CN105062880A - Device and method for detecting acute biotoxicity of water body - Google Patents
Device and method for detecting acute biotoxicity of water body Download PDFInfo
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- CN105062880A CN105062880A CN201510493786.XA CN201510493786A CN105062880A CN 105062880 A CN105062880 A CN 105062880A CN 201510493786 A CN201510493786 A CN 201510493786A CN 105062880 A CN105062880 A CN 105062880A
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- water sample
- air
- constant
- splendid attire
- oxygen sensor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
Abstract
The invention provides a device for detecting the acute biotoxicity of a water body. The device is characterized by comprising an air closed circulation system and a display controller, wherein the air closed circulation system comprises a constant-temperature closed vessel filled with a water sample, an air delivery pipe, a circulating air pump and an oxygen sensor; the tested water sample occupying certain volume and containing aerobic microbes is arranged in the constant-temperature closed vessel filled with the water sample, air is accommodated in space above the tested water sample containing the aerobic microbes, an air inlet end of the air delivery pipe extends into the tested water sample containing the aerobic microbes, an air outlet end of the air delivery pipe extends in the air in the constant-temperature closed vessel filled with the water sample, the circulating air pump is mounted on the air delivery pipe, the oxygen sensor is connected in series to the air outlet end of the air delivery pipe, and the circulating air pump and the oxygen sensor are electrically connected with the display controller. The device has the advantages of simple structure, simplicity, convenience, rapidness and accuracy in detection operation and the like. A detection method which is scientific, reasonable, free of environmental pollution and wide in application range is further provided.
Description
Technical field
The present invention relates to biochemical field, is a kind of water body acute biological toxicity proofing unit and method thereof.
Technical background
So-called acute biological toxicity, refers to that normal water body is subject to suddenly the pollution of toxic substance, and causes harm to biological group.Along with the quickening of process of industrialization and the development of chemical industry, the pollution to environment water of various chemical substance, heavy metal substance is more and more serious.Particularly these pollutions also exist sudden and Unpredictability, work the mischief to water ecology and HUMAN HEALTH.If there is can not Timeliness coverage at urban drinking water waterhead area, more can lead to milestone and even bring out social concern.Therefore in the urgent need to carrying out continuously real-time online acute toxicity qualification to relevant water body.The harm of toxicity to biological group is only paid close attention in this qualification, and can not identify kind and the source of toxicity.
Based on the demand, there is the bio-toxicity detection method based on fish, water flea class, luminescent bacteria in recent years, first two is that luminescent bacteria is the power based on sending fluorescence, and bacterium source needs special screening and culturing based on directly observing biological physical activity state in water.The apparatus structure applying these methods is complicated, and maintenance cost is high, is difficult to realize waterhead area automatic continuous monitoring in the wild.
Summary of the invention
Design principle of the present invention is that in water, aerobic microorganism wants growth and breeding under normal condition of water quality, consumes organism and oxygen simultaneously.After toxicant enters water body suddenly, microbic activity is suppressed immediately, and its oxygen-consumption declines and even stops oxygen consumption.Judge its activity change by the oxygen-consumption change of monitoring these microorganisms, indirectly reflect acute change and the toxicity intensity of water body toxicity.
The object of the invention is, overcome the deficiencies in the prior art, provide a kind of structure simple, detect easy and simple to handle, water body acute biological toxicity proofing unit fast and accurately; And provide scientific and reasonable, environmentally safe, water body acute biological toxicity detection method applied widely.
The technical scheme realizing the employing of one of the object of the invention is, a kind of water body acute biological toxicity proofing unit, it is characterized in that, it comprises: by the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, the air containment recycle system that circulation air pump and oxygen sensor are formed, display control unit, the detected water sample of occupying a constant volume and containing aerobic microorganism is placed in the constant-temperature enclosed container of splendid attire water sample, be air in volume above the detected water sample containing aerobic microorganism, the air inlet pipe end of pneumatic tube is stretched in the constant-temperature enclosed container of splendid attire water sample containing in the detected water sample of aerobic microorganism, the air of pneumatic tube is given vent to anger in air that pipe end stretches in the constant-temperature enclosed container of splendid attire water sample, circulation air pump is contained on pneumatic tube, the air of pneumatic tube give vent to anger pipe end serial connection oxygen sensor, circulation air pump, oxygen sensor and display control unit are all electrically connected with display control unit.
Two technical schemes adopted realizing the object of the invention are, a kind of water body acute biological toxicity detection method, and it is characterized in that, it has in comprising:
1) water body acute biological toxicity proofing unit is used, by the air containment recycle system that the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, circulation air pump and oxygen sensor connect and compose;
2) the constant-temperature enclosed container of splendid attire water sample is interior places the detected water sample of occupying a constant volume and containing aerobic microorganism, is air above the detected water sample containing aerobic microorganism in volume;
3) start circulation air pump, the air ordering about the constant-temperature enclosed container internal upper part of splendid attire water sample circulates along the air containment recycle system;
4) when airflow is through oxygen sensor, oxygen concn value converts electric signal transmission to display control unit by oxygen sensor, display control unit record oxygen concn curve over time;
5), under or low toxicity condition nontoxic at water sample, the breeding of microorganism normal growth constantly consumes organism in the constant-temperature enclosed container of splendid attire water sample and oxygen, causes oxygen concn fall off rate to be accelerated; Under water sample toxic conditions, microorganism active is suppressed even dormancy or death, and oxygen concn fall off rate is slack-off or do not decline, and is compared by nontoxic water sample, just can realize the Monitoring Acute Toxicity of water body with unknown toxicity water sample oxygen concn-time curve.
Water body acute biological toxicity proofing unit of the present invention, compared with existing device, the invention has the advantages that:
1, with microorganism, particular requirement is not had, such as, without the need to the hydrobiont of screening and culturing particular variety, luminescent bacteria etc. to test.In common water, the aerobic microorganism of aerobic microorganism or cultivation naturally can meet detection requirement;
2, structure is simple, and without the need to optical pickocff and the signal handling equipment of complexity, oxygen sensor structure is simple, is easy to working service, cheap;
3, without the need to adding medicaments, environment is not polluted;
4, be easy to realize industrial design, formed automatically enter, stock layout water, automatic continuous monitoring, the on-line checkingi instrument that automatic analysis compares and reports to the police, has economic worth and social value widely.
5, detect easy and simple to handle, quick, accurate.
Water body acute biological toxicity detection method of the present invention is scientific and reasonable, environmentally safe, is applicable to river, river, lake, storehouse, underground water, seawater and other water body acute biological toxicities and detects, of many uses.
Accompanying drawing explanation
Fig. 1 is water body acute biological toxicity structure of the detecting device schematic diagram of the present invention;
Fig. 2 is oxygen concn in actual test process-time curve schematic diagram;
Fig. 3 is yeast aerobic repiration total time t under different toluene level condition
alwayschange curve schematic diagram.
In figure: 1 containing the detected water sample of aerobic microorganism, the constant-temperature enclosed container of 2 splendid attire water samples, 3 pneumatic tubes, 4 circulation air pumps, 5 oxygen sensors, 6 display control units.
Embodiment
By diagram, the invention will be further described below.
With reference to Fig. 1, a kind of water body acute biological toxicity proofing unit of the present invention, comprise: by the constant-temperature enclosed container 2 of splendid attire water sample, pneumatic tube 3, the air containment recycle system that circulation air pump 4 and oxygen sensor 5 are formed, display control unit 6, the detected water sample 1 of occupying a constant volume and containing aerobic microorganism is placed in the constant-temperature enclosed container 2 of splendid attire water sample, be air in volume above the detected water sample 1 containing aerobic microorganism, the air inlet pipe end of pneumatic tube 3 is stretched in the constant-temperature enclosed container 2 of splendid attire water sample containing in the detected water sample 1 of aerobic microorganism, the air of pneumatic tube 3 is given vent to anger in air that pipe end stretches in the constant-temperature enclosed container 2 of splendid attire water sample, circulation air pump 4 is contained on pneumatic tube 3, the air of pneumatic tube 3 give vent to anger pipe end serial connection oxygen sensor 5, circulation air pump 4 and oxygen sensor 5 are all electrically connected with display control unit 6.Circulation air pump 4, oxygen sensor 5 and display control unit 6 is commercially available prod.
A kind of water body acute biological toxicity detection method of the present invention, has in comprising:
1) water body acute biological toxicity proofing unit is used, by the air containment recycle system that the constant-temperature enclosed container 2 of splendid attire water sample, pneumatic tube 3, circulation air pump 4 and oxygen sensor 5 connect and compose;
2) the constant-temperature enclosed container 2 of splendid attire water sample is interior places the detected water sample 1 of occupying a constant volume and containing aerobic microorganism, is air above the detected water sample 1 containing aerobic microorganism in volume;
3) start circulation air pump 4, the air ordering about constant-temperature enclosed container 2 internal upper part of splendid attire water sample circulates along the air containment recycle system;
4) when airflow is through oxygen sensor 5, oxygen concn value converts electric signal transmission to display control unit 6 by oxygen sensor 5, and display control unit 6 records oxygen concn curve over time;
5), under or low toxicity condition nontoxic at water sample, the breeding of microorganism normal growth constantly consumes organism in the constant-temperature enclosed container 2 of splendid attire water sample and oxygen, causes oxygen concn fall off rate to be accelerated; Under water sample toxic conditions, microorganism active is suppressed even dormancy or death, and oxygen concn fall off rate is slack-off or do not decline, and is compared by nontoxic water sample, just can realize the Monitoring Acute Toxicity of water body with unknown toxicity water sample oxygen concn-time curve.
Experimental formula
Accurately take commercially available Angel Yeast powder 0.2g, 5% glucose 10ml, 50g/LKH
2pO
450 ~ 55ml and q. s. toluene, join above-mentioned materials in test bottle, and ensure that in bottle, overall solution volume is 65ml, total volume of air is 15ml.Open instrument when water bath heating temperature is 31 DEG C to test.
Experimental result
Fig. 2 is in actual test process, oxygen concn-time changing curve in closed system.Find through great many of experiments, curve is made up of two portions, is the slow district of AB section and the logarithmic region of BC section respectively.When AB section, yeast is in slow state of activation, and respiration is faint, and oxygen-consumption is less, and curve presents intimate straight line tendency.After the time reaches B point, yeast is in efficient active state, and is exceedingly fast with stable geometricprogression and increases, and oxygen-consumption increases greatly, and within a short period of time, system oxygen level reduced rapidly.After the time reaches C point, in system, oxygen level is zero, and yeast carries out anaerobic respiration.
Yeast aerobic repiration each elementary reaction time under table 1, different toluene level condition gathers
Discussion of results: as can be seen from Table 1, along with the increase of toluene consumption, yeast aerobic repiration process is suppressed, and breathing time increases thereupon.When toluene level is increased to 63.4mg/L, saccharomycetic breathing starts to be suppressed, t
1time has increased slightly, t
2time variations amplitude is little.When continuing to increase toluene level to 84.6mg/L, restraining effect becomes obvious, t
1time period adds about 5min, t
2time period increases about 3min.When the content of toluene reaches 108mg/L, the toxicity of toluene creates very large impact to saccharomycetic aerobic repiration, shows t
1with t
2all there is very large increasing degree in the time period, causes and blank assay Comparative result, aerobic repiration add about 18min total time.On the whole, by comparing t
1the section time can find out the impact of the increase of toluene concentration on yeast aerobic repiration, if in conjunction with t
1and t
2the two, then more can compare toluene intuitively and accurately to saccharomycetic acute biological value.Domestic relevant research once adopted Xiphophorus helleri to carry out acute biological toxicity test as study subject, and result display reaches its acute toxicity value when toluene concentration reaches 104mg/L.Composition graphs 3, can know in contrast and find out that the method applied in the present invention reaction is more sensitive, toxic concentration Monitoring lower-cut is low to moderate 63.4mg/L and having good stability of system, has very large potentiality and using value in following acute biological toxicity context of detection.Above content is in conjunction with concrete preferred implementation further description made for the present invention; can not assert that specific embodiment of the invention is confined to these explanations; for general technical staff of the technical field of the invention; without departing from the inventive concept of the premise; some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (2)
1. a water body acute biological toxicity proofing unit, it is characterized in that, it comprises: by the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, the air containment recycle system that circulation air pump and oxygen sensor are formed, display control unit, the detected water sample of occupying a constant volume and containing aerobic microorganism is placed in the constant-temperature enclosed container of splendid attire water sample, be air in volume above the detected water sample containing aerobic microorganism, the air inlet pipe end of pneumatic tube is stretched in the constant-temperature enclosed container of splendid attire water sample containing in the detected water sample of aerobic microorganism, the air of pneumatic tube is given vent to anger in air that pipe end stretches in the constant-temperature enclosed container of splendid attire water sample, circulation air pump is contained on pneumatic tube, the air of pneumatic tube give vent to anger pipe end serial connection oxygen sensor, circulation air pump, oxygen sensor and display control unit are all electrically connected with display control unit.
2. a water body acute biological toxicity detection method, is characterized in that, it has in comprising:
1) water body acute biological toxicity proofing unit is used, by the air containment recycle system that the constant-temperature enclosed container of splendid attire water sample, pneumatic tube, circulation air pump and oxygen sensor connect and compose;
2) the constant-temperature enclosed container of splendid attire water sample is interior places the detected water sample of occupying a constant volume and containing aerobic microorganism, is air above the detected water sample containing aerobic microorganism in volume;
3) start circulation air pump, the air ordering about the constant-temperature enclosed container internal upper part of splendid attire water sample circulates along the air containment recycle system;
4) when airflow is through oxygen sensor, oxygen concn value converts electric signal transmission to display control unit by oxygen sensor, display control unit record oxygen concn curve over time;
5), under or low toxicity condition nontoxic at water sample, the breeding of microorganism normal growth constantly consumes organism in the constant-temperature enclosed container of splendid attire water sample and oxygen, causes oxygen concn fall off rate to be accelerated; Under water sample toxic conditions, microorganism active is suppressed even dormancy or death, and oxygen concn fall off rate is slack-off or do not decline, and is compared by nontoxic water sample, just can realize the Monitoring Acute Toxicity of water body with unknown toxicity water sample oxygen concn-time curve.
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| CN201510493786.XA CN105062880A (en) | 2015-08-13 | 2015-08-13 | Device and method for detecting acute biotoxicity of water body |
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| CN201510493786.XA CN105062880A (en) | 2015-08-13 | 2015-08-13 | Device and method for detecting acute biotoxicity of water body |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231438A (en) * | 2018-11-07 | 2019-01-18 | 山东建筑大学 | A kind of saprobia toxicity on-line monitoring early warning system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102735812A (en) * | 2012-06-15 | 2012-10-17 | 中国科学院长春应用化学研究所 | Biochemical oxygen demand detection method |
| CN203249892U (en) * | 2013-04-17 | 2013-10-23 | 陈威 | Biochemical oxygen demand measuring device |
| CN104820082A (en) * | 2015-05-21 | 2015-08-05 | 中国神华能源股份有限公司 | Wastewater toxicity detection system and wastewater toxicity detection method |
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2015
- 2015-08-13 CN CN201510493786.XA patent/CN105062880A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102735812A (en) * | 2012-06-15 | 2012-10-17 | 中国科学院长春应用化学研究所 | Biochemical oxygen demand detection method |
| CN203249892U (en) * | 2013-04-17 | 2013-10-23 | 陈威 | Biochemical oxygen demand measuring device |
| CN104820082A (en) * | 2015-05-21 | 2015-08-05 | 中国神华能源股份有限公司 | Wastewater toxicity detection system and wastewater toxicity detection method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231438A (en) * | 2018-11-07 | 2019-01-18 | 山东建筑大学 | A kind of saprobia toxicity on-line monitoring early warning system |
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Application publication date: 20151118 |