranged on positive top it is the same along at the top of spherical body 1 to bulb tube
The peripheral sidewalls uniform flow of body 1, so as to be rinsed to the whole side wall of spherical body 1, but can only connect side to push pipe 2
The madial wall of spherical body 1 be rinsed.
If spherical body 1 is not spherical body 1, but the cylindrical spherical body 1 similar with test tube, then spherical body
After 1 side wall upper part connects up feed pipe 5, the axis of the feed pipe 5 substantially will not be by core filter core 8.Using ball
Shape body 1, is highly convenient for the setting of feed pipe 5, is to connect feed pipe 5 and allow glass bar to pass through in the side wall of spherical body 1
Feed pipe 5 extends core filter core 8 and provides basis.
Above example is only used to illustrative and not limiting the technical solution of the utility model, although with reference to above-described embodiment pair
The utility model has been described in detail, it will be understood by those within the art that：The utility model can still be entered
Row modification or equivalent, without deviating from any modification or partial replacement of spirit and scope of the present utility model, it all should
Cover in the middle of right of the present utility model.
Claims (1)
1. the Solid-phase synthesis peptides pipe of reactant utilization rate is improved, it is characterised in that：Including spherical body, spherical body top connects
Push pipe is connected to, push pipe top is connected with breather pipe, and breather pipe is provided with two-port valve, and breather pipe is socketed with for connecting high-pressure air source
Rubber tube；
Spherical tube wall top has connected up feed pipe, and feed pipe top is threaded with charging spiral cover；Spherical body bottom
End is connected with straight length, and core filter core is provided with straight length；The center line of feed pipe passes through the core filter core；
The bottom of straight length is connected with drain pipe, and drain pipe is provided with two-position three-way valve, and two-position three-way valve has top interface, bottom
Portion's interface and sidepiece interface, top interface are connected with drain pipe, and sidepiece interface is used to connect nitrogen air induction hose, bottom interface
Discharging tube is connected with, discharging tube is used to connect the waste fluid container for containing waste liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621317575.7U CN206232638U (en) | 2016-12-03 | 2016-12-03 | Improve the Solid-phase synthesis peptides pipe of reactant utilization rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621317575.7U CN206232638U (en) | 2016-12-03 | 2016-12-03 | Improve the Solid-phase synthesis peptides pipe of reactant utilization rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206232638U true CN206232638U (en) | 2017-06-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621317575.7U Expired - Fee Related CN206232638U (en) | 2016-12-03 | 2016-12-03 | Improve the Solid-phase synthesis peptides pipe of reactant utilization rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206232638U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732229A (en) * | 2016-12-03 | 2017-05-31 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction bulb |
-
2016
- 2016-12-03 CN CN201621317575.7U patent/CN206232638U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732229A (en) * | 2016-12-03 | 2017-05-31 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction bulb |
| CN106732229B (en) * | 2016-12-03 | 2019-03-29 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction flask |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170609 Termination date: 20171203 |