CN2049359U - 活性维生素d检测药盒 - Google Patents

活性维生素d检测药盒 Download PDF

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CN2049359U
CN2049359U CN 89208520 CN89208520U CN2049359U CN 2049359 U CN2049359 U CN 2049359U CN 89208520 CN89208520 CN 89208520 CN 89208520 U CN89208520 U CN 89208520U CN 2049359 U CN2049359 U CN 2049359U
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vitamin
inspection box
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石育原
张培茵
李亚娟
谷秀彬
于贵
仪明光
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BETHUNE MEDICAL UNIVERSITY
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BETHUNE MEDICAL UNIVERSITY
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Abstract

活性维生素D检测药盒。
本实用新型属生物化学领域。
该检测药盒是由盒底、盒盖组成的。盒内有受体蛋白，标准品，标记物，磷酸缓冲液，活性炭等五种试剂所组成。
本检测药盒使用方法简便，灵敏、快速、所用血清样品少，容易为患者所接受。

Description

活性维生素D检测药盒。
本发明属生物化学领域。
活性维生素D也称为1.25－双羟胆钙化醇。（1.25－Di－hydrOXychO    LecaLciferOL.DHec）。是维生素D在体内经肝脏和肾脏羟化后转变而成的一种高活性形式。在正常情况下，体内合成量充足，合成后经血液运输到靶器官，通过受体而发挥作用，所以它是一种新的甾族激素。活性维生素D参与免疫调节，促进某些肿瘤细胞（如白血病细胞）的正常分化以及对生长素，泌乳素分泌等都有重要影响，对佝偻病的早期诊断和防治，对老年性骨病和某些内分泌疾病的研究有应用价值。同时，血清中活性维生素D含量的检测对奶牛产奶率，家禽产蛋率，淡水养鱼及实验动物的饲养条件，饲料配方等均有重要的指导意义。
活性维生素D检测测方法国外已开展，但方法的技术难度较大，需要昂贵的仪器设备，不经专门的训练难以掌握操作技术。受体蛋白制作技术复杂，周期长，不稳定，测量血清量大，不易为患者所接受。目前国内尚没有理想的测试手段和方法，更没有试剂盒的供应和使用。
本发明的目的是为了在国内开展活性维生素D检测技术，制备国产化试剂盒，使此项超微量检测技术尽快地为经济建设服务。
本实用新型的结构示意图由附图给出。图1为盒盖，图2为盒底。盒内容物装入盒底（1）中，内容物包括：受体蛋白（2），标准品（3），标记物（4），磷酸缓冲液（5），活性炭（6），各种试剂的制备如下：
1.1.受体蛋白（2）的制备：以正常兔小肠胞浆受体蛋白制备为例。用日本大白兔活体积取小肠环，灌洗肠腔，纵向剖开，刮下粘膜层，用2倍体积的高渗缓冲液制备匀浆，离心，取上清液超速离心，上清液测蛋白浓度后，分装，通氮条件下抽干，冷冻于玻璃瓶中。
2.标准品（3）的制备：以结晶维生素D3为原料，采用体外羟化的方法进行孵育，经葡聚糖凝胶LH－20柱层析及高压液相分离，制得1.25－（OH）－D，分装于玻璃瓶中。
3.标记物（4）的制备：活性维生素D的氚标记物采用低温交换法。以催化剂为钯，氚离子由氚分子激发获得。粗标记样品经真空脱游离氚后，经葡聚糖凝胶LH－20柱层析分离，纯化，分装于玻璃瓶中。－20℃水箱保存。比活性：87ci／mmOL。
4.磷酸缓冲液（5）的配制：用磷酸二氢钠和磷酸氢二钠加氮化钠溶于重蒸水中，溶均即可，分装于玻璃瓶中。
5.活性炭（6）的制备：取活性炭经酸，碱及重蒸水清洗后烘烤，分装瓶中，密封保存。
上述试剂装入盒底，用泡沫塑料固定，封上盖即可。
使用时取静脉血离心，取血清1mL，按侧定程序及计算公式便可可测定血清中活性维生素D含量。本试剂盒测定正常值为20－65pg／mL。
本发明药盒使用方法简便，灵敏，快速，不需要高效液相等昂贵贵的仪器，一般市级医院均可开展此项技术。检测方法稳定，精密度及回收率均合格，所用血清样品量少，容易被患者所接受。

Claims (1)

1、一种由盒底(1)和盒盖组成的活性维生素D检测药盒，其特征在于盒底内受体蛋白(2)，标准品(3)，标记物(4)，磷酸缓冲液(5)，活性炭(6)所组成。
CN 89208520 1989-06-11 1989-06-11 活性维生素d检测药盒 Withdrawn CN2049359U (zh)

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CN 89208520 CN2049359U (zh) 1989-06-11 1989-06-11 活性维生素d检测药盒

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104969067A (zh) * 2013-02-06 2015-10-07 富士瑞必欧株式会社 维生素d的测定方法及测定用试剂盒
CN105556313A (zh) * 2013-09-17 2016-05-04 生物梅里埃公司 用于从维生素d结合蛋白解离维生素d的溶液、相关的检测方法及用途

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104969067A (zh) * 2013-02-06 2015-10-07 富士瑞必欧株式会社 维生素d的测定方法及测定用试剂盒
US10571479B2 (en) 2013-02-06 2020-02-25 Fujirebio Inc. Vitamin D measurement method and measurement kit
CN105556313A (zh) * 2013-09-17 2016-05-04 生物梅里埃公司 用于从维生素d结合蛋白解离维生素d的溶液、相关的检测方法及用途
CN105556313B (zh) * 2013-09-17 2018-08-24 生物梅里埃公司 用于从维生素d结合蛋白解离维生素d的溶液、相关的检测方法及用途

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