CN104407093B - A kind of method of Glu content in quick detection fermentation liquor - Google Patents
A kind of method of Glu content in quick detection fermentation liquor Download PDFInfo
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Abstract
The present invention discloses the method for Glu content in a kind of quick detection fermentation liquor, comprises the following steps: step one, get the production bacterial strain of glutamine, and activation culture, then cultivates in culture transferring to fermentation medium; Step 2, fermentation liquor is vibrated, centrifugally obtains liquid to be measured; Step 3, preparation developping agent, developer and eluent; Step 4, drawing standard curve; Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, expansion, colour developing, wash-out, sampling, measures light absorption value with ultraviolet-visible spectrophotometer, in the typical curve that step 4 obtains, obtain respective value, namely obtain the content of Glu in fermentation liquor.Method of the present invention can detect Glu content in fermentation liquor fast, and accuracy is high, and fast simply, cost is low, ageing good.
Description
Technical field
The invention belongs to amino acid fermentation technical field, be specifically related to the method for Glu content in a kind of quick detection fermentation liquor.
Background technology
Glu is the nonessential amino acid of humans and animals, but has many distinctive physiological functions, if improve body immunity, has the special efficacies such as obvious facilitation to the performance of brain function and gastrointestinal function.Application more widely and good development prospect is had at present in fields such as health care of food, feed addictive, medical and health.The main fermentation method that adopts produces Glu both at home and abroad at present, and comparatively conventional fermentation liquor GLN detection method mainly contains automatic amino acid analyzer method, and ammonia process is surveyed in acid hydrolysis, high performance liquid chromatography, paper chromatography etc.Automatic amino acid analyzer method poor reproducibility, acid hydrolysis is surveyed ammonia process and is subject to ammonium radical ion interference in fermentation liquor, and high performance liquid chromatography cost is higher, generally need carry out column front derivation, and operation is comparatively complicated.Paper chromatography is simple to operate, and cost is lower, but the chromatography time is longer, and quantitative measurement is accurate not, and the fermenting experiment that instructs being difficult to timeliness carries out.
Summary of the invention
The object of the invention is, complicated operation, cost comparatively high-technology problem accurate for the method detecting Glu content in fermentation liquor in prior art, the method of Glu content in a kind of quick detection fermentation liquor is provided, the method can detect Glu content in fermentation liquor fast, accuracy is high, simple fast, cost is low, ageing good.
The technical scheme that the present invention realizes above-mentioned purpose employing is: a kind of method of Glu content in quick detection fermentation liquor, comprises the following steps:
Step one, get the production bacterial strain of glutamine, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 2-4min, get 1.5mL after vibration terminates and be placed in centrifuge tube centrifugal 3-5min under 10000rpm condition, obtain liquid to be measured, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.2-0.5, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.2-0.5%, obtains developer, for subsequent use; Be get the CuSO that mass concentration is 0.1% according to the volume ratio of 2:25:7-10
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/L glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 25-30min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 25-30min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
In fermentation medium described in step one, be respectively glucose 3-7%, corn steep liquor 1-3%, NH according to the content of the following nutritional labeling of mass percent
4cl2-4%, KH
2pO
40.2-0.4%, MgSO
40.08-0.2%, KCl0.1-0.2%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1-1.5g/L, FeSO
410-20mg/L, CuSO
42-10mg/L, thiamine 1-10mg/L; The inhibitor citric acid that mass percent is 0.1-0.2% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
250-150mg/L and ZnSO
410-80mg/L.
Being placed in the temperature that turbula shaker carries out the fermentation liquor vibrated described in step 2 is 50 DEG C.
beneficial effect of the present invention
First thin-layer chromatography and wash-out, rear mensuration materials absorbed light value are carried out the field of fast detection that amalyzing substances content is applied to Glu in glutamine ferment liquid by method provided by the invention first, the method can detect Glu content in fermentation liquor fast, accuracy is high, simple fast, cost is low, ageing good, overcome existing assay method not precisely, complicated operation, a cost comparatively high-technology difficult problem.
The developping agent that the present invention selects makes glutamic acid and glutamine sampling point obviously separate and without conditions of streaking, after thin-layer chromatography, first carry out drying operation, the last oven dry of colour developing can make sampling point high-visible again, effective raising degree of accuracy, have fast relative to the detection method of other routine, accurately, be easy to the advantage that operates, find that the method accuracy rate is more than 99% with the checking of HPLC repetition measurement.
Embodiment
A method for Glu content in quick detection fermentation liquor, comprises the following steps:
Step one, get the production bacterial strain of glutamine, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 2-4min, get 1.5mL after vibration terminates and be placed in centrifuge tube centrifugal 3-5min under 10000rpm condition, obtain liquid to be measured, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.2-0.5, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.2-0.5%, obtains developer, for subsequent use; Be get the CuSO that mass concentration is 0.1% according to the volume ratio of 2:25:7-10
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/L glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 25-30min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 25-30min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
In fermentation medium described in step one, be respectively glucose 3-7%, corn steep liquor 1-3%, NH according to the content of the following nutritional labeling of mass percent
4cl2-4%, KH
2pO
40.2-0.4%, MgSO
40.08-0.2%, KCl0.1-0.2%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1-1.5g/L, FeSO
410-20mg/L, CuSO
42-10mg/L, thiamine 1-10mg/L; The inhibitor citric acid that mass percent is 0.1-0.2% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
250-150mg/L and ZnSO
410-80mg/L.
Being placed in the temperature that turbula shaker carries out the fermentation liquor vibrated described in step 2 is 50 DEG C.
below in conjunction with specific embodiment, the present invention will be further described:
embodiment 1:
A method for Glu content in quick detection fermentation liquor, comprises the following steps:
Step one, get the production bacterial strain of glutamine, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use; In described fermentation medium, be respectively glucose 7%, corn steep liquor 1%, NH according to the content of the following nutritional labeling of mass percent
4cl2%, KH
2pO
40.2%, MgSO
40.08%, KCl0.1%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1.5g/L, FeSO
410mg/L, CuSO
42mg/L, thiamine 1mg/L; The inhibitor citric acid that mass percent is 0.1% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
250mg/L and ZnSO
480mg/L;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 2min, the temperature of carrying out the fermentation liquor vibrated is 50 DEG C, gets 1.5mL and is placed in centrifuge tube centrifugal 3min under 10000rpm condition, obtain liquid to be measured after vibration terminates, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.2, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.2%, obtains developer, for subsequent use; Be get the CuSO that mass concentration is 0.1% according to the volume ratio of 2:25:7
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/L glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 3min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 25min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 3min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 25min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
The acid content adopting the present embodiment to record fermentation liquor is respectively 15.62g/L, 15.68g/L and 15.64g/L, standard deviation 0.0249.Adopt HPLC method to carry out repetition measurement checking, get fermentation liquor centrifugal after supernatant is filtered, then carry out OPA and derive, loading gradient elution, analysis result is 15.58g/L.
embodiment 2:
A method for Glu content in quick detection fermentation liquor, comprises the following steps:
Step one, get the production bacterial strain of glutamine, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use; In described fermentation medium, be respectively glucose 3%, corn steep liquor 3%, NH according to the content of the following nutritional labeling of mass percent
4cl4%, KH
2pO
40.4%, MgSO
40.2%, KCl0.2%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1g/L, FeSO
420mg/L, CuSO
410mg/L, thiamine 10mg/L; The inhibitor citric acid that mass percent is 0.2% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
2150mg/L and ZnSO
410mg/L;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 4min, the temperature of carrying out the fermentation liquor vibrated is 50 DEG C, gets 1.5mL and is placed in centrifuge tube centrifugal 5min under 10000rpm condition, obtain liquid to be measured after vibration terminates, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.5, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.5%, obtains developer, for subsequent use; Be get the CuSO that mass concentration is 0.1% according to the volume ratio of 2:25:10
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/L glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 30min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 30min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
The acid content adopting the present embodiment to record fermentation liquor is respectively 30.65g/L, 30.75g/L and 30.64g/L, standard deviation 0.0411.Adopt HPLC method to carry out repetition measurement checking, get fermentation liquor centrifugal after supernatant is filtered, then carry out OPA and derive, loading gradient elution, analysis result is 30.48g/L.
embodiment 3:
A method for Glu content in quick detection fermentation liquor, comprises the following steps:
Step one, get the production bacterial strain of glutamine, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use; In described fermentation medium, be respectively glucose 5%, corn steep liquor 2%, NH according to the content of the following nutritional labeling of mass percent
4cl3%, KH
2pO
40.3%, MgSO
40.1%, KCl0.15%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1.2g/L, FeSO
415mg/L, CuSO
46mg/L, thiamine 6mg/L; The inhibitor citric acid that mass percent is 0.15% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
2100mg/L and ZnSO
445mg/L;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 3min, the temperature of carrying out the fermentation liquor vibrated is 50 DEG C, gets 1.5mL and is placed in centrifuge tube centrifugal 4min under 10000rpm condition, obtain liquid to be measured after vibration terminates, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.4, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.4%, obtains developer, for subsequent use; Be get the CuSO that mass concentration is 0.1% according to the volume ratio of 2:25:9
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/L glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 4min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 28min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 4min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 28min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
The acid content adopting the present embodiment to record fermentation liquor is respectively 31.62g/L, 31.68g/L and 31.64g/L, standard deviation 0.0206.Adopt HPLC method to carry out repetition measurement checking, get fermentation liquor centrifugal after supernatant is filtered, then carry out OPA and derive, loading gradient elution, analysis result is 31.64g/L.
Claims (3)
1. detect a method for Glu content in fermentation liquor fast, it is characterized in that: comprise the following steps:
Step one, get the production bacterial strain of Glu, after activation, access seeding tank is cultivated, and then cultivates to fermentation medium with the inoculum concentration culture transferring of 10%, obtains fermentation liquor, for subsequent use;
Step 2, get the fermentation liquor that step one obtains, be first placed in turbula shaker and vibrate 2-4min, get 1.5mL after vibration terminates and be placed in centrifuge tube centrifugal 3-5min under 10000rpm condition, obtain liquid to be measured, for subsequent use;
Step 3, get n-propanol, ammoniacal liquor and methyl alcohol according to the volume ratio of 3:1:0.2-0.5, mix, obtain developping agent, for subsequent use; Preparation mass concentration is the triketohydrindene hydrate acetone soln of 0.2-0.5%, obtains developer, for subsequent use; The CuSO that mass concentration is 0.1% is got according to the volume ratio of 2:25:7-10
4solution, ethanolic solution and mass concentration are the acetic acid solution of 10%, mix, and obtain eluent, for subsequent use;
Step 4, preparation 0.5g/L, 1g/L, 2g/L, 3g/L, 4g/L, 5g/L, 6g/L, 7g/L, 8g/L, the standard solution of 9g/L and 10g/LL-glutamine, first on thin-layer silicon offset plate, carry out point sample, each some 0.2uL, then be placed in chromatography cylinder to launch, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, again the silica gel inscribed is used 10mL elution 25-30min in test tube, then its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, drawing standard curve, for subsequent use,
Step 5, get the liquid to be measured that step 2 obtains carry out point sample on thin-layer silicon offset plate, each some 0.2uL, then be placed in chromatography cylinder and launch 45min, the developping agent that step 3 obtains is contained with in chromatography cylinder, expansion completes to be placed in baking oven dries 3-5min under 105 DEG C of conditions, dry the developer that rear sprinkling step 3 obtains to develop the color, and then thin-layer silicon offset plate is placed in baking oven under 110-120 DEG C of condition, dries 2min, oven dry terminates rear cutter and is inscribed by colour developing spot, obtain containing sour silica gel, for subsequent use;
Be placed in containing sour silica gel the eluent that 10ml step 3 obtains by obtained above, after wash-out 25-30min, sampling, its light absorption value at 515nm place is measured with ultraviolet-visible spectrophotometer, each sample determination three times, then obtain respective value in the typical curve obtained in step 4, namely obtain the content of Glu in fermentation liquor.
2. the method for Glu content in a kind of quick detection fermentation liquor as claimed in claim 1, it is characterized in that: in the fermentation medium described in step one, be respectively glucose 3-7%, corn steep liquor 1-3%, NH according to the content of the following nutritional labeling of mass percent
4cl2-4%, KH
2pO
40.2-0.4%, MgSO
40.08-0.2%, KCl0.1-0.2%; According to often liter of meter, the addition of following nutritional labeling is respectively dusty yeast 1-1.5g/L, FeSO
410-20mg/L, CuSO
42-10mg/L, thiamine 1-10mg/L; The inhibitor citric acid that mass percent is 0.1-0.2% is also added with in described fermentation medium, and by MnCl
2and ZnSO
4the activator of the glutamine synthelase of composition, and addition is respectively MnCl
250-150mg/L and ZnSO
410-80mg/L.
3. the method for Glu content in a kind of quick detection fermentation liquor as claimed in claim 1, is characterized in that: being placed in the temperature that turbula shaker carries out the fermentation liquor vibrated described in step 2 is 50 DEG C.
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| CN104880432A (en) * | 2015-06-11 | 2015-09-02 | 精晶药业股份有限公司 | Alanyl-glutamine content ultraviolet spectrometry photometric detection method |
| CN105277553B (en) * | 2015-11-23 | 2017-11-24 | 浙江农林大学 | Ninhydrin/nanometer titanium dioxide compound and its production and use |
| CN108841885A (en) * | 2018-06-28 | 2018-11-20 | 无锡晶海氨基酸股份有限公司 | A kind of method of high flux screening glutamine superior strain |
| CN111879859B (en) * | 2019-11-27 | 2021-10-08 | 江南大学 | Method for accurately detecting content of butanediamine in fermentation liquor |
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