CN205046126U - Acute biotoxicity detection device of water - Google Patents
Acute biotoxicity detection device of water Download PDFInfo
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- CN205046126U CN205046126U CN201520606496.7U CN201520606496U CN205046126U CN 205046126 U CN205046126 U CN 205046126U CN 201520606496 U CN201520606496 U CN 201520606496U CN 205046126 U CN205046126 U CN 205046126U
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- water sample
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- oxygen sensor
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Abstract
The utility model relates to an acute biotoxicity detection device of water, its characteristics are, include: constant temperature sealed container by the splendid attire water sample, the gas -supply pipe, the air closed circulation system that circulation air pump and oxygen sensor constitute, display controller, place the quilt survey water sample that possesies certain volume and contain good oxygen nature microorganism in the constant temperature sealed container of splendid attire water sample, is containing being surveyed in the top volume of water sample to the air of good oxygen nature microorganism, the air intake duct end of gas -supply pipe is stretched in the water sample is surveyed to the quilt that contains good oxygen nature microorganism, the air of gas -supply pipe is given vent to anger the pipe end and is stretched in the air in the constant temperature sealed container of splendid attire water sample, circulation air pump dress is on the gas -supply pipe, it concatenates oxygen sensor to give vent to anger the pipe end at the air of gas -supply pipe, the circulation air pump, oxygen sensor and display controller all are connected with the display controller electricity. Has simple structure, advantages such as detect operation is simple and convenient, quick, accuracy.
Description
Technical field
The utility model relates to biochemical field, is water body acute biological toxicity proofing unit.
Background technology
So-called acute biological toxicity, refers to that normal water body is subject to suddenly the pollution of toxic substance, and causes harm to biological group.Along with the quickening of process of industrialization and the development of chemical industry, the pollution to environment water of various chemical substance, heavy metal substance is more and more serious.Particularly these pollutions also exist sudden and Unpredictability, work the mischief to water ecology and HUMAN HEALTH.If there is can not Timeliness coverage at urban drinking water waterhead area, more can lead to milestone and even bring out social concern.Therefore in the urgent need to carrying out continuously real-time online acute toxicity qualification to relevant water body.The harm of toxicity to biological group is only paid close attention in this qualification, and can not identify kind and the source of toxicity.
Based on the demand, occurred the bio-toxicity proofing unit based on fish, water flea class, luminescent bacteria in recent years, there is complex structure, maintenance cost is high, is difficult to the needs meeting waterhead area automatic continuous monitoring in the wild.
Summary of the invention
Design principle of the present utility model is that in water, aerobic microorganism wants growth and breeding under normal condition of water quality, consumes organism and oxygen simultaneously.After toxicant enters water body suddenly, microbic activity is suppressed immediately, and its oxygen-consumption declines and even stops oxygen consumption.Judge its activity change by the oxygen-consumption change of monitoring these microorganisms, indirectly reflect acute change and the toxicity intensity of water body toxicity.
The purpose of this utility model is, overcomes the deficiencies in the prior art, provides a kind of structure simple, detects easy and simple to handle, water body acute biological toxicity proofing unit fast and accurately.
The technical scheme realizing the employing of one of the utility model object is, a kind of water body acute biological toxicity proofing unit, it is characterized in that, it comprises: by the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, the air containment recycle system that circulation air pump and oxygen sensor are formed, display control unit, the detected water sample of occupying a constant volume and containing aerobic microorganism is placed in the constant-temperature enclosed container of splendid attire water sample, be air in volume above the detected water sample containing aerobic microorganism, the air inlet pipe end of pneumatic tube is stretched in the constant-temperature enclosed container of splendid attire water sample containing in the detected water sample of aerobic microorganism, the air of pneumatic tube is given vent to anger in air that pipe end stretches in the constant-temperature enclosed container of splendid attire water sample, circulation air pump is contained on pneumatic tube, the air of pneumatic tube give vent to anger pipe end serial connection oxygen sensor, circulation air pump, oxygen sensor and display control unit are all electrically connected with display control unit.
Water body acute biological toxicity proofing unit of the present utility model, compared with existing device, the utility model has the advantage of:
1, with microorganism, particular requirement is not had, such as, without the need to the hydrobiont of screening and culturing particular variety, luminescent bacteria etc. to test.In common water, the aerobic microorganism of aerobic microorganism or cultivation naturally can meet detection requirement;
2, structure is simple, and without the need to optical pickocff and the signal handling equipment of complexity, oxygen sensor structure is simple, is easy to working service, cheap;
3, without the need to adding medicaments, environment is not polluted;
4, be easy to realize industrial design, formation is entered automatically, stock layout water, automatic continuous monitoring, the on-line checkingi instrument that automatic analysis compares and reports to the police, there is economic worth and social value widely, be applicable to river, river, lake, storehouse, underground water, seawater and other water body acute biological toxicities detect, of many uses.
5, detect easy and simple to handle, quick, accurate.
Accompanying drawing explanation
Fig. 1 is water body acute biological toxicity structure of the detecting device schematic diagram of the present utility model;
Fig. 2 is oxygen concn in actual test process-time curve schematic diagram;
Fig. 3 is yeast aerobic repiration total time t under different toluene level condition
alwayschange curve schematic diagram.
In figure: 1 containing the detected water sample of aerobic microorganism, the constant-temperature enclosed container of 2 splendid attire water samples, 3 pneumatic tubes, 4 circulation air pumps, 5 oxygen sensors, 6 display control units.
Embodiment
By diagram, the utility model is described in further detail below.
With reference to Fig. 1, a kind of water body acute biological toxicity proofing unit of the present utility model, comprise: by the constant-temperature enclosed container 2 of splendid attire water sample, pneumatic tube 3, the air containment recycle system that circulation air pump 4 and oxygen sensor 5 are formed, display control unit 6, the detected water sample 1 of occupying a constant volume and containing aerobic microorganism is placed in the constant-temperature enclosed container 2 of splendid attire water sample, be air in volume above the detected water sample 1 containing aerobic microorganism, the air inlet pipe end of pneumatic tube 3 is stretched in the constant-temperature enclosed container 2 of splendid attire water sample containing in the detected water sample 1 of aerobic microorganism, the air of pneumatic tube 3 is given vent to anger in air that pipe end stretches in the constant-temperature enclosed container 2 of splendid attire water sample, circulation air pump 4 is contained on pneumatic tube 3, the air of pneumatic tube 3 give vent to anger pipe end serial connection oxygen sensor 5, circulation air pump 4 and oxygen sensor 5 are all electrically connected with display control unit 6.Circulation air pump 4, oxygen sensor 5 and display control unit 6 is commercially available prod.
When using water body acute biological toxicity proofing unit of the present utility model to carry out water body detection:
1) by the air containment recycle system that the constant-temperature enclosed container 2 of splendid attire water sample, pneumatic tube 3, circulation air pump 4 and oxygen sensor 5 connect and compose;
2) the constant-temperature enclosed container 2 of splendid attire water sample is interior places the detected water sample 1 of occupying a constant volume and containing aerobic microorganism, is air above the detected water sample 1 containing aerobic microorganism in volume;
3) start circulation air pump 4, the air ordering about constant-temperature enclosed container 2 internal upper part of splendid attire water sample circulates along the air containment recycle system;
4) when airflow is through oxygen sensor 5, oxygen concn value converts electric signal transmission to display control unit 6 by oxygen sensor 5, and display control unit 6 records oxygen concn curve over time;
5), under or low toxicity condition nontoxic at water sample, the breeding of microorganism normal growth constantly consumes organism in the constant-temperature enclosed container 2 of splendid attire water sample and oxygen, causes oxygen concn fall off rate to be accelerated; Under water sample toxic conditions, microorganism active is suppressed even dormancy or death, and oxygen concn fall off rate is slack-off or do not decline, and is compared by nontoxic water sample, just can realize the Monitoring Acute Toxicity of water body with unknown toxicity water sample oxygen concn-time curve.
Experimental formula
Accurately take commercially available Angel Yeast powder 0.2g, 5% glucose 10ml, 50g/LKH
2pO
450 ~ 55ml and q. s. toluene, join above-mentioned materials in test bottle, and ensure that in bottle, overall solution volume is 65ml, total volume of air is 15ml.Open instrument when water bath heating temperature is 31 DEG C to test.
Experimental result
Fig. 2 is in actual test process, oxygen concn-time changing curve in closed system.Find through great many of experiments, curve is made up of two portions, is the slow district of AB section and the logarithmic region of BC section respectively.When AB section, yeast is in slow state of activation, and respiration is faint, and oxygen-consumption is less, and curve presents intimate straight line tendency.After the time reaches B point, yeast is in efficient active state, and is exceedingly fast with stable geometricprogression and increases, and oxygen-consumption increases greatly, and within a short period of time, system oxygen level reduced rapidly.After the time reaches C point, in system, oxygen level is zero, and yeast carries out anaerobic respiration.
Yeast aerobic repiration each elementary reaction time under table 1, different toluene level condition gathers
Discussion of results: as can be seen from Table 1, along with the increase of toluene consumption, yeast aerobic repiration process is suppressed, and breathing time increases thereupon.When toluene level is increased to 63.4mg/L, saccharomycetic breathing starts to be suppressed, t
1time has increased slightly, t
2time variations amplitude is little.When continuing to increase toluene level to 84.6mg/L, restraining effect becomes obvious, t
1time period adds about 5min, t
2time period increases about 3min.When the content of toluene reaches 108mg/L, the toxicity of toluene creates very large impact to saccharomycetic aerobic repiration, shows t
1with t
2all there is very large increasing degree in the time period, causes and blank assay Comparative result, aerobic repiration add about 18min total time.On the whole, by comparing t
1the section time can find out the impact of the increase of toluene concentration on yeast aerobic repiration, if in conjunction with t
1and t
2the two, then more can compare toluene intuitively and accurately to saccharomycetic acute biological value.Domestic relevant research once adopted Xiphophorus helleri to carry out acute biological toxicity test as study subject, and result display reaches its acute toxicity value when toluene concentration reaches 104mg/L.Composition graphs 3, can know in contrast and find out that the utility model reaction is more sensitive, toxic concentration Monitoring lower-cut is low to moderate 63.4mg/L and having good stability of system, has very large potentiality and using value in following acute biological toxicity context of detection.Above content is in conjunction with concrete preferred implementation further detailed description of the utility model; can not assert that concrete enforcement of the present utility model is confined to these explanations; for the utility model person of an ordinary skill in the technical field; without departing from the concept of the premise utility; some simple deduction or replace can also be made, all should be considered as belonging to protection domain of the present utility model.
Claims (1)
1. a water body acute biological toxicity proofing unit, it is characterized in that, it comprises: by the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, the air containment recycle system that circulation air pump and oxygen sensor are formed, display control unit, the air inlet pipe end of described pneumatic tube is stretched in the lower volume of the constant-temperature enclosed container of splendid attire water sample, the air of described pneumatic tube pipe end of giving vent to anger is stretched in the oral area volume of the constant-temperature enclosed container of splendid attire water sample, circulation air pump is contained on pneumatic tube, the air of pneumatic tube give vent to anger pipe end serial connection oxygen sensor, circulation air pump, oxygen sensor and display control unit are all electrically connected with display control unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520606496.7U CN205046126U (en) | 2015-08-13 | 2015-08-13 | Acute biotoxicity detection device of water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520606496.7U CN205046126U (en) | 2015-08-13 | 2015-08-13 | Acute biotoxicity detection device of water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205046126U true CN205046126U (en) | 2016-02-24 |
Family
ID=55339724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520606496.7U Active CN205046126U (en) | 2015-08-13 | 2015-08-13 | Acute biotoxicity detection device of water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205046126U (en) |
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2015
- 2015-08-13 CN CN201520606496.7U patent/CN205046126U/en active Active
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