CN113265319B - Classification batch culture method for soil microorganisms - Google Patents
Classification batch culture method for soil microorganisms Download PDFInfo
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- CN113265319B CN113265319B CN202110665647.6A CN202110665647A CN113265319B CN 113265319 B CN113265319 B CN 113265319B CN 202110665647 A CN202110665647 A CN 202110665647A CN 113265319 B CN113265319 B CN 113265319B
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- 239000002689 soil Substances 0.000 title claims abstract description 41
- 244000005700 microbiome Species 0.000 title claims abstract description 20
- 238000012136 culture method Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 238000010438 heat treatment Methods 0.000 claims abstract description 37
- 239000008223 sterile water Substances 0.000 claims abstract description 24
- 238000001816 cooling Methods 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 230000001360 synchronised effect Effects 0.000 claims description 23
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 230000007246 mechanism Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000011403 purification operation Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
The invention discloses a soil microorganism classified batch culture method, which is characterized by comprising the following steps: including sterile water generating systems, mixing systems, and culture systems. The invention can automatically make the sterile water by a heating and rapid cooling method; the invention can realize the automatic shaking of soil and sterile water; when the sterile water is prepared, the cooling vessel can form a non-closed environment when the water is heated, but can be cooled in the closed environment when the water is cooled, so that the cooling speed is accelerated; the invention can realize that the mixed soil solution is respectively taken into culture dishes with different culture mediums.
Description
Technical Field
The invention relates to the technical field of microbial culture, in particular to a soil microbial classified batch culture method.
Background
In the process of extracting, separating and culturing microorganisms in soil, it is usually necessary to mix soil with sterile water, then inject the mixed water into culture dishes with different culture media to culture the microorganisms, and only one microorganism in each culture dish can survive and be cultured by configuring different culture media, patent No.: CN202011448388.3 discloses a microbial culture device for biopharmaceuticals, but the device mainly aims at pharmacy and only can realize filtration and purification operations.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a soil microorganism classification batch culture method, which solves the problems that the existing biological pharmacy microorganism culture device mainly aims at pharmacy and only can realize filtration and purification operations.
In order to achieve the purpose, the invention provides the following technical scheme: a classified batch culture method for soil microorganisms comprises a sterile water generation system, a mixing system and a culture system, wherein the classified culture method for the soil microorganisms comprises the following steps: the method comprises the following steps: the sterile water generating system rapidly heats and cools the water in the heating vessel through the heating mechanism and the cooling mechanism to prepare sterile water; step two: the mixing system is characterized in that a small amount of soil is put into a test tube and is uniformly mixed with the sterile water generated in the step one to form a soil solution; step three: the culture system pours the soil solution mixed in the step two into the suction dish, and the soil solution is respectively dripped into the culture dishes provided with different culture mediums through the needle cylinder, and each culture medium is respectively provided with different culture mediums, so that only specific microorganisms can survive in the culture system.
Preferably, in the first step, the sterile water generating system comprises a heating dish, a heating block is fixedly mounted at the bottom of the heating dish, a condensing pipe is arranged on the outer surface of the heating dish in a surrounding mode, a cold machine is fixedly mounted at the input end of the condensing pipe through a cold pipe interface, an insulating shell is arranged on the outer side of the heating dish, a heating base is fixedly connected to the lower surface of the insulating shell, and a through hole is formed in the lower surface of the heating base.
Preferably, a dish cover is fixedly mounted at the top end of the heat preservation shell, a through hole is formed in the middle of the dish cover, steam roofs are fixedly connected to the through hole through at least eight steam roof supports, the steam covers are slidably connected to the outer surfaces of the steam roof supports, the through hole is formed in one side of the upper surface of the dish cover, and a water plug is fixedly mounted in the through hole.
Preferably, both sides of the upper surface of the steam cover are provided with grooves, a fixture block is arranged above the grooves, the fixture block and the steam top plate are in sliding connection through a fixture block support, a spring is fixedly mounted between the opposite surfaces of the fixture block and the fixture block support, an inclined stop block is fixedly connected to the fixture block, a falling block is arranged above the inclined stop block, the falling block and the fixture block support are in sliding connection through a falling frame, and an electromagnet is fixedly mounted at the top end of the falling frame.
Preferably, the hybrid system in step two is including installing the water pump at the ware lid upper surface, the water inlet of water pump and the inside of heating ware are passed through the straw intercommunication, the below of water pump delivery port is provided with the test tube, the test tube passes through synchronous machine with the lagging casing and rotates and be connected.
Preferably, the upper left side of test tube is provided with native bucket, and the bottom of native bucket is rotated and is installed the soil baffle, one side that the test tube is close to lagging casing is provided with synchronous swivel mount, and synchronous swivel mount rotates with lagging casing to be connected, and the side fixedly connected with of synchronous swivel mount seals electric jar support, seals through sealing electric jar fixed mounting on the electric jar support and has the test tube lid, the one side that synchronous swivel mount is close to lagging casing is provided with the dog electric jar, dog electric jar fixed mounting at lagging casing's surface.
Preferably, the culture system in step three includes that the absorption ware passes through culture dish support fixed connection with lagging casing including absorbing the ware, it has three culture dish to go back fixed mounting on the culture dish support, one side fixed mounting who is close to the test tube on the absorption ware has the spout, and the top of absorbing the ware is provided with the cylinder.
Preferably, have upper and lower electric jar through cylinder support fixed mounting on the cylinder, fixed mounting has the electric jar of absorption on the upper and lower electric jar, absorbs the bottom and the inside piston fixed connection of cylinder of electric jar, the top fixedly connected with sliding block of upper and lower electric jar, sliding block and lagging casing pass through arc rack support fixed connection, fixedly connected with fan motor on the sliding block, and the output shaft fixedly connected with fan walking gear of fan motor, the last fixed surface of arc rack support is connected with the arc rack, arc rack support and arc rack toothing.
Has the advantages that:
compared with the prior art, the invention provides a soil microorganism classified batch culture method, which has the following beneficial effects: (1) the invention can automatically make the sterile water by a heating and rapid cooling method. (2) The invention can realize the automatic shaking of soil and sterile water. (3) When the invention is used for producing sterile water, the cooling vessel can form a non-closed environment when heating water, but can be cooled in a closed environment when cooling, thereby accelerating the cooling speed. (4) The invention can realize that the mixed soil solution is respectively taken into culture dishes with different culture media.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of a culture system according to the present invention;
FIG. 3 is a schematic diagram of a hybrid system according to the present invention;
FIG. 4 is a schematic diagram of a sterile water generating system according to the present invention;
FIG. 5 is a schematic view of the steam cover according to the present invention;
FIG. 6 is a schematic diagram of a structure of a fixture block according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, and fig. 6, the present invention provides a technical solution: a classified batch culture method for soil microorganisms comprises a sterile water generation system, a mixing system and a culture system, as shown in figures 4, 5 and 6, wherein the sterile water generation system comprises a heating dish 123, a heating block 124 is fixedly installed at the bottom of the heating dish 123, a condensing pipe 122 is encircled on the outer surface of the heating dish 123, a refrigerator 102 is fixedly installed at the input end of the condensing pipe 122 through a cold pipe connector 103, a heat preservation shell 109 is arranged on the outer side of the heating dish 123, a heating base 125 is fixedly connected to the lower surface of the heat preservation shell 109, a through hole is formed in the lower surface of the heating base 125, a dish cover 101 is fixedly installed at the top end of the heat preservation shell 109, a through hole is formed in the middle of the dish cover 101, at least eight steam top plate supports 119 are fixedly connected to the through hole, a steam top plate 115 is slidably connected to the outer surface of the steam top plate supports 119, a steam cover 118 is slidably connected to one side of the upper surface of the dish cover 101, a water plug 116 is fixedly installed in the through hole, grooves are formed in two sides of the upper surface of the steam cover 118, a fixture block 130 is arranged above each groove, the fixture block 130 is in sliding connection with the steam top plate 115 through a fixture block bracket 131, a spring is fixedly installed between the opposite surfaces of the fixture block 130 and the fixture block bracket 131, an inclined stop block 129 is further fixedly connected onto the fixture block 130, a falling block 128 is arranged above the inclined stop block 129, the falling block 128 is in sliding connection with the fixture block bracket 131 through a falling frame 127, and an electromagnet 126 is fixedly installed at the top end of the falling frame 127.
As shown in fig. 1 and fig. 3, the mixing system includes a water pump 121 installed on the upper surface of the dish cover 101, a water inlet of the water pump 121 is communicated with the inside of the heating dish 123 through a suction pipe 120, a test tube 112 is arranged below a water outlet of the water pump 121, the test tube 112 is rotatably connected with the heat-insulating casing 109 through a synchronous motor 114, a soil barrel 113 is arranged above the left side of the test tube 112, a soil baffle 138 is rotatably installed at the bottom end of the soil barrel 113, a synchronous rotating frame 139 is arranged on one side of the test tube 112 close to the heat-insulating casing 109, the synchronous rotating frame 139 is rotatably connected with the heat-insulating casing 109, a closed electric cylinder support 141 is fixedly connected to the side of the synchronous rotating frame 139, a test tube cover 108 is fixedly installed on the closed electric cylinder support 141 through the closed electric cylinder 142, a block electric cylinder 140 is arranged on one side of the synchronous rotating frame 139 close to the heat-insulating casing 109, and the block electric cylinder 140 is fixedly installed on the outer surface of the heat-insulating casing 109.
As shown in fig. 2, the culture system includes a suction dish 111, the suction dish 111 is fixedly connected with the heat-insulating housing 109 through a culture dish support 107, three culture dishes 106 are further fixedly mounted on the culture dish support 107, a chute 110 is fixedly mounted on one side of the suction dish 111 close to the test tube 112, a needle cylinder 117 is arranged above the suction dish 111, an upper electric cylinder 135 and a lower electric cylinder 135 are fixedly mounted on the needle cylinder 117 through a needle cylinder support 137, a suction electric cylinder 136 is fixedly mounted on the upper electric cylinder 135, the bottom end of the suction electric cylinder 136 is fixedly connected with a piston inside the needle cylinder 117, a sliding block 134 is fixedly connected with the top end of the upper electric cylinder 135, the sliding block 134 is fixedly connected with the heat-insulating housing 109 through an arc-shaped rack support 104, a fan motor 133 is fixedly connected on the sliding block 134, a fan traveling gear 132 is fixedly connected with an output shaft of the fan motor 133, an arc-shaped rack 105 is fixedly connected with the upper surface of the arc-shaped rack support 104, and the arc-shaped rack support 104 is engaged with the arc-shaped rack 105.
Discloses a soil microorganism classified batch culture method, which comprises the following steps: firstly, water is injected into a heating dish 123 through a water inlet hole on a dish cover 101, then, the heating block 124 carries out heating boiling sterilization on the water, in the heating process, steam can jack up a steam cover 118, then jack up a fixture block 130, then cross the fixture block 130, the steam cover 118 is clamped by the action of a spring after that, the steam is prevented from falling down, so that the steam is discharged, then, a cold machine 102 injects cooling water through a condensation pipe 122 to carry out rapid cooling on the heating dish 123, when the cold machine 102 is started, an electromagnet 126 is loosened to lower a falling block 128, the fixture block 130 is pushed to retract, so that the steam cover 118 falls down, the dish cover 101 is closed again, so that the heating dish 123 is rapidly cooled in a closed environment, holes are arranged on a heating base 125 and used for discharging water beads formed by rapid condensation, after the cooling is finished, a water pump 121 pumps the prepared sterile water into a test tube 112, and then, soil blocks are placed into an earth inlet barrel 113, then the synchronous motor 114 rotates to control the test tube to rotate a certain angle, so that the test tube reaches the position below the soil inlet barrel 113, then the motor rotates, the soil baffle plate 138 is controlled to be opened, soil falls into sterile water, then the synchronous motor 114 rotates to control the test tube to rotate a certain angle, so that the test tube 112 reaches the position below the test tube cover 108, then the closed electric cylinder 142 stretches and contracts to press the test tube cover 108 onto the test tube 112, then the block electric cylinder 140 is retracted, so that the synchronous rotating frame 139 can rotate synchronously with the test tube 112, then the synchronous motor 114 rotates to shake the test tube 112, so that soil and sterile water are fully shaken uniformly, then the block electric cylinder 140 extends to control the block to block the synchronous rotating frame 139, then the block electric cylinder 140 retracts, so that the test tube cover 108 is separated from the test tube 112, at the synchronous rotating frame 139 is separated from the synchronous rotating state with the test tube 112, then the synchronous motor 114 rotates to pour soil solution in the test tube 112 into the suction vessel 111 through the chute 110, then the suction electric cylinder 136 contracts to suck the soil solution into the needle cylinder 117, and then the upper electric cylinder 135 and the lower electric cylinder 135 are retracted and rotate in coordination with the fan motor 133 to drop the soil solution into each culture dish 106 one by one, wherein each culture dish 106 is provided with different culture mediums for culturing different microorganisms.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A soil microorganism classified batch culture method is characterized in that: the method comprises a sterile water generation system, a mixing system and a culture system, and the classified batch culture method of the soil microorganisms comprises the following steps:
the method comprises the following steps: the sterile water generating system rapidly heats and cools the water in the heating vessel through the heating mechanism and the cooling mechanism to prepare sterile water;
step two: the mixing system is characterized in that a small amount of soil is put into a test tube and is uniformly mixed with the sterile water generated in the step one to form a soil solution;
step three: the culture system pours the soil solution mixed in the step two into a suction dish, and the soil solution is respectively dripped into culture dishes provided with different culture mediums through a needle cylinder, and each culture dish is respectively provided with different culture mediums for culturing different microorganisms;
the sterile water generation system comprises a heating dish, a heating block is fixedly mounted at the bottom of the heating dish, a condensing pipe is wound on the outer surface of the heating dish, a cold machine is fixedly mounted at the input end of the condensing pipe through a cold pipe connector, a heat-insulating shell is arranged on the outer side of the heating dish, a heating base is fixedly connected to the lower surface of the heat-insulating shell, a through hole is formed in the lower surface of the heating base, a dish cover is fixedly mounted at the top end of the heat-insulating shell, a through hole is formed in the middle of the dish cover, a steam top plate is fixedly connected to the through hole through at least eight steam top plate supports, a steam cover is slidably connected to the outer surface of each steam top plate support, a through hole is formed in one side of the upper surface of the dish cover, and a water plug is fixedly mounted in the through hole;
the steam cover is characterized in that grooves are formed in two sides of the upper surface of the steam cover, a fixture block is arranged above each groove, the fixture block is connected with the steam top plate in a sliding mode through a fixture block support, a spring is fixedly mounted between the opposite surfaces of the fixture block and the fixture block support, an inclined stop block is further fixedly connected onto the fixture block, a falling block is arranged above the inclined stop block and is connected with the fixture block support in a sliding mode through a falling frame, and an electromagnet is fixedly mounted at the top end of the falling frame;
step two the hybrid system including installing the water pump at the ware lid upper surface, the water inlet of water pump and the inside of heating ware pass through the straw intercommunication, the below of water pump delivery port is provided with the test tube, and the test tube passes through synchronous machine with lagging casing and rotates and be connected, the upper left side of test tube is provided with native bucket, and the bottom of native bucket is rotated and is installed the soil baffle, one side that the test tube is close to lagging casing is provided with synchronous swivel mount, and synchronous swivel mount rotates with lagging casing to be connected, and the side fixedly connected with of synchronous swivel mount seals electric jar support, seals through sealing electric jar fixed mounting on the electric jar support and has the test tube lid, the one side that synchronous swivel mount is close to lagging casing is provided with the dog electric jar, dog electric jar fixed mounting is at lagging casing's surface.
2. The soil microorganism classified batch culture method according to claim 1, wherein: the culture system in step three including absorbing the ware, absorb ware and lagging casing and pass through culture dish support fixed connection, still fixed mounting has three culture dish on the culture dish support, one side fixed mounting who is close to the test tube on the absorption ware has the spout, and the top of absorbing the ware is provided with the cylinder.
3. The soil microorganism classified batch culture method according to claim 2, wherein: electric jar about having through cylinder support fixed mounting on the cylinder, fixed mounting has the electric jar of absorption on the electric jar from top to bottom, absorbs the bottom of electric jar and the inside piston fixed connection of cylinder, the top fixedly connected with sliding block of electric jar from top to bottom, sliding block and lagging casing pass through arc rack support fixed connection, fixedly connected with fan motor on the sliding block, the output shaft fixedly connected with fan walking gear of fan motor, the last fixed surface of arc rack support is connected with the arc rack, arc rack support and arc rack toothing.
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| CN209636222U (en) * | 2018-10-31 | 2019-11-15 | 贵州民族大学 | A kind of classifted training device of edaphon |
| CN110283852A (en) * | 2019-07-04 | 2019-09-27 | 漳州市农业科学研究所 | A kind of extract of soil actinomycetes fermenting liquid and preparation method thereof |
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Denomination of invention: A Batch Cultivation Method for Soil Microbial Classification Granted publication date: 20220617 Pledgee: Shandong Shanghe Huijin Village Bank Co.,Ltd. Pledgor: Shandong tianrunhe Bioengineering Co.,Ltd. Registration number: Y2024980004772 |
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