CN205067337U - Kidney bean pod mottle virus detecting system of miniflow passageway and long period grating - Google Patents

Abstract

The utility model relates to a kidney bean pod mottle virus detecting system of miniflow passageway and long period grating, the system mainly includes broadband light source, input optic fibre, long period fiber grating, output optical fibre, spectrum appearance, dimethyl silicone polymer miniflow passageway, plastics pipe, syringe pump, waste liquid pool. The utility model discloses the characteristic sensitive to the refracting index according to long period fiber grating, because the refracting index of the kidney bean pod mottle virus solution of different concentration is different, the resonance wave form of the transmitted light attenuation peak of formation is also different on the spectrum appearance. A branch of light of sending the light source is through dimethyl silicone polymer miniflow passageway, measures the kidney bean pod mottle virus solution of different concentration, obtains rather than corresponding resonance wavelength, and the record is observed. Dimethyl silicone polymer miniflow passageway makes the testing result more accurate as the transmission carrier and encapsulation carrier of solution. This system has a great deal of advantages such as simple structure, low cost, direct real -time, safe high efficiency.

Description

The bean pod mottle virus detection system of a kind of microchannel and long-period gratings

Technical field

The utility model relates to bean pod mottle virus detection technique, long period fiber grating sensing, micro-fluidic field, the feature that what utilize is the extraneous refractive index sensitivity characteristic of long-period grating pair, dimethyl silicone polymer can carry out microfluidic control to solution, this analyzer contains the plurality of advantages such as structure is simple, safety sensitive, volume are little, response is quick, corrosion-resistant.

Background technology

Agricultural output problem is the focus that society very pays close attention to always, especially legume, bean pod mottle virus is the fabaceous important viruses such as harm soybean, has very large harmfulness, can cause dropping in production over a large area or even having no harvest of soybean to the growth of soybean.Therefore the detection of bean pod mottle virus is seemed necessary.Being used for still also existing during bean pod mottle virus detects in the traditional inverse transcription polymerase chain reaction technology of use can not many, the complicated operation of quantitative test, experimental procedure, the shortcomings such as experimental period is long, and is difficult to realize on-line real-time measuremen.Therefore the utility model proposes a kind of simply efficient, with low cost, directly real-time bean pod mottle virus detection system.

With fiber optic conduction with collect light signal and the sensor carrying out biological detection is referred to as optical fiber biosensor, it is highly sensitive that it is integrated with that Fibre Optical Sensor itself has, and anti-electromagnetic interference capability is strong, corrosion-resistant, the advantages such as small volume and less weight.

Utility model content

The purpose of this utility model is the problem in order to solve above-mentioned generation, meet the demand of people, propose the bean pod mottle virus detection system of a kind of microchannel and long-period gratings, this system rationally, safety sensitive, volume be little, corrosion-resistant, has a extensive future, result is accurate.

For achieving the above object, the technical solution adopted in the utility model is: the bean pod mottle virus detection system of a kind of microchannel and long-period gratings, is made up of wideband light source, input optical fibre, long period fiber grating, output optical fibre, spectrometer, dimethyl silicone polymer microchannel egative film and substrate, plastic catheter, syringe pump, waste liquid pool.

The utility model utilizes the dimethyl silicone polymer microchannel synthesized by dimethyl silicone polymer substrate and dimethyl silicone polymer egative film long period fiber grating to be encapsulated, and the bean pod mottle virus solution of variable concentrations is injected in long period fiber grating by plastic catheter under the power of syringe pump, solution is fully contacted with long period fiber grating, the light sent from wideband light source is after input optical fibre, the resonance wavelength of transmitted spectrum can change, by observing the transmitted spectrum resonance wave long value on spectrometer, matching obtains the relation of resonance wave long value and bean pod mottle virus solution concentration, the object detecting bean pod mottle virus solution concentration then just can be reached by resonance wave long value.

Wideband light source described in the utility model is as sending incident light, and operating wavelength range is 600nm to 1800nm, and spectral range can comprise the resonance wavelength of inscribed long period fiber grating.

Spectrometer operating wavelength range described in the utility model is 700nm to 1800nm, conveniently connects output optical fibre, and weight is 330g, is easy to carry.

Dimethyl silicone polymer described in the utility model has good chemical stability, electrical insulating property and weatherability, hydrophobicity is good, it is the organic polymer material the most widely based on silicon, nonpoisonous and tasteless, and there is very high anti-shear ability, can at-50 DEG C ~ 200 DEG C Long-Time Service, be applicable to laboratory environment.This microchannel is synthesized by dimethyl silicone polymer substrate and dimethyl silicone polymer egative film, long period fiber grating is placed in this microchannel, the hard factor interference such as ambient temperature change and pressure can be got rid of, give grating and the sufficient contact reaction space of bean pod mottle virus solution.

Plastic catheter diameter described in the utility model is 0.8mm, is divided into input pipe and output duct, and two conduits are inserted in the left and right sides of dimethyl silicone polymer respectively for introducing solution and drawing solution.Sample solution is contained in syringe and enters dimethyl silicone polymer by input pipe under the power of syringe pump, when find solution from output duct out time, think that now solution fully contacts with dimethyl silicone polymer.Each group solution has detected, and injection phosphate buffered saline(PBS) is cleaned repeatedly by input, output duct.

Long period fiber grating described in the utility model is inscribed by single-mode fiber carbon dioxide laser unrelieved stress method for releasing, and improve the sensitivity response of the extraneous refractive index of long-period grating pair by the method for HF corrosion.The effective refractive index of fibre core is determined by the refractive index of fibre core and the refractive index of covering, insensitive to the refractive index of optical fiber surrounding medium.And the effective refractive index of covering is not only responsive to the refractive index of fibre core and covering, also responsive to the refractive index of surrounding medium.When the refractive index of optical fiber surrounding medium changes, cladding-effective-index can be affected, and the result directly caused is the amplitude (T of attenuation peak _m) change and resonance wavelength (λ _m) skew.The change of this spectrum can be used for weighing the refractive index of surrounding medium, can determine the concentration of a predetermined substance.Attenuation peak resonance wavelength can along with the change of surrounding medium refractive index to shortwave or the skew of long wave direction, and the impact of surrounding medium refractive index on resonance wavelength is shown below:

$\frac{{\mathrm{d\λ}}_m}{{\mathrm{dn}}_{sur}}=\frac{{\mathrm{d\λ}}_m}{{\mathrm{dn}}_{eff,m}^{cl}}\[\frac{{\mathrm{dn}}_{eff,m}^{cl}}{{\mathrm{dn}}_{sur}}\]$

Wherein n _surit is the refractive index of surrounding medium.The not cladding mode of same order, formula different.The characteristic of exponent number to long period fiber grating of the cladding mode be therefore coupled has a great impact.Because high-order mode can extend to optical fiber periphery further, therefore high-order cladding mode has better susceptibility to surrounding medium variations in refractive index.When surrounding medium refractive index is less than cladding index (n _sur< n _cl) time, Mode Coupling can be explained with total reflection, and can observe obvious overdamp peak.When surrounding medium refractive index increases, but still when being less than fibre cladding refractive index, attenuation peak is to shortwave direction skew (blue shift), but the intensity of attenuation peak reduces gradually.When the refractive index of surrounding medium levels off to cladding index, wavelength shift is larger, and attenuation peak intensity is less.As surrounding medium refractive index (n identical with cladding index _sur=n _cl) time, covering becomes a medium infinitely extended, and can not produce discrete cladding mode.Now, transmission spectrum does not have obvious attenuation peak, becomes smooth.When surrounding medium refractive index is greater than the refractive index (n of covering _sur> n _cl) time, cladding mode no longer produces total reflection, and carrys out interpretive scheme structure with Fresnel reflection.Because the refractive index of bean pod mottle virus Solutions Solution is all a little more than the refractive index (1.45) of long period fiber grating covering, therefore use the light of Fresnel reflection principle analysis cladding mode in the change at long period fiber grating covering and dielectric interface place, expression formula is:

$R={\left(\frac{n_1-n_2}{n_1+n_2}\right)}^2$

Wherein R is reflectivity, n ₁and n ₂represent the refractive index of long period fiber grating covering and bean pod mottle virus solution respectively.Along with the reduction of bean pod mottle virus solution concentration, refractive index then increases, and reflectivity just diminishes gradually, and the transmitted light intensity of resonance wave strong point also just reduces thereupon, and the degree of depth of corresponding transmitted light attenuation peak will increase gradually.When variable concentrations bean pod mottle virus solution is injected into dimethyl silicone polymer microchannel, n ₂can change, thus the waveform on spectrogram is changed, what obtain from spectrogram is then the movement of wavelength, just can reach the object of mensuration bean pod mottle virus concentration by detecting attenuation peak wavelength value.

The feature advantage that the utility model has is: 1. system architecture is simple, easily operates; 2. the concentration determination of bean pod mottle virus solution is directly real-time; 3. long period fiber grating is placed in dimethyl silicone polymer microchannel and can gets rid of external interference factor, ensure only there is bean pod mottle virus solution concentration to the effect of the resonance wavelength of transmitted spectrum, reduce error largely; 4. long period fiber grating be single-mode fiber carbon dioxide laser unrelieved stress method for releasing inscribe form, and with HF corrosion method improve its to external world refractive index sensitivity response, error can be reduced further; 5. experimental implementation does not relate to hazardous chemicals, and safety is easy to operate, with low cost.

Accompanying drawing explanation

Fig. 1 is structural representation of the present utility model

Fig. 2 is experimental result picture of the present utility model

Embodiment

The temperature and humidity conditions that the utility model is suitable for is: > 15 DEG C, 0-90%RH.

As shown in Figure 1, it is the bean pod mottle virus detection system of a kind of microchannel and long-period gratings, the spectral range of wideband light source (1) is 600nm-1800nm, the incident light sent enters the dimethyl silicone polymer microchannel synthesized by dimethyl silicone polymer substrate (5) and dimethyl silicone polymer egative film (8) by input optical fibre (2), long period fiber grating (7) is wherein housed, its resonance wavelength is 1535.6nm, under the power of syringe pump (3), dimethyl silicone polymer microchannel is entered by input pipe (4) with the syringe that tested bean pod mottle virus solution is housed, when there being solution out to enter into waste liquid pool (11) from output duct (6), stop syringe pump work, leave standstill after 3 minutes, obtain the harmonic wave being arrived the upper transmitted light attenuation peak formed of spectrometer (10) by output optical fibre (9), and it is fixing on spectrometer, then repeatedly clean by phosphate buffered saline(PBS), harmonic wave figure is made to return to oscillogram in air, measure the bean pod mottle virus solution of variable concentrations more successively, obtain resonance wave long value corresponding thereto, record and observe.

As shown in Figure 2, this is the experimental result picture obtained in laboratory.Fig. 2 represents the transmitted spectrum of the bean pod mottle virus solution of variable concentrations, and along with the change of concentration, the harmonic wave being arrived the transmitted light attenuation peak that spectrometer is formed by output optical fibre is different, can detect bean pod mottle virus solution thus.

Know with those skilled in the art know that; according to method of the present utility model; can to other biological sensory field as some Viral diagnosis concrete etc.; wideband light source, long period fiber grating, dimethyl silicone polymer microchannel, light power meter can carry out the unified collocation of new wavelength; apparatus structure can be optimized design, and protection domain of the present utility model is not limited to above embodiment.

Claims (4)

1. the bean pod mottle virus detection system of a microchannel and long-period gratings, it is characterized in that by wideband light source (1), input optical fibre (2), syringe pump (3), input pipe (4), dimethyl silicone polymer substrate (5), output duct (6), long period fiber grating (7), dimethyl silicone polymer egative film (8), output optical fibre (9), spectrometer (10), waste liquid pool (11) forms, the incident light that described wideband light source (1) sends enters the dimethyl silicone polymer microchannel synthesized by dimethyl silicone polymer substrate (5) and dimethyl silicone polymer egative film (8) by input optical fibre (2), long period fiber grating (7) is wherein housed, and under the power of syringe pump (3), enter dimethyl silicone polymer microchannel by input pipe (4) with the syringe that tested bean pod mottle virus solution is housed, when there being solution out to enter into waste liquid pool (11) from output duct (6), stop syringe pump work, leave standstill after 3 minutes, obtain the harmonic wave being arrived the upper transmitted light attenuation peak formed of spectrometer (10) by output optical fibre (9), and it is fixing on spectrometer, obtain the resonance wave long value corresponding with bean pod mottle virus content, realize the detection of bean pod mottle virus.

2. the bean pod mottle virus detection system of a kind of microchannel according to claim 1 and long-period gratings, it is characterized in that: described wideband light source (1) operating wavelength range is 600nm to 1800nm, spectral range can comprise the resonance wavelength of inscribed long period fiber grating (7).

3. the bean pod mottle virus detection system of a kind of microchannel according to claim 1 and long-period gratings, it is characterized in that: described spectrometer (10) operating wavelength range is 700nm ~ 1800nm, can connect output optical fibre, weight is 330g.

4. the bean pod mottle virus detection system of a kind of microchannel according to claim 1 and long-period gratings, is characterized in that: described dimethyl silicone polymer substrate (5) and dimethyl silicone polymer egative film (8) are the organic polymer materials based on silicon.