CN204882565U - Detect amoxicillin's test paper - Google Patents
Detect amoxicillin's test paper Download PDFInfo
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- CN204882565U CN204882565U CN201520459045.5U CN201520459045U CN204882565U CN 204882565 U CN204882565 U CN 204882565U CN 201520459045 U CN201520459045 U CN 201520459045U CN 204882565 U CN204882565 U CN 204882565U
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- amoxicillin
- test paper
- detection line
- nitrocellulose filter
- control line
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Abstract
The utility model discloses a detect amoxicillin's test paper, the test paper lies in including supporting gasket 1 support the nitrocellulose membranes 2 on the gasket, the range upon range of connection sample gasket 4 in one end top that range upon range of connection absorbed water and fills up 3, nitrocellulose membranes 2 above nitrocellulose membranes 2's the one end, nitrocellulose membranes 2 middle part 5 peridiums of detection line have amoxicillin antigen, yi bian detection line 5 is equipped with control line 6, 6 peridiums of control line have the sheep anti -mouse antibody.
Description
Technical field
The utility model belongs to food security diagnostic field, is specifically related to a kind of test paper for detecting Amoxicillin.
Background technology
Amoxicillin (amoxicillin, AMX) residual in milk, had both caused the allergic reaction of sensitive group and the enhancing of bacterial drug resistance, and also can affect the quality and yield of dairy produce, bring heavy economic losses to dairy industry.It is that the country such as current European Union and North America is to one of essential items for inspection of import animal food that Amoxicillin remains.European Union requirements the highest limitation of Amoxicillin in milk is 4 μ g/kg.Domestic conventional method is microbial method, high performance liquid chromatography, look/matter coupling method, ELISA method etc.But the time that these methods measure, program are complicated and costly.
In view of there is no detection method fast at present, the purpose of this utility model is to provide and a kind ofly can be used for detecting fast the test paper of Amoxicillin and a kind of paper box that can be used for detecting fast Amoxicillin, and described test paper and paper box have the advantages such as convenient and swift, simple to operate, result is accurate.
Utility model content
An object of the present utility model is to provide a kind of test paper of quick detection Amoxicillin, and the test paper of quick detection Amoxicillin described in the utility model has the advantages such as convenient and swift, simple to operate, result is accurate, is suitable for clinical quick diagnosis.
The technical solution of the utility model is:
A kind of test paper for detecting Amoxicillin, comprise support pad 1, be positioned at the nitrocellulose filter 2 in described support pad 1, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter 2, above the other end, stacked connection sample pad 4, is characterized in that, described nitrocellulose filter 2 middle part is provided with detection line 5, described detection line 5 is coated with Amoxicillin antigen, detection line 5 be provided with control line 6, described control line 6 is coated with sheep anti-mouse antibody.
Further, above-mentioned test paper also comprises a shell 10 outward, only exposes the detection line 5 on sample pad 4, nitrocellulose filter 2 and control line 6.
Further, described detection line 5 and control line 6 are for be arrangeding in parallel.
Test paper described in the utility model uses in conjunction with an ELISA Plate micropore, described micropore contains the Amoxicillin antibody of gold mark, during inspection, sample pad one end of described test paper is inserted in described micropore, Amoxicillin antigen in sample first with the Amoxicillin antibody generation immune response in micropore, formed immune complex.
Test strips described in the utility model has high sensitivity and the convenient advantage detected.
The principle of work of the test strips of quick detection Amoxicillin described in the utility model is:
Adopt immune response principle, be prepared from by indirect competitive.Test paper desmoenzyme target micropore described in the utility model uses, described micropore contains the Amoxicillin antibody of gold mark, test paper is inserted in described micropore during inspection, the Amoxicillin antigen in sample first with the Amoxicillin antibody generation immune response in micropore, formed immune complex.Thereafter immune complex is along with sample chromatographic flow on NC Nitroncellulose film, when immune complex chromatography is to detection line (T detection line) on NC Nitroncellulose film, react with the resisting amoxicillin antigen be coated in advance on NC Nitroncellulose film, if the Amoxicillin in milk is more, the compound on detection line is fewer, and in the range of sensitivity that can examine, detection line does not develop the color, on the contrary, if do not have Amoxicillin in milk, then antibody and detection line react, and T line develops the color.Because control line (C line) is coated with sheep anti-mouse antibody, all can develop the color.
After reaction terminates, colloid gold immune analyser (Shenzhen Huaying Biotechnology Co., Ltd.) is utilized the optical density of control line and detection line to be analyzed, and by analyze the result obtained and carry out computing, thus obtain relative optical density number (RI).Then detector calculates according to the concentration of the typical curve be set in advance in detector to Amoxicillin and shows result, represents in units of ng/mL.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of preferred implementation of the test paper of quick detection Amoxicillin described in the utility model.
Wherein, 1: support pad, 2: nitrocellulose filter, 3: adsorptive pads, 4: sample pad, 5: detection line, 6: control line.
Fig. 2 is the structural representation of a kind of preferred implementation of the paper box of quick detection Amoxicillin described in the utility model.
Wherein, 10: shell, 4: sample pad, 5: detection line, 6: control line.
Embodiment
Be described in detail below in conjunction with the test paper of accompanying drawing to quick detection Amoxicillin described in the utility model, can not be interpreted as it is to restriction of the present utility model.The material used in following examples and reagent unless otherwise indicated, are common commercially available.
[embodiment 1] preparation detects the test paper of Amoxicillin fast
Test paper structure as shown in Figure 1, comprise support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection sample pad 4 above one end of nitrocellulose filter, described nitrocellulose filter 2 middle part detection line 5 is coated with Amoxicillin antigen, and detection line is provided with control line 6, and control line is coated with sheep anti-mouse antibody.
Wherein, nitrocellulose filter 2 for fixing envelope antigen, is also immunoreactive nidus in test paper simultaneously; Detection line 5 uses the damping fluid such as 0.01M*PBS, methyl alcohol to be diluted to the concentration of 1mg/ml Amoxicillin antigen, gets 0.7ul/cm and line on described nitrocellulose filter 2, be drying to obtain; Control line 6 is used by sheep anti-mouse antibody the damping fluid such as 0.01M*PBS, methyl alcohol to be diluted to the concentration of 1.2mg/ml, gets 0.7ul/cm and line on described nitrocellulose filter 2, be drying to obtain.
Wherein, the starting material of described sample pad 4 are hemofiltration film, do not need to process direct use.
Above-mentioned each building block is pasted onto in support pad 1 by structure shown in Fig. 1, obtains the test paper detecting Amoxicillin fast.
The method for making of gold mark micropore:
Mark Amoxicillin antibody: with 0.01MpH7.4PBS dilution, by Amoxicillin antibody dilution to 1mg/mL, add 0.2M solution of potassium carbonate simultaneously and adjust pH value, 10%BSA marks collaurum, makes golden labeling antibody.
Gold mark micropore: the golden labeling antibody 8ul/ hole that above-mentioned label taking has been remembered, adds in enzyme mark micropore, be drying to obtain.
[embodiment 2] detects Amoxicillin fast with test paper described in the utility model
Get the test paper of the preferred a kind of quick detection Amoxicillin of the utility model, as shown in Figure 1, comprise support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection sample pad 4 above the other end of nitrocellulose filter 2, described nitrocellulose filter 2 middle part detection line 5 is coated with Amoxicillin antigen, detection line is while be provided with control line 6, and control line is coated with sheep anti-mouse antibody.Detect according to the following steps:
1) take out gold mark micropore as described in example 1 above, micropore is positioned on clean desktop, draw testing sample and fully mix in micropore.
2) react in the micropore after test strips (sample pad one end) being inserted mixing.
3) interpretation, is placed in colloid gold immune analyser by described test paper, result of determination in (model HYYQ-001, Shenzhen Huaying Biotechnology Co., Ltd.)
[embodiment 3] detects Amoxicillin fast with paper box described in the utility model
Get the test paper of the preferred a kind of quick detection Amoxicillin of the utility model, as shown in Figure 1, comprise support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection sample pad 4 above the other end of nitrocellulose filter 2, described nitrocellulose filter 2 middle part detection line 5 is coated with Amoxicillin antigen, detection line is while be provided with control line 6, and control line is coated with sheep anti-mouse antibody; Described test paper also comprises a shell 10 outward as shown in Figure 2, only exposes the detection line 5 on sample pad 4, nitrocellulose filter 2 and control line 6.Detect according to the following steps:
1) take out gold mark micropore as described in example 1 above, micropore is positioned on clean desktop, draw testing sample and fully mix in micropore.
2) mixed liquor that step 1 obtains is drawn onto in sample pad, reaction.
3) interpretation, is placed in colloid gold immune analyser by described test paper, result of determination in (model HYYQ-001, Shenzhen Huaying Biotechnology Co., Ltd.).
Claims (3)
1. one kind for detecting the test paper of Amoxicillin, comprise support pad (1), be positioned at the nitrocellulose filter (2) in described support pad, stacked connection adsorptive pads (3) above one end of described nitrocellulose filter (2), stacked connection sample pad (4) above the other end, it is characterized in that, described nitrocellulose filter (2) middle part is provided with detection line (5), described detection line (5) is coated with Amoxicillin antigen, detection line (5) while be provided with control line (6), described control line (6) is coated with sheep anti-mouse antibody.
2. test paper as claimed in claim 1, it is characterized in that, described test paper also comprises a shell (10) outward, only exposes the detection line (5) on sample pad (4), nitrocellulose filter (2) and control line (6).
3. test paper as claimed in claim 2, is characterized in that, described detection line (5) and control line (6) are for be arrangeding in parallel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520459045.5U CN204882565U (en) | 2015-06-30 | 2015-06-30 | Detect amoxicillin's test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520459045.5U CN204882565U (en) | 2015-06-30 | 2015-06-30 | Detect amoxicillin's test paper |
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| Publication Number | Publication Date |
|---|---|
| CN204882565U true CN204882565U (en) | 2015-12-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520459045.5U Active CN204882565U (en) | 2015-06-30 | 2015-06-30 | Detect amoxicillin's test paper |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961128A (en) * | 2020-05-28 | 2020-11-20 | 深圳市金阅科技有限责任公司 | Amoxicillin complete antigen and preparation method thereof, and reagent strip and preparation method thereof |
-
2015
- 2015-06-30 CN CN201520459045.5U patent/CN204882565U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961128A (en) * | 2020-05-28 | 2020-11-20 | 深圳市金阅科技有限责任公司 | Amoxicillin complete antigen and preparation method thereof, and reagent strip and preparation method thereof |
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| GR01 | Patent grant |