CN204925130U - Remaining immunofluorescence kit of avermectin in detection beef - Google Patents
Remaining immunofluorescence kit of avermectin in detection beef Download PDFInfo
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- CN204925130U CN204925130U CN201520690822.7U CN201520690822U CN204925130U CN 204925130 U CN204925130 U CN 204925130U CN 201520690822 U CN201520690822 U CN 201520690822U CN 204925130 U CN204925130 U CN 204925130U
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Abstract
The utility model discloses a remaining immunofluorescence kit of avermectin in detection beef, including box -shaped shell, immunochromatographic test strip, this immunochromatographic test strip establishes in the box -shaped shell, immunochromatographic test strip includes sample pad, analysis membrane, the pad that absorbs water, viscidity end liner and combine to fill up, and the analysis is epimembranal, and be equipped with the detection area of the anti avermectin monoclonal antibody of peridium and peridium goat -anti mouse lgG's matter accuse is taken, box -shaped shell top is equipped with sample interpolation hole, the result reads window and terminal point instruction window, and the sample adds the hole and corresponds with sample pad location, and the result reads the position that the window was taken and the matter accuse is taken with the detection and corresponds, and the terminal point instructs the window to correspond with the pad location that absorbs water. This kit adopts rotates upward luminous technique and combines with the immunological technique, suits at the short -term test at scene, with low costs, is suitable for the examination to a large amount of samples, has accuracy, quick, quantitative determination's characteristics.
Description
Technical field
The utility model relates to field of medical technology, especially a kind ofly detects the residual immunofluorescent reagent box of Avermectin in beef.
Background technology
Avermectin is a kind of spectrum, efficient, safe macrolides antiparasitic agent, and current veterinary clinic is used in the domestic animals such as pig, ox, sheep widely.Avermectin is neurotoxicity compound, and by force fat-soluble, the residence time is long in animal body, and time residual excessive, bring food security hidden danger, long-term exposure on human has serious health the risk of harm, and has hypertoxicity to aquatic environment.
At present the detection method of Avermectin is mainly contained (ELISA) such as high performance liquid chromatography (HPLC), Liquid Chromatography/Mass Spectrometry (LC-MS), euzymelinked immunosorbent assay (ELISA).But the sample pre-treatments of HPLC and LC-MS method is complicated, complex operation, and this expensive equipment needed for two kinds of methods, cost is high, is unsuitable for the examination to great amount of samples.And false positive easily appears in ELISA method, cause the deviation of result.
Utility model content
The utility model is for the deficiencies in the prior art, and propose a kind ofly to detect the residual immunofluorescent reagent box of Avermectin in beef, suitable quick detection at the scene, cost is low, is suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
In order to realize above-mentioned utility model object, the utility model provides following technical scheme: a kind ofly detect the residual immunofluorescent reagent box of Avermectin in beef, and comprise case type shell, immuno-chromatographic test paper strip, this immuno-chromatographic test paper strip is located in case type shell; Described immuno-chromatographic test paper strip comprises sample pad, analyzing film, adsorptive pads, viscosity end liner and pad, this pad is coated with the anti-Avermectin monoclonal antibody of forwarding luminescent material particle marker, viscosity end liner is glued at the bottom of case type shell, analyzing film is glued at the right side of viscosity end liner, adsorptive pads is glued on the right side of analyzing film, pad right part is glued on the left of analyzing film, and sample pad is glued on the left side of pad; There is gap between adsorptive pads and pad, the analyzing film of this gap location is provided with bag by the detection zone of anti-Avermectin monoclonal antibody and bag by the quality control band of sheep anti mouse lgG; Be provided with sample added holes above case type shell, result reads window and End point indication window, sample added holes is corresponding with sample pad location, and it is corresponding with the position of detection zone and quality control band that result reads window, and End point indication window is corresponding with adsorptive pads position.
Further, the length of described analyzing film is less than viscosity end liner.
Further, described sample pad right part is glued on pad.
Further, described box case is that transparent material is made.
Compared with prior art, the utility model has the following advantages: the anti-Avermectin monoclonal antibody pad of this kit being coated with forwarding luminescent material particle marker, be provided with on analyzing film simultaneously and wrap by the detection zone of anti-Avermectin monoclonal antibody and wrap by the quality control band of sheep anti mouse lgG, Avermectin and two strain antibody reaction bonded in sample during reaction, detection zone formed Avermectin protein antibodies-Avermectin proteantigen-on forward the Avermectin protein antibodies compound of light particle marker, on turn light-emitting particles at infrared ray excited lower transmitting visible ray, radiative intensity is directly proportional to the concentration of Avermectin albumen in sample.
No matter whether there is Avermectin in sample, quality control band all can be formed sheep anti mouse-on forward the anti-Avermectin antibody complex of light particle marker, utilization forwards light immunity analysis instrument and scanning analysis is carried out to test strips, display measurement result.
This kit turns luminescence technology on adopting and is combined with immunological technique, suitable quick detection at the scene, and cost is low, is suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
Accompanying drawing explanation
Fig. 1 is the stereographic map detecting the immunofluorescent reagent box that Avermectin remains in beef;
Fig. 2 is the structural representation of immuno-chromatographic test paper strip.
In figure: 1-case type shell; 2-sample pad; 3-analyzing film; 4-adsorptive pads; 5-viscosity end liner; 6-pad; 7-detection zone; 8-quality control band; 9-sample added holes; 10-result reads window; 11-End point indication window.
Embodiment
Be described in detail the utility model below in conjunction with accompanying drawing, the description of this part is only exemplary and explanatory, should not have any restriction to protection domain of the present utility model.
The immunofluorescent reagent box that in detection beef as illustrated in fig. 1 and 2, Avermectin is residual, comprise case type shell 1 and be positioned at the immuno-chromatographic test paper strip of box case 1 inside, immuno-chromatographic test paper strip comprises sample pad 2, analyzing film 3, adsorptive pads 4, viscosity end liner 5 and be coated with the pad 6 of anti-Avermectin monoclonal antibody of forwarding luminescent material particle marker, viscosity end liner 5 gummed is fixed on the bottom of case type shell 1, analyzing film 3 is glued on viscosity end liner 5, pad 6 and adsorptive pads 4 are glued at the both sides of analyzing film 3 respectively, sample pad 2 is glued at the side of pad 6 away from analyzing film 3, analyzing film 3 is provided with and wraps by the detection zone 7 of anti-Avermectin monoclonal antibody and wrap by the quality control band 8 of sheep anti mouse lgG, case type shell 1 is provided with respectively with sample pad 2, the sample added holes 9 of analyzing film 3 and adsorptive pads 4 correspondence, result reads window 10 and End point indication window 11.
The length of analyzing film 3 is less than viscosity end liner 5; Sample pad 2 right part is glued on pad 6; Analyzing film 3 is nitrocellulose filter.Box case 1 is made up of transparent material, is convenient to observation.
The utility model detects the immunofluorescent reagent box that in beef, Avermectin is residual, in use, add the Avermectin in sample and two strain antibody reaction bonded, detection zone 7 formed Avermectin protein antibodies-Avermectin proteantigen-on forward the Avermectin protein antibodies compound of light particle marker, on turn light-emitting particles at infrared ray excited lower transmitting visible ray, radiative intensity is directly proportional to the concentration of Avermectin albumen in sample.No matter whether there is Avermectin in sample, quality control band 8 all can be formed sheep anti mouse-on forward the anti-Avermectin antibody complex of light particle marker.Utilization forwards light immunity analysis instrument and scanning analysis is carried out to test strips, display measurement result, in whole testing process, provide liquid to flow through the power of whole test strips by syphonic effect.
This kit turns luminescence technology on adopting and is combined with immunological technique, suitable quick detection at the scene, and cost is low, is suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. detect the immunofluorescent reagent box that in beef, Avermectin is residual, it is characterized in that: comprise case type shell, immuno-chromatographic test paper strip, this immuno-chromatographic test paper strip is located in case type shell; Described immuno-chromatographic test paper strip comprises sample pad, analyzing film, adsorptive pads, viscosity end liner and pad, this pad is coated with the anti-Avermectin monoclonal antibody of forwarding luminescent material particle marker, viscosity end liner is glued at the bottom of case type shell, analyzing film is glued at the right side of viscosity end liner, adsorptive pads is glued on the right side of analyzing film, pad right part is glued on the left of analyzing film, and sample pad is glued on the left side of pad; There is gap between adsorptive pads and pad, the analyzing film of this gap location is provided with bag by the detection zone of anti-Avermectin monoclonal antibody and bag by the quality control band of sheep anti mouse lgG; Be provided with sample added holes above case type shell, result reads window and End point indication window, sample added holes is corresponding with sample pad location, and it is corresponding with the position of detection zone and quality control band that result reads window, and End point indication window is corresponding with adsorptive pads position.
2. detect the immunofluorescent reagent box that in beef, Avermectin is residual as claimed in claim 1, it is characterized in that: the length of described analyzing film is less than viscosity end liner.
3. detect the immunofluorescent reagent box that in beef, Avermectin is residual as claimed in claim 1, it is characterized in that: described sample pad right part is glued on pad.
4. detect the immunofluorescent reagent box that in beef, Avermectin is residual as claimed in claim 1 or 2, it is characterized in that: described box case is that transparent material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520690822.7U CN204925130U (en) | 2015-09-09 | 2015-09-09 | Remaining immunofluorescence kit of avermectin in detection beef |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520690822.7U CN204925130U (en) | 2015-09-09 | 2015-09-09 | Remaining immunofluorescence kit of avermectin in detection beef |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204925130U true CN204925130U (en) | 2015-12-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520690822.7U Expired - Fee Related CN204925130U (en) | 2015-09-09 | 2015-09-09 | Remaining immunofluorescence kit of avermectin in detection beef |
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| CN (1) | CN204925130U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117946262A (en) * | 2024-03-26 | 2024-04-30 | 北京纳百生物科技有限公司 | Avermectin monoclonal antibody, test strip and application |
-
2015
- 2015-09-09 CN CN201520690822.7U patent/CN204925130U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117946262A (en) * | 2024-03-26 | 2024-04-30 | 北京纳百生物科技有限公司 | Avermectin monoclonal antibody, test strip and application |
| CN117946262B (en) * | 2024-03-26 | 2024-05-28 | 北京纳百生物科技有限公司 | Avermectin monoclonal antibody, test strip and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151230 Termination date: 20180909 |