A kind of laying out apparatus of quantitative real time PCR Instrument
Technical field
The utility model relates to PCR instrument, especially relates to a kind of laying out apparatus of quantitative real time PCR Instrument.
Background technology
The exploitation of quantitative real time PCR Instrument, different from fluorescence combination rate and obtain based on different fluorescent signal technology and air heating technology by double-stranded DNA, by the application clinically of this instrument, realize the means of tracking etc. accurately detecting, can be used for cancer chemotherapy in early days fast, reach early diagnosis and the object for the treatment of patient in time.Chinese utility model patent, number of patent application CN201020199662, disclose a kind of disk-shaped ultrahigh speed real-time fluorescent quantitative PCR instrument, comprise a casing, the heat block of predeterminable temperature of three circular distributions, a fluorescence detection device and supporting salable application of sample disk, described application of sample disk is notched disk, during setting-out, sample liquid is put into groove, then film phonograph seal is coiled by envelope, this arranges the heating surface area that effectively can increase sample liquid, realization is rapidly heated cooling, and heat conduction efficiency is high, save energy; But it is poor that this PCR instrument exists setting-out security and stability, have a strong impact on the detection perform of PCR instrument, needed improvement badly.
Summary of the invention
Problem to be solved in the utility model is to provide a kind of laying out apparatus of quantitative real time PCR Instrument, wherein the unique design of setting-out pipe, makes while facilitating injected sample liquid, improves the stability of setting-out pipe relative to setting-out disk.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is:
A laying out apparatus for quantitative real time PCR Instrument, comprises setting-out disk, is positioned over the setting-out pipe on described setting-out disk and drives the power set of setting-out disk rotational;
Described setting-out pipe comprises the pipe portion be linked in sequence, interior cap and outer cap, the bottom in described pipe portion is class spherical structure, described pipe portion internal surface and described inner cap lower surface form setting-out space, described interior cap is provided with the injection orifice towards described setting-out space, and described outer cap is provided with and stretches into the top of described injection orifice; Dop is arranged with outside described pipe portion;
The submarginal part hoop of described setting-out disk is evenly equipped with setting-out hole, and described setting-out hole outward flange is provided with draw-in groove, and described setting-out pipe is plugged in described setting-out hole, and described dop is positioned at described draw-in groove.
Further, described power set comprises motor, the transmission rod be connected with motor and is positioned at the gear on described transmission rod top;
Be provided with the teeth groove coordinated with described gear in the middle part of described setting-out disk, described setting-out disk is plugged in the top of described transmission rod by the mating reaction of described gear, teeth groove.
Further, described setting-out disk comprises edge portions and chassis portion, and the angle of described edge portions and described chassis portion is 10-50 °, and described setting-out pipe is positioned at described edge portions, and vertical with described edge portions.
Further, described chassis portion comprises some through holes.
Further, described interior cap and described pipe portion are for being threaded, and described outer cap and described interior cap are snapping, are arranged with plush copper outside described outer cap.
The advantage that the utility model has and positively effect are:
1, the application of sample pipe with class sphere bottom surface structure of the present utility model compared with existing taper bottom surface application of sample pipe be heated and area of dissipation large, heat conduction efficiency is high, is conducive to the fast lifting temperature of PCR instrument inside;
2, cover the setting of injection orifice in the utility model setting-out pipe, effectively prevent the phenomenon that the sample liquid that causes because of factors such as bore are comparatively large, pipe shaft is more shallow spills, pollutes application of sample disk, even pollutes adjacent application of sample pipe;
3, the matching design of gear and teeth groove, facilitates the grafting of setting-out disk and transmission rod, is conducive to operator and processes the forward and backward setting-out disk of reaction.
Accompanying drawing explanation
Fig. 1 is the structural representation of setting-out disk of the present utility model;
Fig. 2 is the partial enlargement structural representation in setting-out hole in Fig. 1;
Fig. 3 is the decomposition texture front view of setting-out pipe of the present utility model;
Fig. 4 is the decomposition texture upward view of setting-out pipe of the present utility model;
Fig. 5 is the one-piece construction schematic diagram of setting-out pipe of the present utility model;
Fig. 6 is the packaging assembly schematic diagram of power set of the present utility model, setting-out disk, setting-out pipe;
Fig. 7 is the Facad structure schematic diagram after the assembling of power set of the present utility model, setting-out disk, setting-out pipe;
Fig. 8 is the inverse layer structure schematic diagram after the assembling of power set of the present utility model, setting-out disk, setting-out pipe.
In figure: 1, setting-out disk, 11, setting-out hole, 111, draw-in groove, 13, teeth groove, 14, through hole, 2, setting-out pipe, 21, pipe portion, 211, dop, 22, interior cap, 221, injection orifice, 23, outer cap, 231, plush copper, 232, top, 3, power set, 31, transmission rod, 32, gear; A, edge portions, B, chassis portion.
Embodiment
Below in conjunction with accompanying drawing, specific embodiment of the utility model is elaborated.
As shown in figures 1-8, the laying out apparatus of a kind of quantitative real time PCR Instrument of the present utility model, comprises setting-out disk 1, is positioned over the setting-out pipe 2 on described setting-out disk and drives the power set 3 of setting-out disk rotational;
Described setting-out pipe 2 comprises pipe portion 21 and outer cap 23, and the bottom in described pipe portion 21 is class spherical structure; The application of sample pipe of such sphere bottom surface structure compared with existing taper bottom surface application of sample pipe be heated and area of dissipation large, heat conduction efficiency is high, is conducive to the fast lifting temperature of PCR instrument inside;
But operator are when dripping sample liquid with dropper etc., above-mentioned application of sample pipe is because of factors such as bore are comparatively large, pipe shaft is more shallow, have sample liquid to spill, pollute application of sample disk, even pollute adjacent application of sample pipe, therefore, the utility model is also provided with interior cap 22, described pipe portion 21 internal surface and described interior cap 22 lower surface form setting-out space, and described interior cap 22 is provided with the injection orifice 221 towards described setting-out space, and described outer cap is provided with and stretches into top 232 of described injection orifice 221; Namely sample liquid is entered in setting-out space by this injection orifice, avoids the appearance of the problems referred to above; In addition, this sample liquid is injected by syringe.
The submarginal part hoop of described setting-out disk 1 is evenly equipped with setting-out hole 11, and described setting-out hole 11 outward flange is provided with draw-in groove 111, is arranged with dop 211 outside described pipe portion 21; Described setting-out pipe 2 is plugged in described setting-out hole 11, and described dop 211 is positioned at described draw-in groove 111; Dop coordinates with draw-in groove, can increase the stability of setting-out pipe on setting-out disk.
Described power set 3 comprises motor, the transmission rod 31 be connected with motor and is positioned at the gear 32 on described transmission rod 31 top; Be provided with the teeth groove 13 coordinated with described gear 32 in the middle part of described setting-out disk 1, described setting-out disk 1 is plugged in the top of described transmission rod 31 by the mating reaction of described gear 32, teeth groove 13.The matching design of gear and teeth groove, facilitates the grafting of setting-out disk and transmission rod; Namely after experiment terminates, setting-out disk can directly take off from transmission rod by operator, next disk back-off is come, the bottom of application of sample pipe is gently pushed down with have gentle hands, application of sample pipe can be extruded from well, finally, clean drying treatment is carried out to used application of sample pipe, use in order to next time.
It is below the improvement of the technique scheme to the present embodiment.
Further, described setting-out disk 1 comprises edge portions A and chassis portion B, and the angle of described edge portions A and described chassis portion B is 10-50 °, and described setting-out pipe 2 is positioned at described edge portions A, and vertical with described edge portions A.
Further, described chassis portion B comprises some through holes 14, this through hole be provided with the weight being beneficial to and alleviating quadrat sampling disk, and save process raw material.
Further, described interior cap 22 and described pipe portion 21 are for being threaded, and described enclosing cover 23 and described interior cap 22 are snapping, are arranged with plush copper 231 outside described outer cap 23, and being provided with of this plush copper is beneficial to operator and opens outer cap.
Above an embodiment of the present utility model has been described in detail, but described content being only preferred embodiment of the present utility model, can not being considered to for limiting practical range of the present utility model.All equalizations done according to the utility model application range change and improve, and all should still belong within patent covering scope of the present utility model.